In vitro growth and content of vincristine and vinblastine of Catharanthus roseus L. hairy roots in response to precursors and elicitors
DOI:
https://doi.org/10.14719/pst.1337Keywords:
Hairy root, ElicitorAbstract
Catharanthus roseus L. is a medicinal plant that produces numerous indole terpenoid alkaloids, including vincristine and vinblastine, which are used for cancer treatment. The effect of specified precursors (L-phenylalanine, L-tyrosine) and elicitors (chitosan, methyl jasmonate) on C. roseus hairy roots (CHR) growth has been examined in order to increase the content of vincristine and vinblastine. Our results showed that CHR generated by an Agrobacterium rhizogenes strain isolated in Vietnam was capable of producing both vincristine and vinblastine when subjected to precursors, but only vinblastine when exposed to elicitors. However, both precursors and elicitors were evaluated to have an effect on increasing the accumulation of TIAs in CHR. In particular, the use of elicitors required more time to find the appropriate induction conditions, while the use of precursors gave outstanding efficiency in the treatment with 1 µM phenylalanine. The greatest yields of vincristine (51.99 µg g-1 DW) and vinblastine (699.92 µg g-1 DW) were obtained in the 7th week (with 0.306 g DW biomass). This result is the first time we might boost the levels of vincristine and vinblastine in our CHR clone generated by the Vietnam strain of A. rhizogenes.
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Gajalakshmi S, Vijayalakshmi S, Devi RV. Pharmacological activities of Catharanthus roseus: A perspective review [Internet]. Psu.edu. [cited 2021 Dec 1]. Available from: http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.302.1823&rep=rep1&type=pdf
Das S, Sharangi AB. Madagascar periwinkle (Catharanthus roseus L.): Diverse medicinal and therapeutic benefits to humankind. J Pharmacogn Phytochem. 2017;6(5):1695–701. Available from: https://www.phytojournal.com/archives/2017/vol6issue5/PartY/6-5-182-590.pdf.
Aslam J, Khan SH, Siddiqui ZH, Fatima Z, Maqsood M, Bhat MA, Nasim SA, Ilah A, Ahmad IZ, Khan SA, Mujib A. Catharanthus roseus (L.) G. Don. An important drug: it’s applications and production. Pharmacie Globale (IJCP). 2010;4(12):1-6.
Kabesh K, Senthilkumar P, Ragunathan R, Kumar RR. Phytochemical analysis of Catharanthus roseus plant extract and its antimicrobial activity. Int J Pure App Biosci. 2015;3(2):162-72. Available from: http://www.ijpab.com/form/2015%20Volume%203,%20issue%202/IJPAB-2015-3-2-162-172.pdf
Alam MM, Naeem M, Khan MMA, Uddin M. Vincristine and vinblastine anticancer Catharanthus alkaloids: Pharmacological Applications and Strategies for Yield Improvement. In: Naeem M., Aftab T., Khan M. (eds) Catharanthus roseus. Springer, Cham. 2017. https://doi.org/10.1007/978-3-319-51620-2_11.
Van Der Heijden R, Jacobs DI, Snoeijer W, Hallard D, Verpoorte R. The Catharanthus alkaloids: pharmacognosy and biotechnology. Curr Med Chem. 2004;11(5):607–28. https://doi.org/10.2174/0929867043455846
Sottomayor M, Barceló AR. The Vinca alkaloids: From biosynthesis and accumulation in plant cells, to uptake, activity and metabolism in animal cells. In: Studies in Natural Products Chemistry. Elsevier; 2006. p. 813–57. https://doi.org/10.1016/S1572-5995(06)80041-4
Yu F, De Luca V. ATP-binding cassette transporter controls leaf surface secretion of anticancer drug components in Catharanthus roseus. Proc Natl Acad Sci U S A. 2013;110(39):15830–5. https://doi.org/10.1073/pnas.1307504110
Parihar S, Dwivedi NK. Comprehensive analysis of liquid and semisolid culture system for in vitro propagation and conservation of Caralluma edulis: an appetite suppressant medicinal succulent of the indian thar desert. Plant Cell Biotechnol Mol Biol. 2019 Nov 23:1020-31. Available from: https://www.ikppress.org/index.php/PCBMB/article/view/4799
Shanks JV, Morgan J. Plant “hairy root” culture. Curr Opin Biotechnol. 1999;10(2):151–15. https://doi.org/10.1016/S0958-1669(99)80026-3
Wang JW, Wu JY. Effective elicitors and process strategies for enhancement of secondary metabolite production in hairy root cultures. Adv Biochem Eng Biotechnol. 2013;134:55–89. https://doi.org/10.1007/10_2013_183
Ruiz-May E, Galaz-Avalos RM, Loyola-Vargas VM. Differential secretion and accumulation of terpene indole alkaloids in hairy roots of Catharanthus roseus treated with methyl jasmonate. Mol Biotechnol. 2009;41(3):278–85. https://doi.org/10.1007/s12033-008-9111-2
