Phytochemical analysis and antioxidant activities of Artemisia stelleriana Besser leaf extracts

Authors

DOI:

https://doi.org/10.14719/pst.1263

Keywords:

Artemisia stelleriana, Total phenolic content, Antioxidant activity, DPPH

Abstract

The present study aims to report the proximate and mineral composition, phenolic contents, and antioxidant potential of Artemisia stelleriana leaves. The leaf extracts were prepared using various solvents like distilled water, methanol, ethanol, and acetone and analyzed for their phenolic and flavonoid contents and antioxidant activity. The methanolic extracts showed the highest total phenolic and flavonoid contents (10.09 ± 0.24 mg GAE/g and 225.04 ± 0.38 mg QE/ g, respectively). The methanolic extracts showed significantly higher 1,1-Diphenyl-2-picrylhydrazyl radical scavenging assay (DPPH-RSA), Reducing power assay, and total antioxidant capacity compared to distilled water, ethanol, and acetone extracts. Gas Chromatography-Mass Spectroscopy revealed that the methanolic extracts of leaves to be a good source of bioactive compounds like 2,4-di-tert-butylphenol (2,4-DTBP), neophytadiene, octacosane, and eucalyptol.

Downloads

Download data is not yet available.

References

Atanasov AG, Waltenberger B, Pferschy-Wenzig EM, Linder T, Wawrosch C, Uhrin P, Temml V, Wang L, Schwaiger S, Heiss EH, Rollinger JM. Discovery and resupply of pharmacologically active plant-derived natural products: A review. Biotechnol Adv 2015; 33(8):1582-614. https://doi.org/10.1016/j.biotechadv.2015.08.001

Chen L, Cao H, Xiao J. Polyphenols: Absorption, bioavailability, and metabolomics. In Polyphenols: properties, recovery, and applications 2018; pp. 45-67. Woodhead Publishing. https://doi.org/10.1016/B978-0-12-813572-3.00002-6

Kondratyuk TP, Pezzuto JM. Natural product polyphenols of relevance to human health. Pharma Biol 2004; 42(sup1):46-63. https://doi.org/10.3109/13880200490893519

Sefi M, Fetoui H, Makni M, Zeghal N. Mitigating effects of antioxidant properties of Artemisia campestris leaf extract on hyperlipidemia, advanced glycation end products and oxidative stress in alloxan-induced diabetic rats. Food Chem Toxicol 2010; 48(7):1986-1993. DOI: 10.1016/j.fct.2010.05.005.https://doi.org/10.1016/j.fct.2010.05.005

Chiasson H, Belanger A, Bostanian N, Vicent C, Poliquin A. Acaricidal properties of Artemisia absinthium and Tanacetum vulgare (Asteraceae) essential oils obtained by three methods of extraction. J Econ Entmol 2001; 94(1): 167-171. DOI: 10.1603/0022-0493-94.1.167.https://doi.org/10.1603/0022-0493-94.1.167

Mojab F. Antimalarial natural products: a review. Avicenna J Phytomedicine. 2012; 2(2):52.

Bilia AR, Santomauro F, Sacco C, Bergonzi MC, Donato R. Essential oil of Artemisia annua L.: an extraordinary component with numerous antimicrobial properties. Evid based Complementary Altern Med 2014; 1. DOI: 10.1155/2014/159819.https://doi.org/10.1155/2014/159819

Alesaeidi S, Miraj S. A systematic review of anti-malarial properties, immunosuppressive properties, anti-inflammatory properties, and anti-cancer properties of Artemisia annua. Electronic physician. 2016; 8(10):3150. DOI: 10.19082/3150.https://doi.org/10.19082/3150

Wiart C. Ethnopharmacology of medicinal plants: Asia and the Pacific. Springer Science & Business Media; 2007; pp-14-98.https://doi.org/10.1201/9781420006803

Ali N, Shah SW, Shah I. Preliminary phytochemical screening and antispasmodic activity of Artemisia macrocephala Jacquem. J Young Pharm 2011; 3(2):125-8. DOI: 10.4103/0975-1483.80300.https://doi.org/10.4103/0975-1483.80300

Amidon C, Barnett R, Cathers J, Chambers B, Hamilton L, Kellett A, Kennel E, Montowski J, Thomas MA, Watson B. Artemisia: An Essential Guide from The Herb Society of America. Caroline, A., Thomas, M., Kennel, E., Eds. 2014.

Couplan F, Duke J. The encyclopedia of edible plants of North America. McGraw Hill Professional; 1998.

Shen LX, Tsering T, Geng YP, Zhong Y, Nan P. Chemical constituents of essential oils from six Artemisia species in Tibet and their antibacterial activity. Journal of Fudan University (Natural Science). 2010;1.

