Tissue culture of Ophiorrhiza mungos L., a prospective method for the production of an anticancer drug, camptothecin

  • Geethu Gopinath Jawaharlal Nehru Tropical Botanic Garden and Research Institute (JNTBGRI), Palode, Thiruvananthapuram 695562, India
  • Binoy Jose Jawaharlal Nehru Tropical Botanic Garden and Research Institute (JNTBGRI), Palode, Thiruvananthapuram 695562, India
  • P Ravichandran Lab of Developmental Biology and Plant Biotechnology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi 627 412, Tirunelveli, Tamil Nadu, India
  • K Satheeshkumar Jawaharlal Nehru Tropical Botanic Garden and Research Institute (JNTBGRI), Palode, Thiruvananthapuram 695562, India

Abstract

Camptothecin (CPT), a cytotoxic quinoline indole alkaloid, is an anticancer compound. Its two major semi synthetic derivatives, topotecan and irinotecan, are Food and Drug Administration (FDA) approved drugs effectively used for treating different cancer types and sold under the trade names Hycamtin and Camptosar. Among the Ophiorrhiza species, Ophiorrhiza mungos contains the highest CPT level (0.02 % g dw). CPT level was determined in plants before flowering (0.074 ± 0.003 % g dw) and at flowering (0.052 ± 0.002 % g dw). Multiple shoot cultures were induced on seedling-derived explants of O. mungos in half strength MS solid media supplemented with 1.0 mg/L BAP to obtain 12.00 ± 1.22 shoots in 20 days. Repeated subcultures at 20 days interval yielded 20.00 ± 3.71 shoots/subculture of shoot clusters. After elongation, rooting and transplanting, the growth of shoot clusters were studied in both in vitro and same aged naturally grown seedlings. Highest biomass (4.62 ± 0.158 g fw) was obtained in in vitro-derived shoot clusters. CPT increased according to biomass and the maximum CPT was recorded in in vitro rooted shoot clusters after 15 days (0.031 ± 0.001 % g dw). Hence, in vitro-derived rooted shoot clusters of O. mungos cultivated in net-pots for 60 days under shade net house conditions found to be a sustainable source for CPT.

Downloads

Download data is not yet available.

References

1. Newsletter. Hunan 3W Botanical Extracts Inc. Main land China. 2013.

2. Viraporn V, Yamazaki M, Saito K, Denduangboripant J, Chayamarit K, Chuanasa T, Sukrong S. Correlation of camptothecin-producing ability and phylogenetic relationship in the genus Ophiorrhiza. Planta Med. 2011;77:59-64. doi: 10.1055/s-0030-1250568

3. Kumar R, Ved DK. Red listed medicinal plants of conservation concern in Southern India. FRLHT, Bangalore. 2000; 261-3.

4. Namdeo AG, Sharma A, Mahadik KR. Some observations on Nothapodytes foetida: An overview. Phcog. Rev. 2008; 12: 110-15.

5. Gunasekera SP, Badawi MM, Cordell GA, Farnsworth NR, Chitnis M. Plant anticancer agents - Isolation of camptothecin and 9-methoxycamptothecin from Ervatamia heyneana. J.Nat. Prod. 1979; 42: 475-7. doi: 10.1021/np50005a006

6. Arisawa M, Gunasekera SP, Cordell GA, Farnsworth NR. Plant anticancer agents XXI.Constituents of Merrilliodendron megacarpum. Planta Med. 1981; 4: 404-7. doi: 10.1055/s-2007-971533

7. Dai JR, Cardellina JH, Boyd MR, 20-O-beta-glucopyranosyl camptothecin from Mostueabrunonis: a potential camptothecin pro-drug with improved solubility. J. Nat. Prod. 1999; 62: 1427-9. doi: 10.1021/np990100m

8. Zhou BN, Hoch JM, Johnson RK, Mattern MR, Eng WK, Ma J, Hecht SM, Newman DJ, Kingston DG. Use of COMPARE analysis to discover new natural product drugs: isolation of camptothecin and 9-methoxycamptothecin from a new source. J. Nat. Prod. 2000; 63: 1273-6. doi: 10.1021/np000058r

9. Kulkarni AV, Patwardhan AA, Lele U, Malpathak NP. Production of camptothecin in cultures of Chonemorpha grandiflora, Pharmacognosy Res. 2010; 2: 296–9. doi: 10.4103/0976-4836.72327

10. Thriveni HN, Ravikanth G, Vasudeva R, Ganeshiah KN, Umashankar R. Camptothecin and methoxy camptothecin from callus cultures of Miquelia dentata Bedd. A rare plant of the Western Ghats of India. Ind. J. Biotechnol. 2015; 14: 123-6.

