Engineering Drought Tolerance in Crops Using CRISPR Cas systems
DOI:
https://doi.org/10.14719/pst.2524Keywords:
CRISPR/Cas9, drought, DNA, endonuclease, gene, genome editing, sgRNAAbstract
Drought stress is one of the most considerable threats to global agricultural food security, causing yield losses worldwide. Therefore, the search for effective genetic and molecular methods for developing cultivars that are tolerant or resistant to harsh environments has been more intensive over the last decades. Apart from time-consuming conventional breeding techniques, biotechnologists are now investigating modern genome editing tools for engineering tolerance and resistance to various biotic and abiotic stresses in crops. Various genetic engineering techniques such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) were developed based on the discovery of the DNA structure. However, these methods have limitations, with ZFNs being prone to errors due to their limited base pair recognition, and TALENs requiring a complex protein engineering process and struggling to cleave methylated DNA. In recent years, clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) and its alternatives have gained popularity in plant biotechnology. Out of the genome editing techniques mentioned earlier, CRISPR/Cas9 is becoming more popular because it's faster and easier to use. Given that drought is now a significant threat to global agriculture due to the drying of arable lands, this review focuses on how we can use CRISPR genome editing to enhance crop tolerance to drought stress and explores its future potential.
Downloads
References
Orimoloye IR, Belle JA, Orimoloye YM, Olusola AO, Ololade OO. Drought: A Common Environmental Disaster. Atmosphere. 2022;13(1):111. https://doi.org/10.3390/atmos13010111.
Mansoor S, Khan T, Farooq I, Shah LR, Sharma V, Sonne C, Rinklebe J, Ahmad P. Drought and global hunger: biotechnological interventions in sustainability and management. Planta. 2022;256(5):97. https://doi.org/10.1007/s00425-022-04006-x.
Antwi-Agyei P, Fraser EDG, Dougill AJ, Stringer LC, Simelton E. Mapping the vulnerability of crop production to drought in Ghana using rainfall, yield, and socioeconomic data. Appl Geogr. 2012;32(2):324-334. https://doi.org/10.1016/j.apgeog.2011.06.010
Villalobos-López MA, Arroyo-Becerra A, Quintero-Jiménez A, Iturriaga G. Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops. Int J Mol Sci. 2022;23(19):12053. https://doi.org/10.3390/ijms231912053.
Seleiman MF, Al-Suhaibani N, Ali N, Akmal M, Alotaibi M, Refay Y, Dindaroglu T, Abdul-Wajid HH, Battaglia ML. Drought Stress Impacts on Plants and Different Approaches to Alleviate its Adverse Effects. Plants (Basel). 2021;10(2):259. https://doi.org/10.3390/plants10020259.
Joshi RK, Bharat SS, Mishra R. Engineering drought tolerance in plants through CRISPR/Cas genome editing. 3 Biotech. 2020;10(9):400. https://doi.org/10.1007/s13205-020-02390-3.
Zhou J, Luan X, Liu Y, Wang L, Wang J, Yang S, Liu S, Zhang J, Liu H, Yao D. Strategies and Methods for Improving the Efficiency of CRISPR/Cas9 Gene Editing in Plant Molecular Breeding. Plants. 2023;12(7):1478. https://doi.org/10.3390/plants12071478.
Marraffini LA, Sontheimer EJ. CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea. Nat Rev Genet. 2010;11(3):181-190. https://doi.org/10.1038/nrg2749
Gajardo HA, Gómez-Espinoza O, Boscariol Ferreira P, Carrer H, Bravo LA. The Potential of CRISPR/Cas Technology to Enhance Crop Performance on Adverse Soil Conditions. Plants (Basel). 2023;12(9):1892. https://doi.org/10.3390/plants12091892.
Liu Q, Yang F, Zhang J, Liu H, Rahman S, Islam S, Ma W, She M. Application of CRISPR/Cas9 in Crop Quality Improvement. Int J Mol Sci. 2021;22(8):4206. https://doi.org/ 10.3390/ijms22084206
Deng B, Xue J. HIV infection detection using CRISPR/Cas systems: Present and future prospects. Comput Struct Biotechnol J. 2023;21:4409-4423. https://doi.org/10.1016/j.csbj.2023.09.005.
