Citrus for wellness: Exploring the bioactive properties of Citrus medica fruit peel with emphasis on its anticancer, antioxidant, antimicrobial and anthelmintic properties

Authors

DOI:

https://doi.org/10.14719/pst.2612

Keywords:

Anthelmintic, antibacterial, antioxidant, anticancer, FaDu cell line, hypopharyngeal tumour, Citrus medica

Abstract

Citrus medica (Citron) is an underutilised plant consisting of various bioactive elements with numerous medicinal benefits. The present study aimed to evaluate the bioactive properties, including anthelmintic, antimicrobial, antioxidant and anticancer activities, of chloroform extract obtained from the of fruit peel of C. medica. The different types of phytochemicals present in the chloroform extract were analysed using GC-MS. The major components detected included n-hexadecanoic acid, octadecanoic acid, t-tetradecenal, 1-nonadecene etc. Anthelmintic study was conducted using Eisenia fetida as a test organism, revealing a significant anthelmintic effect in the C. medica fruit peel extract compared to the standard drug. Antimicrobial properties were assessed against five test bacterial and fungal strains. Antibacterial tests showed zones of inhibition ranging from 8 to 11 mm, while no prominent zones of inhibition were observed in antifungal tests. The DPPH assay demonstrated significant antioxidant properties of Citron fruit peel extract compared to the standard ascorbic acid. The Chloroform extract of citron fruit peel exhibited significant cytotoxic properties against FaDu (human hypopharyngeal tumour) cell line. The present study indicates the potential of the chloroform extract of C. medica fruit peel to be employed as an anthelmintic, antibacterial, antioxidant and anticancer agent. Hence, it emphasises the prominence that can be given to the dietary consumption of citrus fruit peel in various forms, such as dried peel, powder etc.

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References

Bairagi GB, Kabra AO, Mandade RJ. Anthelmintic activity of Citrus medica L. leaves in Indian adult earthworm. International Journal of Pharmtech Research. 2011;3:664-67.

Sunday Enejoh O, Suleiman M, Ologunja J, FolorunshoAmbali S. In vitro anthelmintic efficacy of extracts of Citrus aurantifolia (Christm) Swingle fruit peels against Heligmosomoides bakeriova and larvae. International Journal of Current Pharmaceutical Research. 2015;7:5.

Kamal GM, Anwar F, Hussain AI, Sarri N, Ashraf MY. Yield and chemical composition of Citrus essential oils as affected by drying pretreatment of peels. International Food Research Journal. 2011;18.

Panara K, Joshi K, Nishteswar K. A review on phytochemical and pharmacological properties of Citrus medica Linn. Int J Pharm Biol Arch. 2012;3:1292-97.

Rajeswari VD. Anthelmintic activity of plants: A review. Research Journal of Phytochemistry. 2014;8:57-63. https://doi.org/10.3923/rjphyto.2014.57.63

Chhikara N, Kour R, Jaglan S, Gupta P, Gat Y, Panghal A. Citrus

medica: Nutritional, phytochemical composition and health benefits - A review. Food and Function. 2018;9:1978-92. https://doi.org/10.1039/C7FO02035J

Karimi E, Jaafar H, Ghasemzadeh A, Ebrahimi M. Fatty acid composition, antioxidant and antibacterial properties of the microwave aqueous extract of three varieties of Labisia pumila Benth.Biological Research. 2015;248:9. https://doi.org/10.1186/0717-6287-48-9

FAO. Food and Agriculture Organizations of the United Nations [Internet]; 2023. Available from: http://www.fao.org/faostat/en/#data. [Accessed: June 2, 2023].

