Preliminary phytochemical characterization and in vitro antioxidant and antimicrobial potential screening of an ayurvedic drug combination Panchatikta

Authors

DOI:

https://doi.org/10.14719/pst.3481

Keywords:

Panchatikta, HPTLC, DPPH, ABTS, Antioxidant, Antimicrobial, Flavonoid, Phenol, Ayurveda

Abstract

Panchatikta is an Ayurvedic drug combination of 5 bitter-tasting drugs, namely Azadirachta indica, Tinospora cordifolia, Justicia adhatoda, Solanum virginianum and Trichosanthes dioica. In this study, estimations of the phenolic, flavonoids and tannin contents, HPTLC profile and in vitro anti-oxidant and anti-microbial potential of 3 extracts of the Panchatikta were carried out. The total phenolic content of the Panchatikta combination was almost the same in ethanol, water and hydroalcoholic extracts. Flavonoid content and tannin content were highest in ethanol extract. HPTLC profiling of the 3 extracts of Panchatikta showed the maximum number of peaks for the ethanolic extract. Water extract showed a comparatively smaller number of peaks. The maximum number of peaks was seen after derivatisation. In the DPPH assay, gallic acid has IC50 value of 5.50 ± 0.38 µg/mL; ethanol and hydroalcoholic extracts have IC50 close to 60 µg/mL and water extract has around 90 µg/mL. The ABTS assay showed standard ascorbic acid has a IC50 value of 67.33 ± 0.58 µg/mL while Panchatikta extracts have a much higher IC50. In nitric oxide assay also, tested samples have a higher IC50 when compared with that of ascorbic acid (177.33 ± 5.09). In well diffusion assay for antibacterial screening, all 3 extracts have a significant zone of inhibition at concentrations of 500 µg and 1000 µg. Pseudomonas aeruginosa was found to be resistant to all 3 test samples and standard antibiotic discs. Minimum activity was obtained against Klebsiella pneumonia. Panchatikta extracts at concentrations of 500 µg and 1000 µg showed significant activity against the fungal strains Aspergillus niger and Fusarium oxysporum. In conclusion, the results of the study can provide scientific support for the traditional usage of the Ayurvedic drug combination Panchatikta for various ailments.

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References

Karole S, Shrivastava S, Thomas S, Soni B, Khan S, Dubey J et al. Polyherbal formulation concept for synergic action: A review. Journal of Drug Delivery and Therapeutics. 2019;9(1):453-66. http://dx.doi.org/10.22270/jddt.v9i1-s.2339

Harinarayanan CM, Kumar D, Geetha SP, Kumar A. The phytochemical composition and therapeutic efficacy of an ayurvedic drug combination Panchatikta: A review. Medicinal Plants-International Journal of Phytomedicines and Related Industries. 2022;14(3):355-73. http://dx.doi.org/10.5958/0975-6892.2022.00041.7

Sulaiman CT, Balachandran I. Total phenolics and total flavonoids in selected Indian medicinal plants. Indian Journal of Pharmaceutical Sciences. 2012;74(3):258-60. http://dx.doi.org/10.4103/0250-474X.106069

Slinkard K, Singleton VL. Total phenol analysis: Automation and comparison with manual methods. American Journal of Enology and Viticulture. 1977;28(1):49-55. https://doi.org/10.5344/ajev.1974.28.1.49

Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 2002;10(3). https://doi.org/10.38212/2224-6614.2748

Anonymous. The ayurvedic pharmacopoeia of India. Part I. 1st ed. VI. New Delhi: Ministry of Health and Family Welfare, Department of AYUSH, Govt. of India. 2008; p. 336.

Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;181(4617):1199-200. https://doi.org/10.1038/1811199a0

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 1999;26(9-10):1231-37. https://doi.org/10.1016/S0891-5849(98)00315-3

Ebrahimzadeh MA, Pourmorad F, Hafezi S. Antioxidant activities of Iranian corn silk. Turkish Journal of biology. 2008;32(1):43-49.

Parez CP, Bezerque MP. An antibiotic assay by the agar-well diffusion method. Acta biologiae et medicinae experimentalis. 1990;15-13.

Mukherjee PK, Harwansh RK, Bahadur S, Banerjee S, Kar A. Evidence based validation of Indian traditional medicine–way forward. World Journal of Traditional Chinese Medicine. 2016 Jan 1;2(1):48-61. https://doi.org/10.1142/9789813200340_0007

Pérez-Jiménez J, Neveu V, Vos F, Scalbert A. Identification of the 100 richest dietary sources of polyphenols: An application of the phenol-explorer database. European Journal of Clinical Nutrition. 2010;64(3):S112-20. https://doi.org/10.1038/ejcn.2010.221

Johari MA, Khong HY. Total phenolic content and antioxidant and antibacterial activities of Pereskia bleo. Advances in Pharmacological and Pharmaceutical Sciences. 2019;1-4. https://doi.org/10.1155/2019/7428593

Islas JF, Acosta E, Zuca G, Delgado-Gallegos JL, Moreno-Treviño MG, Escalante B, Moreno-Cuevas JE. An overview of Neem (Azadirachta indica) and its potential impact on health. Journal of Functional Foods. 2020;74:104171. https://doi.org/10.1016/j.jff.2020.104171

Royani A, Hanafi M, Lotulung PD, Prastya ME, Verma C, Alfantazi A, Manaf A. Parameter processes and their impact on Tinospora cordifolia stem extracts for antibacterial and antioxidant materials. Materials Today: Proceedings. 2023. https://doi.org/10.1111/jfpp.15772

Rohilla P, Chhikara A, Dahiya P. Phytochemical screening, in vitro anti-bacterial and antioxidant efficacy of Solanum virginianum L. aerial vegetative parts extracted in four solvents. Pharmacognosy Research. 2023;15(4):796-804. http://dx.doi.org/10.5530/pres.15.4.084

Khatua S, Pandey A, Biswas SJ. Phytochemical evaluation and antimicrobial properties of Trichosanthes dioica root extract. Journal of Pharmacognosy and Phytochemistry. 2016;5(5):410-13.

Tourabi M, Metouekel A, Ghouizi AE, Jeddi M, Nouioura G, Laaroussi H et al. Efficacy of various extracting solvents on phytochemical composition and biological properties of Mentha longifolia L. leaf extracts. Scientific Reports. 2023;13(1):18028. https://doi.org/10.1038/s41598-023-45030-5

Jethava N, Toshikhane H, Bhatt D. Healing potential of Pantchtikta Ghrita in the management of post drainage wound of Psoas Abscess-A rare case report. International Journal of AYUSH Case Reports. 2017;1(1):19-25.

Published

24-05-2024 — Updated on 01-06-2024

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How to Cite

1.
CM H, M D, SP G, Kumar A. Preliminary phytochemical characterization and in vitro antioxidant and antimicrobial potential screening of an ayurvedic drug combination Panchatikta. Plant Sci. Today [Internet]. 2024 Jun. 1 [cited 2024 Nov. 23];11(2). Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/3481

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Research Articles