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A modified CTAB method for extracting high-quality genomic DNA from aquatic plants

Authors

DOI:

https://doi.org/10.14719/pst.2850

Keywords:

Plant DNA extraction, CTAB based DNA extraction, genomic DNA, PCR amplification, RAPD primers

Abstract

This study introduces a streamlined approach for extractinghigh-quality DNA fromaquatic plants using CTAB, catering to molecular studies. Seven aquatic plant species(Hygrophilaauriculata, Limnophilarepens, Crinum malabaricum, Lagenandraovata, Ludwigiaperuviana, Eichhorniacrassipes, and Ipomoea aquatica) spanning six orderswere subjected toDNA extraction. The method combinesmechanical lysis and chemical treatments to effectively disrupt cells, coupled with RNase treatment and phenol extraction to mitigate RNA and protein contamination. The optimizedCTAB protocol facilitatesthe extraction of high-quality genomic DNA, suitable for amplifying plant barcode genes such as ITS and rbcL, as well asmarkerslike RAPD and ISSR, thereby enhancing the efficiency and reliability of genomic studies in aquatic plants.

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References

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Published

17-02-2024

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How to Cite

1.
Valiya Thodiyil J, Edathumthazhe Kuni S, Nediyaparambu Sukumaran P. A modified CTAB method for extracting high-quality genomic DNA from aquatic plants. Plant Sci. Today [Internet]. 2024 Feb. 17 [cited 2024 Nov. 21];. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/2850

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Research Articles

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