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Plant Science Today (PST)

Research Articles

Vol. 9 No. 3 (2022)

Morpho-anatomical responses of Catharanthus roseus due to combined heavy metal stress observed under Scanning Electron Microscope

DOI
https://doi.org/10.14719/pst.1621
Submitted
9 December 2021
Published
13-05-2022 — Updated on 01-07-2022
Versions

Abstract

Heavy metals trigger various plant responses that basically vary with the intensity as well as duration of stress. Comprehension of the morphological and anatomical responses to such stress is essential for a holistic perception of plant resistance mechanisms to metal-excess conditions in higher plants. In the present study, the effects of heavy metals on morpho-anatomy of Catharanthus roseus based on its potential to tolerate metal stress has been studied in industrially polluted environments. The tissue samples of these plants grown in contaminated and uncontaminated soils were processed for analysis under Scanning Electron Microscope (SEM). Briefly, harvested tissues were pre-fixed using glutaraldehyde and paraformaldehyde in sodium cacodylate (CAC) buffer, followed by post fixation in osmium tetroxide. Further, the digital micrographs of critically dried samples were captured. The analysis of micrographs revealed structural changes like cell wall thickening, increased stele diameter, increased root and shoot diameter, variations in stomatal number, enlargement of trichomes and salt glands of plants grown in contaminated soil when compared to those grown in uncontaminated soil. The study also provided microscopic evidence of endophytic colonization of microorganisms within surface-disinfected plant tissues. Based on the varied morpho-anatomical responses due to heavy metal stress, several physiological and metabolic mechanisms of plants were deciphered.

