Optimization of culture conditions for extracellular fungal lipase production by submerged fermentation process
DOI:
https://doi.org/10.14719/pst.2018.5.3.399Keywords:
Carbon sources, fungal isolate, lipases, optimizationAbstract
The present study aimed to optimize culture conditions for optimal growth and production of extracellular lipase. Lipolytic fungal strain named as S3St2 previously isolated from a petrol pump soil sample of Newai Town was used for optimization study. Among the tested carbohydrate carbon sources, polysaccharide-starch exhibited maximum lipase production (21.25±0.70 IU/ml/min) with highest specific activity (1.47±0.06 U/mg). Lipase activity and specific activity were higher with mustard oil 1 % (v/v) among all lipidic carbon sources. Among inorganic nitrogen source, potassium nitrate was found best inducer of lipase activity, malt extract supported the fungus growth (dry weight of cell pellets was 0.467 g) and exhibited maximum lipase activity among all organic nitrogen sources. Lipase activity was optimum at pH 8.0, indicates alkalophillic nature of production media supports the growth of fungus. Higher lipase activity (27.92±0.87 IU/ml/min) was detected at 28ºC. The incubation time of 5 days was found optimum for maximum lipase production (31.51±0.21 IU/ml/min).
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References
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21 Joshi GK, Kumar S, Tripathi BN, Sharma V. Production of alkaline lipase by Corynebacterium paurometabolum MTCC6841 isolated from Lake Naukuchiatal, Uttaranchal State, India. Current Microbiology. 2006; 52(5): 354-358. https://doi.org/10.1007/s00284-005-0224-6
22 Lowry OH, Rosenbrough NJ, Farr AL, Randall A. Protein measurement with the folin phenol reagent. Journal of Biological Chemistry. 1951; 193(1): 265-275.
23 Golani M, Hajela K, Pandey GP. Screening, Identification, Characterization and Production of Bacterial Lipase from Oil Spilled Soil. International Journal of Current Microbiology and Applied Sciences 2013; 5(3): 745-763. http://dx.doi.org/10.20546/ijcmas.2016.503.08
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25 Sneha PM, Parimala T, Rao CSVR, Satish BR. Effect of various sources and parameters on the production of an extracellular lipase from Aspergillus heteromorphus and optimization using response surface methodology. Journal of Chemical, Biological and Physical Sciences. 2012; 2(1): 212-222.
26 Maia MDMD, Morais MMCD, Morais MAD, Melo EHM, Filho JLDL. Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FSI, Revista de Microbiologia. 1999; 30: 304-309.
27 Fan Y, Shang L, Qian J. Screening, identifying and medium optimization of a lipase producing filamentous fungus from soil for high chiral resolution of 1- phenylethanol. African Journal of Microbiology Research. 2003; 7(33): 4235-4243.
28 Bokhari DN, Gohar UF, Hussain Z. Optimization Studies of Lipase Production from Locally isolated Bacillus sp. Biologia (Pakistan). 2013; 59(2): 259-265.
29 Sethi BK, Rout JR, Das R, Nanda PK, Sahoo SL. Lipase production by Aspergillus terreus using mustard seed oil cake as a carbon source. Annals of Microbiology. 2013; 63(1): 241-252. DOI https://doi.org/10.1007/s13213-012-0467-y
30 Savitha J, Srividya S, Jagat R, Payal P, Priyanki S, Rashmi GW, Roshini KT, Shantala YM. Identification of potential fungal strains for the production of inducible, extracellular and alkalophilic lipase. African Journal of Biotechnology. 2007; 6(5): 564-568
31 Golani M, Hajela K, Pandey, GP. Screening, Identification, Characterization and Production of Bacterial Lipase from Oil Spilled Soil. International Journal of Current Microbiology and Applied Sciences. 2016; 5(3): 745-763. http://dx.doi.org/10.20546/ijcmas.2016.503.08
32 Supakdamrongkul P, Bhumiratana A, Wiwat, C. Optimization of extracellular lipase production from the biocontrol fungus Nomuraea rileyi. Biocontrol Science Technology. 2010; 20: 595-604. https://doi.org/10.1080/09583151003661177.
33 Sopuruchukwu IF, Nididi EM, Okerentugba PO. Optimization of Process Parameters for the Production of Lipase in Submerged Batch Fermentation by Fusarium sp. Journal of Pharmaceutical Biological Science. 2015; 10:70-78. https://doi.org/10.9790/300810237078
34 Rani C, Panneerselvam A. Influence of environmental and nutritional parameters on lipase production. Journal of Agricultural and Biological Sciences. 2009; 4(5): 39-43.
35 Rajesh EM, Arthe R, Rajendran R, Balakumar C, Pradeepa N, Anitha S. Investigation of lipase production by Trichoderma reesei and optimization of production parameters. Electronic Journal of Environmental, Agri-cultural and Food Chemistry. 2010; 9(7): 1177-1189.
