Phytochemical, pharmacological and tissue culture studies of some important species of the genus Barleria L. (Acanthaceae) - a review
DOI:
https://doi.org/10.14719/pst.2021.8.3.1117Keywords:
Acanthaceae, Metabolites, Flavonoids, Barlerin, Iridoid glycosidesAbstract
Ayurvedic sciences helped the early humans to overcome chronic dangerous diseases. There are thousands of varieties of herbs and medicinal plants used to overcome such dreadful diseases. The genus Barleria L. belongs to family Acanthaceae, a medicinally significant group of plants having diversified phytochemicals used for different pharmacological properties. It has been utilized since ancient times for medicinal purposes. It has many plant secondary metabolites such as terpenes, flavonoids, lignins, alkaloids, particularly the iridoid glycosides. The secondary metabolites extracted from Barleria spp. show potential pharmacological activities viz., anti-microbial, anti-inflammatory, anti-oxidant, anti-fertility, anti-arthritic and anti-ulcer activity. In view of these, present review is focused on the phytochemistry, pharmacology and tissue culture studies of some of the important species of the genus Barleria L.
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References
Balkwill MJ, Balkwill K. A preliminary analysis of distribution patterns in a large, pantropical genus, Barleria L. (Acanthaceae). J Biogeogr. 1998;25(1):95–110. https://doi.org/10.1046/j.1365-2699.1998.251120.x
Kumari R, Kumar S, Kumar A, Goel KK, Dubey RC. Antibacterial, antioxidant and Immuno-modulatory properties in extracts of Barleria lupulina Lindl. BMC Complement Altern Med. 2017;17(1):1–11. https://doi.org/10.1186/s12906-017-1989-4
Shendage SM, Yadav SR. Revision of the genus Barleria (Acanthaceae) in India. Rheedea. 2010;20(2):81–130.
Khan I, Jan S, Shinwari Z, Ali M, Khan Y, Kumar T. Ethnobotany and medicinal uses of folklore medicinal plants belonging to family Acanthaceae: An updated review. MOJ Biol Med. 2017;1(2):34-38. https://doi.org/10.15406/mojbm.2017.01.00009
Datta PC, Biswas C. Pharmacognostic study of the leaf and bark of Barleria prionitis Linn. Pharm Biol. 1968;8(4):1161–69. https://doi.org/10.3109/13880206809083343
Harborne J. Scutellarein 7-rhamnosylglucoside from Barleria prionitis. Phytochem Rep. 1971;10(1):2822–23.
Taneja SC, Tiwari HP. Structures of two iridoids from Barleria prionitis Linn. Tetrahedron Lett. 1975;24:1995–98.https://doi.org/10.1016/S0040-4039(00)72344-6
Gololo SS, Bassey K, Olivier MT, Agyei NM, Shai LJ, Masoko P. Isolation of an iridoid glycoside compound from the leaves of Barleria dinteri collected from Zebediela sub-region in Limpopo province, South Africa. J Pharm Sci Res. 2017;9(8):1368–72.
Kosmulalage KS, Zahid S, Udenigwe CC, Akhtar S, Ata A, Samarasekera R. Glutathione S-transferase, acetylcholinesterase inhibitory and antibacterial activities of chemical constituents of Barleria prionitis. Zeitschrift fur Naturforsch. 2007;62(4):580–86. https://doi.org/10.1515/znb-2007-0417
Ata A, Kalhari KS, Samarasekera R. Chemical constituents of Barleria prionitis and their enzyme inhibitory and free radical scavenging activities. Phytochem Lett. 2009;2(1):37–40. https://doi.org/10.1016/j.phytol.2008.11.005
Suksamrarn A. Iridoid glucosides from Barleria lupulina. J Nat Prod. 1986;49(1):179. https://doi.org/10.1021/np50043a037
Byrne LT, Sasse JM, Skelton BW, Suksamrarn A, White AH. The minor iridoid glucosides of Barleria lupulina: Isolation, crystal structure and plant growth-inhibiting properties of 6-O-Acetylshanzhiside methyl ester. Aust J Chem. 1987;40(5):785–94. https://doi.org/10.1071/CH9870785
Tuntiwachwuttikul P, Pancharoen O, Taylor WC. Iridoid glucosides of Barleria lupulina. Phytochemistry. 1998;49(1):163–66. https://doi.org/10.1016/S0031-9422(97)01049-2
Suksamrarn S, Wongkrajang K, Kirtikara K, Suksamrarn A. Iridoid glucosides from the flowers of Barleria lupulina. Planta Med. 2003;69(9):877–79. https://doi.org/10.1055/s-2003-43223
Retno W, Yasuhiro T, Tatsurou M, Suresh A, Shigetoshi K. Alkaline Phosphatase (ALP) enhancing iridoid glucosides from the Indonesian medicinal plant Barleria lupulina. Nat Prod Commun. 2010;5(1):1711–16. https://doi.org/10.1177/1934578X1000501101
