Chemical composition and antioxidant activity of Physalis angulata L. (Solanaceae) and its effect on blood clotting and biofilm formation of some wound bacterial isolates
DOI:
https://doi.org/10.14719/pst.3898Keywords:
Antioxidant activity, biofilm formation, blood coagulation management, chemical composition, Physalis angulata LAbstract
Physalis angulata a member of the Solanaceae family, is a plant recognized for its significant nutritional and medicinal properties. Widely distributed across tropical and subtropical regions, this plant has garnered attention for its diverse biological activities. The ethanol extract from the plant is being used in this study to investigate its biological and antioxidant properties. The ability of the extract to reduce free radicals was also tested using the compound phenyl-1-picrylhydrazyl). The DPPH method was used because it is a modern color method and is considered the most common and easy to apply, in addition to its speed and superior sensitivity in testing antioxidants for samples of plant extracts, and the values were compared with the value of ascorbic acid IC50. The study also looks at the extract's ability to prevent the formation of biofilms by different pathogens, including E. coli and Staphylococcus strains that were isolated from community labs and Ramadi Teaching Hospital. The ethanol extract demonstrated a noteworthy ability to inhibit the formation of cellular membranes in staphylococcal bacteria and E. coli. The efficacy of the extract at different concentrations (150, 200, 250 mg/mL) was also assessed, revealing that a concentration of 250 mg/mL resulted in significant inhibition rates (74.3% and 61.45% for E. coli). Furthermore, the study explores the impact of ethanol extract on blood coagulation. At high concentrations (150, 200, 250 mg/mL), the extract exhibited properties akin to aspirin and warfarin, extending the clotting times in both total and partial coagulation tests (Prothrombin time, Partial thromboplastin time) at lower concentrations (100, 50, 10 mg/mL), the extract appeared to promote blood clot formation, suggesting a concentration-dependent effect on hemostasis. This dual action positions P.angulate as a potential therapeutic agent, warranting further investigation into its biomedical applications.
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Kasali FM, Tusiimire J, Kadima JN, Tolo CU, Weisheit A, Agaba AG. Ethnotherapeutic uses and phytochemical composition of Physalis peruviana L.: an overview. Sci World J. 2021; 2021. https://doi.org/10.1155/2021/5212348
Mohammed Hasan ZY. Phytochemical investigation and antioxidant activity of total phenols in the aerial parts of some Asteraceae family wild plants grown in western of Iraq. Syst Rev Pharm. 2020; 11(1).
Ferreira LM dos SL, Vale AE do, Souza AJ de, Leite KB, Sacramento C, Moreno MLV, et al. Anatomical and phytochemical characterization of Physalis angulata L.: A plant with therapeutic potential. 2019; 11(2):171-177. https://doi.org/10.4103/pr.pr_97_18
de Oliveira AM, Malunga LN, Perussello CA, Beta T, Ribani RH. Phenolic acids from fruits of Physalis angulata L. in two stages of maturation. South African J Bot. 2020; 131:448-53. https://doi.org/10.1016/j.sajb.2020.02.029
Al Rajab ATH, Khalaf HY. The activity of ethanolic extract of Capsella bursa on some biochemical parameters of mice. Biochem Cell Arch. 2019; 19(1).
Ramakrishna Pillai J, Wali AF, Menezes GA, Rehman MU, Wani TA, Arafah A, et al. Chemical composition analysis, cytotoxic, antimicrobial and antioxidant activities of Physalis angulata L.: A comparative study of leaves and fruit. Molecules. 2022; 27(5):1480. https://doi.org/10.3390/molecules27051480
Shenstone E, Lippman Z, Van Eck J. A review of nutritional properties and health benefits of Physalis species. Plant Foods Hum Nutr. 2020;75:316-25. https://doi.org/10.1007/s11130-020-00821-3
Hu S, Li X, Yin X, Li R, Zhang R, Zang J, et al. Species-specific identification of Pseudomonas based on 16S–23S rRNA gene internal transcribed spacer (ITS) and its combined application with next-generation sequencing. BMC Microbiol. 2022;22(1):188. https://doi.org/10.1186/s12866-022-02607-w
Sambyal SS, Sharma P, Shrivastava D. Anti-biofilm activity of selected plant essential oils against Pseudomonas aeruginosa and Staphylococcus aureus. Int J Curr Microbiol Appl Sci. 2017;6(3). https://doi.org/10.20546/ijcmas.2017.603.051
Farhan HN, Hameed AT, Aobad HM. The biological activity of some Pseudomonas sp. isolates on the growth of three plant pathogenic fungi under incubator conditions. Adv Environ Biol. 2010;4(1):53-7.
