Anti- psoriatic effect and phytochemical evaluation of Iraqi Scabiosa palaestina ethyl acetate extract
DOI:
https://doi.org/10.14719/pst.3055Keywords:
RP-HPLC, flavonoid compounds, antipsoriatic effectAbstract
The purpose of this study was to confirm the antipsoriasis activity of Scabiosa palaestina (S. palaestina) ariel component extract and identify the active compound(s) responsible for this activity using a developed RP-HPLC technology. The active ethyl acetate extract of S. palaestina was extracted and fractionated by the hot continuous Soxhlet apparatus method. Psoriasis was induced in experimental animals after 4 days of imiquimod (IMQ) cream application on dorsal skin rats of 3 treated groups (6 rats for each) and the normal group (6 rats) left without treatment. Two of the psoriatic-induced groups were treated with ethyl acetate (EA) extract or methotrexate (MTX) for 12 days, starting from day 4 of the IMQ application. Assessment of psoriatic lesions was done during the experiment, depending on the psoriasis area severity index (PASI). At the end of the study (day 15), animals were sacrificed and 2 skin specimens were collected for histopathological examination and cytokine measurement. Compared with the positive control group, ethyl acetate extract shows significant improvement in PASI (p-value < 0.05) and histopathological changes exerted by IMQ. On the other hand, no significant reduction has been seen in the psoriatic and angiogenic mediator (IL-17 and VEGF) with a P-value > 0.05 compared with the positive control group, which may be attributed to other cytokines that could be affected by the ethyl acetate extract of S. palaestina. These results suggest that the antipsoriasis properties of ethyl acetate extract may possibly be due to the presence of flavonoid compounds (luteolin, apigenin, quercetin, vitexin and kaempferol).
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Yamanaka K, Yamamoto O, Honda T. Pathophysiology of psoriasis: A review. J Dermatol. 2021;48(6):722-31. https:// doi: 10.1111/1346-8138.15913.
Ten Bergen LL, Petrovic A, Krogh AA, Appel S. The TNF/IL-23/IL-17 axis-head-to-head trials comparing different biologics in psoriasis treatment. Scand J Immunol. 2020;92(4):e12946. https:// doi: 10.1111/sji.12946. PMID: 32697374.
Huang YH, Chang LC, Chang YC, Chung WH, Yang SF, Su SC. Compositional alteration of gut microbiota in psoriasis treated with IL-23 and IL-17 inhibitors. Intern J Mol Sci. 2023;24(5):4568. https://doi.org/10.3390/ijms24054568 .
Kobayashi K, Chikazawa S, Chen Y, Suzuki S, Ichimasu N, Katagiri K. Oestrogen inhibits psoriasis-like dermatitis induced by imiquimod in mice in relation to increased IL-10 producing cells despite elevated expression of IL-22, IL-23, IL-17 mRNA. Exp Dermatol. 2023;32(2):203-09. https://doi.org/10.1111/exd.14688.
Huang TH, Lin CF, Alalaiwe A, Yang SC, Fang JY. Apoptotic or antiproliferative activity of natural products against keratinocytes for the treatment of psoriasis. Int J Mol Sci. 2019;20(10):2558. https://doi.org/10.3390/ijms20102558.
Gaikwad RG, Shinde AJ, Hajare AA. Herbal treatment for management of psoriasis: an overview. Research J Pharm and Tech. 2022;15(3):1385-92. https://doi.org/10.52711/0974-360X.2022.00231.
George EB, Ronald JT. Toxic plants of North America. John Wiley and Sons: Oxford, UK. 2013;p. 319-22.
Mostafa EN, Sedigheh NS. Palynological study of some Iranian species of Scabiosa L. (Caprifoliaceae). Bangladesh J Plant Taxon. 2016;23(2):215-22. https://doi.org/10.52711/0974-360X.2022.00231.
Erarslan ZB, Yesil Y. The anatomical properties of Scabiosa atropurpurea L. (Caprifoliaceae). Istanbul J Pharm. 2019;48(1):1-5. https://doi.org/10.5152/IstanbulJPharm.2018.376278 .
Carlson SE, Linder P, Donoghue MJ, Ladiges, P. The historical biogeography of Scabiosa (Dipsacaceae): Implications for old world plant disjunctions. J Biogeog. 2012;39(6):1086-100. https://doi.org/ 10.2307/23258796 .
Maroyi A. Scabiosa columbaria: A review of its medicinal uses, phytochemistry and biological activities. Asian J Pharmaceutical and Clinical Research. 2019;12(8):10-14. https://doi.org/10.22159/ajpcr.2019.v12i18.34229.
Pinto DCGA, Rahmouni N, Beghidja N, Silva AMS. Scabiosa genus: A rich source of bioactive metabolites. Medicines (Basel). 2018;5(4):110. https://doi.org/10.3390/medicines5040110
K?l?nç H. Isolation and characterization of secondary metabolites from apolar fraction of Scabiosa sicula and evaluation of their antioxidant activities. Gümü?hane Üniversitesi Fen Bilimleri Dergisi. 2021;11(3):934-42. https://doi.org/10.17714/gumusfenbil.888739.
Kasterova E, Zibareva L, Revushkin A. Secondary metabolites of some Siberian species of plants tribe Cynareae (Asteraceae). South African J Bot. 2019;125:24-29. https://doi.org/10.1016/j.sajb.2019.06.022.
