Antimycotic potential of Kawayang tinik against pathogenic fungal species
DOI:
https://doi.org/10.14719/pst.2021.8.2.1093Keywords:
Bambusa blumeana, Antifungal activity, Agar well diffusion, Aspergillus niger, Penicillium chrysogenumAbstract
The importance of discovering and obtaining new, natural and sustainable sources of potential drugs have been the focus of scientific communities due to the emergence of increasing cases of microbial resistance, one of the biggest health threats in our society today. This study aimed to determine the antimycotic potential of Bambusa blumeana (kawayang tinik) specifically its leaf, rhizome, root, inner culm and outer culm extracts using the agar well diffusion assay. Results of the study revealed that all kawayang tinik extracts produced statistically equal size zone of inhibition (ZOI) against Aspergillus niger at 1 mg/ ml concentration while the ethanolic root and leaf extracts showed larger ZOI against Penicillium chrysogenum compared to other kawayang tinik extracts. Furthermore, the results of the antifungal assay showed comparable activity of kawayang tinik extracts to Fluconazole, a pharmaceutically approved antifungal drug, at 1 mg/ml concentration. Phytochemical studies further revealed the presence of alkaloids, tannins, phenols, sterols, triterpenes and flavonoids in its different parts which may support its potential antimycotic properties.
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References
Center of Disease Control and Prevention (CDC). Antibiotic/Antimivrobial resistance (AR/AMR) [Internet]. CDC 24/7; 2020 July 20 [cited 2009 Jan 8]. Available from: https://www.cdc.gov/drugresistance/index.html
World Health Organization (WHO). WHO list of bacteria for which new antibiotics are urgently needed [Internet]. WHO Online; 2017 February 27 [cited 2018 Oct 14]. Available from: https://www.who.int/news/item/27-02-2017-who-publishes-list-of-bacteria-for-which-new-antibiotics-are-urgently-needed
Stuart GU. Philippine medicinal plant: kawayang tinik [Internet]. Philippine Medicinal Plant; 2018 January [cited 2018 Nov 30]. Available from: http://www.stuartxchange.org/Kauayan.html
Tantengco OAG, Condes MLC, Estadilla HHT, Ragragio EM. Ethnobotanical survey of medicinal plants used by Ayta communities in Dinalupihan, Bataan, Philippines. Pharmacogn J. 2018; 10(5): 859-70. https://doi.org/10.5530/pj.2018.5.145
Shukla R, Sumit G, Sajal S, Dwivedi PK, Mishra A. The medicinal importance of bamboo. International J Biopharm Phytochem Research. 2012;1(1):9-15.
Kalita C, Ganguly M, Devi A. Evaluation of antioxidant capacity and antimicrobial properties of ethnic Bambuseae species and identification of the active components. International Journal of Pharmaceutical and Biological Achieves. 2016.3(1):61-71.
Nguyen PT, Xuan TD, Ha PT, Tu anh TT, Khanh TD. Inhibitory effects of bamboo leaf on the growth of Pyricularia grisea fungus. Molecular Diversity Preservation International: Agriculture [Internet]. 2018 [cited 2018 Nov 30];8(92):1-8. https://doi.org/10.3390/agriculture8070092
Mahunu GK, Zhang H, Tibiru M, Qiya A, Xiaoyun Y, Zhao ZL. Bamboo leaf flavonoid enhances the control effect of Pichia caribbica against Penicillium expansum growth and Patulin accumulation in apples. Postharvest Biology and Technology [Internet]. 2018 [cited 2019 June 30];141:1-7. https://doi.org/10.1016/j.postharvbio.2018.03.005
Patil RN, Rothe SP. Antifungal activity of some fodder plants leaf extract. International Advanced Research Journal in Science, Engineering and Technology. 2016; 3(11):205-08.
Valentino MJG, Ganado LS, Ganado MR, Undan JR. Phytochemical screening and bio assay of the anti-microbial activity of three species of bamboo in Nueva Ecija, Philippines. Advances in Environmental Biology. 2015;9(24):389-96.
