Anti-bacterial, Anti-oxidant and other Phytochemical Properties of Datura innoxia leaves
DOI:
https://doi.org/10.14719/pst.1387Keywords:
Datura innoxia, IC 50, Kirby-Bauer test, Phytochemical screening, Antioxidant activity.Abstract
To investigate the phytochemicals present in the leaves of Datura innoxia and to assess its antioxidant and antibacterial properties in different organic solvents, leaf extracts were exposed to different standardized techniques. Folin–Ciocalteu method and Aluminium chloride method proved that the ethanolic extract has maximum phenolic content (72.35± 0.52 mg GAE/g) and flavonoid content (29.21± 1.25 mg EQ/g) respectively. The highest DPPH radical scavenging activity with IC50 value 91.398 µg/ml also was in the ethanolic extract as compared to methanol, hexane and chloroform extracts. Free radical scavenging and antioxidant property of extracts were observed in the sequence of ethanol>methanol>hexane>chloroform. There was a strong correlation between antioxidant activity with total phenolic (DPPH, R2 = 0.41; PPM, R2 = 0.25) and total flavonoid contents (DPPH, R2 = 0.39; PPM, R2=0.23). Ethanolic and methanolic extracts showed antibacterial potential against the tested pathogenic strains; Staphylococcus aureus and Escherichia coli with a zone of inhibition ranging between 16± 0.9 to 27.5±0.8 mm. This study has proved that ethanolic leaf extract of D. innoxia showed bacterial inhibition and antioxidant activities and this herb can be assessed as a potential therapeutic species.
Downloads
References
Fadl Almoulah N, Voynikov Y, Gevrenova R et al. Antibacterial, antiproliferative and antioxidant activity of leaf extracts of selected Solanaceae species. South African J Bot. 2017;112:368–74. https://doi.org/10.1016/j.sajb.2017.06.016
Afroz M, Akter S, Ahmed A et al. Ethnobotany and antimicrobial peptides from plants of the Solanaceae family: An update and future prospects. Front Pharmacol. 2020;11. https://doi.org/10.3389/fphar.2020.00565
Shah VV, Shah ND, Patrekar PV. Medicinal plants from the Solanaceae family. Res J Pharm Technol. 2013;6:143–51.
Ralte L, Bhardwaj U, Singh YT. Traditionally used edible Solanaceae plants of Mizoram, India have high antioxidant and antimicrobial potential for effective phytopharmaceutical and nutraceutical formulations. Heliyon. 2021. https://doi.org/10.1016/j.heliyon.2021.e07907
Kaushik P, Goyal P. In vitro evaluation of Datura innoxia (thorn-apple) for potential antibacterial activity. Indian J Microbiol. 2008;48:353–57. https://doi.org/10.1007/s12088-008-0020-7
Papoutsis I, Nikolaou P, Athanaselis S et al. Mass intoxication with Datura innoxia-case series and confirmation by analytical toxicology. Clin Toxicol. 2010;48:143–45. https://doi.org/10.3109/15563650903524134
Henderson L. Alien weeds and invasive plants. A complete guide to declared weeds and invaders in South Africa. Plant Protection Research Institute Handbook No. 12. PPR, ARC South Africa. 2001; P. 300.
Sharma M, Dhaliwal I, Rana K et al. Phytochemistry, pharmacology and toxicology of Datura species-a review. Antioxidants. 2021;10:1–12. https://doi.org/10.3390/antiox10081291
Li Q, Zhu T, Zhang R et al. Molecular cloning and functional analysis of hyoscyamine 6?-hydroxylase (H6H) in the poisonous and medicinal plant Datura innoxia Mill. Plant Physiol Biochem. 2020;153:11–19. https://doi.org/10.1016/J.PLAPHY.2020.04.021
Fatima H, Khan K, Zia M et al. Extraction optimization of medicinally important metabolites from Datura innoxia Mill.: An in vitro biological and phytochemical investigation. BMC Complement Altern Med. 2015 (15). https://doi.org/10.1186/s12906-015-0891-1
Neeraj O. Maheshwari, Ayesha Khan BAC. Rediscovering the medicinal properties of Datura sp.: A review. J Med Plants Re.2013;7:2885–97. https://doi.org/10.5897/JMPR11.1657
CABI. Invasive Species Compendium. Wallingford, UK: CAB International. (Accessed February 2021).www.cabi.org/inc.
Doshi KA, Patel MH, Bhoomi K. “Dhatura (Datura innoxia Mill.)” a precious toxic plant - a review. Int J Ayurvedic Herb Med. 2015;5:1785–1803.
Jalander V, Gachande BD. Effect of aqueous leaf extracts of Datura Sp. against two plant pathogenic fungi. Online) An Online Int J Available. 2012; 2:131–34.