11. Kusari S, Sebastian Z, Michael S. An endophytic fungus from Camptotheca acuminata that produces camptothecin and analogues. J. Nat. Prod. 2009; 72: 2-7. doi: 10.1021/np800455b

12. Tafur S, Nelson JD, Delong DC, Svoboda GH. Antiviral components of Ophiorrhiza mungos for isolation of camptothecin and 10-methoxycamptothecin Lloydia. 1976; 39: 261-2.

13. Aimi N, Nishimura M, Miwa A, Hoshino H, Sakai S, Hagniwa J. Pumiloside and deoxy pumiloside; possible intermediate of camptothecin biosynthesis.Tetrahedron Lett.1989; 30: 4991-4. doi: 10.1016/S0040-4039(01)80563-3

14. Saito K, Sudo H, Yamasaki M, Koseki-Nakamun M, Kitajima M, Takayama H, Aimi N. Feasible production of camptothecin by hairy root cultures of Ophiorrhiza pumila. Plant Cell Rep. 2001; 20: 267-71. doi: 10.1007/s002990100320

15. Asano T, Watase I, Sudo H, Kitajima M, Takayama H, Aimi N, Yamasaki M, Saito K. Camptothecin production by in vitro cultures of Ophiorrhiza liukiuensis and O.kuroiwai. Plant Biotech. 2004; 24: 275-81. doi: 10.5511/plantbiotechnology.21.275

16. Klausmeyer P, McCloud TG, Melillo G, Scudiero DA, Cardellina JHI, Shoemaker RH. Identification of a new natural camptothecin analogue in targeted screening for HIF-1á inhibitors. Planta Med. 2007; 73: 49-52. doi: 10.1055/s-2006-951767

17. Gharpure G, Chavan B, Lele U, Hastak A, Bhave Malpure N. Camptothecin accumulation in Ophiorrhiza rugosa var. prostrata from northern Western Ghats. Curr.Sci. 2010; 98: 302–4.

18. Rani VKJ, Fijesh PV, Jose Padikkala. Micropropagation of Ophiorrhiza eriantha Wight. through leaf explant cultures. Plant Tissue Cult. Biotechnol. 2010; 20: 13-20.

19. Renjith R, Sibi CV, Rajani K, Roja G, Ramaswamy V, Krishnan S, Sabulal B. Search for Camptothecin yielding Ophiorrhiza species from southern Western Ghats in India: A HPTLC-densitometry study. Ind. Crop Prod. 2013; 43: 472–76. doi: 10.1016/j.indcrop.2012.07.054

20. Wink M. Biochemistry of plant secondary metabolism. In: Wink M (ed.) Annual Plant Reviews. Sheffield Academic Press and CRC Press. 2003; 1-16.

21. Watase I, Sudo H, Yamazaki M, Saito K. Regeneration of transformed Ophiorrhiza pumila plants producing camptothecin. Plant Biotech. 2004; 21: 337-42. doi: 10.5511/plantbiotechnology.21.337

22. NISCAIR. The Wealth of India. A dictionary of Indian Raw Materials and Industrial Products, Publication and information Directorate CSIR, New Delhi, India, 1966; 98.

