Evaluation of the Antimicrobial and Cytotoxic Activity of Cultivated Valeriana officinalis:

Zinat  Mohammadi; Leila  Pishkar; Zohre Eftekhari; Giti  Barzin; Laleh  Babaeekhou

doi:10.14719/pst.2593

Authors

Zinat Mohammadi Department of Biology, Science and Research Islamshahr Branch, Islamic Azad University, Tehran, Iran; Postal Code: 1477893855 https://orcid.org/0000-0002-1933-5148
Leila Pishkar Department of Biology, Science and Research Islamshahr Branch, Islamic Azad University, Tehran, Iran; Postal Code: 1477893855 https://orcid.org/0000-0002-1428-1515
Zohre Eftekhari Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran; Postal Code: 3159915111 https://orcid.org/0000-0003-3502-9260
Giti Barzin Department of Biology, Science and Research Islamshahr Branch, Islamic Azad University, Tehran, Iran; Postal Code: 1477893855 https://orcid.org/0000-0001-7264-5017
Laleh Babaeekhou Department of Biology, Science and Research Islamshahr Branch, Islamic Azad University, Tehran, Iran; Postal Code: 1477893855 https://orcid.org/0000-0001-8711-8797

DOI:

https://doi.org/10.14719/pst.2593

Keywords:

Antibacterial, Cancer, Extract, Medicinal plants

Abstract

Drug resistance refers to the reduction in the effectiveness of a drug in treating a disease or improving the stability of symptoms. It can occur in various types of pathogens, including bacteria, parasites, viruses, fungi, and cancer cells. This experimental study was conducted between 2018 and 2019 in an area with an annual mean rainfall of 130mm. The sowing date was September 10th, and 2-3 seeds were planted per cell. MTT assays (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) were used to determine the percentage of viability in adenocarcinomic human alveolar basal epithelial cells (A549) and Medical Research Council cell strain 5 (MRC5) cell lines incubated with methanolic extract and valerenic acid for 48 hr. The methanol extract was prepared by adding 1000 mg of rhizomes to 100 mL of methanol, followed by sonication for 30 minutes, stirring, and centrifugation at 4000 rpm for 10 minutes. Minimum inhibitory concentration (MIC) and agar gel diffusion were used to assess the antimicrobial activity of the methanol extract of valerian against two important pathogenic microorganisms, Staphylococcus aureus and Candida albicans. However, valerenic acid did not reveal antimicrobial activity at doses of 200, 100, 50, 25, 12.5, and 6.25 µg/mL. The methanolic extract of V. officinalis contains high quantities of sesquiterpenes, specifically valerenic acid, which did not show cytotoxic effects on A549 and MRC5 cell lines as assessed by the MTT assay. In vivo evaluation of the extract in mice and guinea pigs did not reveal any toxic effects based on histopathological and clinical symptom assessments. Our study confirms that Valeriana officinalis has dose-dependent potential to improve existing treatment approaches for Staphylococcus aureus and Candida albicans infections.

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References

Aedh AI, Al-Swedan AD, Mohammed AA, Alwadai BM, Alyami AY, Alsaaed EA, Almurdhimah NM, Zaki MS, Othman AE, Hasan A. Occurrence of multidrug-resistant strains of Acinetobacter spp.: An emerging threat for nosocomial-borne infection in Najran region, KSA. Trop Med Infect Dis. 2023 Feb 9;8(2):108.https://doi.org/10.3390/tropicalmed8020108

Baniesmaeeili SY, Akbarzadeh A, Riazi G, Abdollahi F, Niromand M. Effects of dietary Moringa oleifera leaf powder and ethanolic extract on expression function of immune genes of Litopenaeus vannamei. Aquatic Animals Nutr. 2023;9(1):27-41. doi: 10.22124/janb.2023.24268.1201

Dastyar N, Lysiuk R. Identification of the most important medicinal plants used for wound healing: An ethnobotanical study of Sistan and Baluchestan province, Southeastern Iran. J Biochem Phytomed. 2023;2(1):16-19. doi: 10.34172/jbp.2023.4.

Wi?niewski J, Szczepanik M, Ko?odziej B, Król B. Plantation methods effects on common valerian (Valeriana officinalis) yield and quality. J Anim Plant Sci. 2016;26(1):177-84.

