Evaluation of growth pattern and biochemical components of Chlamydomonas reinhardtii Dangeard

Authors

DOI:

https://doi.org/10.14719/pst.1308

Keywords:

Algae, Chlamydomonas reinhardtii, Lipids, Pigment system, Proteins

Abstract

Chlamydomonas reinhardtii Dangeard is the simplest motile, unicellular fresh water alga of class Chlorophyceae. It also functions as the most efficient model system for converting solar energy to chemical energy in the form of various metabolites. The objective of the present work is to deal with the growth conditions/growth kinetics of Chlamydomonas reinhardtii Dangeard, required for optimal biomass production. The parameter used to study growth of algae was, photosynthetic measurement, biomass, proteins and lipid measurement, which vary with the change in the cultural conditions. Present investigations reveal that change in protein content is positively correlated with the increase in biomass, revealing that the algae can grow rapidly in laboratory/cultural conditions. Lipid content shows a negative correlation with proteins and biomass. Lipids are known to have a role as structural components, in hydration and also in signaling events. Lipids, mainly the triacyl glycerides (TAGs) act as storage compounds enabling the microalgae to survive in adverse environmental conditions. Lipidic content increases in Chlamydomonas reinhardtii increases with optimal light and nutrient system. The increase is in the form of triacyl glycerides which serve as precursors for the production of biodiesel and bioethanol. Conclusion- Further research is required to investigate the interactions of biomolecules and growth of algae.

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References

Fritsch FE. The structure and reproduction of Algae. Vol I, Cambridge University Press. Cambridge, UK. 1935.

Smith GM. Cryptogamic Botany, Vol. I, McGraw-Hill Co., New York. 1955.

Dangeard PA. Recherches sur les algues inférieures. Annales des Sciences Naturelles. Botanique, série.1888;7:105-75.

Dubini A. Biofuel production from Chlamydomonas reinhardtii, Green energy. Biochem Soc. 2011;20-34. https://doi.org/10.1042/BIO03302020

Coimbra S, Almeida J, Junqueira V, Cosa ML, Pereira LG. Arabinogalactan proteins as molecular marker in Arabidopsis thaliana sexual reproduction. J Exp Bot. 2007; 58: 4027-35. https://doi.org/10.1093/jxb/erm259

Ellis M, Egelund J, Schultz CJ, Bacic A. Arabinogalactan- proteins: key regulators at the cell surface? Plant Physiol. 2010;153:403-19. https://doi.org/10.1104/pp.110.156000

Losada JM, Herrero M. Arabinogalactan-protein secretion is associated with the acquisition of stigmatic receptivity in the apple flower. Ann Bot. 2012; 110: 573-84. https://doi.org/10.1093/aob/mcs116

Hiscock SJ, Allen AM. Diverse signalling pathways regulate pollen-stigma interactions: the search for consensus. New Phytol. 2008; 179: 286-317. https://doi.org/10.1111/j.1469-8137.2008.02457.x

Sang Y, Xu M, Ma F, Xu X, Gao X-Q, Zhang XS. Comparative proteomic analysis reveals similar and distinct features of proteins in dry and wet stigmas. Proteomics. 2012; 12: 1983-98. https://doi.org/10.1002/pmic.201100407

Work VH, Radakovits R, Jinkerson RE, Meuser JE, Elliott LG, Vinyard, DJ, Laurens LML, Dismukes CJ, Posewitz. Increased lipid accumulation in the Chlamydomonas reinhardtiista 7-10 starchless isoamylase mutant and increased carbohydrate synthesis in complemented strains. Eukaryotic Cell. 2010; 9: 1251-61.https://doi.org/10.1128/EC.00075-10

Giroud C, Gerber A, Eichenberger W. Lipids of Chlamydomonas reinhardtii. Analysis of molecular species and intracellular sites(s) of biosynthesis. Plant Cell Physiol. 1988; 29: 587-95.

