Assessment of phthalate esters (PAEs) contamination in Urtica dioica L.
DOI:
https://doi.org/10.14719/pst.1521Keywords:
Phthalic acid esters, Urtica dioica, Gas Chromatography-Mass spectrometry, Diethyl phthalate, Bis (2-ethylhexyl) phthalate, Diisobutyl phthalate, Dibutyl phthalateAbstract
Phthalate esters are a group of chemical compounds of ubiquitous nature which nowadays have become a colossal threat to the environment, human-animal and plant health, because of its higher potential of accumulation in soil and aquatic habitat leading to environmental contamination due to its widespread industrial and agricultural usage. The present research aims to analyze the phthalate esters accumulation in Urtica dioica L. For this study, the Urtica dioica is tested for the presence of phthalates by using Gas Chromatography-Mass spectrometry. The Gas Chromatography-Mass spectrometry observations show the presence of 11 phthalate esters, among which diethyl phthalate (DEP) and bis(2-ethylhexyl) phthalate (BEHP), dibutyl phthalate (DBP), and diisobutyl phthalate (DIBP) were found to be in significantly higher amount. The sum concentrations of the phthalate ester in different extracts of plant range from 16.25% to 84.07%. The % composition of diethyl phthalate is found to be comparatively higher than other phthalate esters in methanolic extract of Urtica dioica while diisobutyl phthalate and bis (2-ethylhexyl) phthalate accumulation is found relatively higher in the ethyl acetate and diethyl ether fractions. The observations show the contamination of the Urtica dioica plant with phthalate esters and also indicate the phthalate accumulating potential of the plant.
Downloads
References
Wittasek M, Koch HM, Angeror J, Bruning T. Assessing exposure to phthalates- The human biomonitoring approach. Molecular Nutrition and Food Research. 2011; Jan;55(1):7-31. https://doi.org/10.1002/mnfr.201000121
Saednia S. Phthalates. Encyclopedia of Toxicology. 2014; 3: 928-33. https://doi.org/10.1016/B978-0-12-386454-3.00963-5
Li R, Liang J, Gong Z, Zhang N, Duan H. Occurrence and spatial distribution, historical trend and ecological risk of phthalate esters in the Jiulong River, southeast China. Science of the Total Environment. 2017; Feb;580:388-97. https://doi.org/10.1016/j.scitotenv.2016.11.190
Arfaeinia H, Fazlzadh M, Taghizadeh F, Saeedi R, Spitz J, Dobaradaran S. Phthalate acid esters (PAEs) accumulation in coastal sediments from regions with different land-use configuration along the Persian Gulf. Ecotoxicology and Environmental Safety. 2019; Mar;169:496-506. https://doi.org/10.1016/j.ecoenv.2018.11.033
Wang W, Leung AOW, Chu LH, Wong MH. Phthalates contamination in China: status, trend and human exposure with an emphasis on oral intake. Environmental Pollution. 2018; Jul. 1;238:771-82. https://doi.org/10.1016/j.envpol.2018.02.088
Mi L, Xie Z, Zhao Z, Zhong M, Mi W. Occurrence and spatial distribution of phthalate esters in sediments of the Bohai and Yellow seas. Science of the Total Environment. 2019; Feb. 25;653:792-800. https://doi.org/10.1016/j.scitotenv.2018.10.438
Zhou B, Zhao L, Sun Y, Li X, Weng L, Li Y. Contamination and human health risks of phthalate esters in vegetable and crop soils from the Huang-Huai-Hai region of China. Science of the Total Environment. 2021; Jul. 15;778:146281. https://doi.org/10.1016/j.scitotenv.2021.146281
