Identification and characterization of phytoconstituents of ethanolic root extract of Clitoria ternatea L. utilizing HR-LCMS analysis
DOI:
https://doi.org/10.14719/pst.2021.8.3.1141Keywords:
Central nervous system, Ayurvedic formulation, phytochemical screening, Medicinal plantsAbstract
Medicinal plants act as a vital source in improving health and overcoming the side effects of modern-day medicine. Many evidence-based reports are present in the literature about the benefits of medicinal plants. Clitoria ternatea L. belongs to the family Fabaceae and is known to be one of the important Ayurvedic medicinal plant whose uses are specified mainly for the modification of nervous system activities. ‘Medhyarasayana’ is one of the Ayurvedic formulations which is used to promote the intellectual capacity, revive the body and nervous tissue, Clitoria ternatea serves as a major constituent of ‘Medhyarasayana.’ Identification and characterization of active metabolites of C. ternatea will help to isolate the important phytoconstituents responsible for the central nervous system effects, isolated components can be utilized in future for the formulation of new medicine for various neurodegenerative disorders. In the present study, the phytochemical evaluation of the ethanolic root extract of C. ternatea (EECT) was performed using the HR-LCMS technique. Preliminary qualitative phytoconstituents analysis showed the presence of tannins, alkaloids, saponins, steroids, carbohydrate, protein, flavonoids and triterpenoids in the ethanolic root extract. Almost 42 compounds were identified when the EECT subjected to HR-LCMS analysis.
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Gratus C, Damery S, Wilson S, Warmington S, Routledge P, Grieve R, Steven N, Jones J, Greenfield S. The use of herbal medicines by people with cancer in the UK: a systematic review of the literature. Q J Med.2009;102:831-42. https://doi.org/10.1093/qjmed/hcp137
Taylor S, Berridge V. Medicinal plants and malaria: an historical case study of research at the London School of Hygiene and Tropical Medicine in the twentieth century. Trans R Soc Trop Med Hyg. 2006;100(8):707-14. https://doi.org/10.1016/j.trstmh.2005.11.017
Vickers A, Zollman C. ABC of complementary medicine herbal medicine. BMJ. 1999;319:1050-53. http://doi.org/10.1136/bmj.319.7216.1050
Fikrat IA. Cancer chemopreventive and tumoricidal properties of Saffron (Crocus sativus L.). Exp Biol Med. 2002;227:20-25. https://doi.org/10.1177/153537020222700104
Singh RH, Narsimhamurthy K, Singh G. Neuronutrient impact of Ayurvedic Rasayana therapy in brain ageing. Biogerontology. 2008; 9:369-74. https://doi.org/10.1007/s10522-008-9185-z
Jiji KN. Muralidharan P. Neuropharmacological Potential of Clitoria ternatea Linn. - A Review. Research J Pharm and Tech. 2020;13(11):1-6. https://doi.org/10.5958/0974-360X.2020.00960.9
Manjula P, Mohan CH, Sreekanth D. Phytochemical analysis of Clitoria ternatea (Linn.): A valuable medicinal plant. Journal of Indian Botanical Society. 2013; 92:(3&4):173-78.
Mukherjee PK, Kumar V, Mal M, Houghton PJ. Acetyl cholinesterase inhibitors from plants. Phytomedicine. 2007; 14:289-300. https://doi.org/10.1016/j.phymed.2007.02.002
Jain NN, Ohal CC, Shroff SK, Bhutada RH, Somani RS, Kasture VS. Clitorea ternatea and the CNS. Pharmacol Biochem Behav. 2003; 75:529-36.
Parimaladevi B, Boominathan R, Mandal SC. Evaluation of antipyretic potential of Clitorea ternatea L. extract in rats. Phytomedicine. 2004;11:323-26. https://doi.org/10.1016/s0367-326x(03)00057-1
Kulkarni C, Pattanshetty JR, Amruthraj G. Effect of alcoholic extract of Clitorea ternatea Linn. on central nervous system in rodents. Indian J Exp Biol. 1988; 26:957-60. PMID: 3254867
Nadkarni KM. Indian Materia Medica. 3rd ed. Bombay: Popular Publication;1976. p.20-45.
Daisy P, Rajathi M. Hypoglycemic effects of Clitorea ternatea Linn. (Fabaceae) in alloxan induced diabetes in rats. Trop J Pharm Res. 2009;8:393-98.
Yadav RNS, Agarwal M. Phytochemical analysis of some medicinal plants. Journal of Phytology. 2011;3(12):10-14.
Pulok K Mukherjee. Quality Control and Evaluation of Herbal Drugs – Evaluating Natural Products and Traditional Medicine. Elsevier; 2019. p.79-149.
Digambar SP, Jyoti G, Sahera N. Antimicrobial activity and HR-LCMS analysis of methanolic extract of Calotropis gigantea. International Journal of Advanced Science and Research. 2019;4(1):19-24.
Patil AP, Patil RV. Clitoria ternatea Linn.: An overview. IJPSR. 2011;3:20-23.
Rai KS. Neurogenic potential of Clitoria ternatea aqueous root extract–a basis for enhancing learning and memory. Int J Pharm Sci Rev Res. 2010;4:186-91. https://doi.org/10.5281/zenodo.1074966
Taranalli AD, Cheeramkuzhy TC. Influence of Clitoria ternatea extracts on memory and central cholinergic activity in rats. Pharm Biol. 2000;38(1):51-56. https://doi.org/10.1076/1388-0209(200001)3811-BFT051
Rai KS, Murthy KD, Karanth KS, Nalini K, Rao MS. Clitoria ternatea root extract enhances acetylcholine content in rat hippocampus. Fitoterapia. 2002;73:685-89. https://doi.org/10.1016/S0367-326X(02)00249-6
Margret AA, Begum TN, Parthasarathy S, Suvaithenamudhan S. A strategy to employ Clitoria ternatea as a prospective brain drug confronting monoamine oxidase (MAO) against neurodegenerative diseases and depression. Nat prod Bioprospect. 2015;5:293-306. https://doi.org/10.1007/s13659-015-0079-x
Parvathi M, Ravishankar K. Evaluation of antidepressant, motor coordination and locomotor activities of ethanolic root extract of Clitoria ternatea. J Nat Remedies. 2013;13:19-24. https://doi.org/10.18311/jnr/2013/113
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