Enhancing biotechnological approaches for the in vitro micropropagation: Protecting endangered wild tulip species in Samarkand, Uzbekistan

Authors

DOI:

https://doi.org/10.14719/pst.3653

Keywords:

bulb рrораgаtiоn, сulturе mеdium, in-vitrо, miсrосlоnаl рrораgаtiоn, sееd рrораgаtiоn, Tuliра

Abstract

Tulipa, a genus of bulbous geophytes, boasts a globally recognized ornamental value. Anthropogenic influences have precipitated a decline in the wild populations of Tulipa, including those in Uzbekistan, necessitating the development of forward-looking conservation strategies. This study focuses on advancing biotechnological strategies for in vitro micropropagation to conserve endangered wild Tulipa species in Samarkand, Uzbekistan. Tulipa ingens and Tulipa fosteriana, listed as vulnerable and near threatened, respectively, on the IUCN Red List, face numerous anthropogenic threats including habitat loss and overexploitation. Our research demonstrates the effectiveness of sterilization protocols, successful micropropagation from seeds, and the importance of adapting to non-sterile conditions. Through meticulous experimentation, a novel sterilization approach involving a 5 percent Domestos solution coupled with brief ethanol exposure yielded the highest viability rates for plant materials.
Micropropagation from seeds resulted in successful shoot and microbulb formation, providing a promising conservation strategy for these rare species. The study underscores the urgency of conservation efforts in Uzbekistan, where the extent of occurrence and area of occupancy of these species classify them as endangered. Our findings contribute valuable data for ex-situ conservation measures, offering hope for the preservation of red-listed Tulipa species in the region.

Downloads

Download data is not yet available.

References

Christenhusz M, Govaerts R, David J, Hall T, Borland K, Roberts P et al. Tiptoe through the tulips – Cultural history, molecular phylogenetics and classification of Tulipa (Liliaceae). Bot J Linn Soc. 2013;172. https://doi.org/10.1111/boj.12061

Hajdari A, Pulaj B, Schmiderer C, Mala X, Wilson B, Lluga-Rizani K, Mustafa B. A phylogenetic analysis of the wild Tulipa species (Liliaceae) of Kosovo based on plastid and nuclear DNA sequence. Adv Genet (Hoboken). 2021;2(3). https://doi.org/10.1002/ggn2.202100016

WFO. Tulipa. World Flora Online; 2023. https://www.worldfloraonline.org/

Asatulloev T, Dekhkonov D, Tojibaev K. What determines the distribution of Tulipa species in Uzbekistan? Plant Divers Cent Asia. 2022;1:128-37. https://doi.org/10.54981/PDCA/vol1_iss2/a5

Dekhkonov D, Tojibaev K, Makhmudjanov D, Nuree N, Baasanmunkh S, Yusupov Z et al. Mapping and analyzing the distribution of the species in the genus Tulipa (Liliaceae) in the Ferghana valley of Central Asia. Korean J Plant Taxon. 2021;51:181-91. https://doi.org/10.11110/kjpt.2021.51.3.181

Tojibaev K, Dekhkonov D, Ergashov I, Sun H, Deng T, Yusupov Z. The synopsis of the genus Tulipa (Liliaceae) in Uzbekistan. Phytotaxa. 2022;573:163-214. https://doi.org/10.11646/phytotaxa.573.2.2

Botschantzeva ZP, Varenkamp HQ. Tulips: Taxonomy, morphology, cytology, phytogeography and physiology. Taxon. 1982;31:779. https://doi.org/10.2307/1219713

Dekhkonov D, Temur A, Tojiboeva U, Sar? ?, Tojibaev K. Suitable habitat prediction with a huge set of variables on some Central Asian tulips. 2022;16. https://doi.org/10.1016/j.japb.2022.10.007

Everett D, Fay MF, Christenhusz MJM, Wilford R, Royal Botanic Gardens K. The genus Tulipa: Tulips of the World. Kew Publishing. 2013. https://books.google.co.id/books?id=ga60naeacaaj

