In vitro conservation protocol of Ceropegia bulbosa: An important medicinal and threatened plant species of Western Rajasthan
In vitro regeneration protocol has been standardized for highly medicinal and threatened succulent Ceropegia bulbosa Roxb. The paper focuses on morphogenic response of nodal explant when cultured on MS media. Murashige and Skoog medium supplemented with 6-benzyladenine (BA) (2.0 mgl-1) was found optimum for axillary shoot bud induction with 83.4 % response. Further shoots were multiplied through repetitive (3-4 times) transfer of the original explant and by subculture of the in vitro generated shoots. Maximum number of shoots 5.7±0.78 with shoot length of 3.6±0.82 cm was achieved on MS medium augmented with combination of 0.25 mgl-1 BA + 0.25 mgl-1 KN + 0.1 mgl-1 IAA and additives (50.0 mgl-1 ascorbic acid, 25 mgl-1 each of citric acid, arginine and adenine sulphate). For ex vitro rooting, pulse treatment of IBA 250 mgl-1 for 3 min was found optimum. The rooted shoots were successfully hardened in the green house condition (RH 75-80% at 26-28˚C) and about 80 % shoots were transferred to the garden.
Compton, M.E, and C.W Mize. 1999. Statistical considerations for in vitro research. I.Birth of an idea to collecting data. In Vitro Cell. Dev. Biol. Plant. 35, 115–121. doi: 10.1007/s11627-999-0020-2
Dhir, R., and G.vS. Shekhawat, 2014. Ecorehabilitation and biochemical studies of Ceropegia bulbosa Roxb.: a threatened medicinal succulent. Acta Physiol Plant. 36 (6): 1335-1343. doi: 10.1007/s11738-014-1512-1
Dhir, R., Shekhawat, G. S. and A. Alam. 2014. Improved Protocol for Somatic Embryogenesis and Calcium Alginate Encapsulation in Anethum graveolens L.: A Medicinal Herb. Applied Biochemistry and Biotechnology 39: 540-549 doi: 10.1007/s12010-014-1032-x
Dhir, R., and G. S. Shekhawat. 2013. Production, storability and morphogenic response of alginate encapsulated axillary meristems and genetic fidelity evaluation of in vitro regenerated Ceropegia bulbosa: a pharmaceutically important threatened plant species. Ind Crops Prod. 47: 139–144. doi: 10.1016/j.indcrop.2013.02.005
Dhir, R., and G. S. Shekhawat. 2012. Critical review on Tecomella undulata: a medicinally potent endangered plant species of Indian Thar Deseart. Int J Curr Res. 4(6) :36–44
Goyal, D., and S. Bhadauria. 2006. In vitro propagation of Ceropegia bulbosa using nodal segments. Indian Journal of Biotechnology.243(5): 565–567.
Jana, S. and G. S. Shekhawat. 2012. RAPD analysis, antioxidant study during cell differentiation and standardization of in vitro regeneration protocol of Anethum graveolens: A medicinal herb and spice. Biologia plantarum 56(1): 9-14. doi: 10.1007/s10535-012-0009-2
Jana, S. and G. S. Shekhawat. 2010. Phytochemical analysis and antibacterial screening of in vivo and in vitro extracts of Indian medicinal herb: Anethum graveolens. Research journal of medicinal Plant 4(4): 206-212. doi: 10.3923/rjmp.2010.206.212
Jana, S. and G. S. Shekhawat. 2010. Plant growth regulators, adenine sulfate and carbohydrates regulate organogenesis and in vitro flowering of Anethum graveolens. Acta Physiologiae Plantarum 33:305–311. doi: 10.1007/s11738-010-0548-0
Khan, M. A., and D. Pradhan. 2012. Antiurolithc activity of Ceropegia bulbosa extract in rats. Der Pharmacia Sinica 3(1): 148–152.
Mabberley, D. J. 1978. The plant book. Cambridge University Press, Cambridge.