Kim KH, Clardy J, Senger D, Cao S. Chakyunglupulins A and B, two novel 4,8,8-trimethylcyclooct-2-enone derivatives from Barleria lupulina. Tetrahedron Lett. [Internet]. 2015;56(21):2732–34. https://doi.org/10.1016/j.tetlet.2015.04.023
Senger DR, Hoang M V, Kim KH, Li C, Cao S. Anti-inflammatory activity of Barleria lupulina: Identification of active compounds that activate the Nrf2 cell defense pathway, organize cortical actin, reduce stress fibers, and improve cell junctions in microvascular endothelial cells. J Ethnopharmacol. [Internet]. 2016;193:397–407. https://doi.org/10.1016/j.jep.2016.09.017
Kim KH, Park YJ, Chung KH, Yip MLR, Clardy J, Senger D et al. Iridoid glycosides from Barleria lupulina. J Nat Prod. 2015;78(2):320–34. https://doi.org/10.1021/np500791a
Lee SR, Clardy J, Senger DR, Cao S, Kim KH. Iridoid and phenylethanoid glycosides from the aerial part of Barleria lupulina. Brazilian J Pharmacogn [Internet]. 2016;26(3):281–84. https://doi.org/10.1016/j.bjp.2016.01.002
Sarmad M, Mahalakshmipriya A, Senthil K. Chemical composition and in-vitro antimicrobial activity of Barleria lupulina essential oil. J Herbs Spices Med Plants. 2012;18(1):101–19. https://doi.org/10.1080/10496475.2011.653711
Gopalakrishnan S, Sthanusubramania N, Raman PV. Chemical examination of roots of Barleria buxifolia Linn. structure of Barleriaquinone. Chem Pharm Bull. [Internet]. 1984;32(10):4137–39. https://doi.org/10.1248/cpb.32.4137
Kanchanapoom T, Noiarsa P, Ruchirawat S, Kasai R, Otsuka H. Phenylethanoid and iridoid glycosides from the Thai medicinal plant, Barleria strigosa. Chem Pharm Bull. 2004;52(5):612–14. https://doi.org/10.1248/cpb.52.612
Prapalert W, Santiarworn D, Liawruangrath S, Liawruangrath B, Pyne SG. Two phenylethanoid glycosides, Parvifloroside A and B, isolated from Barleria strigosa. Chiang Mai J Sci. 2017;44(1):168–75.
Harraz FMH, El-Halawany AM, El Gayed SH, Abdel-Sattar E. Iridoid glycosides from Barleria trispinosa. Nat Prod Res. 2009;23(10):903–38. https://doi.org/10.1080/14786410802278350
Karim A, Noor AT, Malik A, Qadir MI, Choudhary MI. Barlerisides A and B, new potent superoxide scavenging phenolic glycosides from Barleria acanthoides. J Enzyme Inhib Med Chem. 2009;24(6):1332–35. https://doi/org/10.3109/14756360902887046
Karim A, Noor AT, Malik A. Structure of barlericin, the neolignan diglycoside from Barleria acanthoides. J Asian Nat Prod Res. 2010;12(8):714–18. https://doi.org/10.1080/10286020.2010.489895
Chowdhury N, Al Hasan A, Shahidullah Tareq F, Ahsan M, Zafrul Azam ATM. 4-hydroxy-trans-cinnamate derivatives and triterpene from Barleria cristata (Acanthaceae). Dhaka Univ J Pharm Sci. 2013;12(2):173–75. https://doi.org/10.3329/dujps.v12i2.17623
Jiang WL, Zhang SP, Zhu HB, Hou J. Effect of 8-O-acetyl shanzhiside methylester increases angiogenesis and improves functional recovery after stroke. Basic Clin Pharmacol Toxicol. 2011;108(1):21–27. https://doi.org/10.1111/j.1742-7843.2010.00620.x
Fan PC, Ma HP, Hao Y, He XR, Sun AJ, Jiang W, Li MX, Jing LL, He L, Ma J, Jia ZP. A new anti-fibrinolytic hemostatic compound 8-O-acetyl shanzhiside methylester extracted from Lamiophlomis rotata. J Ethnopharmacol. [Internet]. 2016;187:232–38. Available from: http://dx.doi.org/10.1016/j.jep.2016.04.016
Jiang W, Zhang S, Fu F, Zhu H, Hou J. Inhibition of nuclear factor-?B by 6-O-acetyl shanzhiside methyl ester protects brain against injury in a rat model of ischemia and reperfusion. J Neuroinflammation. 2010;7:55. https://doi.org/10.1186/1742-2094-7-55
Kaur PK, Vasisht K, Karan M. HPTLC method for shanzhiside esters: Simultaneous quantitative analysis of barlerin, acetylbarlerin and shanzhiside methyl ester in Barleria species. J Chromatogr Sep Tech. 2014;05(06). https://doi.org/10.4172/2157-7064.1000246
Rajendran J, Jacob L, Kochupappy R. Development of validated analytical methods for the simultaneous estimation of biomarkers in the leaves of Barleria cristata and bio analysis of the selected marker in plasma. J Adv Pharm Educ Res. 2014;4(4):409–16.
