This study presents a green synthesis approach for silver nanoparticles (AgNPs) using an aqueous leaf extract of Aegle marmelos. This approach provides AgNPs with median size of 21 nm and plasmon resonance structures with a peak at 474 nm. The synthesized AgNPs were characterized using SEM EDX, FTIR, XRD and UV-Vis Spectrometer. The biosynthesis of AgNPs demonstrated notable antibacterial activity, with the highest zone of inhibition observed for E. coli (16.23 ± 0.87 mm) at a concentration of 150 µg/mL. The AgNPs were further evaluated for their anticancer activity against HCT 116 cell lines and it was declared that the AgNPs were active against the cell lines with an IC50 value of 39.33 ± 0.19 µg/mL. These findings suggest that AgNPs synthesised using A. marmelos extract possess promising antimicrobial and anticancer properties.