Padalia RC, Verma RS, Chauhan A, Goswami P, Chanotiya CS. Essential oil composition of Artemisia stelleriana Besser from India. J Essent Oil Res 2016; 28(5):400-405. DOI: 10.1080/10412905.2016.1175978.https://doi.org/10.1080/10412905.2016.1175978

Carvalho IS, Teixeira MC, Brodelius M. Fatty acids profile of selected Artemisia spp. plants: Health promotion. LWT-Food Sci Technol 2011; 44(1): 293-298.https://doi.org/10.1016/j.lwt.2010.05.033

Joseph KS, Bolla S, Joshi K, Bhat M, Naik K, Patil S, Bendre S, Gangappa B, Haibatti V, Payamalle S, Shinde S. Determination of chemical composition and nutritive value with fatty acid compositions of African mangosteen (Garcinia livingstonei). Erwerbs-Obstbau 2017; 59(3):195-202. https://doi.org/10.1007/s10341-016-0311-9

Arnon DI. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 1949; 24(1): 1.https://doi.org/10.1007/s10341-016-0311-9

Cunniff P, Horwitz W. Official Methods of Analysis of the AOAC International, volume 2, Food Composition, Additives, and Natural Contaminants. 2000.https://doi.org/10.1104/pp.24.1.1

Hinneburg I, Dorman HD, Hiltunen R. Antioxidant activities of extracts from selected culinary herbs and spices. Food Chem 2006;97(1):122-9. https://doi.org/10.1016/j.foodchem.2005.03.028

Carvalho IS, Cavaco T, Brodelius M. Phenolic composition and antioxidant capacity of six artemisia species. Ind Crops Prod 2011; 33(2): 382-8. https://doi.org/10.1016/j.indcrop.2010.11.005

Murthy HN, Joseph KS, Gaonkar AA, Payamalle S. Evaluation of chemical composition and antioxidant activity of Cordia myxa fruit pulp. J Herbs Spices Med Plants 2019; 25(3):192-201. https://doi.org/10.1080/10496475.2019.1585399

Essiett AU, Udo ES. Comparative phytochemical screening and nutritional potentials of the stems, leaves and flowers of Allamanda Cathartica (Apocynaceae). Int J Sci Technol 2015; 4:248-53.

Ogunnowo AA, Alao-Sanni O. Comparative antioxidant, phytochemical and proximate analysis of aqueous and methanolic extracts of Vernonia amygdalina and Talinum triangulare. Pakistan J Nutr 2010; 9(3): 259-64. DOI: 10.3923/pjn.2010.259.264.https://doi.org/10.3923/pjn.2010.259.264

Song X, Wen X, He J, Zhao H, Li S, Wang M. Phytochemical components and biological activities of Artemisia argyi. J Funct Foods. 2019; 52:648-62. https://doi.org/10.1016/j.jff.2018.11.029

Mensah JK, Okoli RI, Ohaju-Obodo JO, Eifediyi K. Phytochemical, nutritional and medical properties of some leafy vegetables consumed by Edo people of Nigeria. Afr J Biotechnol 2008; 7(14).

Jahnen-Dechent W, Ketteler M. Magnesium basics. Clin Kidney J 2012; 5(Suppl_1): i3-14.https://doi.org/10.1093/ndtplus/sfr163

Campbell JD. Lifestyle, minerals and health. Medical hypotheses. 2001; 57(5): 521-31. DOI: 10.1054/mehy.2001.1351.https://doi.org/10.1054/mehy.2001.1351

Alothman M, Bhat R, Karim AA. Antioxidant capacity and phenolic content of selected tropical fruits from Malaysia, extracted with different solvents. Food Chem 2009; 115(3): 785-8. DOI: 10.1016/j.foodchem.2008.12.005.https://doi.org/10.1016/j.foodchem.2008.12.005

Ahmed MI, Xu X, Sulieman AA, Mahdi AA, Na Y. Effect of extraction conditions on phenolic compounds and antioxidant properties of koreeb (Dactyloctenium aegyptium) seeds flour. J Food Meas Charact. 2020;14(2):799-808. DOI: 10.1007/s11694-019-00328-9.https://doi.org/10.1007/s11694-019-00328-9

Aftab A, Yousaf Z, Aftab ZE, Younas A, Riaz N, Rashid M, Shamsheer HB, Razzaq Z, Javaid A. Pharmacological screening and GC-MS analysis of vegetative/reproductive parts of Nigella sativa L. Pakistan Journal of Pharmaceutical Sciences. 2020; 33(5): 2103-2111. DOI: 10.36721/PJPS.2020.33.5.REG.2103-2111.1.https://doi.org/10.36721/PJPS.2020.33.5.REG.2103-2111.1

