Trends in Current Biology

Effect of auxin on in vitro rooting of the triploid Musa paradisiaca L. cv. Nendran (AAB)

Samar Nisa, B J Radhika — Mon, 01 Jul 2024 00:00:00 -0600

Banana is an important fruit crops of the family Musaceae. Tissue culture multiplication of banana is now popular and preferred due to faster multiplication, uniformly and cost-effective production of healthy planting materials. The following work was take on to study the result of various concentrations of auxin (indole-3-butyric acid-IBA). Shoots derived from sucker explants after a series of multiplication (4 to 5), regenerated plants having 4.5 cm length were dissected out and rooted in different concentrations of auxin (IBA) (0.5 to 3.5 mg/L). Root production was started after 10 days of culture and the data taken after 20 days. From all the treatment tested all media concentrations produce roots at varied level with in a period of 15-20 days of culture. Media having concentrations of IBA were used to induce in vitro rooting in plantlets of Musa cv. Nendran after six sub cultures including elongation. The elongated shoots harvested from culture bottles were transferred to MS solid media supplemented with auxin IBA at varied concentrations. The root induction rate varied from one media concentration to another. The present study revealed that IBA (1.0 mg/L) was found to be most applicable hormone for root induction in sucker shoot tip explants of Musa cv. Nendran.

A Review of Next-Generation Sequencing Technologies and Their Impact on Clinical Research: Assessing Clinical Efficacy and Cost-Effectiveness

Tue, 02 Jul 2024 00:00:00 -0600

In contrast to microarray methods, sequence-based technologies directly determine the nucleic acid sequence. A number of modern sequencing technologies are referred to collectively as "next-generation sequencing" (NGS), often known as "high-throughput sequencing." Compared to conventional Sanger sequencing, NGS gives orders of magnitude more data at a much lower ongoing cost. These new technologies allow for much faster and more affordable sequencing of DNA and RNA, revolutionizing the study of genomics and molecular biology. Technical improvements in NGS sequencing methods have rapidly increased sequencing volume to several billion nucleotides within a short period and at a reasonable cost. Currently, NGS is developing into a molecular microscope that is permeating almost all areas of biological research. The last ten years have seen the development of NGS platforms and methodologies, and the quality of the sequences has increased to the point where NGS is now utilized in human clinical diagnosis. Due to significant cost reductions and greater community acceptance of NGS, the utilization of NGS techniques in studying clinical trials has significantly increased. NGS is a useful tool for detecting mutations in people with cancer and genetic abnormalities. To ascertain whether NGS can cost-effectively improve patient outcomes, more thorough cost-effectiveness studies of NGS applied to patient care management are required.

Typification of Litsea deccanensis (Lauraceae), a replacement name for Nees’ Tetranthera tomentosa

Anand Kumar, Partha Pratim Ghoshal, Tapas Chakrabarty — Mon, 01 Jul 2024 00:00:00 -0600

Litsea deccanensis Gamble, treated by different authors as synonyms of different names, has been recognized here as a distinct species occurring in peninsular India and Sri Lanka. Its replaced name, Tetranthera tomentosa Nees, has been lectotypified for unambiguous use of the name.