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Plant Science Today (PST)

Research communications

Vol. 3 No. 1 (2016)

Antimicrobial activity of leaf and root methanolic extracts from Vinca pusilla Murr.

DOI
https://doi.org/10.14719/pst.2016.3.1.163
Submitted
22 September 2015
Published
01-01-2016

Abstract

Vinca pusilla Murr. is a traditional medicinal plant used to treat several diseases. To substantiate the traditional medicinal utility of the plant, the present study aims at screening the antimicrobial activity of methanolic extracts of leaves and roots against five Gram positive, five Gram negative bacterial and four fungal strains. The minimum inhibitory concentration (MIC) were determined by two fold dilution assay. The results indicated that, leaf and root extracts were more effective on Bacillus subtilis and Staphylococcus aureus strains (MIC < 1 mg/mL). The tested organisms were sensitive to root extract compared to leaf extract. Fungal strains were resistant than the bacterial strains to both the extracts. Thus the present study illustrates the antimicrobial potential of the plant. 

References

  1. Abraham, J., and Dennis, T. T. 2012. Antibacterial activity of medicinal plant Cyclea peltata (Lam) Hooks & Thoms. Asian Pac J Trop Dis. 2(4): 280-284. doi: 10.1016/s2222-1808(12)60166-2 .
  2. Ankad, G. M., Upadhya, V., Pai, S. R., Roy, S. R. and Hegde, H. V. 2014. Comparative Screening of Biological Activities and Polyphenol Content in Extracts and Essential Oils of Chloroxylon swietenia DC. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. doi: 10.1007/s40011-014-0468-7.
  3. Ankad, G., Upadhya, V., Pai, S. R., Hegde, H. V., and Roy S. 2013. In vitro antimicrobial activity of Achyranthes coynei Sant. Asian Pac J Trop Dis. 3(3): 192-195. doi: 10.1016/s2222-1808(13)60039-0.
  4. Debjani, B. 1992. Effects of Growth Retardant on Growth, Flowering and Alkaloid Production in Catharanthus pusillus. Indian Forester. 118(9): 659-661.
  5. Govindasamy, C., and Srinivasan R. 2012. In vitro antibacterial activity and phytochemical analysis of Catharanthus roseus (Linn.) G. Don. Asian Pacific Journal of Tropical Biomedicine. S155-S158
  6. Kim, H. K., Blomster, R. N., Fong, H. H. S. and Farnsworth, N. R. 1970. The Isolation of Tetrahydroalstonine, Vindorosine, and Ursolic Acid from C. trichophyllus. Economic Botany. 24(1): 42-46. doi: 10.1007/bf02860633 .
  7. Mc Farland, J. 1987. Standardization of bacterial culture for disc diffusion assay. J Am Med Assoc. 49: 1176- 1178.
  8. Navitha, A., Helen Sheeba, D. A., Ramesh, C., and Sartaj Banu M. 2012. Hypoglycemic and anti-diabetic activity of ethanolic extract of Catharanthus pusillus (Murray) G. Don. IOSR Journal of Pharmacy. 2(4): 17-21. doi: 10.9790/3013-24401721 .
  9. Schmelzer, G. H., and Gurib-Fakim, A. 2008. Plant Resources of Tropical Africa I, In: Medicinal plants. Ed. PROTA Foundation, Wageningen Netherlands/ Backhuys. Publishers, Leiden, Netherlands /CTA, Wageningen., Netherlands, p 152-159.
  10. Subbaiyan, B., Samydurai P., KarthikPrabu, M., and Thangapandian V. 2014. Gas chromatography and mass spectrum analysis of Catharanthus pusillus (Murray) g. Don (Apocyanaceae). Int. Res J Pharm. App Sci. 4(2): 48-52.
  11. Subbaiyan, B., Samydurai, P., KarthikPrabu, M., Ramakrishnan, R., and Thangapandian, V. 2013. Physico-phytochemical analysis and antibacterial prospective of Catharanthus pusillus (Murray) G. Don an important anticancer medicinal plant. Int J Pharm Pharm Sci. 5(2): 212-215.
  12. Tim-Cushnie, T. P. and Lamb, J. A. 2005. Antimicrobial activity of flavonoids. Int J Antimicrobial Agents. 26(5): 343-356. doi: 10.1016/j.ijantimicag.2005.09.002 .
  13. Yadav, S. R. and Sardesai, M. M. 2002. Systematic treatment. In: Flora of Kolhapur district. Shivaji University., Kolhapur, India. p. 276.

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