Darma R, Purnamasari M, Agustina D, Pramudito TE, Sugiharti M, Suwanto A. A strong antifungal-producing bacteria from bamboo powder for biocontrol of Sclerotium rolfsii in melon (Cucumis melo var. amanta). J Plant Pathol Microbiol [Internet]. 2016 [cited 2019 June 18];7(2):1-7.https://doi.org/10.4172/2157-7471.1000334
Shen X, Zheng D, Gao J, Hou C. Isolation and evaluation of endophytic fungi with antimicrobial ability from Phyllostachys edulis. Bangladesh Journal of Pharmacology [Internet]. 2012 [cited 2019 Jan 27];7(1):249-57. https://doi.org/10.3329/bjp.v7i4.12068
Owolabi MS, Lajide L. Preliminary Phytochemical screening and antimicrobial activity of crude extracts of Bambusa vulgaris Schrad. ex J. C. Wendl. (Poaceae) from Southwestern Nigeria. American Journal of Essential Oils and Natural Products. 2015;3(1):42-45.
Wasnik DD, Tumane PM. Antibacterial activity of Bambusa bambose L. against multiple drug resistant (MDR) bacteria isolated from clinical specimen. International Journal of Pharmaceutical Sciences. 2014;25(1):215-18.
Wangawar SN, Shendarkar GR, Shelke DP, Daswad AK, Pohare JG, Roge AB. Phytochemical screening and antimicrobial activity of Dendrocalamus strictus leaves extracts. World Journal of Pharmaceutical Research. 2017;6(4):1029-41. https://doi.org/10.20959/wjpr20174-8128
Tanaka A, Zhu Q, Tan H, Horiba H, Ohnuki K, Mori Y et al. Biological activities and phytochemical profiles of extracts from different parts of Bamboo (Phyllostachys pubescens). Molecules [Internet]. 2014 [cited 2018 June 30];1(19):8238-60. https://doi.org/10.3390/molecules19068238
Roxas CA. Bamboo research in the Philippines. In: Rao AN, Ramanatha Rao V, (ed). Proceedings of Training Course cum workshop Bamboo - Conservation, Diversity, Ecogeography, Germplasm, Resource Utilization and Taxonomy; 2015 May 10; Yunnan, China. International Plant Genetic Resources Institute. ISBN: 92-9043-414-7.
Ambika K, Rajagopal B. In vitro antimicrobial and antiproliferative activity of Bambusa vulgaris. International Journal of Pharmacy and Pharmaceutical Research. 2017;9(1):10-22.
Chuah EL, Zakaria ZA, Suhaili Z, Abu bakar S and Desa MNM. Antimicrobial activities of plant extracts against Methicillin – Susceptible and Methicillin – Resistant Staphylococcus aureus. Journal of Microbiology Research [Internet]. 2014 [cited 2018 June 30];4(1):6-13. https://doi.org/10.5923/j.microbiology.20140401.02
Selvamohan T, Ramadas V, Shibila S, Kishore S. Antimicrobial activity of selected medicinal plants against some selected human pathogenic bacteria. Pelagia Research Library Advances in Applied Science Research. 2012;3(5):3374-81.
Austria KC, Waing KGW, Valentino MJ. Anti-oxidant and antibacterial potentials of Bambusa blumeana J. A. and J. H. P Schultes and Bambusa vulgaris Schrad.ex Wendl. International Journal of Biology, Pharmacy and Allied Sciences. 2017;6(11):2175-88.
Kootstra AMJ, Huurman S. Extraction of steviol glycosides from fresh Stevia using acidified water: comparison to hot water extraction including purification. Application Center for Renewable Resources - Wageningen Plant Research. 2017;1:7-22.
Sule IO, Agbabiaka TO. Antibacterial effect of some plant extracts on selected Enterobacteriaceae. Ethnobotanical Leaflets. 2008;1(2):1035-42.
Lakshmanan K, Arumungam A, Mani R. Phytochemical screening and in – vitro antimicrobial activity of Vitex negundo L. var. purpurascens Sivar. and Mold Against Pathogenic Microorganisms. Drug Invention Today. 2012;4(12):667.
