This is an outdated version published on 03-06-2024. Read the most recent version.
Forthcoming

Evaluation and characterization of Vachellia leucophloea (Roxb.) Maslin, Seigler & Ebinger using phytochemical, antioxidant, GC-MS, antibacterial, and anticancer properties

Authors

  • S Santhiya Department of Biotechnology, Centre for Bioscience and Nanoscience Research, Eachanari, Coimbatore – 21, Tamilnadu, India
  • Ramachandran Ragunathan Department of Biotechnology, Centre for Bioscience and Nanoscience Research, Eachanari, Coimbatore – 21, Tamilnadu, India https://orcid.org/0000-0003-4416-6232
  • Johney Jesteena Department of Biotechnology, Centre for Bioscience and Nanoscience Research, Eachanari, Coimbatore – 21, Tamilnadu, India https://orcid.org/0000-0002-9932-6005

DOI:

https://doi.org/10.14719/pst.3160

Keywords:

antibacterial, anticancer, antioxidant, GC-MS, Vachellia leucophloea

Abstract

Medicinal plants provide a diverse spectrum of biological compounds that have significant uses in medicine and the well-being of humans. Vachellia leucophloea is a widely recognized medicinal plant in India with a wide range of therapeutic characteristics. This tree has been used in traditional medicine to treat numerous types of ailments since ancient times. Vachellia leucophloea contributes a significant part in regulating the death of cancer cells despite causing no adverse effects. In this present study, the bark of V. leucophloea was subjected to phytochemical analysis, total antioxidant activity by phosphor-molybdenum method, and DPPH assay to detect the radical scavenging activity, total phenol content for determining the plant’s antioxidant activity. Antibacterial and antifungal activities were performed by the agar well diffusion method. UV-Vis, FTIR, GC-MS, and TLC studies were performed to confirm the presence of compounds. The extract was subjected to MIC and TVC studies in which the plant’s ability to inhibit bacterial growth can be determined. DNA fragmentation assay was also analyzed by agarose gel electrophoresis. MTT assay was performed to analyze the anticancer activity against the MCF-7 Cell Line.

Downloads

Download data is not yet available.

References

Bulbul MR, Chowdhury MN, Naima TA, Sami SA, Imtiaj MS, Huda N, Uddin MG. A comprehensive review on the diverse pharmacological perspectives of Terminalia chebula Retz. Heliyon. 2022;8(8): e10220. https://doi.org/10.1016/j.heliyon.2022.e10220

Prusti A, Mishra SR, Sahoo S, Mishra SK. Antibacterial activity of some Indian medicinal plants. Ethnobotanical Leaflets.2008;2008(1):27.

Pandey BP, Adhikari K, Pradhan SP, Shin HJ, Lee EK, Jung HJ. In-vitro antioxidant, anti-cancer, and anti-inflammatory activities of selected medicinal plants from western Nepal. Future Journal of Pharmaceutical Sciences. 2020;6:1-2. https://doi.org/10.1186/s43094-020-00107-0

Sathasivampillai S, Sebastian P. Bioactivities of extracts and isolated compounds of Vachellialeucophloea (Roxb.) Maslin, Seigler and Ebinger. Bulletin of Biotechnology. 2021;2(1):16-22. https://doi.org/10.51539/biotech.948394

Setia A, Sharma M, Nagdev S, Alok S, Mude G, Verma M, Behere S, Golani P and Lodhi DS. Comprehensive study of Acacia leucophloea (Roxb.): traditional uses, phytochemistry and pharmacological significance. International Journal of Pharmaceutical Sciences and Research. 2022; 13(8): 3154-62. http://dx.doi.org/10.13040/IJPSR.0975-8232.13(8).3154-62

Bobade AF. GC-MS and Pharmacognostic Study of Acacia Leucophloealeaves. International Journal of Pharma and Bio Science. 2020;11(3):p1-9. http://dx.doi.org/10.22376/ijpbs.2020.11.3.p1-9