Clara M, Windhofer G, Hartt W, Braun k, Simon M, Gans O, Scheffknecht C, Chovanek. Occurrence of phthalates in surface runoff, untreated and treated wastewater fate during wastewater treatment, Chemosphere. 2010; Feb. 1;78(9):1078-84. https://doi.org/10.1016/j.chemosphere.2009.12.052
Gong M, Weschler CJ, Zhang Y. Impact of clothing on dermal exposure to phthalates: observations and insights from sampling both skin and clothing. Environment Sci Technol. 2016; Apr. 19;50:4350-57. https://doi.org/10.1021/acs.est.6b00113
Wu Q, Lam JCW, Kwok KY, Tsui MMP, Lam PKS. Occurrence and fate of endogenous steroid hormones, alkylphenol ethoxylates, bisphenol A and phthalates in sewage treatment systems. Journal of Environment Sciences. 2017; Nov. 1;61:49-58. https://doi.org/10.1016/j.jes.2017.02.021
Muller J, Kordel W. Occurrence and fate of phthalates in soil and plants, Science of The Total Environment. 1993; Jan. 1;134(1):431-37. https://doi.org/10.1016/S0048-9697(05)80044-0
Teil MJ, Blanchard M, Chevreuil M. Atmospheric fate of phthalate esters in an urban area (Paris-France). Science of The Total Environment. 2006; Feb. 1;354(2-3):212-23. https://doi.org/10.1016/j.scitotenv.2004.12.083
Erkeko?lu P, Rahidi W, Rosa VD, Giray B, Favier A, Hincal F. Protective effect of selenium supplementation on the genotoxicity of di (2-ethylhexyl) phthalate and mono(2-ethylhexyl) phthalate treatment in LNCaP cells. Free Radical Biology and Medicine. 2010;49(4):559-66. https://doi.org/10.1016/j.freeradbiomed.2010.04.038
Peropadre A, Freire PF, Martin JMP, Herrero O, Hazen MJ. Endoplasmic reticulum stress as a novel cellular response to di (2-ethylhexyl) phthalate exposure. Toxicology In Vitro. 2015; Dec. 25;30(1):281-87. https://doi.org/10.1016/j.tiv.2015.10.009
Molino C, Filippi S, Stopiello GA, Meschini R, Angeletti D. In vitro evaluation of cytotoxic and genotoxic effects of Di (2-ethylhexyl) phthalate (DEHP) on European sea bass (Dicentrarchus labrax) embryonic cell line. Toxicology In Vitro. 2019; Apr. 1;56:118-25. https://doi.org/10.1016/j.tiv.2019.01.017
Lin W, Jiang R, Xiong Y, Xu J, Zheng J, Zhu P, Ouyang G. Quantification of the combined toxic effect of polychlorinated biphenyls and nano-sized poly styrene on Daphnia magna. Journal of hazardous materials. 2019; Feb. 15; 364: 531-36. https://doi.org/10.1016/j.jhazmat.2018.10.056
Kim H, Nam K, Oh S, Son S, Jeon D, Gye MC, Shin I. Toxicological assessment of phthalates and their alternatives using human keratinocytes. Environmental Research. 2019; Aug. 1;175:316-22. https://doi.org/10.1016/j.envres.2019.05.007
Sedha S, Lee H, Singh S, Kumar S Jain S, Ahmad A et al. Reproductive toxic potential of phthalate compounds state of art review. Pharmacol Res. 2021; May. 1;167:105536. https://doi.org/10.1016/j.phrs.2021.105536
Engel SM, Miodovnik A, Canfield RL, Zhu C, Silva MJ, Calafat AM, Wolff MS. Prenatal phthalate exposure is associated with childhood be and executive functioning. Environ Health Perspect. 2010; Apr 118:565-71. https://doi.org/10.1289/ehp.0901470
Katsikantami I, Sifakis S, Tzatzarakis MN, Vakonaki E, Kalantzi OI, Tsatsakis AM et al. A global assessment of phthalates burden and related links to health effects. Environ Int. 2016; Dec. 1;97:212-36. https://doi.org/10.1016/j.envint.2016.09.013
