Priming-mediated triggering of antioxidative response to induce drought tolerance in Maize (Zea mays L.)

Authors

DOI:

https://doi.org/10.14719/pst.2109

Keywords:

Catalase activity, Drought, Leaf Area Index, Malondialdehyde, Membrane Stability Index, Proline content

Abstract

Drought is a well-known issue in plants and it occurs when plants do not receive enough water to meet their requirements. Hence it alters the metabolic process of the plant, consequently reducing the yield. To overcome the loss of yield under prevailing situations, triggering of the antioxidative defense system is required which can mitigate the impact of drought on plants. The priming chemicals KNO3, Mg(NO3)2 and GA3 were evaluated with hydro-priming to know the mitigative response of priming against drought-induced stress in Maize plants. The morpho-physiological and biochemical parameters were used to evaluate the impact of priming-mediated triggers on the antioxidative response. The results of this work indicate that leaf area index (LAI), crop growth rate (CGR, mg g-1 day-1), total chlorophyll, and chl‘a’ (mg g-1) were recorded maximum in T5 (Mg(NO3)2, 10 mM) while chl ‘b’ in T4 (Mg (NO3)2, 7 mM). The maximum Membrane Stability Index (MSI %) and Membrane Injury Index (MII%) were recorded in T5 and T0 (Control). The osmoregulatory compound proline content (µg g-1) and antioxidative enzyme catalase (nm H2O2 mg-1 min-1) were detected in significantly highest quantity in T3 (KNO3, 15 mM) while the least amount of malondialdehyde (MDA nm g-1) was found under the same treatment. The correlation studies amongst all the parameters reflected that MII % and MDA content (MDA nm g-1) negatively correlated with the remaining parameters studied. This study has reflected that out of all the sources of priming treatments, KNO3 in 15 mM and Mg(NO3)2 in 10 mM has the potential to trigger the antioxidative defense mechanism to mitigate the response of drought in Maize.

Downloads

Download data is not yet available.

References

Boreddy SR, Ganesan KN, Ravikesavan R, Senthil N, Babu R. Genotype-by-environment interaction and yield stability of maize (Zea Mays L.) single cross hybrids. Electron J Plant Breed. 2020;11(1):184-91. https://www.ejplantbreeding.org/index.php/EJPB/article/view/3463

Ranum P, Peña-Rosas JP, Garcia-Casal MN. Global maize production, utilization, and consumption. Ann. N. Y. Acad. Sci. 2014;1312(1): 105-112. https://doi.org/10.1111/nyas.12396

Abid M, Ali S, Qi LK, Zahoor R, Tian Z, Jiang D, Snider JL, Dai T. Physiological and biochemical changes during drought and recovery periods at tillering and jointing stages in wheat (Triticum aestivum L.). Sci Rep. 2018;8(1):1-5. doi: https://doi.org/10.1038/s41598-018-21441-7

Seleiman MF, Al-Suhaibani N, Ali N, Akmal M, Alotaibi M, Refay Y, Dindaroglu T, Abdul-Wajid HH, Battaglia ML. Drought stress impacts on plants and different approaches to alleviate its adverse effects. Plants. 2021;10(2):259. https://doi.org/10.3390/plants10020259

Bayissa Y, Maskey S, Tadesse T, Van Andel SJ, Moges S, Van Griensven A, Solomatine D. Comparison of the performance of six drought indices in characterizing historical drought for the upper Blue Nile basin, Ethiopia. Geosci. 2018; 8(3):81. https://doi.org/10.3390/geosciences8030081

Kandpal G, KumarP, Siddique A. Effect of drought and improvement mechanism in rice: a review. Ann Agri Bio Res. 2018;23(2):150-5. https://www.cabdirect.org/cabdirect/abstract/20193088682

Siddique A, Kandpal G, Kumar P. Proline accumulation and its defensive role under diverse stress condition in plants: An overview. J Pure Appl Microbiol. 2018;12(3):1655-9. https://doi.org/10.22207/JPAM.12.3.73

Singh V, Sharma M, Upadhyay H, Siddique A. Ameliorative effect of seed priming on germination, vigour index and tolerance index against short term moisture stress in maize (Zea mays L.). Indian J Agric Res. 2020;54(3):378-82. https://arccjournals.com/journal/indian-journal-of-agricultural-research/A-5351

