Comparative Gas chromatography-mass spectrometry (GC-MS) analysis of two cultivars of Hordeum vulgare L.
DOI:
https://doi.org/10.14719/tcb.2893Keywords:
Hordeum vulgare L, Fluoride stress, GC-MS, Poaceae, Abiotic, Nitric oxideAbstract
Hordeum vulgare L. is a multi-nutritional food grain crop of the Poaceae family. It is a significant staple crop in Asia with significant nutritional and commercial importance. It is possible to grow this plant under a number of abiotic conditions of stress, such as fluoride stress. The objective of the current study was to determine whether two chosen cultivars of Hordeum vulgare could endure or tolerate fluoride stress conditions by using gas chromatography-mass spectrometry (GC-MS) analysis. The two H. vulgare cultivars’ seeds that were chosen, RD2786 and RD2794, were planted in pH-5 soil and allowed to mature for a month in clay pots. Different concentrations of fluoride (0, 5, 10, 15, 20 ppm) were added to the soil as supplements. Moreover, GC-MS was used to assess factors such as protein content and nitric oxide. Additional TLC analysis was carried out using a TLC sheet. As a result of free radical production, the cultivar RD2786 had higher total protein and nitric oxide scavenging enzyme (NOSA) activity than RD2794. Because of its osmoprotectant properties and ability to tolerate fluoride stress conditions, cultivar RD2786 of H. vulgare is therefore advised for farmers in fluoride-affected regions based on the results of this study.
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Holtekjølen AK, Uhlen AK, Bråthen E, Sahlstrøm S, Knutsen SH. Contents of starch and non-starch polysaccharides in barley varieties of different origin. Food Chem. 2006; 94(3):348-358. https://doi.org/10.1016/j.foodchem.2004.11.022
Grando S, Macpherson HG. Food barley: importance, uses and local knowledge. ICARDA, Aleppo, Syria. 2005; 1 21-37. https://hdl.handle.net/20.500.11766/67677
Lee YH, Kim JH, Kim SH, Oh JY, Seo WD, Kim KM, Jung JC, Jung YS. Barley sprouts extract attenuates alcoholic fatty liver injury in mice by reducing inflammatory response. Nutrients. 2016; 8(7):440. https://doi.org/10.3390/nu8070440
Nishiyama T, Hagiwara Y, Hagiwara H, Shibamoto T. Inhibitory effect of 2 ?-O-glycosyl isovitexin and ?-tocopherol on genotoxic glyoxal formation in a lipid peroxidation system. Food Chem Toxicol . 1994; 32(11):1047-51. https://doi.org/10.1016/0278-6915(94)90145-7
McIntosh GH, Whyte J, McArthur R, Nestel PJ. Barley and wheat foods: influence on plasma cholesterol concentrations in hypercholesterolemic men. Am J Clin Nutr. 1991; 53(5):1205-9. https://doi.org/10.1093/ajcn/53.5.1205
Wood PJ. Cereal ?-glucans in diet and health. J Cereal Sci. 2007; 46(3):230-8. https://doi.org/10.1016/j.jcs.2007.06.012
Blakeney AB, Flinn PC. Determination of non-starch polysaccharides in cereal grains with near infared reflectance spectroscopy. Mol Nutr Food Res. 2005; 49(6):546-50. https://doi.org/10.1002/mnfr.200500038
Garcia AL, Otto B, Reich SC, Weickert MO, Steiniger J, Machowetz A, Rudovich NN, Möhlig M, Katz N, Speth M, Meuser F, Doerfer J, Zunft HJ, Pfeiffer AH, Koebnick C. Arabinoxylan consumption decreases postprandial serum glucose, serum insulin and plasma total ghrelin response in subjects with impaired glucose tolerance. Eur J Clin Nutr. 2007; 61(3):334-41. https://doi.org/10.1038/sj.ejcn.1602525
Sharma P, Gujral HS. Antioxidant and polyphenol oxidase activity of germinated barley and its milling fractions. Food Chem. 2010; 120(3):673-8. https://doi.org/10.1016/j.foodchem.2009.10.059
Ullrich SE. Significance, Adaptation, Production and Trade of Barley. Book chapter in Barley Production, Improvement and Uses, Edited by Steven E. Ullrich. https://doi.org/10.1002/9780470958636.ch1
Stevens DP, McLaughlin MJ, Alston AM. Phytotoxicity of aluminium-fluoride complexes and their uptake from solution culture by Avena sativa and Lycopersicon esculentum. Plant Soil. 1997; 192(1):81-93. https://doi.org/10.1023/A:1004224526067
Jha SK, Nayak AK, Sharma YK. Fluoride toxicity effects in onion (Allium cepa L.) grown in contaminated soils. Chemosphere. 2009; 76(3):353-6. https://doi.org/10.1016/j.chemosphere.2009.03.044
Baunthiyal M, Ranghar S. Physiological and biochemical responses of plants under fluoride stress: an overview. Fluoride. 2014; 47(4): 287-293.