23. Kirtikar KR, Basu BD. Indian Medicinal Plants (Bishen Sing Mahendrapal, New Delhi, India). 1975; 11: 1268-9.

24. Houghton PJ. Plants used to treat snakebite. ASOMPS. 1994; 3: 141.

25. Giri CC, Shyamkumar B, Anjaneyulu C. Progress in tissue culture, genetic transformation and applications of biotechnology to trees: an overview. Trees. 2004; 18:115-35. doi: 10.1007/s00468-003-0287-6

26. Roja G. Micropropagation and production of Camptothecin from in vitro plants of Ophiorrhiza rugosa var. decumbens .Nat. Prod. Res. 2008; 22: 1017 –23. doi: 10.1080/14786410802006165

27. Jose B, Satheeshkumar K. In vitro mass multiplication of Ophiorrhiza mungos Linn. Indian J. Exp. Biol. 2004;640-2.

28. Beegum AS, Martin KP, Zhang CL, Nishitha IK, Ligimol Slater A, Madhusoodanan PV. Organogenesis from leaf and internode explants of Ophiorrhiza prostrata, an anticancer drug (camptothecin) producing plant. Electron. J. Biotechnol. 2007; 10: 1-10. doi: 10.2225/vol10-issue1-fulltext-7

29. Remphery WR, Palmer CE, Blouw MJ. In vitro branching in relation to repeated subculture in two cultivars of Potentialla fruiticosa. Plant Cell Tiss.and Org. Cult. 1993; 235-40.

30. Krishnan PN, Sudha CG, Seeni S. Rapid propagation through shoot tip cultures of Trichopus zeylanicus Gaertn, a rare ethanomedicinal plant. Plant Cell Rep. 1995; 14: 708-11.

31. Sibi CV, Dintu KP, Renjith R, Krishnaraj MV, Roja G and Satheeshkumar K, A new record of Ophiorrhiza trichocarpon Blume (Rubiaceae: Ophiorrhizeae) from Western Ghats, India: Another source plant of camptothecin. J Sci Res. 2012; 4: 529-32. doi: 10.1007/BF00232652

32. Lin CS, Lin CC, Chang WC. In vitro flowering of Bambusa edulis and subsequent plantlet survival. Plant cell Tiss. and Org. Cult. 2003; 72: 71-8.

33. Hoft M, Verpoorte R, Beck E. Growth and alkaloid contents in leaves of Tabernaemontana pachysiphon Stapf (Apocynaceae) as influenced by light intensity, water and nutrient supply. Oecologia. 1996; 107: 160-9. doi: 10.1007/BF00327899

34. Elzenga G, Bruyn JW. Interrelation of alkaloid content and stage of development of 1- and 2-year-old Atropa belladonna L. Euphytica. 1956; 5: 259-66. doi: 10.1007/BF00038847

35. Roja M, Heble MR. The quinoline alkaloids camptothecin and 9 -methoxy camptothecin from tissue cultures and mature trees of Nothapodytes foetida. Phytochemistry. 1994; 36: 65-6. doi: 10.1016/S0031-9422(00)97013-4

36. Namdeo G, Priya T. Bhosale BB. Micropropagation and production of camptothecin form in vitro plants of Ophiorrhiza mungos. Asian Pac J Trop Biomed.2012; 2: S662-S666. doi: 10.1016/S2221-1691(12)60292-5

37. Roja G. Micropropagation and production of Camptothecin from in vitro plants of Ophiorrhiza rugosa var. decumbens. Nat Prod Res.2008; 22 (12):1017-23. doi: 10.1080/14786410802006165

38. Sakato K, Tanaka H, Mukai N, Misawa M. Isolation and identification of camptothecin from cell of Camptotheca acuminata suspension cultures. Agri. Bio. Chem. 1974; 38: 217-18. doi: 10.1080/00021369.1974.10861136

39. Satheeshkumar K, Seeni S. In vitro mass multiplication and production of roots in Plumbago rosea L. Planta Med. 2003; 69: 83-6. doi: 10.1055/s-2003-37035
Published
2018-01-01
How to Cite
GOPINATH, Geethu et al. Tissue culture of Ophiorrhiza mungos L., a prospective method for the production of an anticancer drug, camptothecin. Plant Science Today, [S.l.], v. 5, n. 1, p. 1-8, jan. 2018. ISSN 2348-1900. Available at: <http://horizonepublishing.com/journals/index.php/PST/article/view/359>. Date accessed: 19 jan. 2018. doi: https://doi.org/10.14719/pst.2018.5.1.359.
Section
Research Articles