Amutha K, Doss DVA. Identification and antimicrobial activity of saponin fraction from the leaves of Barleria. 2012;3(10):4040–44. http://dx.doi.org/10.13040/IJPSR.0975-8232.3(10).4040-44
Dheer R, Swarnkar SK, Syeed F. Chromatographic analysis of Barleria prionitis Linn. Res J Pharm Technol. 2019;12(8):3679–86. https://doi.org/10.5958/0974-360X.2019.00628.0
Sridharan S, Chinnagounder S. Evaluation of antimicrobial activity and GC-MS profiling of Barleria montana. J Pharm Res. 2012;5(5):2921–25.
Tamil Selvi S, Jamuna S, Thekan S, Paulsamy S. Profiling of bioactive chemical entities in Barleria buxifolia L. using GC-MS analysis-a significant ethno medicinal plant. J Ayurvedic Herb Med. [Internet]. 2017;3(2):63–77.
Chen JL, Blanc P, Stoddart CA, Bogan M, Rozhon EJ, Parkinson N, Ye Z, Cooper R, Balick M, Nanakorn W, Kernan MR. New iridoids from the medicinal plant Barleria prionitis with potent activity against respiratory syncytial virus. J Nat Prod. 1998;61(10):1295–97. https://doi.org/10.1021/np980086y
Yoosook C, Panpisutchai Y, Chaichana S, Santisuk T, Reutrakul V. Evaluation of anti-HSV-2 activities of Barleria lupulina and Clinacanthus nutans. J Ethnopharmacol. 1999;67(2):179–87. https://doi.org/10.1016/s0378-8741(99)00008-2
Gupta RS, Kumar P, Dixit VP, Dobhal MP. Antifertility studies of the root extract of the Barleria prionitis Linn. in male albino rats with special reference to testicular cell population dynamics. J Ethnopharmacol. 2000;70(2):111–77. https://doi.org/10.1016/s0378-8741(99)00150-6
Unny R, Chauhan AK, Joshi YC, Dobhal MP, Gupta RS. A review on potentiality of medicinal plants as the source of new contraceptive principles. Phytomedicine. 2003;10(2–3):233–60. https://doi.org/10.1078/094471103321659997
Wanichacheewa S, Singtripop T, Sassa S, Sakamoto S, Mori T. Decrease in the number of sperm associated with decreased blood testosterone levels in male rats treated with extracts from seven plants consumed by natives of northern Thailand. Environ Toxicol Pharmacol. 2001;10(1–2):1–4. https://doi.org/10.1016/s1382-6689(00)00071-5
Verma PK, Sharma A, Joshi SC, Gupta RS, Dixit VP. Effect of isolated fractions of Barleria prionitis root methanolic extract on reproductive function of male rats: Preliminary study. Fitoterapia. 2005;76(5):428–32. https://doi.org/10.1016/j.fitote.2005.03.007
Lans C, Harper T, Georges K, Bridgewater E. Medicinal and ethnoveterinary remedies of hunters in Trinidad. BMC Complement Altern Med. 2001;1:10. https://doi.org/10.1186/1472-6882-1-10
Suba Y, Murugesan T, Kumaravelrajan R, Mandal SC, Saha BP. Antiinflammatory, analgesic and antiperoxidative efficacy of Barleria lupulina Lindl. extract. Phyther Res. 2005;19(8):695–95. https://doi.org/10.1002/ptr.1734
