Kigelia africana (Lam.) Benth. fruit inhibits iron-induced lipid peroxidation and ?-amylase enzyme activity
DOI:
https://doi.org/10.14719/pst.2873Keywords:
Diabetes, Malondialdehyde, ferric reducing antioxidant property, Fe2 chelating abilities, Kigelia africana fruitAbstract
Insufficiencies in insulin secretion and/or action cause diabetes mellitus (DM), a complex condition characterized by abnormal blood glucose levels. Diabetes is often treated by modifying either glucose metabolism, lipids metabolism, or both. A direct correlation has been found between high levels of lipid peroxide in individuals with diabetes and lower intracellular antioxidant activity. Therefore, this study was designed to investigate the effect of the aqueous extract of Kigelia africana fruit on the carbohydrate hydrolyzing enzyme, ?-amylase, and FeSO4-induced lipid peroxidation in the rat pancreas. The in vitro antioxidant potential, including assays for (1,1 diphenyl-2-picryl hydrazyl (DPPH), ferric reducing antioxidant property (FRAP), nitric oxide (NO), hydroxyl (OH) scavenging, and Fe2+ chelating abilities, of the fruit extract was also evaluated. The effect of the fruit extract on the ?-amylase enzyme, and FeSO4-induced lipid peroxidation in the rat pancreas was also evaluated. K. africana at a concentration of 0.5 mg/mL and 1 mg/mL scavenged DPPH radicals, with the lower concentration showing significant differences compared to the control. The K. africana fruit extract reduced ferric compounds significantly compared to the standard. The OH* radical scavenging results revealed that all concentrations of K. africana fruit scavenged OH* radicals without significant differences compared to the control. K. africana fruit also chelated iron significantly compared to the control. The fruit extract inhibited iron-induced lipid peroxidation in the rat pancreas and ?-amylase activity compared to the control. K. africana fruit displayed promising potential in inhibiting ?-amylase activity and associated lipid peroxidation in diabetics. Therefore, it holds strong promise for use in diabetes treatment, validating its traditional use for managing the condition.
Downloads
References
Zhang BB, Moller DE. New approaches in the treatment of type 2 diabetes. Curr Opin Chem Biol. 2000;4:461-67. https://doi.org/10.1016/S1367-5931(00)00103-4
Burke JP, Williams K, Narayan KMV et al. A population perspective on diabetes prevention: whom should we target for preventing weight gain. Diabetes Care. 2003;26:1999-2004. https://doi.org/10.2337/diacare.26.7.1999
Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes:
estimates for the year 2000 and projections for 2030. Diabetes Care. 2004;27:1047-53.
Vats RK, Kumar V, Kothari A et al. Emerging targets for diabetes. Curr Sci.
;88:241-49.
Gokce N, Keaney JF Jr, Hunter LM, Watkins MT, Menzoian JO, Vita JA. Risk stratification for postoperative cardiovascular events via noninvasive assessment of endothelial function: A prospective study. Circulation. 2002;105:1567-72. https://doi.org/10.1161/01.CIR.0000012543.55874.47
Morrish NJ, Stevens LK, Head J, Fuller JH, Jarrett RJ, Keen H. A prospective study of mortality among middle-aged diabetic patients (the London cohort of the WHO multinational study of vascular disease in diabetics) I: Causes and death rates.