Shukrullozoda R, D?khk?n?v D, Kh?yd?r?v Kh, Kadirov B, Tojibaev K. Morphology and distribution patterns of Tulipa fosteriana and Tulipa ingens. Plant Sci Today. 2023;10(2):426-38. https://doi.org/10.14719/pst.2296

Shukrullo Qizi SR, Burxonovich DD, Kudratovich KK, Iskandarovna UZ, Gùlomjon Qizi OS, Komiljon Qizi NG. Analysis of the distribution of Tulipa fosteriana and Tulipa ingens. The importance of protecting wild plants, in particular tulips all over the World. Am J Plant Sci. 2023;14:613-24. https://doi.org/10.4236/ajps.2023.145041

Tojibaev K, Beshko N. Reassessment of diversity and analysis of distribution in Tulipa (Liliaceae) in Uzbekistan. Nord J Bot. 2014;33. https://doi.org/10.1111/njb.00616

Wilson B. Tulipa: The taxonomy and evolutionary history of the genus and its impact on conservation priorities in Central Asia [University of Cambridge]. 2023.

Shukrull?z?d? R, D?khk?n?v D, Kh?yd?r?v ?. Optimization of sterilization process and culture medium for the micropropagation of Tulipa fosteriana and Tulipa ingens. Sci Bull Namangan State Univ. 2022;8:103-10.

IUCN. Red list of threatened species. International Union for Conservation of Nature. 2023. https://www.iucnredlist.org/

Sennikov A, Tojibaev K, Khassanov F, Beshko N. The flora of Uzbekistan project. Phytotaxa. 2016;282:107-18. https://doi.org/10.11646/phytotaxa.282.2.2

Khasanov F. The red data book of the republic of Uzbekistan. Chinor Enk, Tashkent. 2019;360 pp.

Dekhkonov D, Asatulloev T, Akbarov F, Umida T, Lorenzo P, Tojibaev K. Conservation strategy and diversity of Tulipa (Liliaceae) in Uzbekistan. Plant Divers Cent Asia. 2023;4:1-42. https://doi.org/10.54981/PDCA/vol2_iss2/a2

Alderson PG, Rice RD, Wright NA. Towards the propagation of Tulip in vitro. Acta Horticulturae. 1983;131:39-48. https://doi.org/10.17660/ActaHortic.1983.131.3

Bhat M, Fayaz M, Kumar A, Fayaz M, Najar R, Anjum M, Jain A. Micropropagation of Tulipa species. 2020;39-58. https://doi.org/10.1201/9781003000723-3

Kuijpers AM, Langens-Gerrits M. Propagation of tulip in vitro. Acta Horticulturae. 1997;430:321-24. https://doi.org/10.17660/ActaHortic.1997.430.49

Minas GJ. In vitro propagation of akama tulip via adventitious organogenesis from bulb slices. Acta Horticulturae. 2007;313-16. https://doi.org/10.17660/ActaHortic.2007.755.40

Nishiuchi Y. Studies on vegetative propagation of Tulip. Jpn Soc Hort Sci. 1976;45:59-64. https://doi.org/10.2503/jjshs.45.59

Podwyszy?ska M, Novak O, Doležal K, Miroslav S. Endogenous cytokinin dynamics in micropropagated tulips during bulb formation process influenced by TDZ and iP pretreatment. Plant Cell Tissue Organ Cult. 2014;119:331-46. https://doi.org/10.1007/s11240-014-0537-x

Bapat V, Yadav S, Dixit GB. Rescue of endangered plants through biotechnological applications. Nat Acad Sci Lett. 2008;31:201-10.