Soeder CJ, Bloze A. Sulphate deficiency stimulates release of dissolved organic matter in synchronous cultures of Scenedesmus obliquus. Physiol. Plat.1981; 52: 233-38. https://doi.org/10.1111/j.1399-3054.1981.tb08498.x

Ratha SK, Rao PH, Govindaswamy K, Jaswin RS, Lakshmidevi R. et al. A rapid and reliable method for estimating microalgal biomass using a moisture analyser. J Appl Phycol. 2016;28:1725-34 https://doi.org/10.1007/s10811-015-0731-1

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin Phenol reagent. J Biol Chem. 1951;193:265-75. https://doi.org/10.1016/S0021-9258(19)52451-6

Bligh EG, Dyer WJ. A rapid method for total lipid extraction and purification. Can J Biochem Physiol. 1959;37:911-17. https://doi.org/10.1139/o59-099

Lichtenthale HK, Buschmann C. Chlorophylls and carotenoids: Measurement and characterization by UV-VIS spectroscopy. Curr Protoc Food Anal Chem. 2001; F 4.3.1-F 4.3.8. https://doi.org/10.1002/0471142913.faf0403s01

Harris EH. The Chlamydomonas sourcebook. A Comprehensive Guide to Biology and Laboratory Use. New York: Academic Press, San Diego, CA. 1989.

Roberts K. Crystalline glycoprotein cell walls of algae: their structure, composition and assemblely. Phil. Trans. R. Soc. Lond.(B) 1974; 268: 129-146. https://doi.org/10.1098/rstb.1974.0021

Fischer BB, Wiesendanger M, Eggen RI.L. Growth condition-dependent sensitivity, photodamage and stress response of Chlamydomonas reinhardtii exposed to high light conditions. Plant Cell Physiol. 2006; 47: 1135-45 https://doi.org/10.1093/pcp/pcj085

Bishop CL. Life cycle control of Chlamydomonas reinhardtii, Genome Biol. 2003; 4: 20030423-01 https://doi.org/10.1186/gb-spotlight-20030423-01

Tamburic B, Zemichael F, Maitland G, Hellgardt K. Parameters affecting the growth and hydrogen production of the green alga Chlamydomonas reinhardtii. Int J Hydrog Energy. 2011;36:7872-76. https://doi.org/10.1016/j.ijhydene.2010.11.074

Juergens M, Deshpande R, Lucker B, Park J, Wan H, Gargouri M, Holguin FO, Disbrow B, Tanner S, Skepper J, Kramer D, Gang Hicks L, Shachar-Hill Y. The regulation of photosynthetic structure and function during nitrogen deprivation in Chlamydomonas reinhardtii. Plant Physiol. 2015;167:558-73.https://doi.org/10.1104/pp.114.250530

Xiaodong D, Jiajia C, Yajun L, Xiaowen F. Expression and knockdown of the PEPC1 gene affect carbon flux in the biosynthesis of triacylglycerols by the green alga Chlamydomonas reinhardtii. Biotechnol Lett. 2014; 36:2199-2208 https://doi.org/10.1007/s10529-014-1593-3

Miller R, Wu G, Deshpande RR, Vieler A, Gärtner K, Li X, Moellering ER, Zäuner S, Cornish AJ, Liu B, Bullard B, Sears B, Kuo MVEL, Shachar-Hill Y, Shiu S, Benning C. Changes in transcript abundance in Chlamydomonas reinhardtii following nitrogen deprivation predict diversion of metabolism. Plant Physiol. 2010; 154:1737-52 https://doi.org/10.1104/pp.110.165159

Siaut M, Cuine S, Cagnon C, Fesssler B, Nguyen M, Carrier P, Beyly A, Beisson F, Triantaphylides C, Beisson Y, Peltier G. Oil accumulation in the model green alga Chlamydomonas reinhardtii: characterization, variability between common laboratory strains and relationship with starch reserves. Biotechnology. 2011;11:7. https://doi.org/10.1186/1472-6750-11-7

Soto-Sierra L, Wilken LR, Dixon CK. Aqueous enzymatic protein and lipid release from the microalgae Chlamydomonas reinhardtii. Bioresour. Bioprocess.2020; 7, 46 https://doi.org/10.1186/s40643-020-00328-4

Sharma, KK, Schuhmann H, Schenk PM. High lipid induction in microalgae for biodiesel production. Energies. 2012; 5: 1532-15553. https://doi.org/10.3390/en5051532

Kim EJ, Kim S, Choi HG. et al. Co-production of biodiesel and bioethanol using psychrophilic microalga Chlamydomonas sp. KNM0029C isolated from Arctic sea ice. Biotechnol Biofuels 2020; 13, 20. https://doi.org/10.1186/s13068-020-1660-z

Published

01-02-2022 — Updated on 01-04-2022

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How to Cite

1.
Sharma B. Evaluation of growth pattern and biochemical components of Chlamydomonas reinhardtii Dangeard . Plant Sci. Today [Internet]. 2022 Apr. 1 [cited 2024 Mar. 29];9(2):221-7. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/1308

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Research Articles