Diabetologia. 1990;33:538-41. https://doi.org/10.1007/BF00404141
Morrish NJ, Wang SL, Stevens LK, Fuller JH, Keen H. Mortality and causes of death in the WHO multinational study of vascular disease in diabetes. Diabetologia. 2001;44:S14-S21. https://doi.org/10.1007/PL00002934
de Jager J, Dekker JM, Kooy A, Kostense PJ, Nijpels G, Heine RJ. et al. Endothelial dysfunction and low-grade inflammation explain much of the excess cardiovascular mortality in individuals with type 2 diabetes: The Hoorn Study. Arter Thromb Vasc Biol. 2006;26:1086-93. https://doi.org/10.1161/01.ATV.0000215951.36219.a4
Gianazza E, Brioschi M, Fernandez AM, Banfi C. Lipoxidation in cardiovascular diseases. Redox Biol. 2019;23:101119-40. https://doi.org/10.1016/j.redox.2019.101119
Ramana KV, Srivastava S, Singhal SS. Lipid peroxidation products in human health and disease. Oxidative Med Cell Longev. 2019;7147235-37. https://doi.org/10.1155/2019/7147235
Njie-Mbye YF, Chitnis M, Opere C, Ohia S. Lipid peroxidation: Pathophysiological and pharmacological implications in the eye. Front Physiol. 2003;4:366-75. https://doi.org/10.3389/fphys.2013.00366
Fatani SH, Babakr AT, NourEldin EM, Almarzouki AA. Lipid peroxidation is associated with poor control of type-2 diabetes mellitus, diabetes and metabolic syndrome. Clin Res Rev. 2016;10:S64-S67. https://doi.org/10.1016/j.dsx.2016.01.028
Davì GA, Falco A, Patrono C. Lipid peroxidation in diabetes mellitus. Antioxid Redox Signal. 2005;7:256-68. https://doi.org/10.1089/ars.2005.7.256
Tangvarasittichai O, Tangvarasittichai S. Oxidative stress, ocular disease and diabetes retinopathy. Curr Pharm Des. 2018;24:4726-41. https://doi.org/10.2174/1381612825666190115121531
Kesavulu MM, Rao BK, Giri R, Vijaya J, Subramanyam G, Apparao C. Lipid peroxidation and antioxidant enzyme status in Type 2 diabetics with coronary heart disease. Diabetes Res Clin Pract. 2001;53:33-39. https://doi.org/10.1016/S0168-8227(01)00238-8
Abdollahi M, Farshchi A, Nikfar S, Seyedifar M. Effect of chromium on glucose and lipid profiles in patients with type 2 diabetes; a meta-analysis review of randomized trials. J Pharm Pharm Sci. 2013;16:99-114. https://doi.org/10.18433/J3G022
Rafieian-Kopaei M, Nasri H. Ginger and diabetic nephropathy. J Renal Inj Prev. 2013;2:9-10.
Nasri H, Rafieian-Kopaei M. Tubular kidney protection by antioxidants. Iran J Public Health. 2013;42:1194-96.
Pushparaj P, Tan CH, Tan BKH. Effects of Averrhoa bilimbi leaf extract on blood glucose and lipids in streptozocin-diabetic rats. J Ethnopharmacol. 2000;72:69-76. https://doi.org/10.1016/S0378-8741(00)00200-2
Tahrani AA, Piya MK, Kennedy A, Barnett AH. Glycaemic control in type 2 diabetes: targets and new therapies. Pharmacol Ther. 2010;125:328-61. https://doi.org/10.1016/j.pharmthera.2009.11.001
Lam KS. New aspects of natural products in drug discovery. Trends Microbiol. 2007;15:279-89. https://doi.org/10.1016/j.tim.2007.04.001
Dragan S, Andrica Maria F, Maria-Corina S, Timar R. Polyphenols-rich natural products for treatment of diabetes. Curr Med Chem. 2014;21:38. https://doi.org/10.2174/0929867321666140826115422
Bello I, Shehu MW, Musa M, Asmawi MZ, Mahmud R. Kigelia africana (Lam.) Benth.(Sausage tree): Phytochemistry and pharmacological review of a quintessential African traditional medicinal plant. Journal of Ethnopharmacology. 2016 Aug 2;189:253-76. https://doi.org/10.1016/j.jep.2016.05.049
Guidigan ML, Azihou F, Idohou R, Okhimamhe AA, Fandohan AB, Sinsin B et al. Modelling the current and future distribution of Kigelia africana under climate change in Benin, West Africa. Modeling Earth Systems and Environment. 2018 Sep;4:1225-38. https://doi.org/10.1007/s40808-018-0491-4
Meminvegni Landry GG, Fortuné A, Appollonia Aimiosino O, Brice S, Brahim SU, Lucette A. Examining the effectiveness of a protected areas network in the conservation of Kigelia africana under climate change by 2050 in Benin.
