Acclimatory responses of seed pretreatment to modulate organic acid production associated with Krebs’ cycle in NaCl-stressed legumes
DOI:
https://doi.org/10.14719/tcb.4841Keywords:
amelioration, Krebs’cycle, legumes, salt stress, seed pretreatmentAbstract
Salinity hampers stand establishment of legumes at early growth stages that affect their yield. Seed pretreatment is an indispensable method for boosting tolerance to abiotic stresses and raising seed performance under salinity. Present study was aimed to evaluate whether seed pretreatment with mild dose of NaCl (sodium chloride) triggers ameliorative effects on growth and respiratory cycle of Cajanus cajan and Vigna mungo under salinity. Seedlings were raised hydroponically from non-pretreated and pretreated seeds under NaCl stress for three weeks. Non-pretreated Cajanus and Vigna seedlings exhibited reduction in root and shoot growth under salinity. Salt stress affected activities of enzymes associated with respiratory cycle specifically dehydrogenases, increased organic acid accumulation which played a versatile role to maintain cellular functions by balancing excess ions. Regardless of NaCl dose, the efficacy of seed pretreatment proved substantially beneficial to modulate the toxic effects of NaCl in both the test varieties. Lower perturbations of Krebs’ cycle in pretreated seedlings caused lower accumulation of organic acids due to prior seed acclimation conferring improved defence to thrive under salinity. Conclusively, since seed pretreatment contributes to minimize salt stress in both the test varieties, present study could be extended to legumes grown on soil to confirm the eco-friendly, cost-effective and ameliorative role of seed pretreatment to combat salt stress in saline prone agricultural fields.
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Copyright (c) 2025 Paramita Chatterjee, Sabarni Biswas, Baidyanath Pal, Asok Kumar Biswas
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