Wadi K, Ibrahim K, Hammoudi H. Antibacterial effect of Scabiosa palaestina L. extract on the multidrug resistant isolates of two gram negative species. AIP Conf Proc of 1st Intern & 4th Local Conf Pure Sci (ICPS-2021); 2021 May 26-27; Diyala, Iraq. 2023.2475(1):020013. Doi//10.1063/5.0111462.
Khamees AH, JawadE, SahibHB. Investigation of the possible anti-angiogenic activity of Iraqi Scabiosa palaestina L. using ex vivo rat aorta ring assay. J Complement Med Res. 2021;12(4):249-55. https://doi.org/ 10.5455/ jcmr.2021.12.04.37.
Rahmouni N, Pinto DCGA, Beghidja N, Benayache S, Silva AMS. Scabiosa stellata L. phenolic content clarifies its antioxidant activity. Molecules. 2018;23(6):1285. https://doi.org/10.3390/molecules23061285 .
Khamees AH, Kadhim EJ. Isolation, characterization and quantification of a pentacyclic triterpinoid compound ursolic acid in Scabiosa palaestina L. distributed in the north of Iraq. Plant Science Today. 2022;9(1):178-82. https://doi.org/10.14719/pst.1398.
Shah SM, Abdul-Jalil TZ. Qualitative and quantitative estimation or chemical constituents from leaves and roots of Iraqi Agave attenuata by GC-MS and RPHPLC. Proc 10th Sci Conf, sponsored by College of Pharmacy, University of Baghdad; 2-3 June 2022. Iraqi J Pharm Sci. 2022;31(Sppl.):75-85. https://doi.org/10.31351/vol31issSuppl.pp75-85
Mattila P, Astola J, Kumpulainen J. Determination of flavonoids in plant material by HPLC with diode-array and electro-array detections. J Agri Food Chem. 2001;48(12):5834-41. https://doi.org/10.1021/jf000661f.
Parmar KM, Jagtap CS, Katare NT, Dhobi M, Prasad SK. Development of a psoriatic-like skin inflammation rat model using imiquimod as an inducing agent. Indian J Pharmacol. 2021;53(2):125-31. https://doi.org/10.4103/ijp.IJP_506_19.
Jaafar NS, Alshammaa DA, Abdul-Jalil TZ, Ibrahim NM. Quantitative determination and cytotoxic effect of oleanolic acid from Olea europaea leaves extract cultivated in Iraq. Iraqi J Pharm Sci. 2022;31(2):244-50. https://doi.org/10.31351/vol31iss2pp244-250
Kim WB, Jerome D, Yeung J. Diagnosis and management of psoriasis. Can Fam Physician. 2017;63(4):278-85. https://api.semanticscholar.org/CorpusID:43704763}
Dias DA, Urban S, Roessner U. A historical overview of natural products in drug discovery. Metabolites. 2012;2(2):303-36. https://doi.org/10.3390/metabo2020303.
Chen J, Zhong H, Huang Z, Chen X, You J, Zou T. A critical review of kaempferol in intestinal health and diseases. Antioxidants. 2023;12(8):1642. https://doi.org/10.3390/antiox12081642
Dogra S, Mahajan R. Psoriasis: Epidemiology, clinical features, co-morbidities and clinical scoring. Indian Derm Online J. 2016;7(6):471-80. https://doi.org/10.4103/2229-5178.193906
Rendon A, Schäkel K. Psoriasis pathogenesis and treatment. Int J Mol Sci. 2019;20(6):1475. https://doi.org/10.3390/ijms20061475
Hansel A, Gunther C, Ingwersen J, Starke J, Schmitz M, Bachmann M, et al. Human slan (6-sulfo LacNAc) dendritic cells are inflammatory dermal dendritic cells in psoriasis and drive strong T(h)17/T(h)1 T-cell responses. J Allergy Clin Immunol. 2011;127(3):787-94;e789. https://doi.org/10.1016/j.jaci.2010.12.009.
Krueger JG, Brunner PM. Interleukin-17 alters the biology of many cell types involved in the genesis of psoriasis, systemic inflammation and associated comorbidities. Exper Derm. 2018;27(2):115-23. https://doi.org/10.1111/exd.13467
Tollenaere M, Hebsgaard J, Ewald DA, Lovato P, Garcet S, Li X, et al. Signalling of multiple interleukin (IL)-17 family cytokines via IL-17 receptor A drives psoriasis-related inflammatory pathways. Br J Dermatol. 2021;185(3):585-94. https://doi.org/10.1111/bjd.20090.
Martin DA, Towne JE, Kricorian G, Klekotka P, Gudjonsson JE, Krueger J, et al. The emerging role of interleukin-17 in the pathogenesis of psoriasis: preclinical and clinical findings. J Invest Dermatol. 2013;133(1):17-26. https://doi.org/10.1038/jid.2012.194.
Gerkowicz A, Socha M, Pietrzak A, Zubilewicz T, Krasowska D. The role of VEGF in psoriasis: an update. Acta Angiologica. 2018;24(4):134-40. https://doi.org/10.5603/AA.2018.0019.
Liu C, Liu H, Lu C, Deng J, Yan Y, Chen H, et al. Kaempferol attenuates imiquimod-induced psoriatic skin inflammation in a mouse model. Clin Exper Immun. 2019;198(3):403-15. https://doi.org/10.1111/cei.13363.
Li Y, Cui H, Li S, Li X, Guo H, Nandakumar KS, et al. Kaempferol modulates IFN-? induced JAK-STAT signaling pathway and ameliorates imiquimod-induced psoriasis-like skin lesions. Int Immunopharmacol. 2023;114:109585. https://doi.org/10.1016/j.intimp.2022.109585.
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