Dong J, Wu X, Hu Q, Sun C, Li J, Song P, Su Y, Zhou L. An immobilization-free electrochemical biosensor based on CRISPR/Cas13a and FAM-RNA-MB for simultaneous detection of multiple pathogens. Biosens Bioelectron. 2023;241:115673. https://doi.org/10.1016/j.bios.2023.115673.
Yu S, Zhao R, Zhang B, Lai C, Li L, Shen J, Tan X, Shao J. Research progress and application of the CRISPR/Cas9 gene-editing technology based on hepatocellular carcinoma. Asian J Pharm Sci. 2023;18(4):100828. https://doi.org/10.1016/j.ajps.2023.100828.
Ning L, Xi J, Zi Y, Chen M, Zou Q, Zhou X, Tang C. Prospects and challenges of CRISPR/Cas9 gene-editing technology in cancer research. Clin Genet. 2023 Sep 14. https://doi.org/10.1111/cge.14424.
Shan Q, Wang Y, Li J, et al. Targeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol. 2013;31(8):686-688. https://doi.org/10.1038/nbt.2650
Sami A, Xue Z, Tazein S, Arshad A, He Zhu Z, Ping Chen Y, Hong Y, Tian Zhu X, Jin Zhou K. CRISPR-Cas9-based genetic engineering for crop improvement under drought stress. Bioengineered. 2021;12(1):5814-5829. https://doi.org/10.1080/21655979.2021.1969831
Jaganathan D, Ramasamy K, Sellamuthu G, Jayabalan S, Venkataraman G. CRISPR for Crop Improvement: An Update Review. Front Plant Sci. 2018;9:985. https://doi.org/10.3389/fpls.2018.00985
Guo Y, Zhao G, Gao X, Zhang L, Zhang Y, Cai X, Yuan X, Guo X. CRISPR/Cas9 gene editing technology: a precise and efficient tool for crop quality improvement. Planta. 2023;258(2):36. https://doi.org/10.1007/s00425-023-04187-z.
Osakabe Y, Watanabe T, Sugano SS, Ueta R, Ishihara R, Shinozaki K, Osakabe K. Optimization of CRISPR/Cas9 genome editing to modify abiotic stress responses in plants. Sci Rep. 2016;6:26685. https://doi.org/10.1038/srep26685
Fang Y, Xiong L. General mechanisms of drought response and their application in drought resistance improvement in plants. Cell Mol Life Sci. 2015;72:673-689. https://doi.org/10.1007/s00018-014-1767-0.
Hsu PK, Dubeaux G, Takahashi Y, Schroeder JI. Signaling mechanisms in abscisic acid-mediated stomatal closure. Plant J. 2021;105(2):307-321. https://doi.org/10.1111/tpj.15067.
Zhu JK. Salt and drought stress signal transduction in plants. Ann Rev Plant Biol. 2002;53:247-273. doi: 10.1146/annurev.arplant.53.091401.143329
Zhu JK. Abiotic stress signaling and responses in plants. Cell. 2016;167:313-324. https://doi.org/10.1016/j.cell.2016.08.029.
Yoshida T, Fernie AR. Hormonal regulation of plant primary metabolism under drought. J Exp Bot. 2023:erad358. https://doi.org/10.1093/jxb/erad358.
Ogata T, Ishizaki T, Fujita M, Fujita Y. CRISPR/Cas9-targeted mutagenesis of OsERA1 confers enhanced responses to abscisic acid and drought stress and increased primary root growth under nonstressed conditions in rice. PLoS One. 2020;15(12). https://doi.org/10.1371/journal.pone.0243376
Dey A, Samanta MK, Gayen S, Maiti MK. The sucrose non-fermenting 1-related kinase 2 gene SAPK9 improves drought tolerance and grain yield in rice by modulating cellular osmotic potential, stomatal closure, and stress-responsive gene expression. BMC Plant Biol. 2016;16(1):158. https://doi.org/10.1186/s12870-016-0845-x
Lou D, Wang H, Liang G, Yu D. OsSAPK2 Confers Abscisic Acid Sensitivity and Tolerance to Drought Stress in Rice. Front Plant Sci. 2017;8:993. https://doi.org/10.3389/fpls.2017.00993
Chilcoat D, Liu ZB, Sander J. Use of CRISPR/Cas9 for Crop Improvement in Maize and Soybean. Prog Mol Biol Transl Sci . 2017;149:27-46. https://doi.org/10.1016/bs.pmbts.2017.04.005.
