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Plant Science Today (PST)

Research Articles

Vol. 11 No. 3 (2024)

In vitro acute gamma radiation on tissue of pink and white lotus (Nelumbo nucifera Gaertn.) in Thailand

DOI
https://doi.org/10.14719/pst.3005
Submitted
10 October 2023
Published
15-07-2024 — Updated on 17-07-2024
Versions

Abstract

This research aimed to investigatethe age of embryos of pink and white lotus for in vitropropagation and examine the sensitivity of young lotus plants derived from tissue culture to acute gamma radiation. The seeds of both lotus varieties were 4-week, 6-week and 8-week-old embryos cultivated on solid MS medium. They were then transferred to solid MS medium supplemented with 3 mg/L BA. After culturing, the 6-week-old pink lotus embryos had an average maximum petiole length of 16.78 ± 1.01 cm and an average of 11.84 ± 0.44 roots/explant, while the 6-week-old white lotus embryos had an average maximum petiole length of 15.48 ± 0.68 cm and an average of 11.76 ± 0.70 roots/explant. Each young lotus plant produced one shoot. After treating the young lotus plants with liquid MS medium supplemented with 3 mg/L BA, both lotus varieties showed increased shoot numbers at 3.54 ± 0.13 and 3.40 ± 0.91 shoots/explant. Young lotus plants of both varieties were exposed to gamma radiation levels of 0, 20, 30 and 40 Gy. The LD50 value for pink lotus was 26.138 Gy, while the LD50 value for the white lotus was 32.031 Gy.The LD50 value will offer vital information for determining the optimal dosage of gamma radiation for both pink and white lotus in vitro.