Osho A, Adetunji T, Fayemi SO, Moronkola DP. Antimicrobial activity of essential oils of Physalis angulata L. African J Tradit Complement Altern Med. 2010;7(4). https://doi.org/10.4314/ajtcam.v7i4.56696
Silva MTG, Simas SM, Batista TGFM, Cardarelli P, Tomassini TCB. Studies on antimicrobial activity, in vitro, of Physalis angulata L. (Solanaceae) fraction and physalin B bringing out the importance of assay determination. Mem Inst Oswaldo Cruz. 2005;100:779-82. https://doi.org/10.1590/S0074-02762005000700018
Jilbhkate YJ, Awachat AP, Lohiya RT, Umekar MJ, Hemke AT, Gupta KR. Extraction: An important tool in the pharmaceutical field. Int J Sci Res Arch. 2023;10(1):555-68. https://doi.org/10.30574/ijsra.2023.10.1.0768
Adámez JD, Samino EG, Sánchez EV, González-Gómez D. In vitro estimation of the antibacterial activity and antioxidant capacity of aqueous extracts from grape-seeds (Vitis vinifera L.). Food Control. 2012;24(1–2):136-41. https://doi.org/10.1016/j.foodcont.2011.09.016
Gunendren M, Noordin SS, Muggundha R, Nozlena AS. Effect of Ocimum sanctum (Tulsi) aqueous leaf extract on prothrombin time (PT), activated partial thromboplastin time (APTT), and thrombin time (TT) of human plasma. J Biomed Clin Sci. 2017;2(1).
Rutter WC, Burgess DR, Burgess DS. Increasing incidence of multidrug resistance among cystic fibrosis respiratory bacterial isolates. Microb drug Resist. 2017;23(1):51-5. https://doi.org/10.1089/mdr.2016.0048
Dahal RH, Chaudhary DK. Microbial infections and antimicrobial resistance in Nepal: current trends and recommendations. Open Microbiol J. 2018;12:230. https://doi.org/10.2174/1874285801812010230
Gudiña EJ, Rocha V, Teixeira JA, Rodrigues LR. Antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp. paracasei A20. Lett Appl Microbiol. 2010;50(4):419-24. https://doi.org/10.1111/j.1472-765X.2010.02818.x
Steel RGD, Torrie JH. Principles and procedures of statistics. Princ Proced Stat. 1960;
Chou MY, Andersen TB, Mechan Llontop ME, Beculheimer N, Sow A, Moreno N, et al. Terpenes modulate bacterial and fungal growth and sorghum rhizobiome communities. Microbiol Spectr. 2023;11(5):e01332-23. https://doi.org/10.1128/spectrum.01332-23
Amer AA, Mohammed RS, Hussein Y, Ali ASM, Khalil AA. Development of Lepidium sativum extracts/PVA electrospun nanofibers as wound healing dressing. ACS omega. 2022;7(24):20683-95. https://doi.org/10.1021/acsomega.2c00912
Lee CY, Sharma A, Semenya J, Anamoah C, Chapman KN, Barone V. Computational study of ortho-substituent effects on antioxidant activities of phenolic dendritic antioxidants. Antioxidants. 2020;9(3):189. https://doi.org/10.3390/antiox9030189
Omar G, Abdallah L, Barakat A, Othman R, Bourinee H. Eficácia hemostática in vitro de extratos aquosos, metanólicos e etanólicos de três espécies de plantas medicinais na Palestina. Brazilian J Biol. 2019;80:763-8. https://doi.org/10.1590/1519-6984.219186
Mengome LE, Voxeur A, Akue JP, Lerouge P, Ngouamizokou A, Moutsimbi RM. Analysis of toxicity and hemostatic properties of polysaccharides from plants endemic to Gabon. Br J Pharmacol Toxicol. 2014;5(6):186-93. https://doi.org/10.19026/bjpt.5.5185
Alabdallat NG, Dukhyil AAA Bin. In vitro anticoagulant effect of methanolic extracts of Artemisia herba-alba, Achillea fragrantissima and Citrullus colocynthis grown in Saudi Arabia. 2021;
Stiefel P, Rosenberg U, Schneider J, Mauerhofer S, Maniura-Weber K, Ren Q. Is biofilm removal properly assessed? Comparison of different quantification methods in a 96-well plate system. Appl Microbiol Biotechnol. 2016;100:4135-45. https://doi.org/10.1007/s00253-016-7396-9
Namasivayam SKR, Vivek JM. Screening of quorum sensing (qs) modulatory effect of medicinal plant extracts against quorum sensing mediated virulence factors of human pathogenic gram-negative bacteria. Int J Pharm Phytochem Res. 2016;8:263.
Álvarez-Martínez FJ, Barrajón-Catalán E, Encinar JA, Rodríguez-Díaz JC, Micol V. Antimicrobial capacity of plant polyphenols against gram-positive bacteria: A comprehensive review. Curr Med Chem. 2020;27(15):2576-606. https://doi.org/10.2174/0929867325666181008115650
Jassim RA, Hamid AT. Molecular Characterization of Selected Genera of the (Apiaceae) Family using SSR Molecular Markers. Indian J Forensic Med Toxicol. 2020;14(4).
Mohammed IH, Hameed AT, Salman HF. Phytochemical and biological of Anthemis nobilis (Asteraceae family) a native herb of Iraq. Sys Rev Pharm. 2020;11(2):458-61.
Ushie OA, Neji PA, Abeng FE, Okpashi VE, Baba NH, Azuaga TI. Phytochemical Screening and Antimicrobial Activities of Chloroform and Ethyl Acetate Extracts of Physalis angulata. J Chem Soc Niger. 2019;44(6).
Al-Joboury KR, Aliwy SA. Survey With Revised Checklist of Compositae in The Herbarium of Iraq Natural History Research Center and Museum. Bull Iraq Nat Hist Museum. 2023;17(3):375-407. https://doi.org/10.26842/binhm.7.2023.17.3.0375
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