Chokheli VA, Dmitriev PA, Rajput VD, Bakulin SD, Azarov AS, Varduni TV et al. Recent development in micropropagation techniques for rare plant species. Plants. 2020;9(12). https://doi.org/10.3390/plants9121733

Hasnain A, Naqvi SAH, Ayesha SI, Khalid F, Ellahi M, Iqbal S et al. Plants in vitro propagation with its applications in food, pharmaceuticals and cosmetic industries; Current scenario and future approaches. Front Plant Sci. 2022;13:1009395. https://doi.org/10.3389/fpls.2022.1009395

Pence VC. The possibilities and challenges of in vitro methods for plant conservation. Kew Bull. 2010;65(4):539-47. http://www.jstor.org/stable/23044620

Akhmetova AS. Introduksiya i razmnojeniye tyulpanov in vivo i in vitro v lesostepnoy zone. Bashkirskogo Preduralya [Doctoral dissertation]. Orenburg; 2009.

Z?its?v?, Y. G. (2015). ?s?b?nn?sti m?rf?g?n?z? i r?zmn?j?niy? in vitr? n?k?t?ri? ?r?dst?vit?l?y r?d? Rh?d?d?ndr?n L. N?v?sibirsk. Timof??v? OA, N?vm?rzhitsk?y? YY. Uchebno metodicheskoye posobiye [Kazanskiy universitet]. Kazan; 2012.

Mukhametvafina AA. Introduksiya liliy v Bashkirskom Preduralye i ikh razmnojeniye in vivo i in vitro [UFA]. Ufa; 2009.

Wright NA, Alderson PG. The growth of tulip tissue. Acta Horticulturae. 1980;109:263-70. https://doi.org/10.17660/ActaHortic.1980.109.37

Zonneveld BJM. 2009. The systematic value of nuclear genome size for “all” species of Tulipa L. (Liliaceae). Plant Systematics and Evolution, 281(1-4), 217-245. https://doi.org/10.1007/s00606-009-0203-7

Ostertagova E, Ostertag O, Ková? J. Methodology and application of the Kruskal-Wallis test. Appl Mech Mater. 2014;611:115-22. https://doi.org/10.4028/www.scientific.net/AMM.611.115

Gurel S, Topal E, and Ekrem G. 2014. The Effect of Pretreating Seedlings with TDZ on Direct Shoot Regeneration from Petiole Explants of Sugar Beet (Beta vulgaris L.). Biotechnology & Biotechnological Equipment, 17. https://doi.org/10.1080/13102818.2003.10817064

Aabed K, Mohammed AE. Synergistic and antagonistic effects of biogenic silver nanoparticles in combination with antibiotics against some pathogenic microbes. Front Bioeng Biotechnol. 2021;9:652362. https://doi.org/10.3389/fbioe.2021.652362

Sosnowski J, Truba M, Vasileva V. The impact of auxin and cytokinin on the growth and development of selected crops. Agriculture. 2023;13(3):724. https://www.mdpi.com/2077-0472/13/3/724

Abdalla N, El-Ramady H, Seliem MK, El-Mahrouk ME, Taha N, Bayoumi Y et al. An academic and technical overview on plant micropropagation challenges. Horticulturae. 2022;8(8). https://doi.org/10.3390/horticulturae8080677

Dolce N, Medina R, Terada G, González-Arnao M, Flachsland E. In vitro propagation and germplasm conservation of wild orchids from South America. 2020;37-94. https://doi.org/10.1007/978-981-32-9456-1_4

Giusti P, Vitti D, Fiocchetti F, Colla G, Saccardo F, Tucci M. In vitro propagation of three endangered cactus species. Scientia Horticulturae. 2002;95(4):319-32. https://doi.org/10.1016/S0304-4238(02)00031-6

Published

10-06-2024 — Updated on 10-06-2024

Versions

How to Cite

1.
Shukrullozoda R, Kadirov B, Khaydarov K, Dekhkonov D, Umurzakova Z, Ruziev F, Matkarimova G, Rajabov A, Mavlanov K, Safin M, Tursunbоеv K. Enhancing biotechnological approaches for the in vitro micropropagation: Protecting endangered wild tulip species in Samarkand, Uzbekistan. Plant Sci. Today [Internet]. 2024 Jun. 10 [cited 2024 Nov. 21];11(2). Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/3653

Issue

Section

Research Articles

Most read articles by the same author(s)

Similar Articles

You may also start an advanced similarity search for this article.