Bio synthesis, Characterization of ZnO Nanoparticles from Scoparia dulcis L. plant extract and its in-vitro Antioxidant, Acetylcholinesterase Activity
DOI:
https://doi.org/10.14719/pst.2003Keywords:
Scoparia dulcis, SDE –ZnO Nps, SEM, TEM, XRD, EDAX, Acetylcholinesterase (AChE) Activity, DPPH, ABTS assayAbstract
The current investigation is focused on the use of green synthesis methods for zinc oxide nanoparticles (ZnO NPs) from Scoparia dulcis L. extract (SDE). SDE-mediated ZnO NPs (SDE-ZnO-NPs) were made using a simple and eco-friendly method that required little reaction time and calcinations temperature. UV-Vis, FTIR, X-ray powder diffraction, SEM, TEM & EDAX were used into characterizes the skeletal and synthetic properties concerning biosynthesized ZnO nonmaterial. The UV–Visible spectroscopy absorption peak for SDE-ZnO-NPs was found to be at 380 nm, confirming the production of ZnO NPs. The FTIR spectrum also revealed bioactive functional groups as well as metal-oxygen groups. Synthesized ZnO NPs had a rod shape in 200 nm, according to TEM examination. The Zn and O in the produced ZnO NPs were approved by the EDAX analysis. The XRD results revealed that it had a crystal structure that was similar to SDE-ZnO-NPs. The dose-related Acetylcholinesterase inhibitory action of SDE-ZnO NPs was determined utilizing the Ellman’s test. AChE activity of the synthesized nanoparticles showed potential inhibitory activity with IC50 values of 75.34 µg/mL. The antioxidant activity was investigated the biosynthesized ZnO-NPs using DPPH, ABTS assay.
Downloads
References
Masaru K, Toshimitsu H, Munehisa A, Naokata MH. Phytochemistry. 1988;27(11):3709-11. https://doi.org/10.1016/0031-9422(88)80811-2
Silink M. Endocrinology. 2011;2007(1):12-14. https://doi.org/10.17925/EE.2007.00.01.14
Rajan R, Vedi M, Sridharan B, Himaja M, Sabina EP, Raj NAN. Int J Phytomed. 2014;6(4):617-24.
Zikang Jiang, Jinghui Sung, Xuyun Wang, Yangyang Zhang, Yaomiao Wang, Haifeng Zhoua, Lei Wen. A review on the phytochemistry and pharmacology of the herb Scoparia dulcis L. for the potential treatment of metabolic syndrome. RSC Adv. 2021;11:31235-59. https://doi.org/10.1039/D1RA05090G
Yingbo Yang, Zhuzhen Han, Tong Tian, Qi Liao, JiaranGeng, Ying Xiao, Chemical constituents from aerial parts of Scoparia dulcis. Chinese Herbal Medicines. 2021. https://doi.org/10.1016/j.chmed.2021.12.007
Ahmed R, Ibrahim H, Yakubu M.I, Dickson P.B. Phytochemical and anti-inflammatory studies on methanol leaf extract of Scoparia dulcis Linn. Afr J Biomed Res. 2022; 25(2):257-60. https://doi.org/10.4314/ajbr.v25i2.21%20
Netnapa Chana,Thitinan Aiebchun, Pattranit Pinwanit and Asadhawut Hiranrat, The protective role of Scoparia dulcis Linn. in alzheimer’s disease. Trends in Sciences. 2022;19(2):2050;1-11. https://doi.org/10.48048/tis.2022.2050
Ramsden J. Nanotechnology: An Introduction; William Andrew. 2016. https://doi.org/10.1016/B978-0-323-39311-9.00017-0
Kalyani Khanra, Sudipta Panja, Indranil Choudhuri, Anindita Chakraborty, Nandan Bhattacharyya. Evaluation of antibacterial activity and cytotoxicity of green synthesized silver nanoparticles using Scoparia dulcis. Nano Biomed. Eng. 2015;7(3):128-33. http://dx.doi.org/10.5101/nbe.v7i3.p128-133
Dai Hai Nguyen, Jung Seok Lee, Ki Dong Park, Yern Chee Ching, Xuan Thi Nguyen,
Giang Phan, Thai Thanh Hoang Thi. Green silver nanoparticles formed by Phyllanthus urinaria, Pouzolzi azeylanica and Scoparia dulcis leaf extracts and the antifungal activity. Nanomaterials. 2019;10(3):542;1-4. https://doi.org/10.3390/nano10030542
Radhika Parvataneni, Biogenic synthesis and characterization of silver nanoparticles using
aqueous leaf extract of Scoparia dulcis L. assessment of their antimicrobial property. Drug and Chemical Toxicology. 2019;1-15. https://doi.org/10.1080/01480545.2018.1505903
Ngoc ThuyTrang Le, Dai Hai Nguyen, Ngoc Hoi Nguyen, Yern Chee Ching, Dong Yen Pham Nguyen, Cuong Quoc Ngo et al. Silver nanoparticles ecofriendly synthesized by Achyranthes aspera and Scoparia dulcis leaf broth as an effective fungicide. Applied Sciences. 2020;10(7):2505;1-14. https://doi.org/10.3390/app10072505
Meghana Navada K, Nagaraja G.K, Josline Neetha D’Souza, Sabia Kouser, Ranjitha R,
Manasa DJ. Phyto assisted synthesis and characterization of Scoparia dulsis L. leaf extract
mediated porous nano CuO photocatalysts and its anticancer behavior. Applied Nanoscience. 2020;1-20. https://doi.org/10.1007/s13204-020-01536-2.
