Effect of salicylic acid on cowpea seedlings under saline stress

Diana Jhulia Palheta de Sousa; Tamirys Marcelina da Silva; Marcio Augusto Costa Carmona Junior; Glauco André dos Santos Nogueira; Ana Ecídia de Araújo Brito; Luma Castro de Souza; Cândido Ferreira de Oliveira Neto; Gerson Diego Pamplona Albuquerque

doi:10.14719/pst.2237

Authors

Diana Jhulia Palheta de Sousa ICA, Universidade Federal Rural da Amazônia, Belém 66.077-830, Brasil https://orcid.org/0000-0002-8050-7260
Tamirys Marcelina da Silva ICA, Universidade Federal Rural da Amazônia, Belém 66.077-830, Brasil https://orcid.org/0000-0001-8588-3866
Marcio Augusto Costa Carmona Junior ICA, Universidade Federal Rural da Amazônia, Belém 66.077-830, Brasil https://orcid.org/0000-0002-3471-8016
Glauco André dos Santos Nogueira ICA, Universidade Federal Rural da Amazônia, Belém 66.077-830, Brasil https://orcid.org/0000-0003-3229-5694
Ana Ecídia de Araújo Brito ICA, Universidade Federal Rural da Amazônia, Belém 66.077-830, Brasil https://orcid.org/0000-0002-6927-0346
Luma Castro de Souza ICA, Universidade Federal Rural da Amazônia, Belém 66.077-830, Brasil https://orcid.org/0000-0002-4500-4035
Cândido Ferreira de Oliveira Neto ICA, Universidade Federal Rural da Amazônia, Belém 66.077-830, Brasil https://orcid.org/0000-0002-6070-0549
Gerson Diego Pamplona Albuquerque ICA, Universidade Federal Rural da Amazônia, Belém 66.077-830, Brasil https://orcid.org/0000-0002-7982-8817

DOI:

https://doi.org/10.14719/pst.2237

Keywords:

Aminoacids, Proline, Glycine-bethayne, Salinity, Carbohydrates

Abstract

The aim of this work was applying salicylic acid (SA) in cowpea seedlings under saline stress. The experiment took place in the seed laboratory of the Universidade Federal Rural da Amazônia with a completely randomized experimental design in a 2 x 2 x 3 factorial scheme, with two bean cultivars (Canapu and Pingo-de-ouro), two levels of salicylic acid (0, and 0.50 mM) and three salt stress levels (0, 25, 50 mM). The seeds were previously soaked in salicylic acid (0 and 0.50 mM) for a period of 12 hours and then placed in germitest paper rolls for treatments with NaCl (0, 25, 50 mM) for a period of 12 days at room temperature constant 27 °C. There was a significant effect of cultivars, AS dose and NaCl concentrations and their interactions on most of the analyzed variables. Root and leaf proline concentrations were higher in pingo-de-ouro cultivar, Canapu cultivar had better performance in biomass accumulation. Salicylic acid reduced proteins in the leaves by 13.33%, while in the root there was an increase of 12.61%, ammonium concentrations reduced in the roots by 11.9%. When applied to salinity (25 and 50 mM) there was an increase of proteins in the leaves 40.83% and 27.48% respectively, and a reduction of amino acids of 30.24 and 25.24% in NaCl dosages (25 and 50 mM) respectively. Salinity reduced biomass accumulation and interfered with cellular solute production. However, the application of salicylic acid promoted salt stress tolerance in Canapu cultivar.

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