Zarean M, Keikha M, Poursafa P, Khalighinejad P, Amin M, Kelishadi R. A systematic review on the adverse health effects of di (2-ethylhexyl) phthalate. Environ Sci Pollut Res. 2016; Dec.23(24):24642-93. https://doi.org/10.1007/s11356-016-7648-3
Liu C, Deng YL, Zheng TZ, Yang P, Jiang XQ, Liu EN et al. Urinary biomarkers of phthalate exposure and risk of thyroid cancer and benign nodule. J Haz Mat. 2020; Feb. 5;383:121189. https://doi.org/10.1016/j.jhazmat.2019.121189
Vinny. Himachal Pradesh popularising polyhouse farming. New protective cultivation technology. 2009 [Cited 4 Feb 2021]. Wordpress.com weblog. https://greenhousehimachal.wordpress.com/2009/02/09/himachal-pradesh-popularising-polyhouse-farming/
Cai QY, Mo CH, Wu QT, Zeng QY, Katsoyiannis A. Occurrence of organic contaminants in sewage sludges from the eleven wastewater treatment plants, China. Chemosphere. 2007; Aug. 1;68(9):1751-62. https://doi.org/10.1016/j.chemosphere.2007.03.041
Wang XL, Lin QX, Wang J, Lu XG, Wang GP. Effect of wetland reclamation and tillage conversion on accumulation and distribution of phthalate esters residues in soils. Ecol Eng. 2013; Feb. 1;51:10-15. https://doi.org/10.1016/j.ecoleng.2012.12.079
Gue V, Kannan K. Challenges encountered in the analysis of phthalate esters in foodstuffs and other biological matrices. Anal Bioanal Chem. 2013; Nov. 404(9):2539-54. https://doi.org/10.1007/s00216-012-5999-2
Weschler CJ, Salthammer T, Fromme H. Partitioning of phthalates among the gas phase, airborne particles and settled dust in indoor environments. Atmos Environ. 2008; Mar. 1;42(7):1449-60. https://doi.org/10.1016/j.atmosenv.2007.11.014
Zhu YY, Tian J, Yang HB, Wei EQ, Yu G, Wei FS. Phthalate pollution in atmospheric particles and soils of Tianjin and their contamination and remediation of phthalate acid esters in agricultural soils. Environ Pollut Control. 2010;31(10):1535-41.
Bodeker C, Bodeker G, Ong CK, Grundy CK, Burford G, Shein K. WHO Global Atlas of Traditional, Complementary and Alternative Medicine. Geneva, Switzerland: World Health Organization. 2005 [June 2021].
Brandão MGL, Acúrcio FA, Montemor RLM, Marlière LDP. Complementary/alternative medicine in Latin America: Use of herbal remedies among Brazilian metropolitan area population. Journal of Complementary and Integrative Medicine. 2006; Jun. 6;3(1):1-8. https://doi.org/10.2202/1553-3840.1025
Bussman RW, Sharon D, Lopez A. Blending traditional and Western medicine medicinal plants use among patients at Clinica Antigona in El Porvenir, Peru. Ethnobotany Research and Applications. 2007;5:185-99. https://doi.org/10.17348/era.5.0.185-199
Oreagba IA, Oshikoya KA, Amachree M. Herbal medicine use among urban residents in Lagos, Nigeria. BMC Complementary and Alternative Medicine. 2011; Dec;11:117-25. https://doi.org/10.1186/1472-6882-11-117
Picking D, Younger N, Mitchell S, Delgoda R. The prevalence of herbal medicine home use and concomitant use with pharmaceutical medicines in Jamaica. Journal of ethnopharmacology. 2011; Sep. 1;137(1):305-11. https://doi.org/10.1016/j.jep.2011.05.025
Warren P. 101 uses for stinging nettles. Wildeye, UK. 2006 [Nov, 2020].
Williamson EM. Potter's Herbal Cyclopaedia. CW Daniel, Saffron Walden. 2003 [Jul 2021].