Nabatanzi A, M Nkadimeng S, Lall N, Kabasa JD, J McGaw L. Ethnobotany, phytochemistry and pharmacological activity of Kigelia africana (Lam.) Benth.(Bignoniaceae). Plants. 2020 Jun 15;9(6):753. https://doi.org/10.3390/plants9060753
Ogunlakin AD, Sonibare MA, Yeye OE, Gyebi GA, Ayokunle DI, Arigbede OE et al. Isolation and characterization of novel hydroxyflavone from Kigelia africana (Lam.) Benth. fruit ethyl acetate fraction against CHO 1 and HeLa cancer cell lines: In vitro and in silico studies. Journal of Molecular Structure. 2023 Jun 15;1282:135180. https://doi.org/10.1016/j.molstruc.2023.135180
Oyaizu M. Studies on products of browning reactions: Antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr. 1986;44:629-32. https://doi.org/10.5264/eiyogakuzashi.44.307
Gyamfi MA, Yonamine M, Aniya Y. Free-radical scavenging action of medicinal herbs from Ghana: Thonningia sanguinea on experimentally-induced liver injuries. General Pharmacology: The Vascular System. 1999 Jun 1;32(6):661-67. https://doi.org/10.1016/S0306-3623(98)00238-9
Minotti G, Aust SD. The requirement for iron (III) in the initiation of lipid peroxidation by iron (II) and hydrogen peroxide. Journal of Biological Chemistry. 1987 Jan 25;262(3):1098-104. https://doi.org/10.1016/S0021-9258(19)75755-X
Oboh G, Puntel RL, Rocha JB. Hot pepper (Capsicum annuum, Tepin and Capsicum chinese, Habanero) prevents Fe2+-induced lipid peroxidation in brain–In vitro. Food Chemistry. 2007 Jan 1;102(1):178-85. https://doi.org/10.1016/j.foodchem.2006.05.048
Halliwell B, Gutteridge JM. Formation of a thiobarbituric-acid-reactive substance from deoxyribose in the presence of iron salts: The role of superoxide and hydroxyl radicals. FEBS Letters. 1981 Jun 15;128(2):347-52. https://doi.org/10.1016/0014-5793(81)80114-7
Devi M, Kumar P, Singh R, Sindhu J, Kataria R. Design, synthesis, spectroscopic characterization, single crystal X-ray analysis, In vitro ?-amylase inhibition assay, DPPH free radical evaluation and computational studies of naphtho [2, 3-d] imidazole-4, 9-dione appended 1, 2, 3-triazoles. European Journal of Medicinal Chemistry. 2023 Mar 15;250:115230. https://doi.org/10.1016/j.ejmech.2023.115230
Bellé NA, Dalmolin GD, Fonini G, Rubin MA, Rocha JB. Polyamines reduces lipid peroxidation induced by different pro-oxidant agents. Brain Research. 2004 May 22;1008(2):245-51. https://doi.org/10.1016/j.brainres.2004.02.036
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry. 1979 Jun 1;95(2):351-58. https://doi.org/10.1016/0003-2697(79)90738-3
Aziz MA, Diab AS, Mohammed AA. Antioxidant categories and mode of action. Antioxidants. 2019 Nov 6;2019:3-22.
Hussain T, Fatima I, Rafay M, Shabir S, Akram M, Bano S. Evaluation of antibacterial and antioxidant activity of leaves, fruit and bark of Kigelia africana. Pak J Bot. 2016;48(1):277-83.