Mabberley DJ. Citrus (Rutaceae): A review of recent advances in etymology, systematics and medical applications. Blumea.2004;49:481-98. https://doi.org/10.3767/000651904X484432

Hooker JD. The Flora of British India. Reeve & Co., London. 1875;Vol. 1. https://doi.org/10.5962/bhl.title.678

Ladaniya MS. Commercial fresh Citrus cultivars and producing countries. Citrus Fruit: Biology, Technology and Evaluation. Academic Press, San Diego. 2008;13-65. https://doi.org/10.1016/B978-012374130-1.50004-8

Ballistreri G, Fabroni S, Romeo FV, Timpanaro N, Amenta M, Rapisarda P. Anthocyanins and other polyphenols in Citrus genus: Biosynthesis, chemical profile and biological activity. In: Polyphenols in Plants. 2019;191-215. https://doi.org/10.1016/B978-0-12-813768-0.00014-1

Gabriele B, Fazio A, Dugo P, Costa R, Mondello L. Essential oil composition of Citrus medica L. Cv. Diamante (Diamante citron) determined after using different extraction methods. Journal of Separation Science. 2009;32:99-108. https://doi.org/10.1002/jssc.200800404

Enejoh OS, Shuaibu K, Suleiman MM, Ajanusi JO. Evaluation of anthelmintic efficacy of Citrus aurantifolia (Christm) fruit juice against Heligmosomoides bakeri. International of Advanced Biological Research. 2014;4:448-45.

Altemimi A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot DA. Phytochemicals: Extraction, isolation and identification of bioactive compounds from plant extracts. Plants. 2017;6:42. https://doi.org/10.3390/plants6040042

Adham AN. Phytochemical analysis and evaluation antibacterial activity of Citrus medica peel and juice growing in Kurdistan/Iraq. Journal of Applied Pharmaceutical Science. 2015;5:136-41. https://doi.org/10.7324/JAPS.2015.501023

Tomotake H, Koga T, Yamato M, Kassu A, Ota F. Antibacterial activity of Citrus fruit juices against Vibrio species. Journal of Nutritional Science and Vitaminology. 2006;52:157-60. https://doi.org/10.3177/jnsv.52.157

Sah AN, Juyal V, Melkani AB. Antimicrobial activity of six different parts of the plant Citrus medica Linn. Pharmacognosy Journal. 2011;3:80-83. https://doi.org/10.5530/pj.2011.21.15

Chutia M, Deka Bhuyan PD, Pathak MG, Sarma TC, Boruah P. Antifungal activity and chemical composition of Citrus reticulata Blanco essential oil against phytopathogens from North East India. LWT - Food Science and Technology. 2009;42:777-80. https://doi.org/10.1016/j.lwt.2008.09.015 20. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;181:1199-200. https://doi.org/10.1038/1811199a0

Adnan M, Nazim Uddin Chy M, Mostafa Kamal AT, Azad MO, Paul A, Uddin SB et al. Investigation of the biological activities and characterization of bioactive constituents of Ophiorrhiza rugosa var. prostrata (D. Don) & Mondal leaves through in vivo, in vitro and in silico approaches. Molecules. 2019;24:1367. https://doi.org/10.3390/molecules24071367

Pu ZH, Zhang YQ, Yin ZQ, Jiao XU, Jia RY, Yang LU, Fan YA. Antibacterial activity of 9-octadecanoic acid-hexadecanoic acidtetrahydrofuran-3, 4-diyl ester from neem oil. Agricultural Sciences in China. 2010;9:1236-40. https://doi.org/10.1016/S1671-

(09)60212-1

Chang RS, Ding L, Gai-Qing C, Qi-Choa P, Ze-Lin Z, Smith KM. Dehydro andrographolide succinic acid monoester as an inhibitor against the human immunodeficiency virus. Experimental Biology and Medicine. 1991;197:59-66. https://

doi.org/10.3181/00379727-197-43225

Abubakar MN, Majinda RR. GC-MS analysis and preliminary antimicrobial activity of Albizia adianthifolia (Schumach) and Pterocarpus angolensis (DC). Medicines. 2016;3:3. https://doi.org/10.3390/medicines3010003

Doshi GM, Kande PP. Evalution of bioactivity of Cucurbita pepo L., Cucumis melo L. and Cucumis sativus L. Seed extracts. Indian Journal of Experimental Biology. 2019;57:5.