Shi J, Gao H, Wang H, Lafitte HR, Archibald RL, Yang M, Hakimi SM, Mo H, Habben JE. ARGOS8 variants generated by CRISPR-Cas9 improve maize grain yield under field drought stress conditions. Plant Biotechnol J. 2017;15(2):207-216. https://doi.org/10.1111/pbi.12603.
Raza A, Charagh S, Razzaq A, et al. Brassicaceae plants response and tolerance to drought stress: physiological and molecular interventions. In: The plant family Brassicaceae. Singapore: Springer; 2020. p. 229–261. https://doi.org/10.1007/978-981-15-6345-4_7.
Kim D, Alptekin B, Budak H. CRISPR/Cas9 genome editing in wheat. Funct Integr Genomics. 2018;18(1):31-41. https://doi.org/10.1007/s10142-017-0572-x.
Nakashima K, Yamaguchi-Shinozaki K, Shinozaki K. The transcriptional regulatory network in the drought response and its crosstalk in abiotic stress responses including drought, cold, and heat. Front Plant Sci. 2014;5:170. https://doi.org/10.3389/fpls.2014.00170.
Morran S, Eini O, Pyvovarenko T, et al. Improvement of stress tolerance of wheat and barley by modulation of expression of DREB/CBF factors. Plant Biotechnol J. 2011;9:230–249. https://doi.org/10.1111/j.1467-7652.2010.00547.x.
Li R, Liu C, Zhao R, Wang L, Chen L, Yu W, Zhang S, Sheng J, Shen L. CRISPR/Cas9-Mediated SlNPR1 mutagenesis reduces tomato plant drought tolerance. BMC Plant Biol. 2019;19(1):38. https://doi.org/10.1186/s12870-018-1627-4
Liu L, Zhang J, Xu J, Li Y, Guo L, Wang Z, Zhang X, Zhao B, Guo YD, Zhang N. CRISPR/Cas9 targeted mutagenesis of SlLBD40, a lateral organ boundaries domain transcription factor, enhances drought tolerance in tomato. Plant Sci. 2020;301:110683. https://doi.org/10.1016/j.plantsci.2020.110683
Chen M, Zhu X, Liu X, Wu C, Yu C, Hu G, Chen L, Chen R, Bouzayen M, Zouine M, Hao Y. Knockout of Auxin Response Factor SlARF4 Improves Tomato Resistance to Water Deficit. Int J Mol Sci. 2021;22(7):3347. https://doi.org/10.3390/ijms22073347
Liao S, Qin X, Luo L, Han Y, Wang X, Usman B, et al. CRISPR/Cas9-Induced Mutagenesis of Semi-Rolled Leaf1,2 Confers Curled Leaf Phenotype and Drought Tolerance by Influencing Protein Expression Patterns and ROS Scavenging in Rice (Oryza sativa L.). Agronomy 2019, 9(11), 728; https://doi.org/10.3390/agronomy9110728
Santosh Kumar VV, Verma RK, Yadav SK, Yadav P, Watts A, Rao MV, Chinnusamy V. CRISPR-Cas9 mediated genome editing of drought and salt tolerance (OsDST) gene in indica mega rice cultivar MTU1010. Physiol Mol Biol Plants. 2020;26(6):1099-1110. doi: 10.1007/s12298-020-00819-w.
Downloads
Published
Versions
- 02-11-2023 (2)
- 29-10-2023 (1)
How to Cite
Issue
Section
License
Copyright (c) 2022 K. Kamalova Lola, Mirzakhmedov Mukhammadjon, Ayubov Mirzakamol , Yusupov Abdurakhmon, Mamajonov Bekhzod, Obidov Nurdinjon, Bashirkhonov Ziyodullo, Murodov Anvarjon, Buriev Zabardast, Abdurakhmonov Ibrokhim
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
