Effect of physical and chemical treatments on breaking the seed dormancy of Caesalpinia bonduc (L.) Roxb.

Authors

DOI:

https://doi.org/10.14719/pst.2021.8.3.1049

Keywords:

Acid scarification, Chemical treatment, Growth hormones, Mechanical scarification, Physical treatment, seed dormancy

Abstract

Caesalpinia bonduc (L.) Roxb. is a medicinal plant belonging to the family Caesalpiniaceae was used for the present study. It is a prickly shrub widely distributed all over the world. Keeping the economic and social medicinal uses of C. bonduc seeds are being used widely in Folk, Ayurvedha, Siddha, Unani medicines to treat skin disease, eyesores, cancer, asthma, tuberculorosis, fever, toothaches etc. The aim of this study is to determine the requirements for breaking seed dormancy and germination of C. bonduc. The germination is prevented due to hard seed coat. C. bonduc seeds were experimented with various physical and chemical treatments to break the dormancy. The seeds were subjected to various treatments like mechanical scarification, dry heat method, light, hot water, acid scarification, inorganic compounds, plant growth regulators etc. The seeds treatment with mechanical scarification at 50, 40 and 30 seconds showed 100%, 80% and 10% of germination, whereas no changes was observed in dry heat. White light treatment showed 100% germination at 48 hrs, whereas darkness and red light showed least germination of about 10%. The hot-water treatment showed 100% germination. In chemical treatments, concentrated sulphuric acid scarification showed highest germination percentage, whereas lowest germination was found in nitric acid. Among the plant growth regulators, Gibberellic acid showed 100% germination whereas 2-isopentyl adenine showed least germination of 10% at 50 ppm. Results of this study prove that mechanical scarification was the most effective treatment to overcome dormancy of seeds in C. bonduc.

Downloads

Download data is not yet available.

Author Biographies

J Jaya Rathi, Department of Botany, Madras Christian College (Autonomous - Affiliated to University of Madras), Chennai 600 059, Tamil Nadu, India

Working as Assistant Professor, Department of Botany, Madras Christian College (Autonomous), Chennai 600 059, Tamil Nadu, India.  Has got 17 Years of teaching experience and her area of specialization is Phytoremediation, Bioremediation, and Ecology.

R Sasirekha, Department of Botany, Madras Christian College (Autonomous - Affiliated to University of Madras), Chennai 600 059, Tamil Nadu, India

MPhil Scholar  Department of Botany, Madras Christian College (Autonomous), Chennai

R Ranjith Kumar, Department of Botany, Madras Christian College (Autonomous - Affiliated to University of Madras), Chennai 600 059, Tamil Nadu, India

Assistant professor in Botany,  Madras Christian College (Autonomous), Chennai   , Tamil Nadu , India.   He leads research on Algal Bioprocess Technology (ABT) such as phycoremediation, Biofuel production, important microalgae species and its Industrial applications includes value added products and Environmental Biotechnology etc

References

Hilhorst HWM. A critical update on seed dormancy. I. Primary dormancy. Seed Science Research. 1995;5:61–73. https://doi.org/10.1017/S0960258500002634

Bewley JD. Seed germination and dormancy. Plant Cell 1997a:9:1055–66. https://doi.org/10.1105/tpc.9.7.1055

Li BL, Foley ME. Genetic and molecular control of seed dormancy. Trends in Plant Science. 1997;2:384–89. https://doi.org/10.1016/S1360-1385(97)90053-4

Finch-Savage WE., Leubner-Metzger G. Seed dormancy and the control of germination. New Phytol. 1997;171:505–23. https://doi.org/10.1111/j.1469-8137.2006.01787.x

Batlla D, Benech-Arnold RL. Predicting changes in dormancy level in natural seed soil banks. Plant Mol Biol. 2010;73:3–13 https://doi.org/10.1007/s11103-010-9601-z

Baskin CC, Baskin JM. Ecology, biogeography and evolution of dormancy and germination, Annals of Botany. CC Baskin; JM Baskin. (Eds.), Academic Press, San Diego, California. 1998; https://doi.org/10.1006/anbo.2000.1238

Gupta M, Mazumder UK, Kumar RS, Sivakumar T, Vamsi ML. Antitumor activity and antioxidant status of Caesalpinia bonduc against Ehrlich ascites Carcinoma in Swiss albino mice. Journal of Pharmacological Sciences. 2004;94(2):177- 84.

Singh V, Raghav PK Review on pharmacological properties of Caesalpinia bonduc L. Int J Med Arom Plants. 2012; 2(3):514-30.