Wanikiat P, Panthong A, Sujayanon P, Yoosook C, Rossi AG, Reutrakul V. The anti-inflammatory effects and the inhibition of neutrophil responsiveness by Barleria lupulina and Clinacanthus nutans extracts. J Ethnopharmacol. 2008;116(2):234–44. https://doi.org/10.1016/j.jep.2007.11.035
Singh B, Bani S, Gupta DK, Chandan BK, Kaul A. Anti-inflammatory activity of “TAF” an active fraction from the plant Barleria prionitis Linn. J Ethnopharmacol. 2003;85(2–3):187–93. https://doi.org/10.1016/s0378-8741(02)00358-6
Maji AK, Bhadra S, Mahapatra S, Banerji P, Banerjee D. Mast cell stabilization and membrane protection activity of Barleria prionitis L. Pharmacogn J. [Internet]. 2011;3(24):67–71. https://doi.org/10.5530/pj.2011.24.13
Singh K, Kaur R, Singh S, Bajwa BS, Prasad DN. Anti-inflammatory activity of Barleria prionitis Linn. J Nat Remedies. 2013;13(1):1–3.
Ghule B V., Kotagale NR, Patil KS. Inhibition of the pro-inflammatory mediators in rat neutrophils by shanzhiside methyl ester and its acetyl derivative isolated from Barleria prionitis. J Ethnopharmacol. [Internet]. 2020;249:112374. https://doi.org/10.1016/j.jep.2019.112374
Juvekar A, Gambhire M, Wankhede S. Antiinflammatory activity of aqueous extract of Barleria cristata leaves. J Young Pharm. 2009;1(3):220-24. https://doi.org/10.4103/0975-1483.57068
Mazumder PM, Sasmal D, Choudhary RK. Wound healing potential of the leaf extracts of Barleria cuspidata Heyne ex Nees. Pharmacologyonline. 2009;1:357–62.
Sridharan S, Meenaa V, Kavitha J, Brindha P, Sasikumar C. Barleria montana wight and nees – a promising natural anti-inflammatory agent against formalin induced inflammation. Int J Pharm Pharm Sci. 2015;7(9):80–84.
Suba V, Murugesan T, Rao RB, Pal M, Mandal SC, Saha BP. Neuropharmacological profile of Barleria lupulina Lindl. extract in animal models. J Ethnopharmacol. 2002;81(2):251–55. https://doi.org/10.1016/s0378-8741(02)00087-9
Amoo SO, Ndhlala AR, Finnie JF, Van Staden J. Antifungal, acetylcholinesterase inhibition, antioxidant and phytochemical properties of three Barleria species. South African J Bot. [Internet]. 2011;77(2):435–45. https://doi.org/10.1016/j.sajb.2010.11.002
Atif M, Rahman SA, Ahmed MI, Mahmood SB, Azharuddin M. Anticataract potential of Barleria prionitis: In vivo study. Int J Pharm Pharm Sci. 2015;7(2):100–55.
Sawarkar HA, Kashyap PP, Kaur CD. RBC haemolysis prevention and antioxidant activity of Barleria prionitis. Chiang Mai J Sci. 2018;45(2):888–96.
Yadav SA, Raj AJ, Sathishkumar R. In vitro antioxidant activity of Barleria noctiflora L. f. Asian Pac J Trop Biomed. [Internet]. 2012;2(2 SUPPL.):S716–22. https://doi.org/10.1016/S2221-1691(12)60302-5
Narmada R, Devaki K. In vitro antioxidant activity and invitro ?-glucosidase and ? -amylase inhibitory activity of Barleria cristata L. Res J Pharm Biol Chem Sci. 2012;3(4):780–88.
