Evaluation of Phenolic Content, Anti-oxidant and modulation of blood indices of Pavetta crassipes
DOI:
https://doi.org/10.14719/pst.2453Keywords:
Immunomodulatory, Antioxidants, Phenolics, Phytochemical evaluationAbstract
Introduction
Pavetta crassipes is a medicinal plant with various documented biological activities. It has a folkloric use as an anti-viral therapy and pharmacologically documented anti-microbial activity. However, its immune modulatory property has not been explored. The study is designed to evaluate the phytochemical profile as well as the anti-oxidant and immunomodulatory potential of the methanol extract of Pavetta crassipes leaf.
Methodology
In vitro antioxidants activity was determined by nitric oxide inhibitory assay. phenolic and flavonoid content of the extract was also determined. The immunomodulatory activity was evaluated using cyclophosphamide induced immunomodulation model in mice. Phytochemical evaluation of leaf extract was done using HPLC analyses. Results were expressed as the mean ± SEM, IC50 value for the antioxidant activity was determined, One-way ANOVA was used for differences in mean followed by Dunnett’s test for multiple comparison for the immunomodulatory activity. To test for level of significance, P< 0.05 was considered significant.
Results
The extract had phenolic and flavonoid contents of 17.6 ±0.1 mg/GAE/g and 8.4 ± 0.3 mg/QE/g respectively. The extract also showed nitric oxide inhibitory antioxidant activity with IC50 of 2.3± 0.4 while gallic and ascorbic acid had IC50 of 1.6 ±0.1 and 8.0 ±0.6 respectively. P. crassipes leaf extract indicated a significant (p<0.05) countering influence to cyclophosphamide induced decrease in total white blood count, differential leucocyte counts and red blood count. HPLC analysis indicated the presence of phenolic compounds such a rutin, quercetin and chlorogenic acid.
Conclusion
The phytochemical profile, anti-oxidant and immunomodulatory properties of the methanol extract of P. crassipes leaf as evaluated in this study corroborates and justifies its folkloric use.
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Copyright (c) 2022 Kevwe Benefit Esievo, Ibrahim Babatunde Gegele, Adamu Aliyu, Solomon Ameh Fidelis, Omolola Temitope Fatokun, Jemilat Aliyu Ibrahim
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