Imran I, Hussain L, Zia-Ul-Haq M, Janbaz KH, Gilani AH, De Feo V. Gastrointestial and respiratory activities of Acacia leucophloea. Journal of Ethnopharmacology. 2011;138(3):676-82. https://doi.org/10.1016/j.jep.2011.09.019

Nelson VK, Sahoo NK, Sahu M, Sudhan HH, Pullaiah CP, Muralikrishna KS. In vitro anticancer activity of Eclipta alba whole plant extract on colon cancer cell HCT-116. BMC Complementary Medicine and Therapies. 2020;20(1):355. https://doi.org/10.1186/s12906-020-03118-9

Mukavi JW, Mayeku PW, Nyaga JM, Kituyi SN. In vitro anti-cancer efficacy and phyto-chemical screening of solvent extracts of Kigelia africana (Lam.) Benth. Heliyon. 2020;6(7). https://doi.org/10.1016/j.heliyon.2020.e04481

Khalil N, Ashour M, Singab AN, Salama O. Bioassay guided fractionation and cytotoxic activity of Daucus carota var. boissieri. Future Journal of Pharmaceutical Sciences. 2018;4(1):14-17. https://doi.org/10.1016/j.fjps.2017.07.002

Mothana RA, Abdo SA, Hasson S, Althawab F, Alaghbari SA, Lindequist U. Antimicrobial, antioxidant and cytotoxic activities and phytochemical screening of some yemeni medicinal plants. Evidence-based Complementary and Alternative medicine. 2010;7(3):323-330. https://doi.org/10.1093/ecam/nen004

Mariswamy Y, Gnaraj WE, Johnson M. Chromatographic finger print analysis of steroids in Aerva lanata L by HPTLC technique. Asian Pacific journal of tropical biomedicine. 2011;1(6), 428-433. https://doi.org/10.1016/S2221-1691(11)60094-4

Devmurari VP. Phytochemical screening study and antibacterial evaluation of Symplocos racemosaRoxb. Archives of Applied Science Research. 2010;2(1), 354-359.

El Jemli M, Kamal R, Marmouzi I, Zerrouki A, Cherrah Y, Alaoui K.Radical-Scavenging Activity and Ferric Reducing Ability of Juniperus thurifera (L.), J. oxycedrus (L.), J. phoenicea (L.) and Tetraclinis articulata (L.). Advances in Pharmacological and Pharmaceutical Sciences.2016; 2016. https://doi.org/10.1155/2016/6392656

Jaya Prakash MA, R. Ragunathan, Jesteena Johney. Evaluation of bioactive compounds from Jasminum polyanthum and its medicinal properties. Journal of drug delivery and therapeutics. 2019; 9(2): 303-310. https://doi.org/10.22270/jddt.v9i2.2413

Cavazos P, Gonzalez D, Lanorio J, Ynalvez R. Secondary metabolites, antibacterial and antioxidant properties of the leaf extracts of Acacia rigidula benth. and Acacia berlandieri benth. SN Applied Sciences. 2021;3:1-14. https://doi.org/10.1007/s42452-021-04513-8

Lakshmi T, Ramasamy R, Thirumalaikumaran R. Preliminary phytochemical analysis and in vitro antioxidant, FTIR spectroscopy, anti-diabetic activity of Acacia catechu ethanolic seed extract. Pharmacognosy Journal. 2015;7(6):356-362. http://dx.doi.org/10.5530/pj.2015.6.7

Ahamed T, Rahman SM, Shohael AM. Thin layer chromatographic profiling and phytochemical screening of six medicinal plants in Bangladesh. International Journal of Biosciences. 2017;11(1):131-140. http://dx.doi.org/10.12692/ijb/11.1.131-140

Jesteena J, Radhai SS, Ragunathan R. Extraction of Chitin and Chitosan from Wild Type Pleurotus Spp and its Potential Application – Innovative Approach. Journal of Pure and Applied Microbiology. 2018; 12(3): 1631-1640. https://doi.org/10.22207/JPAM.12.3.70

