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Plant Science Today (PST)

Research Articles

Vol. 10 No. 3 (2023)

Identification of bioactive compounds from the ethnomedicinal plant Senna alata (L.) Roxb. (fabaceae) through in vitro and molecular docking analysis against ?-glucosidase enzyme: a diabetic drug target

DOI
https://doi.org/10.14719/pst.2279
Submitted
6 December 2022
Published
20-04-2023 — Updated on 01-07-2023
Versions

Abstract

Senna alata (L.) Roxb. belongs to the family Fabaceae, is reported to have traditional use to treat diabetics and is selected for the study. Preliminary phytochemical analysis was carried out in the selected plant, indicating comparatively higher amounts of phenol, flavonoid, tannin and alkaloids in quantification. The antidiabetic activity of the plant was analyzed and the result indicated that the acetone and methanolic extract showed the lowest IC50 values in a-amylase and a-glucosidase assays respectively. The methanolic extract, which showed an IC50 (39.977 ug/ml) value similar to the standard (35.151 ug/ml), was selected for HR-LCMS analysis. HR-LCMS analysis indicated compounds that exhibit antidiabetic properties, including rutin, kaempferol, rhein and luteolin in the extract. Molecular docking analysis revealed 5 compounds showing better binding affinity namely 5-methoxyhydnocarpin-D, quercetin 3-rhamnoside-7-glucoside, marimetin, kaempferol and luteolin, than the standard drugs voglibose and acarbose. The present in vitro antidiabetic study against 5NN8 target protein was supported by molecular docking analysis. Therefore, further study of bioactive compounds identified through HR-LC MS can help develop future drug leads. Using such medicinal plants can support the improvement of the healthcare system as they do not have many side effects. S. alata is an important medicinal plant, but at the same time, it has become a weed in different parts of Kerala. Validation of medicinal properties and identification of bioactive molecules can help the sustainable utilization of the plant.