Mathur, S., and G. S. Shekhawat. 2013. Establishment and characterization of Stevia rebaudiana (Bertoni) cell suspension culture: an in vitro approach for production of Steviosides. Acta Physiologiae Plantarum 35(3): 931-939 doi: 10.1007/s11738-012-1136-2
Murashige, T., and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Acta Physiol Plant 15: 473–497. doi: 10.1111/j.1399-3054.1962.tb08052.x
Nadkarni, K. M. 1976. Indian Materia Medica. Popular Prakashan, Bombay, India, 303–304
Patel, A. K., M. Phulwaria, M. K. Rai, A. K. Gupta, S. Shekhawat, and N. S. Shekhawat. 2014. In vitro propagation and ex vitro rooting of Caralluma edulis (Edgew.) Benth. & Hook. f.: an endemic and endangered edible plant species of the Thar Desert. Sci Hortic. 165: 175–180. doi: 10.1016/j.scienta.2013.10.039
Phulwaria, M., N. S. Shekhawat, J. S. Rathore, and R. P. Singh. 2013. An efficient in vitro regeneration and ex vitro rooting of Ceropegia bulbosa Roxb.—A threatened and pharmaceutical important plant of Indian Thar Desert. Ind Crops Prod. 42: 25–29. doi: 10.1016/j.indcrop.2012.05.013
Shekhawat, G. S., A. Batra, and S. Mathur. 2002. A reliable in vitro protocol for rapid mass propagation of Azadirachta indica Juss. Journal of Plant Biology 29(1): 109-112
Shekhawat, G. S., S. Mathur, and A. Batra. 2009. Role of phytohormones and various nitrogen inorganic and organic nutrients in induction of somatic embryogenesis in cell culture derived from leaflets of Azadirachta indica A. Juss. Biologia Plantarum 53 (4): 707-710. doi 10.1007/s10535-009-0127-7
Shekhawat, M. S., and N. S. Shekhawat. 2011. Micropropagation of Arnebia hispidissima (Lehm). DC. and production of alkannin from callus and cell suspension culture. Acta Physiol Plant. 33: 1445–1450. doi: 10.1007/s11738-010-0680-x
Singh, M., M. S. Rathore, D. Panwar, J. S. Rathore, H. R. Dagla, and N. S. Shekhawat. 2009 Micropropagation of selected genotype of Aloe vera L.-ancient plant for modern industry. J. Sustain. Forest. 28: 935-950. doi: 10.1080/10549810903344660
Singh, S. K., M. K. Rai, and L. Sahoo. 2012. An improved and efficient micropropagation of Eclipta alba through transverse thin cell layer culture and assessment of clonal fidelity using RAPD analysis. Industrial Crops and Products 37: 328–333. doi: 10.1016/j.indcrop.2011.12.005
Sukumar, E., G. R. Hamsaveni, and R. R. Bhima. 1996. Pharmacological actions of cerpegin, a novel pyridine alkaloid from Ceropegia juncea. Fitoterapia 66: 403–406
Swarnkar, S. and S. S., Katewa. 2008. Ethnobotanical observation on tuberous plants from tribal area of Rajasthan (India). Ethnobotanical Leaflets 12, 647-666.
Thiyagarajan, M. and P. Venkatachalam. 2012. Large scale in vitro propagation of Stevia rebaudiana (bert) for commercial application: pharmaceutically important and antidiabetic medicinal herb. Industrial Crops and Products 37: 111–117. doi: 10.1016/j.indcrop.2011.10.037
Wang, J., W. Gao, L. Zhang, and L. Huang. 2013. Establishment and quality assessment of tissue cultures in Glycyrrhiza uralensis Fisch. Appl Biochem Biotechnol. 169: 588–594. doi: 10.1007/s12010-012-0012-2
Walter, S. K. and J. Gillet. 1998. 1997 IUCN Red list of threatened plants. World Conservation Monitring Centre, Cambridge, UK
Yan, H. C., Liang, L. and Li. Y., Yang. 2010. In vitro and ex vitro rooting of Siratia grosvenorii, a traditional medicinal plant. Acta Physiol Plant. 32:115–120. doi: 10.1007/s11738-009-0386-0.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).