Zhang C, Feng S, Wang Q, Wang P, Xu J, Chen T. Flavonoids and phenolic compounds from Smilax scobinicaulis. Chem Nat Compd. 2014;50(2):254-57. https://doi.org/10.1007/s10600-014-0925-9
Admassu S, Kebede M. Application of antioxidants in food processing industry: Options to improve the extraction yields and market value of natural products. Adv Food Technol Nutr Sci. 2019;5:38-49. https://doi.org/10.17140/AFTNSOJ-5-155
Gulcin ?. Antioxidants and antioxidant methods: An updated overview. Archives of toxicology. 2020 Mar;94(3):651-715. https://doi.org/10.1007/s00204-020-02689-3
Nieto G. How are medicinal plants useful when added to foods?. Medicines. 2020 Sep 14;7(9):58. https://doi.org/10.3390/medicines7090058
Sadiq IZ. Free radicals and oxidative stress: Signaling mechanisms, redox basis for human diseases and cell cycle regulation. Current Molecular Medicine. 2023 Jan 1;23(1):13-35. https://doi.org/10.2174/1566524022666211222161637
Stoia M, Oancea S. Low-molecular-weight synthetic antioxidants: Classification, pharmacological profile, effectiveness and trends. Antioxidants. 2022 Mar 26;11(4):638. https://doi.org/10.3390/antiox11040638
Girish YR, Sharath Kumar KS, Prashantha K, Rangappa S, Sudhanva MS. Significance of antioxidants and methods to evaluate their potency. Materials Chemistry Horizons. 2023 May 1;2(2):93-112.
Islam M, Prottay AA, Sultana I, Al Faruq A, Bappi MH, Akbor MS et al. Phytochemical screening and evaluation of antioxidant, anti-inflammatory, antimicrobial and membrane-stabilizing activities of different fractional extracts of Grewia nervosa (Lour.) Panigrahi. Food Bioscience. 2023 Aug 1;54:102933. https://doi.org/10.1016/j.fbio.2023.102933
Anusha Siddiqui S, Redha AA, Esmaeili Y, Mehdizadeh M. Novel insights on extraction and encapsulation techniques of elderberry bioactive compounds. Critical Reviews in Food Science and Nutrition. 2023 Aug 29;63(22):5937-52. https://doi.org/10.1080/10408398.2022.2026290
Jyotirmayee B, Mahalik G. A review on selected pharmacological activities of Curcuma longa L. International Journal of Food Properties. 2022 Dec 31;25(1):1377-98. https://doi.org/10.1080/10942912.2022.2082464
Akintunde JK, Akintunde DG, Irondi EA, Babaita K, Labaika R, Sunday O. Antioxidants from stem bark of Kigelia africana inhibits free radicals and membrane lipid damage in rat testes in vitro. Oxidants and Antioxidants in Medical Science. 2016;5(2):63. https://doi.org/10.5455/oams.240516.or.097
Alkhatib A. Antiviral functional foods and exercise lifestyle prevention of coronavirus. Nutrients. 2020 Aug 28;12(9):2633. https://doi.org/10.3390/nu12092633
Ogunlakin AD, Sonibare MA, Jabeen A, Shah SF, Shaheen F. Antioxidant and anti-proliferative studies on Kigelia africana (Lam.) Benth. and its constituents. Tropical Journal of Natural Product Research (TJNPR). 2021 Mar 1;5(3):570-75. doi. org/10.26538/tjnpr/v5i3. 25.
Fagbohun OF, Olawoye B, Ademakinwa AN, Jolayemi KA, Msagati TA. Metabolome modulatory effects of Kigelia africana (Lam.) Benth. fruit extracts on oxidative stress, hyperlipidaemic biomarkers in STZ-induced diabetic rats and antidiabetic effects in 3T3 L1 adipocytes. Journal of Pharmacy and Pharmacology. 2020 Dec;72(12):1798-811. https://doi.org/10.1111/jphp.13362
Ogunlakin AD, Ojo OA, Gyebi GA, Akinwumi IA, Adebodun GO, Ayokunle DI et al. Elemental evaluation, nutritional analysis, GC-MS analysis and ameliorative effects of Artocarpus communis JR Forst. & G. Forst. seeds’ phytoconstituents on metabolic syndrome via in silico approach. Journal of Biomolecular Structure and Dynamics. 2023 Dec;11:1-21. https://doi.org/10.1080/07391102.2023.2293271
Ojo OA, Rotimi DE, Ojo AB, Ogunlakin AD, Ajiboye BO. Gallic acid abates cadmium chloride toxicity via alteration of neurotransmitters and modulation of inflammatory markers in Wistar rats. Scientific Reports. 2023 Jan 28;13(1):1577. https://doi.org/10.1038/s41598-023-28893-6
Ojo OA, Ogunlakin AD, Gyebi GA, Ayokunle DI, Odugbemi AI, Babatunde DE et al. Profiling the antidiabetic potential of GC–MS compounds identified from the methanolic extract of Spilanthes filicaulis: experimental and computational insight. Journal of Biomolecular Structure and Dynamics. 2023 Dec 6;1-22. https://doi.org/10.1080/07391102.2023.2291828
Fagbohun OF, Oriyomi OV, Adekola MB, Msagati TA. Biochemical applications of Kigelia africana (Lam.) Benth. fruit extracts in Diabetes mellitus. Comparative Clinical Pathology. 2020 Dec;29:1251-64. https://doi.org/10.1007/s00580-020-03179-9
Singh A, Kumari S, Singh AK, Singh NK. Ethnopharmacology and pharmacology of Kigelia africana (Lam.) Benth. Int J Green Pharm. 2018;11:S23-31.