Singh A, Banerjee A, Roychoudhury A. Seed priming with calcium compounds abrogate fluoride-induced oxidative stress by upregulating defence pathways in an indica rice variety. Protoplasma. 2020; 257(3):767-82. https://doi.org/10.1007/s00709-019-01460-5

Siddique A, Bose B. Effect of seed invigoration with nitrate salts on morpho-physiological and growth parameters of wheat crop sown in different dates in its cropping season. Vegetos. 2015;28(1):76-85. https://doi.org/10.5958/2229-4473.2015.00011.7

Ali LG, Nulit R, Ibrahim MH, Yien CY. Enhancement of germination and early seedling growth of rice (Oryza sativa) var. FARO44 by seed priming under normal and drought stressed conditions. J plant Nutr. 2020;43(11):1579-93. https://doi.org/10.1080/01904167.2020.1739298

Srivastava AK, Siddique A, Sharma MK, Bose B. Seed priming with salts of nitrate enhances nitrogen use efficiency in rice. Vegetos. 2017;30(4):99-104. https://doi.org/10.5958/2229-4473.2017.00199.9

Marchin RM, Ossola A, Leishman MR, Ellsworth DS. A simple method for simulating drought effects on plants. Front Plant Sci. 2020; 10: 1715. https://doi.org/10.3389/fpls.2019.01715

Watson DJ. Comparative physiological studies on the growth of field crops: I. Variation in net assimilation rate and leaf area between species and varieties, and within and between years. Ann Bot. 1947;11(41):41-76. https://www.jstor.org/stable/42907002

Sairam RK, Srivastava GC, Agarwal S, Meena RC. Differences in antioxidant activity in response to salinity stress in tolerant and susceptible wheat genotypes. Biol Plant. 2005; 49: 85-91. https://link.springer.com/article/10.1007/s10535-005-5091-2

Arnon DI. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant physiol. 1949; 24(1):1.doi: 10.1104/pp.24.1.1

Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for water-stress studies. Plant and soil. 1973; 39(1):205-7. https://link.springer.com/article/10.1007/BF00018060

Heath RL, Packer L. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of biochemistry and biophysics. 1968;125(1):189-98. https://doi.org/10.1016/0003-9861(68)90654-1

Aebi H. Catalase in vitro. In Methods in enzymology 1984 Jan 1 (Vol. 105, pp. 121-126). Academic Press. https://doi.org/10.1016/S0076-6879(84)05016-3

Bodner G, Nakhforoosh A, Kaul HP. Management of crop water under drought: a review. Agronomy for Sustainable Development. 2015;35(2):401-42. https://link.springer.com/article/10.1007/s13593-015-0283-4

Sehgal A, Sita K, Siddique KH, Kumar R, Bhogireddy S, Varshney RK, Hanumantha Rao B, Nair RM, Prasad PV, Nayyar H. Drought or/and heat-stress effects on seed filling in food crops: impacts on functional biochemistry, seed yields, and nutritional quality. Front Plant Sci. 2018;9:1705. https://doi.org/10.3389/fpls.2018.01705

Anjum SA, Ashraf U, Tanveer M, Khan I, Hussain S, Shahzad B, Wang LC. Drought induced changes in growth, osmolyte accumulation and antioxidant metabolism of three maize hybrids. Front Plant sci. 2017;8: 69. https://www.frontiersin.org/articles/10.3389/fpls.2017.00069/full

Anaytullah, Bose B. Nitrate-hardened seeds increase germination, amylase activity and proline content in wheat seedlings at low temperature. Physiol Mol Biol Plants. 2007; 13: 199-207. https://www.researchgate.net/publication/331158051

Singh V, Sharma M, Siddique A. Effect of seed invigoration treatment with nitrate salt on seedling growth of maize (Zea mays L) under short term moisture stress induced by PEG-6000. Indian J Ecol. 2020;47(4): 992-996. http://indianecologicalsociety.com/society/wpcontent/themes/ecology/fullpdfs/1606993007.pdf

