Free radical scavenging activity, pancreatic lipase and a-amylase inhibitory assessment of ethanolic leaf extract of Phyllanthus amarus
DOI:
https://doi.org/10.14719/pst.1809Keywords:
Diabetes, Medicinal plant, Obesity, Oxidative stressAbstract
Oxidative stress is associated with the pathogenesis of diabetes and obesity. The key enzymes involved in the carbohydrate and lipid metabolisms are a-amylase and pancreatic lipase respectively. The phytochemicals present in medicinal plants are known to be potent free radical scavenging agents and great inhibitors of metabolizing enzymes. Phyllanthus amarus leaves have antioxidant, anti-diabetic, and anti-obesity properties. This research is aimed at elucidating the carbohydrate and lipid metabolism inhibitory activities of P. amarus leaves through in-vitro screening. The phytochemical screening revealed that P.amarus is having high phenolic, flavonoid, tannin, and saponin content. The total protein content of P.amarus showed 2.720±0.001 mg BSAE/g. Diphenyl-2-picrylhydrazyl (DPPH) assay showed 0.0001mg/mL, Nitric oxide assay revealed 0.452 mg/mL, Ferric Reducing Antioxidant Power (FRAP) assay showed a value of 0.132±0.010 mg RE/g, Total antioxidant capacity (TAC) indicated a value of 2.217±0.002 mg GAE/g and 2,2-azino-bis(3-ethylbenzotiazoline)-6-sulfonic acid (ABTS) inhibitory activity at the peak concentration (1.2 mg/mL) was 40.29%. The enzyme inhibitory assessment showed a decrease in a-amylase activity as the concentration increased while there was a slight decrease in a-lipase activity but a slight increase at 18.75 mg/mL.
Downloads
References
Temidayo O, Mutiyat A, Solomon I, Oreoluwa, D. Phyllanthusamarus extract restored deranged biochemical parameters in rat model of hepatotoxicity and nephrotoxicity, Heliyon, 2020; 6. https://doi.org/10.1016/j.heliyon.2020.e05670
Prior R, Cao G. Antioxidant capacity and polyphone compounds of teas. Proceedings of the Society for Experimental Biology and Medicine. 1999; 220:255–261. https://doi.org/10.1046/j.1525-1373.1999.d01-44.x
Azab AE, Albasha MO, Elsayed ASI. Prevention of nephropathy by some natural sources of antioxidants. Yangtze Medicine. 2017; 1: 235– 266.https://doi.org/10.4236/ym.2017.14023
Sanaberg F, Corrigan D. Natural Remedies. Their origin and uses. Taylor and Francis, Abingdon. 2001. ISBN 9780415272025. https://doi.org/10.4324/9780203302996
Ogunlana OO, Adetuyi BO, Esalomi EF, Rotimi MI, Popoola JO, Ogunlana OE, Adetuyi OA. Antidiabetic and Antioxidant Activities of the Twigs of Andrograhispaniculata on Streptozotocin-Induced Diabetic Male Rats. BioChem. 2021 1(3):238-249. https://doi.org/10.3390/biochem1030017
Shaw JE, Sicree RA, Zimmet PZ.Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Res Clin Pract. 2010; 87(1):4-14. doi: 10.1016/j.diabres.2009.10.007
International Diabetes Federation 2013. IDF Diabetes Atlas 6th ed. IDF: Brussels, ISBN 2-930229-85-3 (online version at www.idf.org/diabetesatlas (accessed on 24.07.14.)
