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Plant growth-promoting properties of endophytic bacteria isolated from some xerophytic plants distributed in arid regions (Uzbekistan)

Authors

  • Ixtiyor Akramov Department of Genetics and Biotechnology, Samarkand State University named after Sharof Rashidov, University Boulevard, 15, Samarkand, 140100, Uzbekistan https://orcid.org/0000-0001-5041-0428
  • Shahzod Axanbayev Department of Genetics and Biotechnology, Samarkand State University named after Sharof Rashidov, University Boulevard, 15, Samarkand, 140100, Uzbekistan https://orcid.org/0000-0002-9699-8869
  • Begali Alikulov Самаркандский государственный университет
  • Sitora Mukhtorova Department of Agrobiotechnology,, Tashkent branch of Samarkand State Veterinary Medicine, Animal Husbandry and Biotechnology University, Chilonzor district 20, 35A, Tashkent, 100117, Uzbekistan https://orcid.org/0009-0000-5181-7975
  • Azamat Ergashev Department of Biology, ,Denau institute of entrepreneurship and pedagogy, Guli Rano street, 1A, Denau district, Surkhandarya region, 190507, Uzbekistan https://orcid.org/0009-0003-3807-3854
  • Zafar Ismailov Department of Genetics and Biotechnology, Samarkand State University named after Sharof Rashidov, University Boulevard, 15, Samarkand, 140100, Uzbekistan https://orcid.org/0009-0003-2056-6140

DOI:

https://doi.org/10.14719/pst.2725

Keywords:

antifungal property, arid region, Ceratoides eversmanniana (Stschegl. ex I.G.Borshch.) Botsch. & Ikonn., endophytic bacteria, Kochia prostrata (L.) Schrad., plant growth-promoting properties

Abstract

Recent advancements in the development of endophytic microorganisms-based stimulants have shown promising potential in various fields. Research on the identification of these endophytic microorganisms has been well reported, however, there are limited studies of these endophytes isolated from plants in arid regions. Thus, isolation and identification of promising microbial endophytes from xerophytic plants is essential in technology development for sustainable agriculture in arid regions. This study aims to identify the endophytic bacteria isolated from Kochia prostrata (L.) Schrad. and Ceratoides eversmanniana (Stschegl. ex I.G.Borshch.) Botsch. & Ikonn. in vertical zones of arid regions of Uzbekistan, and examine their potential plant growth-promoting properties. Using Luria-Bertani (LB) medium, 70 distinct bacterial colony were isolated from the different segments of K. prostrata and C. eversmanniana. These isotypes were screened using NaCl-supplemented LB medium in which nine promising bacterial isolates showed tolerance to 15% NaCl. The nine promising halophytes were subjected to molecular identification using specific primers. The isolates from K. prostrata are identified as Bacillus amyloliquefaciens, Bacillus pumilus, Priestia aryabhattai, Pseudomonas putida, and Priestia endophytica. On the other hand, Priestia megaterium, Pseudomonas putida, Bacillus subtilis and Brevibacillus parabrevis were isolated from C. eversmanniana. The identified isolates also showed significant plant growth-promoting properties (N2-fixation, IAA production, phosphates solubilization, ACC deaminase production, siderophores production) and shows ability to inhibit pathogenic fungal growth. Based on the result, the identified bacterial endophytes can be processed as growth-stimulants and biological control of fungal pathogens in crops in arid regions.

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Published

14-08-2023

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How to Cite

1.
Ixtiyor Akramov, Shahzod Axanbayev, Alikulov B, Sitora Mukhtorova, Azamat Ergashev, Zafar Ismailov. Plant growth-promoting properties of endophytic bacteria isolated from some xerophytic plants distributed in arid regions (Uzbekistan) . Plant Sci. Today [Internet]. 2023 Aug. 14 [cited 2024 Nov. 24];. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/2725

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