Iqbal S, Younas U, Chan KW, Zia-Ul-Haq M, Ismail M. Chemical composition of Artemisia annua L. leaves and antioxidant potential of extracts as a function of extraction solvents. Molecules 2012; 17(5):6020-32. DOI: 10.3390/molecules17056020.https://doi.org/10.3390/molecules17056020

Kim DO, Lee CY. HPLC separation of polyphenolics. Current protocols in food analytical chemistry 2002; 6(1): I1-3. https://doi.org/10.1002/0471142913.fai0103s06

Ashraf A, Sarfraz RA, Mahmood A. Phenolic compounds’ characterization of Artemisia rutifolia spreng from Pakistani flora and their relationships with antioxidant and antimicrobial attributes. Int J Food Prop 2017; 20(11):2538-49. https://doi.org/10.1080/10942912.2016.1243556

Amensour M, Sendra E, Pérez-Alvarez JA, Skali-Senhaji N, Abrini J, Fernández-López J. Antioxidant activity and chemical content of methanol and ethanol extracts from leaves of rockrose (Cistus ladaniferus). Plant Foods Hum Nutr 2010; 65(2):170-8. https://doi.org/10.1007/s11130-010-0168-2

Adedapo AA, Jimoh FO, Koduru S, Masika PJ, Afolayan AJ. Evaluation of the medicinal potentials of the methanol extracts of the leaves and stems of Halleria lucida. Bioresour Technol 2008; 99(10):4158-63. DOI: 10.1016/j.biortech.2007.08.066.https://doi.org/10.1016/j.biortech.2007.08.066

Gordon MH. The mechanism of antioxidant action in vitro. In Food antioxidants 1990 (pp. 1-18). Springer, Dordrecht.https://doi.org/10.1007/978-94-009-0753-9_1

Lu Y, Foo LY. Antioxidant activities of polyphenols from sage (Salvia officinalis). Food Chem 2001; 75(2):197-202. DOI: 10.1016/s0308-8146(01)00198-4.https://doi.org/10.1016/S0308-8146(01)00198-4

Obistioiu D, Cristina RT, Schmerold I, Chizzola R, Stolze K, Nichita I, Chiurciu V. Chemical characterization by GC-MS and in vitro activity against Candida albicans of volatile fractions prepared from Artemisia dracunculus, Artemisia abrotanum, Artemisia absinthium and Artemisia vulgaris. Chem Cent J 2014; 8(1):6. https://doi.org/10.1186/1752-153X-8-6

Sun H, Zhang F, Chen S, Guan Z, Jiang J, Fang W, Chen F. Effects of aphid herbivory on volatile organic compounds of Artemisia annua and Chrysanthemum morifolium. Biochem Syst Ecol 2015; 60:225-33. DOI: 10.1016/j.bse.2015.04.023.https://doi.org/10.1016/j.bse.2015.04.023

Wang Y, Li X, Jiang Q, Sun H, Jiang J, Chen S, Guan Z, Fang W, Chen F. GC-MS Analysis of the volatile constituents in the leaves of 14 Compositae plants. Molecules 2018; 23(1):166. DOI: 10.3390/molecules23010166.https://doi.org/10.3390/molecules23010166

Zhao F, Wang P, Lucardi RD, Su Z, Li S. Natural sources and bioactivities of 2, 4-di-tert-butylphenol and its analogs. Toxins 2020; 12(1):35. DOI: 10.3390/toxins12010035.https://doi.org/10.3390/toxins12010035

Seol GH, Kim KY. Eucalyptol and its role in chronic diseases. Drug Discovery from Mother Nature. 2016: 389-98. DOI: 10.1007/978-3-319-41342-6_18.https://doi.org/10.1007/978-3-319-41342-6_18

Rajkumar S, Jebanesan A. Mosquitocidal activities of octacosane from Moschosma polystachyum Linn. (Lamiaceae). J Ethnopharmacol 2004; 90(1): 87-9. DOI: 10.1016/j.jep.2003.09.030.https://doi.org/10.1016/j.jep.2003.09.030

Published

07-03-2022 — Updated on 01-04-2022

Versions

How to Cite

1.
Mayuri M, Asima D, Joseph KS. Phytochemical analysis and antioxidant activities of Artemisia stelleriana Besser leaf extracts. Plant Sci. Today [Internet]. 2022 Apr. 1 [cited 2024 Mar. 29];9(2):215-20. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/1263

Issue

Section

Research communications