Kumar V, Makkar H, ; Becker K. Detoxified Jatropha curcas kernel meal as a dietary protein source: Growth performance, nutrient utilization and digestive enzymes in common carp (Cyprinus carpio L.) fingerlings. Aquacult. Nutr. 2011; 17 (3): 313-326. https://doi.org/10.1111/j.1365-2095.2010.00777.x/pdf
World Health Organization. Obesity and overweight. 2015
Ogunlana OO, Ogunlana OE, Adekunbi TS, Adetuyi BO, Adegboye BE, and Iheagwam FN. Anti-inflammatory Mechanism of Ruzu Bitters on Diet-Induced Nonalcoholic Fatty Liver Disease in Male Wistar Rats. Evidence-Based Complementary and Alternative Medicine. 2020; https://doi.org/10.1155/2020/5246725
Anstey KJ, Cherbuin N, Budge M, Young J. Body mass index in midlife and late-life as a risk factor for dementia: a meta-analysis of prospective studies. Obesity Reviews, 2011; 12 (5): e426–37. https://doi.org/10.1111/j.1467-789X.2010.00825.x
Singh M, Tiwari N, Shanker K, Verma R, Grupta A, Grupta. Two new lignans from P. amarus. Journal of Asian Natural Products Research. 2009; 11: 562-568 https://doi.org/10.1016/j.jep.2011.09.040
Czernichow S, Kengne AP, Stamatakis E, Hamer M, Batty G. Body mass index, waist circumference and waist-hip ratio: which is the better discriminator of cardiovascular disease mortality risk?: evidence from an individual-participant meta-analysis of 82 864 participants from nine cohort studies. Obesity Reviews, 2011; 12(9): 680–7. https://doi.org/10.1111/j.1467-789X.2011.00879.x. Epub 2011
Adetuyi BO, Okeowo TO, Adetuyi OA, Adebisi OA, Ogunlana O, Oretade OJ, Marraiki N, Beshbishy AM, Welson NN, Batiha GE. Ganoderma lucidum from red mushroom attenuates formaldehyde- induced liver damage in experimental male rat model. Biology2020;9(10): 313. https://doi.org/10.3390/biology9100313
Anandacoomarasamy A, Caterson I, Sambrook P, Fransen M, March L. The impact of obesity on the musculoskeletal system. The International Journal of Obesity. 2008; 32(22):11–22. https://doi.org/10.1038/sj.ijo.0803715
Kim H, Kang M. Screening of Korean medicinal plants for lipase inhibitory activity. Phytotherapy Research. 2005; 19: 359-61. https://doi.org/10.1002/ptr.1592
Ogunlana OO, Adetuyi BO, Adekunbi TS, Adegboye BE, Iheagwam FN, Ogunlana OE. Ruzu bitters ameliorates high–fat diet induced non-alcoholic fatty liver disease in male Wistar rats. Journal of Pharmacy and Pharmacognosy Research 2021;9(3):251-26https://jppres.com/jppres/pdf/vol9/jppres20.868_9.3.251.pdf
Moreno D, Ilic N, Poulev A,Raskin A. Effects of Arachis hypogaea nutshell extract on lipid metabolic enzymes and obesity parameters. Life Sciences. 2006; 78:2797-803. https://doi.org/10.1016/j.lfs.2005.11.012
Shimoda H, Seki E, Aitani M. Inhibitory effect of green coffee bean extract on fat accumulation and body weight gain in mice. BMC Complement Alternative Medicine. 2006; 6: 9. https://doi.org/10.1186/1472-6882-6-9
Adetuyi BO, Dairo JO, Didunyemi MO. Anti-Hyperglycemic Potency of Jatropha Gossypiifolia in Alloxan Induced Diabetes. BiochemPharmacol(Los Angel)2015; 4(5):193.doi:10.4172/2167-0501.1000193
Ogunlana OO, Adetuyi BO, Rotimi M, Esalomi I, Adeyemi A, Akinyemi J, Ogunlana O, Adetuyi O, Adebisi O, Okpata E, Baty R, Batiha G. Hypoglycemic Activities of Ethanol Seed Extract of Hunteria umbellate (Hallier F.) on Streptozotocin-induced Diabetic Rats. Clinical Phytoscience 2021;7 (1): 1-9doi: 10.1186/s40816-021-00285-1
Pius O, Babawale O, Sola E, Comfort, O. A comparative study of nutritional and phytochemical composition of Phyllanthus amarus leaf and seed’. American Journal of Pharmacology and Toxicology. 2015; 7: 321–327. https://doi.org/10.5829/idosi.aejts.2015.7.4.9699
Champe P, Harvey R, Ferrier D. Biochemistry. Lippincott Williams and Wilkins. 2005, ISBN 978-1-60831-412-6
Adetuyi BO, Adebisi OA, Awoyelu EH, Adetuyi OA,Ogunlana OO. Phytochemical and Toxicological effect of Ethanol extract of Heliotropium indicum on Liver of Male Albino Rats. Letters in Applied NanoBioscience (LIANB) 2020; 10(2):2085-2095 https://doi.org/10.33263/LIANBS102.20852095
Iheagwam F, Nsedu E, Kazeem O, DeCampos O, Ogunlana, O, Chinedu, S. Nauclea latifolia Sm. leaf extracts extenuate free radicals, inflammation, and diabetes-linked enzymes. Oxidative Medicine and Cellular Longevity. 2020;1: 13. https://doi.org/10.1155/2020/5612486
Adetuyi BO, Omolabi FK, Olajide PA, Oloke JK. Pharmacological, Biochemical and Therapeutic Potential of Milk Thistle (Silymarin): A Review World News of Natural Sciences 2021;37:75-91
Jamuna S, Paulsamy S, Karthika, K. Phytochemical analysis and evaluation of leaf and root parts of the medicinal herbs, Hypochaerisradicata for in vitro antioxidant activities. Asian Pacific Journal of Tropical Biomedicine. 2014; 4(1): 359-367. https://doi.org/10.12980/APJTB.4.2014C1030
Jaiswal D, Rai P, Kumar A, Mehta S, Watal, G. Effect of Moringa oleifera lam. Leaves aqueous extract therapy on hyperglycemic rats. Journal of Etnopharmacology. 2009; 123: 392–6.http://dx.doi.org/10.1016/j.jep.2009.03.036
Ogunlana OO, Ogunlana OE, Popoola JO, Adetuyi BO, Adekunbi TS, David OL, Adeleye OJ, Udeogu SA, Adeyemi AO. Twigs of Andrographis paniculata (Burn. F) Nees attenuates Carbon Tetrachloride (CCl4) Induced Liver Damage in Wistar Albino Rats. RASAYAN Journal of Chemistry 2021;14(4): 2598-2603 https://doi.org/10.31788/RJC.2021.1445987
Ebrahimzadeh M, Nabavi S, Nabavi F,Bekhradnia, A. Antioxidant and free radical scavenging activity of H. officinalis L. var. angustifolius, V. odorata, B. hyrcana and C. speciosum.Pakistan Journal of Pharmaceutical Sciences. 2010; 23(1): 29–34.