Herdewijn P, De Clercq E. The cyclohexene ring as bioisostere of a furanose ring: Synthesis and anti-viral activity of cyclohexenyl nucleosides. Bioorganic and Medicinal Chemistry Letters. 2001;11:1591-97. https://doi.org/10.1016/S0960-894X(01)00270-0

Banakar P, Jayaraj M. Pharmacognosy, phytochemistry and GCMS analysis of ethanolic stem extract of Waltheria indica L. - A

potent medicinal plant. Journal of Biologically Active Products from Nature. 2017;7:369-78. https://doi.org/10.1080/22311866.2017.1400923

MJ DM, Radhamany PM, Jalaj AV. Pharmacognostic studies on leaf of Tamilnadia uliginosa (Retz.) Tirveng. & Sastre (Rubiaceae). International Journal. 2015;3:118-23.

Rajeev R, Marathe SD, Niranjan V, Sharma B, Sarojini S. In silico analysis of stigmasterol from Saraca asoca as a potential therapeutic drug against alzheimer's disease. Journal of Biologically Active Products from Nature. 2021;11:516-29. https://doi.org/10.1080/22311866.2021.1970021

Suresh T, Sarojini S. In vivo, in vitro and in silico screening of a potent Angiotensin Converting Enzyme (ACE) inhibitor from Trigonella foenum-graecum extract using Zebrafish as a model organism to reduce hypertension. Research Journal of Biotechnology. 2020;15:9-16.

Misra S, Kootery KP, Sarojini S. Inhibitory potential of Ferulaassa foetida extract on L-type calcium channel protein revealed by Zebrafish studies and molecular docking. Asian Journal of Chemistry. 2021;21:2353-59. https://doi.org/10.14233/ajchem.2021.23326

Espina L, Somolinos M, Lorán S, Conchello P, García D, Pagán R. Chemical composition of commercial Citrus fruit essential oils and evaluation of their antimicrobial activity acting alone or in combined processes. Food Control. 2011;22:896-902.https://doi.org/10.1016/j.foodcont.2010.11.021

Wang F, You H, Guo Y, Wei Y, Xia P, Yang Z, Yang D. Essential oils from three kinds of fingered citrons and their anti-bacterial activities. Industrial Crops and Products. 2020;147:112172. https://doi.org/10.1016/j.indcrop.2020.112172

Li ZH, Cai M, Liu YS, Sun PL, Luo SL. Antibacterial activity and mechanisms of essential oil from Citrus medica L. var. sarcodactylis. Molecules. 2019;24:1577. https://doi.org/10.3390/molecules24081577

Masoko P, Matotoka MM, Mphosi MS. Phytochemical analysis and antibacterial activity of Citrullus lanatus var. citroides (Citron watermelon) fruit and the effect of temperature on the biological activity of the rind. South African Journal of Botany.2022;150:1111-21. https://doi.org/10.1016/j.sajb.2022.09.024

Jadhav A. A study on in vitro antihelmintic activity of aqueous and methanolic extracts of Achyranthes aspera L.. leaves against Pheritima posthuma. World Journal of Pharmacy and Pharmaceutical Sciences. 2017;1600-06. https://doi.org/10.20959/wjpps20178-9787

Dhargyal S, Philip I, Biswas S, Sarojini S. Anti-vibrio effects of the precious Tibetan pill, Rinchen Drangjor Rilnag Chenmo (RDRC). Plant Science Today. 2021;8:681-87. https://doi.org/10.14719/pst.2021.8.3.1225

Abdelqader A, Qarallah B, Al-ramamneh D, Das G. Anthelmintic effects of Citrus peels ethanolic extracts against Ascaridia galli. Veterinary Parasitology. 2012;188:78-84. https://doi.org/10.1016/j.vetpar.2012.03.003