Mary Joselin J, Ganesh Kumar V, Selvaraj T, Govindaraju K, Karthick V. Biosynthesis of functionalized gold nanoparticles by using methyl commate C in Scoparia dulcis leaf extract as reducing agent. Rasayan J Chem. 2020;13(1):188-94. http://dx.doi.org/10.31788/RJC.2020.1315515
Kalathil Rajan Rakhimol, Sabu Thomas, Nandakumar Kalarikkal, Kochupurakkal Jayachandran, Casein stabilized metal and metal oxide nanoparticles for the efficient in vitro culturing of Scoparia dulcis L., Journal of Siberian Federal University. Biology. 2021;14(4):498-509. https://doi.org/10.17516/1997-1389-0367
Meghana Navada K, Nagaraja GK, Josline Neetha D’Souza, Sabia Kouser, Ravikumar CR Manasa DJ. Bio-fabrication of multifunctional quasi-spherical green ?-Fe2O3 nanostructures for paracetamol sensing and biomedical applications. Ceramics International. 2021;47(23):33651-66. https://doi.org/10.1016/j.ceramint.2021.08.275
Vishnu Sankar Sivasankarapillai, Nishkala Krishnamoorthy, Gaber E. Eldesok Saikh Mohammad Wabaidur, Md Ataul Islam, Ragupathy Dhanusuraman, Vinoth Kumar Ponnusamy. One pot green synthesis of ZnO nanoparticles using Scoparia dulcis plant extract for antimicrobial and antioxidant activities. Applied Nanoscience. 2022;1-11. https://doi.org/10.1007/s13204-022-02610-7
Malik MA, Wani MY, Hashim MA. Nanotoxicity: dimensional and morphological concerns. Advances in Phy Chem. 2011;1-15. https://doi.org/10.1155/2011/450912
Hu YL, Gao JQ. Potential neurotoxicity of nanoparticles. Int J Pharmacol. 2010;394(1-2):115-21. https://doi.org/10.1016/j.ijpharm.2010.04.026
Naveed A, Haq UI, Nadhman A. Synthesis approaches of zinc oxide nanoparticles: the dilemma of ecotoxicity. J Nanomater. 2017;1-14. https://doi.org/10.1155/2017/8510342
Masserini M, Nanoparticles for brain drug delivery. ISRN Biochem. 2013;1-18. http://dx.doi.org/10.1155/2013/238428
Mubo A.S, Ibukun O.A. Medicinal plants used in the treatment of neurodegenerative disorders in some parts of Southwest Nigeria. African J of Pharm Pharmacol. 2015;9(38):956-65. https://doi.org/10.5897/AJPP2014.4164
Ashraf M, Ahmad K, Ahmad I, Ahmad S, Arshad S, Shah SMA, Nasim FUH. Acetyl cholinesterase and NADH oxidase crude enzyme inhibitory activity of some medicinal plants. J Med Plants Res. 2011;5(10):2086-89.
Pragati Jamdagni, Poonam Khatri Rana JS. Green synthesis of zinc oxide nanoparticles using flower extract of Nyctanthesarbortristis and their antifungal activity. J King Saud Univ Sci. 2016;30:168-75. doi:10.1016/j.jksus.2016.10.002
Santhoshkumar J, Venkat Kumar S, Rajeshkumar S. Synthesis of zinc oxide nanoparticles using plant leaf extract against urinary tract infection pathogen. Resource-Efficient Technologies. 2017;3(4):459-65. https://doi.org/10.1016/j.reffit.2017.05.001
Keerthana D.S, Namratha K, Byrappa K, Yathirajan HS, Magn Magn Mater J. 2015;378:551-57. https://doi.org/10.1016/j.jmmm.2014.10.176
Zheleva-Dimitrova D, Nedialkov P, Kitanov G, Radical scavenging and antioxidant activities of methanolic extracts from Hypericum species growing in Bulgaria. Pharmacogn Mag. 2010;6(22):74-78. https://doi.org/10.4103%2F0973-1296.62889
Subramanian R, Subbramaniyan P, Raj V. Antioxidant activities of Peltophorum pterocarpum flower extract. Springer Plus. 2013;2:28-39. https://doi.org/10.1186/2193-1801-2-28
Minha Naseer, Usman Aslam, Bushra Khalid, Bin Chen. Green route to synthesize Zinc Oxide Nanoparticles using leaf extracts of Cassia fstula and Melia azadarach and their antibacterial potential. Scientific Reports. 2020;10:9055. https://doi.org/10.1038/s41598-020-65949-3.