Singh V, Siddique A, Krishna V, Singh M. Effect of seed priming treatment with nitrate salt on phytotoxicity and chlorophyll content under short term moisture stress in maize (Zea mays L.). Nat Environ Pollut Technol. 2020;19(3):1119-1123. http://neptjournal.com/upload-images/(23)B-3647.pdf

Anaytullah, Srivastava AK, Bose B. Impact of seed hardening treatment with nitrate salts on nitrogen and anti-oxidant defense metabolisms in wheat (Triticum aestivum L.) under timely, late and very late sown conditions. Vegetos. 2012;25(1): 292-299. https://www.semanticscholar.org/paper/Impact-of-Seed-Hardening-Treatment-with-Nitrate-on-Anaytullah-Srivastava/f471e691d9a8dfd3ad2ea835a17dbde151a268c5

Nieves-Cordones M, García-Sánchez F, Pérez-Pérez JG, Colmenero-Flores JM, Rubio F, Rosales MA. Coping with water shortage: an update on the role of K+, Cl-, and water membrane transport mechanisms on drought resistance. Front Plant Sci. 2019;1619. https://doi.org/10.3389/fpls.2019.01619

Bahrami-Rad S, Hajiboland R. Effect of potassium application in drought-stressed tobacco (Nicotiana rustica L.) plants: Comparison of root with foliar application. Ann Agric Sci. 2017;62(2):121-30. https://doi.org/10.1016/j.aoas.2017.08.001

Chen K, Fessehaie A, Arora R. Aquaporin expression during seed osmopriming and post-priming germination in spinach. Biol Plant. 2013;57(1):193-8. https://link.springer.com/article/10.1007/s10535-012-0266-0

Bose B, Srivastava A K, Siddique A. Impact of nitrate salts hardened seeds and sowing dates on seedling stand, growth, yield attributes, nitrogen and stress metabolism of rice. Int J Agric, Environ Biotechnol. 2016;9(3):1-12. https://www.indianjournals.com/ijor.aspx?target=ijor:ijaeb&volume=9&issue=3&article=008

Singhal RK, Pandey S, Bose B. Seed priming with Mg (NO3)2 and ZnSO4 salts triggers physio-biochemical and antioxidant defense to induce water stress adaptation in wheat (Triticum aestivum L.). Plant Stress. 2021; 2: 100037. https://doi.org/10.1016/j.stress.2021.100037

Tamimi SM. Effect of seed priming on growth and physiological traits of five Jordanian wheat (Triticum aestivum L.) landraces under salt stress. J Biosci Agric Res. 2016; 11(1):906-922. https://www.journalbinet.com/jbar-110116-111.html

Liaqat S, Chhabra S, Saffeullah P, Iqbal N, Siddiqi TO. Role of potassium in drought adaptation: Insights into physiological and biochemical characteristics of plants. In role of potassium in abiotic stress 2022 (pp. 143-162). Springer, Singapore. https://doi.org/10.1007/978-981-16-4461-0_7

Ben Youssef R, Jelali N, Boukari N, Albacete A, Martinez C, Alfocea FP, Abdelly C. The efficiency of different priming agents for improving germination and early seedling growth of local Tunisian barley under salinity stress. Plants. 2021;10(11):2264.https://doi.org/10.3390/plants10112264

Imran KH, Zafar H, Chattha MU, Mahmood A, Maqbool R, Athar F, Alahdal MA, Farhana BI, Mahmood F, Hassan MU. Seed priming with different agents mitigate alkalinity induced oxidative damage and improves maize growth. Not Bot Horti Agrobot Cluj Napoca. 2022;50(1):12615-12615. https://doi.org/10.15835/nbha50112615

Published

19-03-2023 — Updated on 01-04-2023

Versions

How to Cite

1.
Thongbam S, Sinam V, Mentada BAS, Kalangutkar AM, Siddique A. Priming-mediated triggering of antioxidative response to induce drought tolerance in Maize (Zea mays L.). Plant Sci. Today [Internet]. 2023 Apr. 1 [cited 2024 Nov. 21];10(2):247-52. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/2109

Issue

Section

Research Articles

Similar Articles

You may also start an advanced similarity search for this article.