Mahmud S, Saleem M, Siddique S, Ahmed R, Khanum R, Perveen Z. Volatile components, antioxidant and antimicrobial activity of Citrus acida var. sour lime peel oil. Journal of Saudi Chemical Society. 2009;13:195-98. https://doi.org/10.1016/j.jscs.2009.03.001

Madhuri S, Hegde AU, Srilakshmi NS, PrashithKekuda TR. Antimicrobial activity of Citrus sinensis and Citrus aurantium peel

extracts. Journal of Pharmaceutical and Scientific Innovation (JPSI). 2014;3:366-68. https://doi.org/10.7897/2277-4572.034174

Sreekumar MB, Annadurai N, Jayaram S, Sarojini S. Industrial applications of hybrid nanocatalysts and their green synthesis. Topics in Catalysis. 2022.

Sarojini S, Dhargyal S, Philip I, Biswas S, Chavan PG, Rao NR. A new hope to green nano-biomedical science and technical utilization in green nanoarchitectonics, Jenny Stanford. 2023;225-48. https://doi.org/10.1201/9781003318606-10

Fratianni F, Cozzolino A, De Feo V, Coppola R, Ombra MN, Nazzaro F. Polyphenols, antioxidant, antibacterial and biofilm inhibitory activities of peel and pulp of Citrus medica L., Citrus bergamia and Citrus medica cv. Salò cultivated in Southern Italy.Molecules. 2019;24:4577. https://doi.org/10.3390/molecules24244577

Chen Q, Wang D, Tan C, Hu Y, Sundararajan B, Zhou Z. Profiling of flavonoid and antioxidant activity of fruit tissues from 27 Chinese local Citrus cultivars. Plants. 2020;9. https://doi.org/10.3390/plants9020196

Liu S, Li S, Ho CT. Dietary bioactives and essential oils of lemon and lime fruits. Food Science and Human Wellness. 2022;11:753-64. https://doi.org/10.1016/j.fshw.2022.03.001

Kundla JL, Sahithya P. A comprehensive review on medicinal plants used in the treatment of anticancer activity. International Journal of Advanced Research in Pharmaceutical Sciences.2021;1:28-43.

Entezari M, Majd A, Falahian FA, Mehrabian S, Hashemi M, ardeshiri LA. Antimutagenicity and anticancer effects of Citrus medica fruit juice. Acta Medica Iranica. 2009;47:373-77.

Hirata T, Fujii M, Akita K, Yanaka N, Ogawa K, Kuroyanagi M, Hongo D. Identification and physiological evaluation of the components from Citrus fruits as potential drugs for anticorpulence and anticancer. Bioorganic and Medicinal Chemistry. 2009;17:25-28. https://doi.org/10.1016/j.bmc.2008.11.039

Tunjung WA, Cinatl Jr J, Michaelis M, Smales CM. Anti-cancer effect of kaffir lime (Citrus hystrix DC) leaf extract in cervical cancer and neuroblastoma cell lines. Procedia Chemistry.2015;14:465-68. https://doi.org/10.1016/j.proche.2015.03.062

Karimi E, Oskoueian E, Hendra R, Oskoueian A, Jaafar HZ. Phenolic compounds characterization and biological activities of Citrus aurantium bloom. Molecules. 2012;17:1203-18. https://doi.org/10.3390/molecules17021203

Published

20-01-2024 — Updated on 25-01-2024

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Jayaram S, Sarojini S, Anand SB, Raj AAI, Parakadan A, Philip I, Biswas S. Citrus for wellness: Exploring the bioactive properties of Citrus medica fruit peel with emphasis on its anticancer, antioxidant, antimicrobial and anthelmintic properties. Plant Sci. Today [Internet]. 2024 Jan. 25 [cited 2024 Nov. 21];11(1):616-25. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/2612

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