El Haouari M, Rosado JA. Phytochemical, anti-diabetic and cardiovascular properties of Urtica dioica L. (Urticaceae): A Review. Mini Reviews in Medicinal Chemistry. 2019; Jan. 1;1:19(1):63-71. https://doi.org/10.2174/1389557518666180924121528
Qayyum R, Qamar HM, Khan S, Salma U, Khan T, Shah AJ. Mechanisms underlying the antihypertensive properties of Urtica dioica. Journal of translational medicine. 2016; Dec;14(1):1-3. https://doi.org/10.1186/s12967-016-1017-3
JR, Weiner MF 2019 Urtication (flogging with stinging nettles) and flagellation (beating with rods) in the treatment of paralysis. Spinal Cord Series and Cases. 2019; Sep. 26;5(1):1-5. https://doi.org/10.1038/s41394-019-0222-8
Rajput P, Sharma RA. GC Profiling of Urtica dioica for the Efficacy of Traditional Urtication Therapy. 2021; Dec;101(4):363-67.
Bayer Crop Science. Urtica dioica. 2015; [Sep 2021]. http://www.cropscience.bayer.com/en/Products-and-Innovation/Crop-Compendium/Pests-Diseases-Weeds/Weeds/Urtica-dioica.aspx
Kew Seed Information Database. Urtica dioica L. Kew, UK: Royal Botanic Gardens. 2015 [Jan 2021] http://data.kew.org/sid/SidServlet?ID=23779&Num=uLl
Dennis L. (ed.). The Ecology of Butterflies in Britain. Oxford University Press, Oxford. 1992.
USDA Forestry Service. Index of Species Information: Urtica dioica. 2009.
USDA-NRC. The PLANTS Database. Baton Rouge, USA: National Plant Data Center. 2015.http://plants.usda.gov/
Robinson T, McMullan G, Marchamt R, Nigam P. Remediation of dyes in textile effluent: a critical review on current treatment technologies with a proposed alternative. Bioresource Technology. 2001; May. 1;77(3):247-55. https://doi.org/10.1016/S0960-8524(00)00080-8
Macek T, Kotrba P, Svatos A, Novakova M, Demnerova K, Mackova M. Novel roles for genetically modified plants in environmental protection. Trends in Biotechnology. 2008; Mar. 1;26(3):146-52. https://doi.org/10.1016/j.tibtech.2007.11.009
Viktorova J, Jandova Z, Madlenakova M, Prouzova P, Batunek V. Native phytoremediation potential of Urtica dioica for removal of PCBs and heavy metals can be improved by genetic manipulations using constitutive CaMV 35S promoter. PLOS ONE. 2017, Dec 8;12(10):e0187053. https://doi.org/10.1371/journal.pone.0187053
Shams KM, Tichy G, Fischer A, Sager M, Peer T, Bashar A, Filip K. Aspects of phytoremediation for chromium contaminated sites using common plants Urtica dioica, Brassica napus and Zea mays. Plant and Soil. 2010; Mar;328:175-89. https://doi.org/10.1007/s11104-009-0095-x
Li RW, Leach DN, Myers P, Leach GJ, Lin GD, Brushett DJ, Waterman PG. Anti-inflammatory activity, cytotoxicity and active compounds of Tinospora smilacina Benth. Phytother Res. 2004;18:78-83. https://doi.org/10.1002/ptr.1373
Dar SA, Yousuf AR, Ganai FA, Sharma P, Kumar N, Singh R. Bioassay-guided isolation and identification of anti-inflammatory and anti-microbial compounds from Urtica dioica L. (Urticaceae) leaves. African Journal of Biotechnology. 2012;11(65):12910-20. https://doi.org/10.5897/AJB11.3753
Mahmoudi R, Amini K, Fakhri O, Alem M. Aroma profile and antimicrobial properties of alcoholic and eques extracts from root, leaves and stock of nettle (Urtica dioica L.). Journal of Microbiology Biotechnology and Food Sciences. 2014; Jan. 6;4(3):220-24. https://doi.org/10.15414/jmbfs.2014-15.4.3.220-224
Zehraw HM, Muotaz Z, Taleb M, Mukhlif B, Al SA, Al JI. Extraction and evaluation of the activity of Urtica dioica as bleeding stop material. Iraqi Journal of Biotechnology. 2019; Sep. 4;18(2):77-87.