Tamboli F, More H. Evaluation of antiulcer and antioxidant activity of Barleria gibsonii Dalz. leaves. Pharmacognosy Res. 2016;8(4):226–30. https://doi.org/10.4103/0974-8490.188879
Mazumder PM, Mondal A, Sasmal D, Arulmozhi S, Rathinavelusamy P. Evaluation of antiarthritic and immunomodulatory activity of Barleria lupulina. Asian Pac J Trop Biomed. [Internet]. 2012;2(3 SUPPL.):S1400–6. https://doi.org/10.1016/S2221-1691(12)60425-0
Ghule B V., Yeole PG. In vitro and in vivo immunomodulatory activities of iridoids fraction from Barleria prionitis Linn. J Ethnopharmacol. [Internet]. 2012;141(1):424–31. https://doi.org/10.1016/j.jep.2012.03.005
Johnson Inbaraj J, Krishna MC, Gandhidasan R, Murugesan R. Cytotoxicity, redox cycling and photodynamic action of two naturally occurring quinones. Biochim Biophys Acta - Gen Subj. 1999;1472(3):462–70. https://doi.org/10.1016/s0304-4165(99)00150-6
Manglani N, Vaishnava S, Dhamodaran P, Sawarkar H. In vitro and in vivo anti-cancer activity of leaf extract of Barleria grandiflora. Int J Pharm Pharm Sci. 2014;6(3):70–72.
Choudhary M, Kumar V, Gupta PK, Singh S. Anti-arthritic activity of Barleria prionitis Linn. leaves in acute and chronic models in Sprague Dawley rats. Bull Fac Pharmacy, Cairo Univ. [Internet]. 2014;52(2):199–209. https://doi.org/10.1016/j.bfopcu.2014.07.002
Suba V, Muragesan T, Pal M, Mandal SC, Saha BP. Antiulcer activity of methanol fraction of Barleria lupulina Lindl. in animal models. Phyther Res. 2004;18(11):925–29. https://doi.org/10.1002/ptr.1587
Jaiswal SK, Dubey MK, Das S, Rao C V. Gastroprotective effect of the iridoid fraction from Barleria prionitis leaves on experimentally-induced gastric ulceration. Chin J Nat Med. [Internet]. 2014;12(10):738–44. https://doi.org/10.1016/S1875-5364(14)60113-8
Suba V, Murugesan T, Arunachalam G, Mandal SC, Saha BP. Anti-diabetic potential of Barleria lupulina extract in rats. Phytomedicine. 2004. https://doi.org/10.1016/s0367-326x(03)00163-1
Dheer R, Bhatnagar P. A study of the antidiabetic activity of Barleria prionitis Linn. Indian J Pharmacol. 2010;42(2):70–73. https://doi.org/10.4103/0253-7613.64493
Arumugam S, Natesan S. Hypoglycemic effects of Barleria noctiflora fractions on high fat fed with low dose Streptozotocin induced type-2 diabetes in rats. Int J Pharm Pharm Sci. 2016;8(2):193–200.
Vasanth S, Bupesh G, Vijayakumar TS, Balachandar V, Gunasekaran DR. Evaluation of in vitro antidiabetic and antioxidant potential of Barleria cristata leaves extracts. Asian J Pharm Clin Res. 2018;11(4):287–90. https://doi.org/10.22159/ajpcr.2018.v11i4.24069
Singh B, Chandan BK, Prabhakar A, Taneja SC, Singh J, Qazi GN. Chemistry and hepatoprotective activity of an active fraction from Barleria prionitis Linn. in experimental animals. Phyther Res. 2005;19(5):391–404. https://doi.org/10.1002/ptr.1509
Banu S, Arunachalam G, Jayaveera KN, Babu VA, Kumar V. Hepatoprotective activity of methanolic extract of Barleria montana leaves in ethanol treated rats. Asian Pacific J Trop Dis. [Internet]. 2012;2(SUPPL2):S748–52. https://doi.org/10.1016/S2222-1808(12)60257-6
Tulliballi S, Seru G. Phytochemical investigation and evaluation of hepatoprotective and antimicrobial activities on the aerial parts of Barleria montana (Acanthaceae). Rasayan J Chem. 2013;6(2):102–16.
Amit K, Shiwani S, Rajesh K, Rajinder K, Singh LK, Shilpa K. Pharmacognostical, preliminary phytochemical screening and antimicrobial studies of leaves of Barleria prionitis Linn. Int J Pharmacogn Phytochem Res. 2014;6(2):369–78.
Yuliastuti F, Hapsari WS, Syarifuddin A, Septianingrum NMAN, Pradani MPK. Fractionation antibiotic isolate: Trial protocol for antibiotic compound from Barleria prionitis L. extract. Technol Reports Kansai Univ. 2020;62(3):989–94.
Natarajan D, Gomathi M, Yuvarajan R. Phytochemical and antibacterial evaluation of Barleria montana Nees. Academic Sciences. Asian J Pharm Clin Res. 2011;5(3):44–46.