Zhou M-L, Zhu X-M, Shao J-R, Wu Y-M, Tang Y-X. Transcriptional response of the catharanthine biosynthesis pathway to methyl jasmonate/nitric oxide elicitation in Catharanthus roseus hairy root culture. Appl Microbiol Biotechnol. 2010;88(3):737–50. https://doi.org/10.1007/s00253-010-2822-x
Rijhwani SK, Shanks JV. Effect of elicitor dosage and exposure time on biosynthesis of indole alkaloids by Catharanthus roseus hairy root cultures. Biotechnol Prog. 1998;14(3):442–49. https://doi.org/10.1021/bp980029v
Nguyen NN, Van Bui L. Isolation and identification of Agrobacterium rhizogenes inducing hairy roots of Catharanthus roseus in vivo. Sci Technol Dev J - Nat Sci. 2017;1(T1):48–59. https://doi.org/10.32508/stdjns.v1iT1.434
Nguyen NN, Le BV. Hairy root induction in Catharanthus roseus by various strains of Agrobacterium rhizogenes isolated in Vietnam. Science and Technology Development Journal. 2016 Jun 30;19(2):44-52. https://doi.org/10.32508/stdj.v19i2.788
Gamborg OL, Miller RA, Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968;50(1):151–58. https://doi.org/10.1016/0014-4827(68)90403-5
Zhou H, Tai Y, Sun C, Pan Y. Rapid identification of vinca alkaloids by direct-injection electrospray ionisation tandem mass spectrometry and confirmation by high-performance liquid chromatography-mass spectrometry. Phytochem Anal. 2005;16(5):328–33. https://doi.org/10.1002/pca.852
Alam P, Sharaf-Eldin MA. Limited production of plant derived anticancer drugs vinblastine and vincristine. Planta Med [Internet]. 2016;82(05). https://doi.org/10.1055/s-0036-1578706
Cao DM, Vu PTB, Hoang MTT, Bui AL, Quach PND. Developing a sufficient protocol for the enhancement of ?-glucosidase inhibitory activity by Urena lobata L. aeroponic hairy roots using exogenous factors, a precursor and an elicitor. Plants. 2020;9(4):548. https://doi.org/10.3390/plants9040548
Shinde AN, Malpathak N, Fulzele DP. Enhanced production of phytoestrogenic isoflavones from hairy root cultures of Psoralea corylifolia L. Using elicitation and precursor feeding. Biotechnol Bioprocess Eng. 2009;14(3):288–94. https://doi.org/10.1007/s12257-008-0238-6
Kutchan TM. Alkaloid biosynthesis - The basis for metabolic engineering of medicinal plants. Plant Cell. 1995;7(7):1059–70. https://doi.org/10.1105/tpc.7.7.1059
Peebles CAM, Hong S-B, Gibson SI, Shanks JV, San K-Y. Effects of terpenoid precursor feeding on Catharanthus roseus hairy roots over-expressing the alpha or the alpha and beta subunits of anthranilate synthase. Biotechnol Bioeng. 2006;93(3):534–40. https://doi.org/10.1002/bit.20739
Gaviraj EN, Veeresham C. Effect of precursors and organic compounds on alkaloid production in transformed root cultures of Catharanthus roseus. var. nirmal. Pharm Biol. 2006;44(5):371–7. https://doi.org/10.1080/13880200600748929
Zhou P, Yang J, Zhu J, He S, Zhang W, Yu R, Zi J, Song L, Huang X. Effects of ?-cyclodextrin and methyl jasmonate on the production of vindoline, catharanthine and ajmalicine in Catharanthus roseus cambial meristematic cell cultures. Appl Microbiol Biotechnol. 2015;99(17):7035–45. https://doi.org/10.1007/s00253-015-6651-9
Almagro L, López Perez AJ, Pedreño MA. New method to enhance ajmalicine production in Catharanthus roseus cell cultures based on the use of cyclodextrins. Biotechnol Lett. 2011;33(2):381–85. https://doi.org/10.1007/s10529-010-0430-6
Vázquez-Flota F, Moreno-Valenzuela O, Miranda-Ham ML, Coello-Coello J, Loyola-Vargas VM. Catharanthine and ajmalicine synthesis in Catharanthus roseus hairy root cultures: Medium optimization and elicitation. In: Primary and Secondary Metabolism of Plants and Cell Cultures III. Dordrecht: Springer Netherlands; 1994. p. 273–79. https://doi.org/10.1007/978-94-011-0237-7_23
Pan Q, Saiman MZ, Verpoorte R, Tang K. Accumulation of terpenoid indole alkaloids in jasmonic acid elicited Catharanthus roseus plants before and during flowering. Pak J Bot. 2018;50(3):1077-83. Available from: http://www.pakbs.org/pjbot/papers/1521355666.pdf
Hanafy MS, Matter MA, Asker MS, Rady MR. Production of indole alkaloids in hairy root cultures of Catharanthus roseus L. and their antimicrobial activity. S Afr J Bot. 2016;105:9–18. https://doi.org/10.1016/j.sajb.2016.01.004
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