Camporese A, Balick M, Arvigo R, Esposito R, Morsellino N, De Simone F, Tubaro A. Screening of anti-bacterial activity of medicinal plants from Belize (central America). J Ethnopharmacol. 2003;87(1):103–107. https://doi.org/10.1016/S0378-8741(03)00115-6

Jesteena J, Radhai SS, Ragunathan R. Development of Chitosan Silver Nanocomposites: Its Characteristic Study and Toxicity Effect against 3T3-L1 Cell Line. Journal of Pure and Applied Microbiology. 2022;16(1):494-502. https://doi.org/10.22207/JPAM.16.1.47

Gulbagca F, Ozdemir S, Gulcan M, Sen F. Synthesis and characterization of Rosa canina-mediated biogenic silver nanoparticles for anti-oxidant, antibacterial, antifungal, and DNA cleavage activities.Heliyon. 2019;5(12). https://doi.org/10.1016/j.heliyon.2019.e02980

Wankhade MS, Mulani RM. Preliminary phytochemical screening and chromatographic finerprint analysis of Acacia leucopholea roxb. methanolic leaf and bark extract by HPTLC technique. Indo-American Journal of Pharmaceutical Research. 2015;4(1):184-91.

Jain S, Sharma P, Jhade D, Sharma N, Paliwal P, Ahirwar D. Pharmacognostic screening and phytochemical evaluation of Acacia leucophloea root. International Journal of Green Pharmacy. 2011;5(2):155. http://dx.doi.org/10.4103/0973-8258.85183

Sowndhararajan K, Joseph JM, Manian S. Antioxidant and free radical scavenging activities of Indian Acacias: Acacia leucophloea (Roxb.) Willd., Acacia ferruginea DC., Acacia dealbata Link. and Acacia pennata (L.) Willd. International Journal of Food Properties. 2013;16(8):1717-29. https://doi.org/10.1080/10942912.2011.604895

Sulaiman C, Gopalakrishnan V, Balachandran I. Chromatographic and spectrophotometric studies in selected Acacia species. JPC-Journal of Planar Chromatography-Modern TLC. 2014;27(2):88-92. https://doi.org/10.1556/JPC.27.2014.2.3

Alapati KS, Padmavathi CH, Sowjanya KM, Suman Joshi DSD, Rathnakar Reddi KVN, Swathi J, Narendra K. Evaluation of In-Vitro Anti-Oxidant, Anti- Microbial Activities and Screening of Medically Active Chemical Components of Acacia leucophloea Leaf Extracts. American Journal of Pharmacy and Health Research 2014;2(11).

Dabur R, Gupta A, Mandal TK, Singh DD, Bajpai V, Gurav AM, Lavekar GS. Antimicrobial activity of some Indian medicinal plants. African Journal of Traditional, Complementary and Alternative Medicines. 2007;4(3):313-318. https://doi.org/10.4314/ajtcam.v4i3.31225

Afsar T, Razak S, Khan MR, Mawash S, Almajwal A, Shabir M, Haq IU. Evaluation of antioxidant, anti-hemolytic, and anticancer activity of various solvent extracts of Acacia hydaspica R. Parker aerial parts. BMC complementary and Alternative medicine. 2016;16(1):1-6. https://doi.org/10.1186/s12906-016-1240-8

Mohamed EH, Alghamdi YS, Mostafa Abdel-Hafez S, Soliman MM, Alotaibi SH, Hassan MY, Hany NA, Amer HH. Susceptibility assessment of multidrug-resistant bacteria to natural products. Dose-Response. 2020;18(3):1559325820936189. https://doi.org/10.1177/1559325820936189

Published

03-06-2024

Versions

How to Cite

1.
Santhiya S, Ragunathan R, Jesteena J. Evaluation and characterization of Vachellia leucophloea (Roxb.) Maslin, Seigler & Ebinger using phytochemical, antioxidant, GC-MS, antibacterial, and anticancer properties. Plant Sci. Today [Internet]. 2024 Jun. 3 [cited 2024 Nov. 21];. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/3160

Issue

Section

Special issue on Ethnobotany