Kumari S, Jain P, Sharma B, Kadyan P, Dabur R. In vitro antifungal activity and probable fungicidal mechanism of aqueous extract of Barleria grandiflora. Appl Biochem Biotechnol. 2015;175(8):3571–84. https://doi.org/10.1007/s12010-015-1527-0
Pal A, Kumar R, Upadhyay L, Tripathi YC. Antifungal activity of natural dye from aerial biomass of Barleria prionitis L. and dyed fabrics. Iran J Chem Chem Eng. 2018;37(1):213–21. https://doi.org/10.30492/IJCCE.2018.30706
Jaiswal SK, Dubey MK, Verma AK, Das S, Vijaykumar M, Rao C V. Evaluation of iridoid glycosides from leaves of Barleria prionitis as an anti-diarrhoeal activity: An ethnopharmacological study. Int J Pharm Sci. 2010;2(3):680–86.
Marya BH, Bothara SB. Investigation of antihypertensive activity of leaves of Barleria prionotis in doca salt induced hypersensitive rats. Int J Pharma Sci Res. 2013;18(2):17–19.
Wang C, Gong X, Bo A, Zhang L, Zhang M, Zang E et al. Iridoids: Research advances in their phytochemistry, biological activities, and pharmacokinetics. Molecules. 2020;25(2):287. https://doi,org/10.3390/molecules25020287
Devi N, Parashar B, Thakur R. Comparative, evaluation parameter for toothpaste?: A Review. 2019;03(06):57–64.
Kotteswari M, Krishna Rao MR, Prabhu K, Kumar S, Shil S. Antioxidant studies of one ayurvedic medicine Aswagandharishtam. Asian J Pharm Clin Res. 2018;11(11):227–31. https://doi.org/10.22159/ajpcr.2018.v11i11.27593
Vador N, Vador B, Rajgor N. Anti-oxidant and anti-arthritic potential of ayurvedic formulations: Maharasnadi quath extract and Stifain tablet. Indian J Pharm Pharmacol. 2020;7(1):43–47. ?http://doi.org/10.18231/j.ijpp.2020.009
Lone SA, Yadav AS, Badkhane Y, Sharma AK, Bakhshi SH, Raghuwanshi DK. Effect of different plant growth regulators on in-vitro propagation of Barleria prionitis L.- a threatened medicinal plant. Int J Pharma Bio Sci. 2011;2(1):438–44.
Lone SA, Yadav AS, Bajaj A, Sharma AK, Badkhane Y, Raghuwanshi DK. Conservation strategies for threatened medicinal plant - Barleria prionitis L. using in vitro and ex vitro propagation techniques. Arch Phytopathol Plant Prot. 2012;45(11):1327–40. https://doi.org/10.1080/03235408.2012.673301
Singh R, Arya S, Arora K, Chodhurary M, Dev I. Micropropagation of Barleria prionitis L. var. dicantha?: an ethnomedicinal plant. Adv For Sci. 2015;2(24):73–78. https://doi.org/10.34062/afs.v2i4.2651
Kumar S, Rani A. Micropropagation of vajradanti (Barleria prionitis L.): a high value antidontalgic and ethno-medicinal herb. J Phytol Res. 2016;29:1–6.
Soni V, Kumari R, Swarnkar PL. High frequency in vitro regeneration system for conservation of Barleria prionitis L., a threatened medicinal shrub. Bot Pacifica. 2017;6(1):45–49. https://doi.org/ 10.17581/bp.2017.06104
Shukla P, Singh A, Gawri S AA. and SS. In vitro propagation of Barleria prionitis Linn. and its antibacterial activity. Int J Pharma Prof Res. 2011;2:198–200.
Moin S, Babu SS, Mahalakshmipriya A. In vitro callus production and antibacterial activity of Barleria lupulina Lindl. Asia-Pacific J Mol Biol Biotechnol. 2012;20(2):59–64.
Kumari P, Yadav P, Arya A, Kumar S. In vitro callus production and anti-bacterial activity of Barleria prionitis Linn. against dental caries pathogens. Int J Bot Res. 2013;3(4):1–6.
Kumar S. Establishment of callus cultures and their Physico- Biochemical analysis in Barleria lupulina Lindl. Intern Res Jour Managt Socio Human. 2018;9(11):244–51. https://doi.org/10.32804/IRJMSH
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