References

  1. Lin Z, Zhang C, Cao D, Damaris RN, Yang P. The latest studies on lotus (Nelumbo nucifera). An emerging horticultural model plant. Int J Mol Sci. 2019;20(15):3680. https://doi:10.3390/ijms20153680
  2. Hongpakdee P. Sacred lotus: Potential of thai flower through ASEAN market.Khon Kaen Agr J. 2013;41(3):213-20.
  3. Thai crude drugs of the faculty of pharmacy.[Internet]. Ubon Ratchathani University; 2023. [cited 20 Aug 2023]. Available from: https://apps.phar.ubu.ac.th/thaicrudedrug/main. php?action=viewpage &pid=24
  4. Yu X, Sheng J, Zhao L, Diao Y, Zheng X, Xie K, Hu Z.In vitro plant regeneration of lotus (Nelumbo nucifera). Open Life Sci. 2015;10(1):142-46. https://doi.org/10.1515/biol-2015-0016
  5. Liu Q, Zhang D, Liu F, Qin M, Tian D. Micropropagation of Nelumbo nucifera ‘Weishan Hong’through germfree mature embryos. In Vitro Cell DevBiol. 2019;55:305-12. https://doi.org/10.1007/s11627-019-09984-4
  6. Puripunyavanich V, Boonsirichai K. Induced mutation in yellow lotus by gamma irradiation. In: Proceedings of 44th Kasetsart University Annual Conference: Plants. 2006 30 Jan - 2 Feb; Thailand. Bangkok; Kasetsart University Library. 2006; p. 439-44.
  7. Barampuram S, Allen G,Krasnyanski S.Effect of various sterilization procedures on the in vitro germination of cotton seeds.PCTOC. 2014;118:179-85.
  8. https://doi.org/10.1007/s11240-014-0472-x
  9. Yampul A, Dechkla M, Boonman N, Teerakathiti T, Phakpaknam P. Embryo of pink lotus and white lotus (Nelumbo nucifera Gaertn.) tissue culture and determination of seedling sensitivity to gamma rays. J Sci Technol MSU. 2020;39(5):506-13.
  10. Deng X, Xiong Y, Li J, Yang D, Liu J, Sun Het al. The establishment of an efficient callus induction system for lotus (Nelumbo nucifera). Plants. 2020;9(11):1436. https://doi.org/ 10.3390/plants9111436
  11. Noimai Y. Effects of plant growth regulators on seed germination treatment of Nymphaea gigantea ‘Atrans’ hybrid in vitroconditions. RMUTT Research Journal. 2018;17(2):54-64. https://doi.org/10.14456/rj-rmutt.2018.5
  12. Wongpiyasatid A. Mutation: For plant breeding. Edition 1st. Bangkok: Kasetsart University Press. 2017;p. 297.
  13. Padungsil N, Taechasinpitak T, Wongchowchan S, Chanchula C. Induction in Catharanthus roseus L. by acute gamma irradiation. tjst Journal. 2015;4(1):96-103.
  14. https://doi.org/ 10.14456/tjst.2015.4
  15. Soontornyatara S, Singhavorachai P, Puripunyavanich V, Taywiya P. Effect of gamma ray on morphological characteristics of Nelumbo nucifera (Roseum Plenum lotus). ActaHortic. 2017;1167:217-20. https://doi.org/10.17660/ActaHortic.2017.1167.33
  16. Limsanguan P, Chanprame S, Ungvichian I, Srinamnguen P, Chanprame S. Gamma ray induced mutation in Chrysanthemum and detection of DNA polymorphism by AFLP. Agricultural Sci J. 2017;48(3):334-45.
  17. Sherpa R, Devadas R, Bolbhat SN, Nikam TD, Penna S. Gamma radiation induced in-vitromutagenesis and isolation of mutants for early flowering and phytomorphological variations in Dendrobium 'Emma White'. Plants (Basel). 2022;11(22):3168.
  18. https://doi: 10.3390/plants11223168
  19. Pérez-Jiménez M, Tallón CI, Pérez-Tornero O. Inducing mutations in Citrus spp.: Sensitivity of different sources of plant material to gamma radiation. Appl Radiat Isot. 2020;157:109030. https://doi.org/10.1016/j.apradiso.2019.109030
  20. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962;15:473-97.https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  21. Firoz MA, Uddin ME, Amin R, Razzak MA, Manik MA, Khatun MM. Studies on the effect of various sterilization procedures for in vitro seed germination and successful micropropagation of Cucumis sativus. Int JPure Appl Biosci. 2016;4(1):75-81.
  22. https://doi.org/10.18782/2320-7051.2226
  23. Palee J. Natural propagation and micropropagation of Tupistra albiflora K. Larsen Sciand Tech RMUTT J. 2016;6(2):1-16.
  24. Soliman AS, Abbas MS. Effects of sulfuric acid and hot water pre-treatments on seed germination and seedlings growth of Cassia fistula L. AEJAES. 2013;13(1):7-15. https://doi.org/10.5829/idosi.aejaes.2013. 13.01.1914
