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Plant Science Today (PST)

Research Articles

Vol. 8 No. 4 (2021)

Effect of Agrobacterium co-cultivation stage on explant response for subsequent genetic transformation in Soybean (Glycine max (L.) Merr.)

DOI
https://doi.org/10.14719/pst.2021.8.4.1363
Submitted
2 July 2021
Published
30-09-2021 — Updated on 01-10-2021

Abstract

The establishment of an efficient in vitro genetic transformation protocol in soybean depends upon an effective interaction between the explants and Agrobacterium tumefaciens. Therefore, a study was conducted at the University of Limpopo, South Africa, between September 2019 and May 2020 to evaluate explant amenability and effects of Agrobacterium co-cultivation stage on the induction of oxidative stress. This stress potentially causes lipid peroxidation, reduction of phytochemicals and chlorophyll pigments on explant tissue targeted for genetic transformation. This study, used double cotyledonary node explants infected and co-cultured with A. tumefaciens to evaluate total phenolics, antioxidant activity, lipid peroxidation and oxidative stress-induced tissue senescence during the co-cultivation stage. The results, showed that, explant co-cultivation with Agrobacterium for 2, 4 and 6 days caused reductions in the amounts of phenolic compounds, chlorophylls and antioxidant activity due to tissue senescence, high oxidative stress and malondialdehyde contents. Percentage phenolic content of all bacteria infected explants ranged between 10.3?10.6 compared to 20.9% in the control. Chlorophyll content of about 1.49?4.00 mg/ml and malondialdehyde content ranging between 1.0?5.7 mM-1g-1 were also recorded. Overall, findings suggest that the infection of explants with A. tumefaciens can induce oxidative stress and tissue senescence depending on the period of co-cultivation. However, reduced oxidative stress and senescence of explant tissues may potentially improve soybean shoot regeneration and transformation efficiency.

References

  1. Mangena P. Genetic transformation to confer drought stress tolerance in Soybean (Glycine max L.). In: Guleria P, Kumar V, Lichtfouse E (eds) Sustainable Agriculture Reviews 45. Sustainable Agriculture Review. 2020; vol 45. Springer, Cham. https://doi.org/10.1007/978-3-030-53017-4_10
  2. Li S, Y Cong, Y Liu, T Wang, Q Shuai, N Chen, J Gai J, Y Li. Optimization of Agrobacterium-mediated transformation in soybean. Front Plant Sci. 2017;8(246):1?15. https://doi.org/10.3389/fpls.2017.00246
  3. Mangena P, EKM Sehaole. Transgenic grain legumes. In: Mangena P (eds) Advances in Legume Research- Physiological Responses and Genetic Improvement for Stress Resistance. Bentham Science Publishers, Singapore. 2020; vol 1, pp. 148?72. https://doi.org/10.2174/9789811479625120010011
  4. Hsieh C, S Fernandez-Tome, B Hernandez-Ledesma. Functionality of soybean compounds in the oxidative stress-related disorders. In: Gracia-Sancho J, Salvado J (eds) Gastrointestinal Tissue: Oxidative Stress and Dietary Antioxidant. Academic Press. 2017; pp. 339?53. https://doi.org/10.1016/B978-0-12-805377-5.00027-8
  5. Dan Y. Biological functions of antioxidants in plant transformation. In Vitro Cell Dev Biol Plant. 2008;44(3):149?61. https://doi.org/10.1007/s11627-008-9110-9
  6. Paz MM, JC Martinez, AB Kalvig, TM Fonger, K Wang. Improved cotyledonary node method using an alternative explant derived from mature seed or efficient Agrobacterium-mediated soybean transformation. Plant Cell Rep. 2006;25:206?13. https://doi.org/10.1007/s00299-005-0048-7
  7. Trigiano RN, DJ Gray. Plant development and biotechnology. CRC Press, Washington, D.C., 2005; pp. 9?37.
  8. Baskaran P, M Moyo, J van Staden. In vitro plant regeneration, phenolic compound production and pharmacological activities of Coleonema pulchellum. S Afr J Bot. 2014;90:74?79. https://doi.org/10.1016/j.sajb.2013.10.005
  9. Nikolova M, M Petrova, E Zayoza, A Vitkova, L Evstatieva. Comparative study of in vitro, ex vitro and in vivo grown plants of Arnica montana-polyphenols and free radical scavenging activity. Acta Bot Croat. 2013;72(1):13?22. https://doi.org/10.2478/v10184-012-00139
  10. Sharma OP, TK Bhat. DPPH antioxidant assay revisited. Food Chem. 2009;113:1202?05. https://doi.org/10.1016/j.foodchem.2008.08.008
  11. Jimoh MO, AJ Afolayan, FB Lewu. Antioxidant and phytochemical activities of Amaranthus caudatus L. harvested from different soils at various growth stages. Sci Rep. 2019;9: 12965. https://doi.org/10.1038/s41598-019-49276
  12. Boyer RF. Isolation and spectrophotometric characterization of photosynthetic pigments. Biochem Edu. 1990;18(4):203?06.
  13. Ali MB, EJ Hahn, KY Paek. Effects of light intensities on antioxidant enzymes and malondialdehyde content during short-term acclimatization on micropropagated Phalaenopsis plantlet. Environ Exp Bot. 2005;54:109?20. https://doi.org/10.1016/j.envexpbot.2004.06.005
  14. Zhang Z, JJ Finer. Low Agrobacterium tumefaciens inoculum levels and a long co-culture period lead to reduced plant defense responses and increase transgenic shoot production of sunflower (Helianthus annuus L.). In Vitro Cell and Dev Biol- Plant. 2016;52:354?66. https://doi.org/10.1007/s11627-016-9774-5
  15. Zia M, W Arshad, Y Bibi, S Nisa, MF Chaudhary. Does Agro-injection to soybean pods transform embryos? Plant Omics J. 2011;4(7):384?90. https://pomics.com/zia_4_7_2011_384_390.pdf
  16. Jain R, SK Jain. Total phenolic contents and antioxidant activities of some selected anti-cancer medicinal plants from Chhattisgarh State, India. Pharmacologyonline. 2011;2:755?62.
  17. Golkar P, A Arzani, AM Rezaei, Z Yarali, M Yousefi. Genetic variation of leaf antioxidants and chlorophyll content in safflower. Afr J Agric Res. 2010;4(12):1475?82.
  18. Adams ZP, J Ehlting, R Edwards. The regulatory role of shikimate in plant phenylalanine metabolism. J Theor Biol. 2019;462:158?570. https://doi.org/10.1016/j.jtbi.2018.11.005
  19. Choi DG, NH Yoo, CYY Yu, BL Reyes, SJ Yun. The activities of antioxidant enzymes in response to oxidative stresses and hormones in paraquat-tolerant Rehmannia glutinosa plants. J Biochem Mol Biol. 2004;37(5):618?24. https://www.doi.org/10.5483/bmbrep.2004.37.5.618
  20. Paz MM, JC Martinez, AB Kalvig, TM Fonger, K Wang. Assessment of conditions affecting Agrobacterium-mediated soybean transformation using the cotyledonary node explant. Euphytica. 2004;136:167?79. https://doi.org/10.1023/B:EUPH.0000030669.75809.dc
  21. Hiei Y, S Ohta, T Komari, T Kumashiro. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J. 1994;6:271?82.

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