Kabata-Pendias A. Trace elements in soils and plants. CRC press; 2000 Nov 8. https://doi.org/10.1201/b10158
Ben-Hammouda M, Ghorbal H, Kremer RJ, Oueslatt O. Autotoxicity of barley. J Plant Nutr. 2002; 25(6):1155-61. https://www.ars.usda.gov/ARSUserFiles/50701000/cswq-0043-127169.pdf
Barbier O, Arreola-Mendoza L, Del Razo LM. Molecular mechanisms of fluoride toxicity. Chem.-Biol Interact. 2010; 188(2):319-33. https://doi.org/10.1016/j.cbi.2010.07.011
Yadu B, Chandrakar V, Keshavkant S. Responses of plants to fluoride: An overview of oxidative stress and defense mechanisms. Fluoride. 2016; 49(3):293-302.
Jackson ML. Soil chemical analysis, Pentice Hall of India Pvt. Ltd., New Delhi, India. 1973; 498:151-4.
Walkley A, Black IA. An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Sci. 1934; 37(1):29-38.
Aaman P, Graham H. Analysis of total and insoluble mixed-linked (1. fwdarw. 3),(1. fwdarw. 4)-. beta.-D-glucans in barley and oats. J Agric Food Chem. 1987; 35(5):704-9. https://doi.org/10.1021/jf00077a016
Zawawi MN, Dabwa AH, Hassan LS. Extraction of Essential Oils From Tamarind Leaves And Seed Using Microwave Extraction. J Wastes Biomass Manag. 2020; 2(2):49-52. http://doi.org/10.26480/jwbm.02.2020.49.52
Lowry O, Rosebrough N, Farr AL, Randall R. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75.
Badami S, Moorkoth S, Rai SR, Kannan E, Bhojraj S. Antioxidant activity of Caesalpinia sappan heartwood. Biol Pharm Bull. 2003; 26(11):1534-7. https://www.jstage.jst.go.jp/article/bpb/26/11/26_11_1534/_pdf
Sang J, Jiang M, Lin F, Xu S, Zhang A, Tan M. Nitric oxide reduces hydrogen peroxide accumulation involved in water stress-induced subcellular anti?oxidant defense in maize plants. J Integr Plant Biol. 2008; 50(2):231-43. http://doi.org/10.1111/j.1744-7909.2007.00594.x
Jones K, Heathcote JG. The rapid resolution of naturally occurring amino acids by thin-layer chromatography. J Chromatogr A. 1966; 24:106-11. http://doi.org/10.1016/s0021-9673(01)98107-5
Mehdi MAH, Alwai-AIA-HMA, Thabet AZA, Alarabi FYS, Omar GMN, Pradhan V. Analysis of bioactive chemical compounds of leaves extracts from Tamarindus indica using FT-IR and GC-MS spectroscopy. Asian J Res Biochem. 2020; 8(1): 22-34. http://doi.org/10.9734/ajrb/2021/v8i130171
Ansorena D, Gimeno O, Astiasarán I, Bello J. Analysis of volatile compounds by GC-MS of a dry fermented sausage: chorizo de Pamplona. Food Res Int. 2001; 34: 67–75. https://doi.org/10.1016/S0963-9969(00)00133-2
Everall NC, Lees DR. The use of barley-straw to control general and blue-green algal growth in a Derbyshire reservoir. Water Res. 1996; 30(2):269-76. https://doi.org/10.1016/0043-1354(95)00192-1
Wu HC, Bulgakov VP, Jinn TL. Pectin methylesterases: cell wall remodeling proteins are required for plant response to heat stress. Front Plant Sci. 2018; 9:1612. https://doi.org/10.3389/fpls.2018.01612
Lorenzi R, Ceccarelli N, Lercari B, Gualtieri P. Identification of retinal in higher plants: Is a rhodopsinlike protein the blue light receptor? Phytochemistry. 1994; 36(3):599-600. https://doi.org/10.1016/S0031-9422(00)89781-2
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