Yeo YS, Nybo SE, Chittiboyina AG, Weerasooriya AD, Wang YH, Góngora-Castillo E et al. Functional identification of valerena-1, 10-diene synthase, a terpene synthase catalyzing a unique chemical cascade in the biosynthesis of biologically active sesquiterpenes in Valeriana officinalis. J Biol Chem. 2013;288(5):3163-73. https://doi.org/10.1074/jbc.M112.415836

Masyita A, Mustika Sari R, Dwi Astuti A, Yasir B, Rahma Rumata N, Emran T Bin et al. Terpenes and terpenoids as main bioactive compounds of essential oils, their roles in human health and potential application as natural food preservatives. Food Chem X. 2022 Mar;13:100217. https://doi.org/10.1016/j.fochx.2022.100217

Pato?ka J, Jakl J. Biomedically relevant chemical constituents of Valeriana officinalis. J Appl Biomed. 2010;8(1):11-18. https://doi.org/10.2478/v10136-009-0002-z

Morteza E, Akbari GA, Sanavi S, Farahani HA. Determination of the vegetative and reproductive characteristics of valerian (Valeriana officinalis L.) under sowing dates and planting densities at Iran. J Med Plants Res. 2010;4(10):857-61.

Kobus Z. Dry matter extraction from valerian roots (Valeriana officinalis L.) with the help of pulsed acoustic field. Int Agrophysics. 2008 Jan 1;22.

Adaszy?ska-Skwirzy?ska M, Swarcewicz M, Dobrowolska A. The potential of use lavender from vegetable waste as effective anti-bacterial and sedative agents. Med Chem. 2014;4(11):734-37. https://doi.org/10.4172/2161-0444.1000222

Badiee P, Alborzi A. Susceptibility of clinical Candida species isolates to anti-fungal agents by E-test, Southern Iran: A five year study. Iran J Microbiol. 2011 Dec;3(4):183-88.

Fatima A, Naqvi SB, Khaliq SA, Perveen S, Jabeen S. Anti-microbial susceptibility pattern of clinical isolates of Pseudomonas aeruginosa isolated from patients of lower respiratory tract infections. Springerplus. 2012 Dec;1(1):70. https://doi.org/10.1186/2193-1801-1-70

Pharmacopoeia B. British Pharmacopoeia Commission: London. UK; 2017.

Morteza E, Akbari GA, Sanavi S, Foghi B, Abdoli M, Farahani HA. Planting density influence on variation of the essential oil content and compositions in valerian (Valeriana officinalis L.) under different sowing dates. Afr J Microbiol Res. 2009;3(12):897-902.

Ghafari S, Esmaeili S, Aref H, Naghibi F, Mosaddegh M. Qualitative and quantitative analysis of some brands of valerian pharmaceutical products. Stud Ethno-Medicine. 2009;3(1):61-64. https://doi.org/10.1080/09735070.2009.11886339

González ML, Joray MB, Laiolo J, Crespo MI, Palacios SM, Ruiz GM et al. Cytotoxic activity of extracts from plants of Central Argentina on sensitive and multidrug-resistant leukemia cells: Isolation of an active principle from Gaillardia megapotamica. Evid Based Complement Alternat Med. 2018;2018:9185935. https://doi.org/10.1155/2018/9185935

Khademian R, Karimzadeh F, Moradi P, Asghari B. Anti-microbial properties of Valeriana officinalis, Satureja bachtiarica and Thymus daenensismethanolic extracts against Helicobacter pylori. J Pharm Res Int. 2019;26:1-7. https://doi.org/10.9734/jpri/2019/v26i630153

Rondón M, Velasco J, Rojas J, Gámez L, León G, Entralgo E et al. Anti-microbial activity of four Valeriana (Caprifoliaceae) species endemic to the Venezuelan Andes. Rev Biol Trop. 2018;66(3):1282-89. https://doi.org/10.15517/rbt.v66i3.30699

Udgire MS, Pathade GR. Evaluation of anti-microbial activities and phytochemical constituents of extracts of Valeriana wallichii. Asian J Plant Sci Res. 2011;

Shi F, Li Y, Han R, Fu A, Wang R, Nusbaum O, Qin Q, Chen X, Hou L, Zhu Y. Valerian and valeric acid inhibit growth of breast cancer cells possibly by mediating epigenetic modifications. Sci Rep. 2021 Jan 28;11(1):1-10.https://doi.org/10.1038/s41598-021-81620-x

de Oliveria DM, Barreto G, De Andrade DV, Saraceno E, Aon-Bertolino L, Capani F et al. Cytoprotective effect of Valeriana officinalis extract on an in vitro experimental model of Parkinson disease. Neurochem Res. 2009;34:215-20.https://doi.org/10.1007/s11064-008-9749-y

Murtaza BN, tul Muntaha S, Siraj M, Rehman MU, Rehman A. Embryotoxic effects of ciprofloxacin in chicken: A potential risk for antibiotic resistance. Adv in Life Sci. 2023;21;9(4):380-83.