Maritim A, Dene BA, Sanders RA, Watkins III JB. Effects of ??carotene on oxidative stress in normal and diabetic rats. Journal of Biochemical and Molecular Toxicology. 2002;16(4):203-08. https://doi.org/10.1002/jbt.10038
Ojo OA, Ogunlakin AD, Maimako RF, Gyebi GA, Olowosoke CB, Taiwo OA et al. Therapeutic study of cinnamic acid derivative for oxidative stress ablation: The computational and experimental answers. Molecules. 2023 Nov 4;28(21):7425. https://doi.org/10.3390/molecules28217425
Ezuruike UF, Prieto JM. The use of plants in the traditional management of diabetes in Nigeria: Pharmacological and toxicological considerations. Journal of Ethnopharmacology. 2014;155(2):857-924. https://doi.org/10.1016/j.jep.2014.05.055
Ogunlakin AD, Onifade TR, Ojo OA, Adesanya EO, Berena GA, Ayeni PO et al. Antidiabetic potential of Carica papaya L. and its constituents: From folkloric uses to products development. Bioactive Compounds in Health and Disease. 2023 Jun 30;6(6):126-44. https://doi.org/10.31989/bchd.v6i6.1108
Ojo OA, Ogunlakin AD, Gyebi GA, Ayokunle DI, Odugbemi AI, Babatunde DE et al. GC-MS chemical profiling, antioxidant, anti-diabetic and anti-inflammatory activities of ethyl acetate fraction of Spilanthes filicaulis (Schumach. And Thonn.) CD Adams leaves: experimental and computational studies. Frontiers in Pharmacology. 2023;14. https://doi.org/10.3389/fphar.2023.1235810
Eichler HG, Korn A, Gasic S, Prison W, Businger J. The effect of new specific á amylase inhibitor on post-prandial glucose and insulin excursions in normal subjects and Type 2 (non insulin dependent) diabetic patients. Diabetologia. 1984;26(4):278-81. https://doi.org/10.1007/BF00283650
Tarling CA, Woods K, Zhang R, Brastianos HC, Brayer GD, Andersen RJ et al. The search for novel human pancreatic á-amylase inhibitors: High-throughput screening of terrestrial and marine natural product extracts. ChemBioChem. 2008;9:433-38. https://doi.org/10.1002/cbic.200700470
Sonibare MA, Ogunlakin AD. Antidiabetic and antioxidant effects of methanol extract and fractions of Glyphaea brevis (Spreng.) Monachino in alloxan-induced diabetic rats. West African Journal of Pharmacy. 2016;27(1):42-53.
Kim YM, Jeong YK, Wang MH, Lee WY, Rhee HI. Inhibitory effect of Pine on hyperglycemia. Nutrition. 2005;21:756-61. https://doi.org/10.1016/j.nut.2004.10.014
Downloads
Published
Versions
- 16-05-2024 (2)
- 13-04-2024 (1)
How to Cite
Issue
Section
License
Copyright (c) 2022 Esther Oremeyi Faboro, Akingbolabo Daniel Ogunlakin, Peluola Olujide Ayeni, Oluwatunmise Deborah Opaleye, Oluwafemi Adeleke Ojo, Omolola Adenike Ajayi-Odoko, Damilare IyinKristi Ayokunle, Mubo Adeola Sonibare, Abdullah Rajaa Alanzi
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