Mayer FL, Stalling DLS, Johnson JL. Phthalate esters as environmental contaminants. Nature. 1972; Aug;238:411-13. https://doi.org/10.1038/238411a0
Wang J, Chen G, Christie P, Zhang M, Luo Y, Teng Y. Occurrence and risk assessment of phthalate esters (PAEs) in vegetable and soils of suburban plastic film greenhouses. Science of The Total Environment. 2015; Aug. 1; 523: 129-37. https://doi.org/10.1016/j.scitotenv.2015.02.101
Mesdaghinia A, Azari A, Nodehi R, Yeghmaeian K, Bharti A, Agarwal S. Investigation on the magnetic absorption separation, catalytic degradation and toxicity bioassay. Journal of Molecular Liquids. 2017;233:378-90. https://doi.org/10.1016/j.molliq.2017.02.094
Blount BC, Silva MJ, Caudill SP, Needham LL. Levels of seven urinary phthalate metabolites in a human reference population. Environ Health Perspect. 2000;Oct;108(10):979-82. https://doi.org/10.1289/ehp.00108979
Koch HM, Rossbach B, Drexler H, Angerer J. Internal exposure of the general population to DEHP and other phthalates- determination of secondary and primary monoester metabolite in urine. Environ Res. 2003; Oct. 1;93:177-85. https://doi.org/10.1016/S0013-9351(03)00083-5
Silva MJ, Barr DB, Reidy JA, Malek NA. Urinary levels of seven phthalate metabolites in the US population from the National Health and Nutrition Examination Survey (NHANES) 1999-2000. Environ Health Perspect. 2004; Mar;112:331-38. https://doi.org/10.1289/ehp.6723
Wittassek M, Wiesmuller GA, Koch HM, Eckard R. Internal phthalate exposure over the last two decades- a retrospective human biomonitoring study. Int J Hyg Environ Health. 2007; May. 22;210:319-33. https://doi.org/10.1016/j.ijheh.2007.01.037
Bauer MJ, Herrmann R. Estimation of the environmental contamination by phthalic acid esters leading from household wastes. Science of The Total Environment. 1997; Dec. 3;208:49-57. https://doi.org/10.1016/S0048-9697(97)00272-6
ECB. European Union risk assessment Report for dibutyl phthalate (with addendum 2004), European Chemicals Bureau, Ispra, Italy.2004; [Jun 2021].
ECB. European Union Risk Assessment Report for Bis (2-ethylhexyl) phthalate (Final Report 2008), European Chemicals Bureau, Ispra, Italy. 2008.
Kavlock R, Boekelheide K, Chapin R, Cunningham M. NTP Center for the Evaluation of Risks to Human Reproduction: phthalates expert panel report on the reproductive and developmental toxicity of di-n-octyl phthalate. Reprod Toxicol. 2002;16:721-34. https://doi.org/10.1016/S0890-6238(02)00031-X
Kavlock R, Boekelheide K, Chapin R, Cunningham M. NTP Center for the Evaluation of Risks to Human Reproduction: phthalates expert panel report on the reproductive and developmental toxicity of di(2-ethylhexyl) phthalate. Reprod Toxicol. 2002;16:529-653. https://doi.org/10.1016/S0890-6238(02)00032-1
Kavlock R, Barr D, Boekelheide K, Breslin W. NTP-CERHR expert panel update on the reproductive and developmental toxicity of di (2-ethylhexyl) phthalate. Reprod Toxicol. 2006; Oct. 1;16:291-399. https://doi.org/10.1016/j.reprotox.2006.04.007
Borch J, Axelstad M, Vinggaard AM, Dalgaard M. Diisobutyl phthalate has comparable anti-androgenic effects to di-n-butyl phthalate in fetal rat testis. Toxicol Lett. 2006; Jun. 1;163:183-90. https://doi.org/10.1016/j.toxlet.2005.10.020