Kazempour M, Shahangian SS, Sariri R. Dracocephalum kotschyi: Inhibition of critical enzyme relevant to type-2 diabetes, essential oil composition, bactericidal and anti-oxidant activity. Caspian J Environ Sci. 2023;1-15. doi: 10.22124/cjes.2023.6256

Shahsavari S, Sarkar S, Sen DJ, Mandal SK. Determination of total anti-oxidant activity of methanolic extract of Falcaria vulgaris. J Biochem Phytomed. 2022;1(1):8-12. doi: 10.34172/jbp.2022.3.

Rahman A, Rauf A, Ali B, Ullah M, Ali M, Ahmad S, Ullah F, Iqbal H, Iqbal M, Ahmad MA. Phytochemical analysis and anti-bacterial activity of Berberis vulgarisextract. Adv Life Sci. 2022 Nov 4;9(3):289-94.

Suhad H, Neihaya H, Raghad A. Evaluating the biological activities of biosynthesized ZnO nanoparticles using Escherichia coli. Caspian J Environ Sci. 2021;19(5):809-15. doi: 10.22124/cjes.2021.5221

Fazeli-Nasab B, Shahraki-Mojahed L, Dahmardeh N. Evaluation of anti-microbial activity of essential oil and ethanolic extract of 10 medicinal plants on Rathayibacter tritici and Xanthomonas translucens. Plant Biotechnol Persa. 2022;4(1):10-17. doi: 10.52547/pbp.4.1.3

Seratnahaei M, Eshraghi SS, Pakzad P, Zahraei-Ramazani A, Yaseri M. Anti-bacterial effects of bioactive metabolites extracted from Nocardia pseudobrasiliensis. Caspian J Environ Sci. 2022;1-10. doi: 10.22124/cjes.2022.6064

Baharvand-Ahmadi B, Bahmani M, Naghdi N, Saki K, Baharvand A, Rafieian-Kopaei M. Medicinal plants used to treat infectious and non-infectious diseases of skin and skin appendages in city of Urmia, Northwest Iran. Der Pharmacia Lettre. 2015;7(11):189-96.

Riani F, Rakhmiati R, Dini Kertasari V, Puji Handayani E, Zuyasna Z, P W Yanuastri. Secondary metabolite compounds of Moringa oleifera leaves in two different urban altitude locations, Indonesia. Caspian J Environ Sci. 2023;1-7. doi: 10.22124/cjes.2023.6414

Bazari Moghaddam S, Bagherzadeh Lakani F, Jalilpour J, Masoumzadeh M, Shenavar Masouleh A. Effects of Echinacea purpurea and Allium sativum powdered extracts on growth indices, survival rate and liver enzymes in farmed Huso huso. Aquatic Animals Nutrition. 2022;8(1):25-39. doi: 10.22124/janb.2023.21942.1164

Dehbandi N, Izadi Amoli R, Oskoueiyan R, GholamiA. The prevalence of vanA gene in clinical isolates of vancomycin-resistant Staphylococcus aureus in a hospital in Mazandaran, Iran. Caspian J Environ Sci. 2019;17(4):319-25. doi: 10.22124/cjes.2019.3805

Kakeshian S, Shirali S, Savari A, Najafzadehvarzi H, Zakeri M. Alterations in histological structure, blood estrogen and progesterone levels after oral administration of garlic extract in yellowfin seabream (Acanthopagrus latus). Aquatic Animals Nutr. 2022;8(2):1-10. doi: 10.22124/janb.2023.23692.1182

Stryjewski ME, Corey GR. Methicillin-resistant Staphylococcus aureus: An evolving pathogen. Clin Infect Dis. 2014;58(suppl_1):S10-19.https://doi.org/10.1093/cid/cit613