  25. Shen-Miller J, Aung LH, Turek J, Schopf JW, Tholandi M, Yang M, Czaja A. Centuries-old viable fruit of sacred lotus Nelumbo nucifera Gaertn var. China antique. TropPlant Biol. 2013;6:53-68.https://doi.org/10.1007/s12042-013-9125-1
  26. Trang NTQ, Hong HTK, Huong VTM, Long DT. In vitro propagation of red lotus (Nelumbo nucifera Gaertn). An aquatic edible plant in Vietnam. Agric SciDig. 2021;41(spl):129136. https://doi.org/10.18805/ag.D-257
  27. ShouSY, Miao LX, Zai WS, Huang XZ, Guo DP. Factors influencing shoot multiplication of lotus (Nelumbo nucifera). Biol Plant. 2018;52:529-32.
  28. https://doi.org/10.1007/s10535-008-0103-7
  29. Beyaz R, Kahramanogullari CT, Yildiz C, Darcin ES, Yildiz M. The effect of gamma radiation on seed germination and seedling growth of Lathyrus chrysanthus Boiss. under in vitro conditions. J Environ Radio Act. 2016;162:129-33. https://doi.org/10.1016/j.jenvrad. 2016.05.006
  30. Ferdous MH, Billah AM, Mehraj H, Taufique T, Uddin AFMJ. BAP and IBA pulsing for in vitro multiplication of banana cultivars through shoot-tip culture. J Biosci Agric Res. 2015;3(2):87-95.https://doi.org/10.18801/jbar.030215.35
  31. KhatoonS, Liu W, Ding CB, Liu X, Zheng Y, Zhang Yet al. In vitroevaluation of the effects of BAP concentration and pre-cooling treatments on morphological, physiological and biochemical traits of different olive (Olea euorpea L.) cultivars. Hortic. 2022;8(12):1108. https://doi.org/10.3390/horticulturae8121108
  32. Buddharaksa P, Kaewdungtip C, U-Kong W. In vitro culture of Hippeastrum johnsonnii Bury and Caladium bicolor Vent. NUJST. 2011;19(1):18-23.
  33. Ling WT, Liew FC, Lim WY, SubramaniamS, Chew BL. Shoot induction from axillary shoot tip explants of fig (Ficus carica) cv. Japanese BTM 6. Trop Life SciRes. 2018;29(2):165-74. https://doi.org/10.21315/tlsr2018. 29.2.11
  34. Phakpaknam S, Yunchum N, Dechkla M, Pikunthong V.In vitro micropropagation and microrhizome induction of Curcuma comosaRoxb. RMUTP Research Journal. 2023;16(2):13-23. https://doi.org/10.14456/jrmutp.2022.18
  35. Pikulthong V, Phakpaknam S, Dechkla M, Teerakathiti T.Optimal medium for watercress (Alternanthera sp.) micropropagation. SSSTJ. 2018;5(2):27-32.
  36. Arunyanartl S, Soontronyatara S. Mutation induction by ? and X-ray irradiation in tissue cultured lotus. PCTOC. 2002;70:119-22.https://doi.org/10.1023/A:1016021627832
  37. Kato A, Vega JM, Han F, Lamb JC, Birchler JA. Advances in plant chromosome identification and cytogenetic techniques. Curr Opin Plant Biol. 2005;8(2):148-54.https://doi.org/10.1016/j.pbi.2005.01.014
  38. Takahashi N, Ogita N, Takahashi T, Taniguchi S, Tanaka M, Seki M, Umeda M. A regulatory module controlling stress-induced cell cycle arrest in Arabidopsis. Elife. 2019;8:e43944. https://doi.org/10.7554/eLife.43944
  39. Hoda E. In vitroinduction of flower mutation in Catharanthus roseus using gamma irradiation. Alex Sci Exch. 2014;35:64-68.https://doi.org/10.21608/ASEJAIQJSAE.2014 .2575
  40. Beyaz R. Impact of gamma irradiation pretreatment on the growth of common vetch (Vicia sativa L.) seedlings grown under salt and drought stress. Int J Radiat Biol. 2020;96(2):257-66.https://doi.org/10.1080/09553002.2020.1688885
  41. Lee JW, Jo IH, Kim JU, Hong CE, Bang KH, Park YD. Determination of mutagenic sensitivity to gamma rays in ginseng (Panax ginseng) dehiscent seeds, roots and somatic embryos. Hortic Environ Biotechnol. 2019;60:721-31. https://doi.org/10.1007/s13580-019-00164-2
  42. Pangjai W, Huehne SH. Gamma-ray induced morphological changes in rain lily (Zephyranthes spp.). Khon Kaen Agr J. 2021;49(3):643-55.
  43. https://doi.org/10.14456/kaj. 2021.57.20
  44. Hiswan J, Larekeng SH, Gusmiaty W, Putri SD. In vitro shoot induction of batik bamboo using BAP and NAA.Plant Arch. 2020;20(2):2343-49.
  45. Piyachoteskulchai N. Effect of plant growth regulator on in vitro culture of Nelumbo nucifera Gaertn. Botany [thesis]. Bangkok (Thailand): Kasetsart University; 1996.

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