Saillenfait, AM, Sabate JP, Gallissot F. Developmental toxic effects of diisobutyl phthalate, the methyl-branched analogue of di-n-butyl phthalate, administered by gavage to rats. Toxicol Lett. 2006; Aug. 1;165(1):39-46. https://doi.org/10.1016/j.toxlet.2006.01.013
Saillenfait AM Sabate JP, Gallissot F. Diisobutyl phthalate impairs the androgen-dependent reproductive development of the male rat. Reprod Toxicol. 2008; Oct. 1; 26:107-15. https://doi.org/10.1016/j.reprotox.2008.07.006
Gray LE, Ostby J, Furr J, Price M. Perinatal exposure to the phthalates DEHP, BBP and DINP, but not DEP, DMP or DOTP, alters sexual differentiation of the male rat. Toxicol Sci. 2000; Dec. 1;58:350-65. https://doi.org/10.1093/toxsci/58.2.350
Parks LG, Ostby JS, Lambright, CR, Abbott BD. The plasticizer diethylhexyl phthalate induces malformations by decreasing fetal testosterone synthesis during sexual differentiation in the male rat. Toxicol Sci. 2000; Dec. 1;58:339-49. https://doi.org/10.1093/toxsci/58.2.339
Andrade AJ, Grande SW, Talsness CE, Gericke C. A dose-response study following in utero and lactational exposure to di- (2-ethylhexyl) phthalate (DEHP): reproductive effects on adult male offspring rats. Toxico;228:85-97. https://doi.org/10.1016/j.tox.2006.08.020
Nagao T, Ohta R, Marumo H, Shindo T. Effect of butyl benzyl phthalate in Sprague Dawley rats after gavage administration: a two-generation reproductive study. Reprod Toxicol. 2000; Nov. 1;14:513-32. https://doi.org/10.1016/S0890-6238(00)00105-2
Tyl RW, Myers CB, Marr MC, Fail PA. Reproductive toxicity evaluation of dietary butyl benzyl phthalate (BBP) in rats. Reprod Toxicol. 2004; Mar. 1;18:241-64. https://doi.org/10.1016/j.reprotox.2003.10.006
Sharpe RM, Irvine DS. How strong is the evidence of a link between environmental chemicals and adverse effects on human reproductive health ? Br Med J. 2004; Feb. 19;328:447-51. https://doi.org/10.1136/bmj.328.7437.447
Foster PMD. Disruption of reproductive development in male rat offspring following in utero exposure to phthalate esters. Int J Androl. 2006; Feb;29:140-47. https://doi.org/10.1111/j.1365-2605.2005.00563.x
Howdeshell KL, Rider CV, Wilson VS, Gray LE. Mechanisms of action of phthalate esters, individually and in combination, to induce abnormal reproductive development in male laboratory rats. Environ Res. 2008; Oct. 1;108:168-76. https://doi.org/10.1016/j.envres.2008.08.009
Wilson VS, Blystone CR, Hotchkiss AK, Rider CV. Diverse mechanisms of anti-androgen action: impact on male rat reproductive tract development. Int J Androl. 2008; Apr;31:178-87. https://doi.org/10.1111/j.1365-2605.2007.00861.x
Bao J, Wang M, Ning X, Zhou Y, He Y, Yang J, Gao X, Li S, Ding Z, Chen B. Phthalate concentrations in personal care products and cumulative exposure to female adults and infants in Shanghai. Journal of Toxicology and Environmental Health. 2015; Mar. 4;78(5):325-41. https://doi.org/10.1080/15287394.2014.968696
Wu Z, Zhang X, Wu X, Shen G, Du Q, Mo C. Update of di (2-ethylhexyl) phthalate DEHP by the plant Beninoasa hispida and its use for lowering DEHP content of intercropped vegetables. Journal of Agricultural and Food Chemistry. 2013; Jun. 5;61(22):5220-25. https://doi.org/10.1021/jf401378u
Downloads
Published
Versions
- 01-07-2022 (2)
- 10-05-2022 (1)
How to Cite
Issue
Section
License
Copyright (c) 2022 Priyanka Rajput, R. A Sharma
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).