Khan MR. Seed dormancy in Caesalpina bonduc (L) Roxb. Bulletin of Pure and Applied Sciences. 34-B –Botany. 2015; No.1-2:25-30.

Danthu PM, Rousesel M. Dia and Sarr A. Effect of different pre-treatment on the germination of Acacia senegal seeds. Seeds Science and Technology. 1992; 20:111-17.

Grover JK, Yadav S, Vats V. Medicinal plants of India with anti- diabetic potential. J Ethnopharmacol. 2002;81-100.

Kannur DM, Hukkeri VI, Akki KS. Antidiabetic activity of Caesalpinia bonduc seeds extracts in rats, Fitoterapia. 2006;77: 46.

Leónie Bentsink, Maarten Koornneef. Seed Dormancy and Germination. The American Society of Plant Biologist. 2008;6:14. https://dx.doi.org/10.1199%2Ftab.0119

Meher-Homji VM, Gadgil M. Ecological Diversity. In: Conservation in Developing Countries: problems and prospects, JC Daniel; JS Serrao (Eds.). Proceedings of the Centenary Seminar of the Natural History Society, Bombay Natural History Society, Oxford University Press. 1990. https://www.researchgate.net/publication/288947818.

Bewley JD, Black M. Physiology and biochemistry of seeds, Springer-Verlag, New York. 1982. https://doi.org/10.1007/978-3-642-68643-6

Sanjana Julias Thilakar and Jeya Rathi J. A study on the effect of physical and chemical treatments on breaking the dormancy of Adenanthera pavonina L. seeds. Journal of Natural Products and Plant Resources. 2013;3(5):55-63.

Porter RH. Recent developments in seed technology. The Botanical Review. 1949;15:221. https://doi.org/10.1007/BF02861694

Jeffery DJ, Holmes PM, Rebelo AG. Effects of dry heat on seed germination in selected indigenous and alien legume species in South Africa. 1988;54(1):28–34. https://sciencedirect.com/science/article/pii/S0254629916313588

Rincker CM, Agron J.Breaking of Dormancy in the Seeds of Astragalus mongholicus Bunge (Leguminosae) 1954;146:247-50. https://doi.org/10.1016/S0176-1617(11)82070-5

Zohre Zoghi, Davud Azadfar, Yahya Kooch. The effect of different treatments on seeds dormancy breaking and germination of Caspian Locust (Gleditschia caspica) Tree. Annals of Biological Research. 2011;2(5):400-06. http://scholarsresearchlibrary.com/archive.html

Brant RE, Mckee GW, Cleveland RW. Effect of chemical and physical treatment on hard seed of Penngift Crownvetch. Crop Science. 1971;11:1-6. https://doi.org/10.2135/cropsci1971.0011183X001100010001x

Abdenour Kheloufi1, LahouariaMouniaMansouri, FaizaZinebBoukhatem. Application to improve seed germination of three Acacia spp. Foresta. 2017;3:1-10. https://www.researchgate.net/deref/

Sharma Sr, Dwivedi Sk, Swarup.D. Hypoglycemic, anti hyperglycemic and hypolipidemic activities of Ceasalpinia bonduc seeds in rats. J Ethanopharmacol.1997; 58:39-44. https://doi.org/10.1016/s0378-8741(97)00079-2

Okunlola AI, Adebayo RA, Orimogunje AD. Methods of breaking seed dormancy on germination and early seedling growth of African locust bean (Parkia biglobosa) JACQ.) Benth. Journal of Horticulture and Forestry. 2011;3(1):1-6. https://doi.org/10.5897/JHF.9000076

Biswas TK, Bandopadhyay S, Mukherjee B, Sengupta BR. Oral hypogiycaemic effect of Caesalpinia bonducella, International Journal of Pharmacognosy. 2011;35(4):261. https://doi.org/10.1076/phbi.35.4.261.13304

Chakrabarti S, Biswas TK, Rokeya B, Ali L, Mosihuzzaman M, Nahar N, Khan AK. and Mukherjee B. Advanced studies on the hypoglycemic effect of Caesalpinia bonducella F. in type 1 and 2 diabetes in Long Evans rats. J Ethnopharm. 2003;84:41-46. https://doi.org/10.1016/s0378-8741(02)00262-3

Published

01-07-2021

How to Cite

1.
Jaya Rathi J, Sasirekha R, Ranjith Kumar R. Effect of physical and chemical treatments on breaking the seed dormancy of Caesalpinia bonduc (L.) Roxb. Plant Sci. Today [Internet]. 2021 Jul. 1 [cited 2024 Dec. 21];8(3):572–577. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/1049

Issue

Section

Research Articles

Most read articles by the same author(s)