Adetuyi BO, Toloyai TY, Ojugbeli ET, Oyebanjo OT, Adetuyi OA, Uche OZ, Olisah MC, Adumanya OC, Jude C, Chikwendu JK, Akram M, Awuchi CG, Egbuna C. Neurorestorative Roles of Microgliosis and Astrogliosis in Neuroinflammation and Neurodegeneration. Scicom Journal of Medical and Applied Medical Sciences2021;1(1):1-5https://doi.org/10.54117/sjmams.v1i1.1
Iheagwam F, Nsedu E, Kazeem O, DeCampos O, OgunlanaO, Chinedu S. GC-MS Analysis and Inhibitory Evaluation of Terminalia catappa Leaf Extracts on Major Enzymes Linked to Diabetes Evidence-Based Complementary and Alternative Medicine. 2019: 14. https://doi.org/10.1155/2019/6316231
Anyanwu G, Hafiz M, Jamshed I, Khalid Rauf.Ethylacetate Fraction of Anthocleista vogelii Planch Demonstrates Antiobesity Activities In Preclinical Models. Tropical Journal of Pharmaceutical Research. 2015; 18(3): 547-554 https://doi.org/10.4314/Tjpr.V18i3.15
Ogbera A, Dada O, Adeyeye F, Jewo P. Complementary and alternative medicine use in diabetes mellitus. West African Journal of Medicine. 2010; 29(3): 158-62. https://doi.org/10.4314/wajm.v29i3.68213
Hasenah Ali , Houghton P, Amala S. alpha-Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. Journal of Ethnopharmacology. 2006; 107(3): 449-55 http://dx.doi.org/10.1016/j.jep.2006.04.004
Didunyemi MO, Adetuyi BO, Oyewale IA. Inhibition of lipid peroxidation and in-vitro antioxidant capacity of aqueous, acetone and methanol leaf extracts of green and red Acalypha wilkesiana Muell. Arg. International Journal of Biological & Medical Research . 2020; 11(3):7089- 7094
Kussuya C, Daniella V, Lucilia G, Jao B. Antiorganic solvents provides a higher efficiency in extracting inflammatory properties of extract, fractions and compounds for antimicrobial activities compared to water lignans isolated from Phyllanthus amarus. Planta Med. 2005 71(8):721-6. http://dx.doi.org/10.1055/s-2005-871258.
James-Okoro PO, Iheagwam FN, Sholeye MI, Umoren IA, Adetuyi BO, Ogundipe AE, Braimah AA, Adekunbi TS, Ogunlana OE, Ogunlana OO. Phytochemical and in vitro antioxidant assessment of Yoyo bitters. World News of Natural Sciences 2021;37:1-17
Nwankpa P, Chuwuemeka O, Uloneme G, Etteh C, Ugwuwzumba P, Nwosu D. Phyto-nutrient composition and antioxidative potential of ethanolic leaf extract of sida acuta in Wistar Albino Rats. African Journal of Biotechnology 14(49):3264-3269. http://dx.doi.org/10.5897/AJB2015.14897
Downloads
Published
Versions
- 01-04-2023 (2)
- 04-02-2023 (1)
How to Cite
Issue
Section
License
Copyright (c) 2022 Ogundipe Adebanke , Adetuyi Babatunde, Iheagwam Franklyn, Adefoyeke Keleeko, Olugbuyiro Joseph, Ogunlana Olubanke
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