References

  1. Tatiana M, Grigoriy F, Dina N, Aleksei F, Victor C, Saglara M. Morphological and anatomical changes of Phragmites australis Cav. due to the uptake and accumulation of heavy metals from polluted soils. Science of The Total Environment. 2018; 636: 392-401. https://doi.org/10.1016/j.scitotenv.2018.04.306.
  2. Vahid T. Interactive effects of zinc and boron on growth, photosynthesis and water relations in pistachio. Journal of Plant Nutrition. 2017; 40(11): 1588-1603. https://doi.org/10.1080/01904167.2016.1270308
  3. Xinyi C, Mingyan J, Jiarong L, Yixiong Y, Ningfeng L, Qibing C, et al. Biomass allocation strategies and Pb-enrichment characteristics of six dwarf bamboos under soil Pb stress. Ecotoxicology and Environmental Safety. 2021;207: 111500. https://doi.org/10.1016/j.ecoenv.2020.111500.
  4. Laura YE, Roberto BG, Joel F, Elizabeth AC. Effect of heavy metals on seed germination and seedling development of Nama aff. stenophylla collected on the slope of a mine tailing dump. International Journal of Phytoremediation. 2020;22(14):1448-61. https://doi.org/10.1080/15226514.2020.1781782
  5. Fernandez DM, Walker DJ, Espinar PR, Flores P, Río JA. Physiological responses of Bituminaria bituminosa to heavy metals. Journal of Plant Physiology. 2011;168(18):2206-11. https://doi.org/10.1016/j.jplph.2011.08.008.
  6. Disante KB, Fuentes D, Cortina J. Response to drought of Zn-stressed Quercus suber L. seedlings. Environmental and Experimental Botany. 2011; 70(2-3): 96-103. https://doi.org/10.1016/j.envexpbot.2010.08.008.
  7. Mittler R. Abiotic stress, the field environment and stress combination. Trends in Plant Science. 2006;11(1):15-19. https://doi.org/10.1016/j.tplants.2005.11.002.
  8. Soumya V, Sowjanya A, Kiranmayi P. Evaluating the status of phytochemicals within Catharanthus roseus due to higher metal stress. International Journal of Phytoremediation. 2021;23(13):1391-1400. https://doi.org/10.1080/15226514.2021.1900063.
  9. Pathan AK, Bond J, Gaskin RE. Sample preparation for scanning electron microscopy of plant surfaces—Horses for courses. Micron. 2008; 39(8): 1049-61. https://doi.org/10.1016/j.micron.2008.05.006.
  10. Asma A, Wajid Z, Fazal U, Saddam S, Shayan J, Saraj B, Abdul S, Bushra A. Systematics study through scanning electron microscopy; a tool for the authentication of herbal drug Mentha suaveolens Ehrh. Microscopy Research and Technique. 2020;83(1):81-87. https://doi.org/10.1002/jemt.23391
  11. Schneider C, Rasband W, Eliceiri K. NIH Image to ImageJ: 25 years of image analysis. Nat Methods. 2012;9:671-75. https://doi.org/10.1038/nmeth.2089
  12. Filotheou HP, Bosabalidis AM, Karataglis S. Effects of Copper Toxicity on Leaves of Oregano (Origanum vulgare subsp. hirtum). Annals of Botany. 2001;88(2):207-14. https://doi.org/10.1006/anbo.2001.1441.
  13. Vaishali Y, Namira A, Ján K, Vijay PS, Durgesh KT, Devendra KC, Marek V. Structural modifications of plant organs and tissues by metals and metalloids in the environment: A review. Plant Physiology and Biochemistry. 2021; 159: 100-12. https://doi.org/10.1016/j.plaphy.2020.11.047.
  14. Drake PL, Froend RH, Franks PJ. Smaller, faster stomata: scaling of stomatal size, rate of response and stomatal conductance. Journal of Experimental Botany. 2013;64(2):495-505. https://doi.org/10.1093/jxb/ers347
  15. Karabourniotis G, Liakopoulos G, Nikolopoulos D, Bresta P. Protective and defensive roles of non-glandular trichomes against multiple stresses: structure–function coordination. Journal of Forestry Research. 2020;31:1-12. https://doi.org/10.1007/s11676-019-01034-4
  16. Kalsoom B, Aamir S, Usman A, Asad U, Zahoor UH, Brekhna G, Inam Uh, Sunera, Mehrina A.Foliar micromorphology and its role in identification of the Apocynaceae taxa. Microscopy Research and Technique. 2020;83(7):755-66. https://doi.org/10.1002/jemt.23466
  17. Maheshi D, John CL. Making plants break a sweat: the structure, function and evolution of plant salt glands. Frontiers in Plant Science. 2017; 8: 406. https://doi.org/10.3389/fpls.2017.00406
  18. Castaneda TG, Brown KM, Lynch JP. Reduced root cortical burden improves growth and grain yield under low phosphorus availability in maize. Plant, Cell and Environment. 2018; 41(7):1579-92. https://doi.org/10.1111/pce.13197
  19. Rucinska SR. Water relations in plants subjected to heavy metal stresses. Acta Physiologiae Plantarum. 2016; 38: 257. https://doi.org/10.1007/s11738-016-2277-5
  20. Yeats TH, Rose JK. The formation and function of plant cuticles. Plant Physiology. 2013; 163: 5-20. https://doi.org/10.1104/pp.113.222737.
  21. Kim H, Choi D, Suh MC. Cuticle ultrastructure, cuticular lipid composition, and gene expression in hypoxia-stressed Arabidopsis stems and leaves. Plant Cell Rep. 2017; 36: 815-27. https://doi.org/10.1007/s00299-017-2112-5
  22. Leite DCC, Grandis A, Tavares EQP, Piovezani AR, Pattathil S, Avci U, Rossini A, Cambler A, DeSouza AP, Hahn MG, Buckeridge MS. Cell wall changes during the formation of aerenchyma in sugarcane roots. Annals of Botany. 2017; 120(5): 693-708. https://doi.org/10.1093/aob/mcx050
  23. Cuimin G, Min W, Lei D, Yupei C, Zhifeng L, Jun H, Shiwei G. High water uptake ability was associated with root aerenchyma formation in rice: Evidence from local ammonium supply under osmotic stress conditions. Plant Physiology and Biochemistry. 2020;150:171-79. https://doi.org/10.1016/j.plaphy.2020.02.037.
  24. Mahasin AK, Meghma B, Robert AS, Teresa EVS, Subir B. Evidence of simultaneous occurrence of tylosis formation and fungal interaction in a late Cenozoic angiosperm from the eastern Himalaya. Review of Palaeobotany and Palynology. 2018;259:171-84. https://doi.org/10.1016/j.revpalbo.2018.10.004.
  25. Shanying HE, Xiaoe Y, Zhenli HE, Virupax CB. Morphological and Physiological Responses of Plants to Cadmium Toxicity: A Review. Pedosphere. 2017; 27(3): 421-38. https://doi.org/10.1016/S1002-0160(17)60339-4.
  26. Pugsley MK, Tabrizchi R. The vascular system: An overview of structure and function. Journal of Pharmacological and Toxicological Methods. 2000; 44(2): 333-40. https://doi.org/10.1016/S1056-8719(00)00125-8.
  27. Bengough AG, Bransby MF, Hans J, McKenna SJ, Roberts TJ, Valentine TA. Root responses to soil physical conditions; growth dynamics from field to cell. Journal of Experimental Botany. 2006; 57(2): 437-47. https://doi.org/10.1093/jxb/erj003.

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