References

  1. Ezuruike UF, Prieto JM. The use of plants in the traditional management of diabetes in Nigeria: Pharmacological and toxicological considerations. J Ethnopharmacol [Internet]. 2014;155(2):857-924. Available from: http://dx.doi.org/10.1016/j.jep.2014.05.055
  2. Collado-Mesa F, Barceló A, Arheart KL, Messiah SE. An ecological analysis of childhood-onset type 1 diabetes incidence and prevalence in latin America. Rev Panam Salud Publica/Pan Am J Public Heal. 2004;15(6):388-94. https://doi.org/10.1590/S1020-49892004000600004
  3. Sharma S, Chaturvedi M, Edwin E, Shukla S, Sagrawat H. Evaluation of the phytochemicals and antidiabeticactivity of Ficus bengalensis. Int J Diab Dev Ctries [Internet]. 2007;27(2):56-59. Available from: http://www.ijddc.com; https://doi.org/10.4103/0973-3930.37036
  4. WHO. World Health Organization. World malaria report. 2015. 2016.
  5. Carracher AM, Marathe PH, Close KL. International Diabetes Federation 2017. J Diabetes. 2018;10(5):353-56. https://doi.org/10.1111/1753-0407.12644
  6. Prabhakar PK, Doble M. Mechanism of action of natural products used in the treatment of diabetes mellitus. Chin J Integr Med. 2011;17(8):563-74. https://doi.org/10.1007/s11655-011-0810-3
  7. Roselli M, Lentini G, Habtemariam S. Phytochemical, antioxidant and anti-?-glucosidase activity evaluations of Bergenia cordifolia. Phyther Res. 2012;26(6):908-14. https://doi.org/10.1002/ptr.3655
  8. Zhao C, Yang C, Wai STC, Zhang YP, Portillo M, Paoli P. Regulation of glucose metabolism by bioactive phytochemicals for the management of type 2 diabetes mellitus. Crit Rev Food Sci Nutr [Internet]. 2019;59(6):830-47. Available from: https://doi.org/10.1080/10408398.2018.1501658
  9. Alam S, Sarker MMR, Sultana TN, Chowdhury MNR, Rashid MA, Chaity NI, Mohamed I N. Antidiabetic phytochemicals from medicinal plants: Prospective candidates for new drug discovery and development. Diabetes mellitus and COVID-19: understanding the association in light of current evidence. Front in endocrinol. 2022;9(28):8327-39. https://doi.org/10.3389/fendo.2022.800714
  10. Abatan MO. A note on the anti-inflammatory action of plants of some Cassia species. Fitoterapia. 1990;61(4):336-38.
  11. Oladeji OS, Odelade KA, Oloke JK. Phytochemical screening and antimicrobial investigation of Moringa oleifera leaf extracts. African J Sci Technol Innov Dev [Internet]. 2020;12(1):79-84. Available from: https://doi.org/10.1080/20421338.2019.1589082
  12. Muniyappa R, Gubbi S. COVID-19 pandemic, coronaviruses and diabetes mellitus. Am J Physiol Endocrinol Metab. 2020 Apr 26.
  13. Fatmawati S, Purnomo AS, Bakar MF. Chemical constituents, usage and pharmacological activity of Cassia alata. Heliyon. 2020 Jul 1;6(7). https://doi.org/10.1016/j.heliyon.2020.e04396
  14. Oladeji OS, Odelade KA, Oloke JK. Ethnobotanical description and biological activities of Senna alata. J Evid Based Complementary Altern Med. 2020;12(1):79-84. Available from; https://doi.org/10.1080/20421338.2019.1589082
  15. Gul R, Jan SU, Faridullah S, Sherani S, Jahan N. Preliminary phytochemical screening, quantitative analysis of alkaloids and antioxidant activity of crude plant extracts from Ephedra intermedia indigenous to Balochistan. Sci World J. 2017.https://doi.org/10.1155/2017/5873648
  16. Hiai S, Oura HN. Color reaction of some sapogenins with vanillin sulphuric acid. Planta Med. 1976;29(2):116-22. https://doi.org/10.1055/s-0028-1097639
  17. Lister E, Wilson P. Measurement of total phenolics and ABTS assay for antioxidant activity (personal communication). Crop Research Institute, Lincoln, New Zealand. 2001;7:235-39.
  18. Chang CV, Felício AC, Reis JE de P, Guerra MDO, Peters VM. Fetal toxicity of Solanum lycocarpum (Solanaceae) in rats. J Ethnopharmacol. 2002;81(2):265-69. https://doi.org/10.1016/S0378-8741(02)00092-2
  19. Mukhopadhyay N, Sarkar S, Bandyopadhyay S. Effect of extrusion cooking on anti-nutritional factor tannin in linseed (Linum usitatissimum) meal. Int J Food Sci Nutr. 2007;58(8):588-94. https://doi.org/10.1080/09637480701343952
  20. Kim YM, Jeong YK, Wang MH, Lee WY, Rhee HI. Inhibitory effect of pine extract on ?-glucosidase activity and postprandial hyperglycemia. Nutrition. 2005;21(6):756-61. https://doi.org/10.1016/j.nut.2004.10.014
  21. Kwon YII, Vattem DA, Shetty K. Evaluation of clonal herbs of Lamiaceae species for management of diabetes and hypertension. Asia Pac J Clin Nutr. 2006;15(1):107-18.