Fatemeh Norouzi Jobie, Mojtaba, Ranjbar, Akbar Hajizadeh Moghaddam, Mahmoud Kiani. Green synthesis of zinc oxide nanoparticles using Amygdalus scoparia Spach stem bark extract and their applications as an alternative antimicrobial, anticancer and anti-diabetic agent. Adv Powder Technol. 2021;32(6):2043-52. https://doi.org/10.1016/j.apt.2021.04.014
Meron Girma Demissie, Fedlu Kedir Sabir, Gemechu Deressa Edossa, Bedasa Abdisa Gonfa. Synthesis of zinc oxide nanoparticles using leaf extract of lippiaadoensis (Koseret) and evaluation of its antibacterial activity. Journal of Chemistry. 2020;1-9. https://doi.org/10.1155/2020/7459042
Stan M, Popa A, Toloman D, Silipas T.D, Vodnar D.C. Antibacterial and antioxidant activities of ZnO nanoparticles synthesized using extracts of Allium sativum, Rosmarinus officinalis and Ocimum basilicum. Acta Met Sin Engl Lett. 2016;29(3):228-36. https://doi.org/10.1007/s40195-016-0380-7
Lanje AS, Sharma SJ, Ningthoujam RS, Ahn JS, Pode RB. Low temperature dielectric studies of zinc oxide (ZnO) nanoparticles prepared by precipitation method. Adv Powder Technol. 2013;24(1):331-35. https://doi.org/10.1016/j.apt.2012.08.005
Senthilkumar N, Nandhakumar E, Priya P, Soni D, Vimalan M, Potheher I.V. Synthesis of ZnO nanoparticles using leaf extract of Tectona grandis (L.) and their anti-bacterial, anti-arthritic, anti-oxidant and in vitro cytotoxicity activities. New J Chem. 2017;41(18):10347-56. https://doi.org/10.1039/C7NJ02664A
Nargis Aman, Khalid Rauf, Shujaat Ali, Khan Ahmed, Tokhi Naeem-Ur, Rehman Muhammad, Arfat Yameen. Effect of commercial and green synthesized ZnO NPs in murine model of chloroquine-induced pruritus. Int J Nanomed. 2019;14:3103-10. https://doi.org/10.2147/ijn.s202256
Bandeira M, Giovanela M, Roesch-Ely M. Green synthesis of zinc oxide nanoparticles: a review of the synthesis methodology and mechanism of formation. Sustain Chem Pharm. 2020;15:100223. https://doi.org/10.1016/j.scp.2020.100223
El-Belely E.F, Farag M.M.S, Said H.A. Green synthesis of zinc oxide nanoparticles (ZnO-NPs) using Arthrospira platensis (Class: Cyanophyceae) and evaluation of their biomedical activities. Nanomaterials. 2021;11:95. https://doi.org/10.3390/nano11010095
Pillai AM, Sivasankarapillai VS, Rahdar A. Green synthesis and characterization of zinc oxide nanoparticles with antibacterial and antifungal activity. J Mol Struct. 2020;1211:128107. https://doi.org/10.1016/j.molstruc.2020.128107
Nandhini Baskaran, Anitha Subash. Green synthesis and characterization of Zinc Oxide nanoparticles from Camellia sinensis leaf extract and its potential antibacterial activity and acetylcholinesterase inhibitory activities. J Pharm Res International. 2021;33(51A):134-47. https://doi.org/10.9734/jpri/2021/v33i51A33477
Akintunde JK, Farai TI, Arogundade MR, Adeleke JT. Biogenic zincoxide nanoparticles of Moringa oleifera leaves abrogates rotenone induced neuroendocrine toxicity by regulation of oxidative stress and acetylcholinesterase activity. Biochem and Biophys reports. 2021;1-10. https://doi.org/10.1016/j.bbrep.2021.100999.
Downloads
Published
Versions
- 01-04-2023 (2)
- 04-02-2023 (1)
How to Cite
Issue
Section
License
Copyright (c) 2022 R Mini, V Prabhu, K Poonkodi, K Vimaladevi, M Anusuya, M Vasuki
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