Islam MA, Alam MM, Choudhury ME, Kobayashi N, Ahmed MU. Determination of minimum inhibitory concentration (MIC) of cloxacillin for selected isolates of Methicillin- resistant Staphylococcus aureus (MRSA) with their antibiogram. Bangladesh Journal of Veterinary Medicine [Internet]. 2008 [Cited 2019 Nov 24];6(1):121-26. https://doi.org/10.3329/bjvm.v6i1.1350v
Magaldi S, Mata- essayag S, Hartung de capriles C, Perez C, Colella MT, Olaizola C et al. Well diffusion for antifungal susceptibility testing. Int J Infect Dis [Internet]. 2004 [cited 2018 June 30];1(8):39-45. https://doi.org/10.1016/j.ijid.2003.03.002
Scorzoni L, Benaducci T, Ameida AMF, Silva DHS, Da silva bolzani V, Gianinni MJSM. The use of standard methodology for the determination of antifungal activity of natural products against medical yeast Candida sp and Cryptococcus sp. Brazillian Journal of Microbiology [Internet]. 2007 [cited 2018 June 30];38(1):391-97. https://doi.org/10.1590/S1517-83822007000300001
Dash AK, Elmquist WF. Fluconazole. Analytical profile of drug substances and excipients [Internet]. 2001 [cited 2019 Sept 16];27:67-113. https://doi.org/10.1016/S1075-6280(01)27005-0
Coffie GY, Antwi-Boasiako C, Darkwa NA. Phytochemical constituents of the leaves of three bamboo (Poaceae) species in Ghana. Journal of Pharmacognosy and Phytochemistry. 2014;2(6):34-38.
Auwal MS, Saka S, Mairiga IA, Sanda KA, Shuaibu A, Ibrahim A. Preliminary phytochemical and elemental analysis of aqueous and fractionated pos extracts of Acacia nilotica (Thorn mimosa). Veterinary Research Forum. 2014;5(2):95-100.
Singh R. Chemotaxonomy: a tool for plant classification. Journal of Medical Plant Studies. 2016;4(2):90-93.
Hao DC, Gu XJ, Xiao PG. 3 - Taxus medicinal resources: a comprehensive study. Medicinal Plants: Chemistry, Biology and Omics [Internet]. 2015 [cited 2018 June 30];12(2):97-136. https://doi.org/10.1016/j.chmed.2020.03.002
Zhang JJ, Liu M, Li Y, Zhou T, Xu DP. Nutritional values and biological activities of bamboo shoots and leaves. International Journal of Food and Nutrition Safety. 2016; 7(2):98-108.
Wang HX, Ng TB. Dendrocin, a distinctive antifungal protein from bamboo shoots. Biochemical and Biophysical Research Communications [Internet]. Extending the benefits of antifungal proteins from plants. [Internet]. 2003 [cited 2019 Jul 30];307(3):750-55. http://dx.doi.org/10.1016/S0006-291X(03)01229-4
Zumdahl SS, Decoste DJ. Introductory Chemistry. Cengage Learning, Inc. United States of America; 2009. p. 477.
Truong DH, Nguyen DH, Ta NTA, Bui AV, Do TH, Nguyen HC. Evaluation of the use of different solvents for phytochemical constituents, antioxidants and in vitro anti-inflammatory activities of Severinia buxifolia. Journal of Food Quality [Internet]. 2019 [cited 2019 Jul 16]; 1(1):1-9. https://doi.org/10.1155/2019/8178294
Pandey A, Tripathi S. Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug. Journal of Pharmacognosy and Phytochemistry. 2014;2(5):115-19.
Toan NP, Xuan TD, Thu ha PT, Tu anh TT, Khanh TD. Inhibitory effects of bamboo leaf on the growth of Pyricularia grisea fungus. Plant Disease Control and Agriculture [Internet]. 2018 [cited 2019 June 30];8(7):92. https://doi.org/10.3390/agriculture8070092
Cushnie T, Lamb JA. Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents [Internet]. 2005 [cited 2018 June 30];26(5):343-56. http://dx.doi.org/10.1016/j.ijantimicag.2005.09.002
Lee H, Woo ER, Lee DG. Apigenin induces cell shrinkage in Candida albicans by membrane perturbation. FEMS Yeast Research [Internet]. 2018 [cited 2019 Ju 19];18(1):1-9. https://doi.org/10.1093/femsyr/foy003
Marutescu L, Popa M, Saviuc C, Lazar V, Carmen Chifiriuc M. 8- Botanical pesticides with viricidal, bactericidal and fungicidal activity. In: Grumezescu AM, (ed). New Pesticides and Soil Sensors. Oxford, UK: Academic Press. 2017. p. 311–35. https://doi.org/10.1016/B978-0-12-804299-1.00009-6
Wafa N, Sofiane G, Mouhamed K. The antioxidant and antimicrobial activities of flavonoids and tannins extracted from Phlomis bovei De Noé. European Journal of Experimental Biology. 2016;6(3):55-61.
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