  22. Kumbhar ST, Patil SP, Une HD. Phytochemical analysis of Canna indica L. roots and rhizomes extract. Biochem Biophys Reports [Internet]. 2018;16(September):50-55. Available from: https://doi.org/10.1016/j.bbrep.2018.09.002
  23. Junejo JA, Zaman K, Rudrapal M, Celik I, Attah EI. Antidiabetic bioactive compounds from Tetrastigma angustifolia (Roxb.) Deb and Oxalis debilis Kunth.: Validation of ethnomedicinal claim by in vitro and in silico studies. South African J Bot. 2021;143:164-75. https://doi.org/10.1016/j.sajb.2021.07.023
  24. Fatiha B, Khodir M, Farid D, Tiziri R, Karima B, Sonia O et al. Optimisation of solvent extraction of antioxidants ( phenolic compounds ) from Algerian Mint ( Mentha spicata L .). Pharmacogn Commun. 2012;2(4):72-86. http://hdl.handle.net/123456789/27
  25. Altemimi A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot DA. Phytochemicals: Extraction, isolation and identification of bioactive compounds from plant extracts. Plants. 2017;6(4). https://doi.org/10.3390/plants6040042
  26. Mujeeb F, Bajpai P, Pathak N. Phytochemical evaluation, antimicrobial activity and determination of bioactive components from leaves of Aegle marmelos. Biomed Res Int. 2014. https://doi.org/10.1155/2014/497606
  27. Vinayagam R, Xu B. Antidiabetic properties of dietary flavonoids: A cellular mechanism review. Nutr Metab [Internet]. 2015;12(1):1-20. Available from: http://dx.doi.org/10.1186/s12986-015-0057-7
  28. Vertommen J, van den Enden M, Simoens L, de Leeuw I. Flavonoid treatment reduces glycation and lipid peroxidation in experimental diabetic rats. Phyther Res. 1994;8(7):43032. https://doi.org/10.1002/ptr.2650080711
  29. Panda S, Kar A. Apigenin (4‘,5,7-trihydroxyflavone) regulates hyperglycaemia, thyroid dysfunction and lipid peroxidation in alloxan-induced diabetic mice. J Pharm Pharmacol. 2010;59(11):1543-48. https://doi.org/10.1211/jpp.59.11.0012
  30. Ghorbani A. Mechanisms of antidiabetic effects of flavonoid rutin. Biomed Pharmacother. 2017;96 (September):305-12. https://doi.org/10.1016/j.biopha.2017.10.001
  31. Ola MS, Ahmed MM, Ahmad R, Abuohashish HM, Al-Rejaie SS, Alhomida AS. Neuroprotective effects of rutin in streptozotocin-induced diabetic rat retina. J Mol Neurosci. 2015;56(2):440-48. https://doi.org/10.1007/s12031-015-0561-2
  32. Alam MM, Meerza D, Naseem I. Protective effect of quercetin on hyperglycemia, oxidative stress and DNA damage in alloxan induced type 2 diabetic mice. Life Sci [Internet]. 2014;109(1):8-14. Available from: http://dx.doi.org/10.1016/j.lfs.2014.06.005
  33. Habtemariam S. ?-Glucosidase inhibitory activity of kaempferol-3-O-rutinoside. Nat Prod Commun. 2011;6(2):201-03. https://doi.org/10.1177/1934578X1100600211
  34. Zang Y, Igarashi K, Li YL. Anti-diabetic effects of luteolin and luteolin-7-O-glucoside on KK-Ay mice. Biosci Biotechnol Biochem. 2016;80(8):1580-86. https://doi.org/10.1080/09168451.2015.1116928
  35. Zhou YX, Xia W, Yue W, Peng C, Rahman K, Zhang H. Rhein: A review of pharmacological activities. Evidence-based Complement Altern Med. 2015. https://doi.org/10.1155/2015/578107
  36. Rajah RV. Antioxidant and antihyperglycemic activities of Aquilaria sinensis leaves (Gaharu). Doctoral dissertation, University of Malaya. 2018.
  37. Hermans MMP, Kroos MA, Van Beeumen J, Oostra BA, Reuser AJJ. Human lysosomal ?-glucosidase: Characterization of the catalytic site. J Biol Chem. 1991;266(21):13507-12. https://doi.org/10.1016/S0021-9258(18)92727-4
  38. Thodi RC, Ibrahim JM, Surendran VA, Nair AS, Sukumaran ST. Rutaretin1?-(6? sinapoylglucoside): promising inhibitor of COVID 19 mpro catalytic dyad from the leaves of Pittosporum dasycaulon miq (Pittosporaceae). J Biomol Struct Dyn [Internet]. 2021;0(0):1-17. Available from; https://doi.org/10.1080/07391102.2021.1972841
  39. Shah B, Modi P, Sagar SR. In silico studies on therapeutic agents for COVID-19: Drug repurposing approach. Life Sci [Internet]. 2020;252(April):117652. Available from: https://doi.org/10.1016/j.lfs.2020.117652
  40. Moheb M, Iraji A, Dastyafteh N, Khalili Ghomi M, Noori M, Mojtabavi S et al. Synthesis and bioactivities evaluation of quinazolin-4(3H)-one derivatives as ?-glucosidase inhibitors. BMC Chem [Internet]. 2022;16(1):1-12. Available from; https://doi.org/10.1186/s13065-022-00885-z

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