Gajendran B, Durai P, Varier KM et al. Green synthesis of silver nanoparticle from Datura inoxia flower extract and its cytotoxic activity. Bionanoscience. 2019; 9:564–72. https://doi.org/10.1007/s12668-019-00645-9.
Kosova V, Chladek M. A pharma-cognostic study of Datura metel and D. innoxia. Pharmazie.1957;12:620.
Baig MW, Nasir B, Waseem D et al. Withametelin: a biologically active withanolide in cancer, inflammation, pain and depression. Saudi Pharm J. 2020; 28:1526–37. https://doi.org/10.1016/j.jsps.2020.09.021
Dafni A, Yaniv Z. Solanaceae as medicinal plants in Israel. J Ethnopharmacol. 1994. https://doi.org/10.1016/0378-8741(94)90093-0
Mousavi-Khattat M, Keyhanfar M, Razmjou A. A comparative study of stability, antioxidant, DNA cleavage and antibacterial activities of green and chemically synthesized silver nanoparticles. Artif Cells, Nanomedicine Biotechnol. 2018; 46:S1022–S1031. https://doi.org/10.1080/21691401.2018.1527346
Chandni T, Priti M, Manodeep S. Antibacterial compounds in different species of Datura: A review. African J Microbiol Res. 2014;8:2090–98. https://doi.org/10.5897/ajmr2014.6805
Céspede, Méndez C, Iturriaga-Vásquez P, Hormazábal E. Secondary metabolites and biological profiles of Datura genus. J Chil Chem Soc. 2021;66:51–83. https://doi.org/10.4067/S0717-97072021000205183
Vermillion K, Holguin FO, Berhow MA et al. Dinoxin B, a withanolide from Datura inoxia leaves with specific cytotoxic activities. J Nat Prod. 2011;74:267–71. https://doi.org/10.1021/np1004714
Vaillant N, Monnet F, Hitmi A et al. Comparative study of responses in four Datura species to a zinc stress. Chemosphere. 2005;59:1005–13. https://doi.org/10.1016/j.chemosphere.2004.11.030
Singh A, Duggal S, Singh H et al. Withanolides: Phytoconstituents with significant pharmacological activities. Int J Green Pharm. 2010; 4:229–37. https://doi.org/10.4103/0973-8258.74130
Ahlawat S, Saxena P, Ali A et al. Comparative study of withanolide production and the related transcriptional responses of biosynthetic genes in fungi elicited cell suspension culture of Withania somnifera in shake flask and bioreactor. Plant Physiol Biochem. 2017; 114:19–28. https://doi.org/10.1016/j.plaphy.2017.02.013
Mirjalili MH, Moyano E, Bonfill M et al. Steroidal lactones from Withania somnifera, an ancient plant for novel medicine. Molecules. 2009;14:2373–93. https://doi.org/10.3390/molecules14072373
Tandon C, Mathur P, Sen M, Kanojiya S. Identification of an antibacterial withanolide (Dinoxin b) from leaf of Datura innoxia Mill. Int J Phytomedicine. 2016;8:1–12.
S. Deshmukh, Shelke PD, Palekar KS, Pawar SD, HS Shinde. Antimicrobial investigation of Datura stramonium leaf extract against different microorganisms. IOSR J Environ Sci. 2015;9(9):17-19. doi:10.9790/2402-09911719
Trease GE, Evans WC. Textbook of Pharmacognosy. 12th Edition (Balliere, Tindall, London. 1983; p 57-59.
1Harborne, J.B. Phytochemical Methods. Chapman and Hall Ltd., London,1973;p 49-188.
Datta N, Pal M, Roy U et al. World Journal of Pharmaceutical Research Infection. 2014; 13:15
Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. American Journal of Enology and Viticulture. 1965;16:144–58.
Dao BA, Togola I, Daou C. Preparation technique of green tea (Camellia sinensis) in Grins in Mali: Phytochemical profiles and antioxidant activity. J Food Stud. 2021;10:26. https://doi.org/10.5296/jfs.v10i1.18701
Ordon-Ez AA, Gomez JD, Vattuone MA, Isla MI. Antioxidant activities of Sechium edule (Jacq.) Swart extracts. Food Chem. 2006;97:452-58. https://doi.org/10.1016/j.foodchem.2005.05.024
Benabderrahim MA, Sarikurkcu C, Elfalleh W, Ozer MS. Datura innoxia and Dipsacus laciniatus: Biological activity and phenolic composition. Biocatal Agric Biotechnol. 2019; 19:101163. https://doi.org/10.1016/j.bcab.2019.101163
Togola I, Dembélé AA, Tounkara F et al. Evaluation of in vitro antioxidant activities of ethanol extracts of Datura innoxia Mill. leaves and seeds harvested in Mali. Annu Res Rev Biol. 2019;33:1–8. https://doi.org/10.9734/arrb/2019/v33i230115
Bellila A, Tremblay C, Pichette A et al. Cytotoxic activity of withanolides isolated from Tunisian Datura metel L. Phytochemistry. 2011. https://doi.org/10.1016/j.phytochem.2011.07.009
Jan S, Khan MR, Rashid U, Bokhari J. Assessment of antioxidant potential, total phenolics and flavonoids of different solvent fractions of Monotheca buxifolia fruit. Osong Public Heal Res Perspect. 2013;4:246–54. https://doi.org/10.1016/j.phrp.2013.09.003
Hudzicki J . Kirby-Bauer Disk Diffusion susceptibility test protocol author information. Am Soc Microbiol. 2012;1–13.
Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal. 2016;6:71–79. https://doi.org/10.1016/j.jpha.2015.11.005
Baker CN, Thornsberry C, Hawkinson RW. Inoculum standardization in antimicrobial susceptibility tests: evaluation of the overnight agar cultures and the rapid inoculum standardization system. J Clin Micro. 1983;17:450-57. https://doi.org/10.1128/jcm.17.3.450-457.1983
Barry AL, Coyle MB, Thornsberry C et al. Methods of measuring zones of inhibition with the Bauer-Kirby disk susceptibility test. J Clin Microbiol. 1979. https://doi.org/10.1128/jcm.10.6.885-889.1979
Ayuba VO, Ojobe TO, Ayuba SA. Datura phytochemical. J Med Plants Research. 2011.
Sharma M, Dhaliwal I, Rana K et al. Phytochemistry, pharmacology and toxicology of Datura species—a review. Antioxidants. 2021;10:1–12. https://doi.org/10.3390/antiox10081291
Sharma DC, Shukla R, Ali J et al. Phytochemical evaluation, antioxidant assay, antibacterial activity and determination of cell viability (J774 and THP1 ?lpha cell lines) of P. sylvestris leaf crude and methanol purified fractions. EXCLI J. 2016. https://doi.org/10.17179/excli2015-689
Bhardwaj K, Kumar S, Ojha S. Antioxidant activity and FT-IR analysis of Datura innoxia and Datura metel leaf and seed methanolic extracts. African J Tradit Complement Altern Med. 2016;13:7–16. https://doi.org/10.21010/ajtcam.v13i5.2
Batool R, Khan MR, Sajid M et al. Estimation of phytochemical constituents and in vitro antioxidant potencies of Brachychiton populneus (Schott & Endl.) R. Br. BMC Chem. 2019;13:1–15. https://doi.org/10.1186/s13065-019-0549
Phuyal N, Jha PK, Raturi PP, Rajbhandary S. Total phenolic, flavonoid contents and antioxidant activities of fruit, seed and bark extracts of Zanthoxylum armatum DC. Sci World J. 2020. https://doi.org/10.1155/2020/8780704
Jan S, Khan MR, Rashid U, Bokhari J. Assessment of antioxidant potential, total phenolics and flavonoids of different solvent fractions of Monotheca buxifolia fruit. Osong Public Heal Res Perspect. 2013;4:246–54. https://doi.org/10.1016/j.phrp.2013.09.003
Jadid N, Hidayati D, Hartanti SR et al. Antioxidant activities of different solvent extracts of Piper retrofractum Vahl. using DPPH assay. AIP Conf Proc. 2017; 1854. https://doi.org/10.1063/1.4985410
Zihad SMNK, Gupt Y, Uddin SJ et al. Nutritional value, micronutrient and antioxidant capacity of some green leafy vegetables commonly used by southern coastal people of Bangladesh. Heliyon. 2019:5. https://doi.org/10.1016/j.heliyon.2019.e02768
Gurung R, Adhikari S, Parajuli K. Evaluation of the antibacterial and antioxidant activity of Mimosa rubicaulis and Reinwardtia indica. Evidence-based Complement Altern. Med. 2020. https://doi.org/10.1155/2020/3862642
Ramírez-Castillo FY, Moreno-Flores AC, Avelar-González FJ et al. An evaluation of multidrug-resistant Escherichia coli isolates in urinary tract infections from Aguascalientes, Mexico: Cross-sectional study. Ann Clin Microbiol Antimicrob. 2018;17:1–13. https://doi.org/10.1186/s12941-018-0286-5.
Cinelli MA, Jones AD. Alkaloids of the genus Datura: Review of a rich resource for natural product discovery. Molecules. 2021(26). https://doi.org/10.3390/molecules26092629
Downloads
Published
Versions
- 01-04-2022 (2)
- 20-02-2022 (1)
How to Cite
Issue
Section
License
Copyright (c) 2021 Ruby George, Priti Mathur
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