2 Jaeger KE, Eggert T. Lipases for biotechnology. Current Opinion in Biotechnology. 2002; 13(4): 390–397.
3 Goao XG, Cao SG, Zhang KC. Production, properties and application to nonaqueous enzymatic catalysis on lipase from a newly isolated Pseudomonas strain. Enzyme and Microbial Technology. 2000; 27(1-2): 74-82. https://doi.org/10.1016/S0141-0229(00)00191-5
4 Dalmou E, Montesinos JL, Lotti M, Casas C. Effect of different carbon sources on lipase production by Candida rugosa. Enzyme and Microbial Technology. 2000; 26 (9-10): 657-663. https://doi.org/10.1016/S0141-0229(00)00156-3
5 Cardenas F, Alvarez E, de Castro– Alvarez MS, Sanchez–Montero JM, Valmaseda M, Elson S, Sinisterra JV. Screening and catalytic activity in organic synthesis of novel fungal and yeast lipases. Journal of Molecular Catalysis B: Enzymatic. 2001; 14: 111-123. https://doi.org/10.1016/S1381-1177(00)00244-7
6 Thakur S. Lipases, its sources, properties and applications: A review, International Journal of Scientific and Engineering Research. 2012; 3(7): 1-29.
7 Haq IU, Ali S, Saleem A, Javed MM. Mutagenesis of Bacillus licheniformis through ethyl methyl sulphonate for alpha amylase production. Pakistan Journal of Botany. 2009; 41(3): 1489-1498.
8 Iftikhar T, Niaz M, Zia MA, Haq IU. Production of extracellular lipases by Rhizopus oligosporus in a stirred fermentor. Brazilian Journal of Microbiology. 2010; 41: 1124-1132. http://dx.doi.org/10.1590/S1517-83822010000400034
9 Iftikhar T, Niaz M, Abbas SQ, Zia MA, Ashraf I, Lee KJ, Haq IU. Mutation induced enhanced biosynthesis of lipases by Rhizopus oligosporus var. microsporus. Pakistan Journal of Botany. 2010; 42(2): 1235-1249.
10 Sarada S, Sreekanth B, Kant S, Banarjee R, Bhattacharya BC. Production and optimization of microbial lipase. Bioprocess Engineering. 1998; 19(1): 29-32.
11 Iftikar T, Haq IU, Javed MM. Optimization of culture conditions for the production of lipase by submerged culture of Rhizopus oligosporus Tuv-31, Pakistan Journal of Botany. 2003; 35: 519-525.
12 Matselis E, Roussis IG. Influence of culture conditions and hydrogen peroxide on growth and extracellular enzyme production by Pseudomonas UICD31. Food Science and Technology. 1992; 25(5): 433-437.
13 Makhzoum A, Knapp JS, Owusu RK. Factors affecting growth and extracellular lipase production by Pseudomonas fluorescens 2D. Food Microbiology. 1995; 12(4): 277-290. https://doi.org/10.1016/S0740-0020(95)80108-1
14 Ghosh PK, Saxena RK, Gupta R, Yadav RP, Davidson S. Microbial Lipases: Production and applications. Science Progress. 1996; 79(2): 119-157.
15 Elibol M, Ozer D. Influence of oxygen transfer on lipase production by Rhizopus arrhizus. Process Biochemistry. 2001; 36(4): 325-329. https://doi.org/10.1016/S0032-9592(00)00226-0
16 Shukla BN, Desai PV. Isolation, Characterization and Optimization of Lipase Producing Pseudomonas spp. from Oil Contaminated Sites. International Journal of Current Microbiology and Applied Sciences. 2016; 5(5): 902-909. http://dx.doi.org/10.20546/ijcmas.2016.505.093
17 Wadia T, Jain SK. Isolation, Screening and identification of Lipase Producing Fungi from Oil Contaminated Soil of Shani Mandir Ujjain. International Journal of Current Microbiology and Applied Sciences. 2017; 6(7): 1872-1878. https://doi.org/10.20546/ijcmas.2017.607.223
18 Colla LM, Primaz AL, Benedetti S, Loss RA, Lima MD, Reinehr CO, Bertolin TE, Costa JA. Selection of Lipase –Producing Microorganisms through Submerged Fermentation. Zeitschrift fur Naturforschung C. 2010; 65(7-8): 483-488. https://doi.org/10.1515/znc-2010-7-811.
19 Singh C, Parmar RS, Jadon P, Kumar A. Optimization of Cultural Conditions for Production of Antifungal Bioactive Metabolites by Streptomyces spp. Isolated from Soil. International Journal of Current Microbiology and Applied Sciences. 2017; 6(2): 386-396. http://dx.doi.org/10.20546/ijcmas.2017.602.043
20 Kapoor A, Sharma S, Prakash S. Optimization of Culture Conditions for the Production of Lipase from Gliomastix indicus and its Enzymatic Properties. Dynamic Biochemistry, Process Biotechnology and Molecular Biology. 2012; 6(1): 118-122.
21 Joshi GK, Kumar S, Tripathi BN, Sharma V. Production of alkaline lipase by Corynebacterium paurometabolum MTCC6841 isolated from Lake Naukuchiatal, Uttaranchal State, India. Current Microbiology. 2006; 52(5): 354-358. https://doi.org/10.1007/s00284-005-0224-6
22 Lowry OH, Rosenbrough NJ, Farr AL, Randall A. Protein measurement with the folin phenol reagent. Journal of Biological Chemistry. 1951; 193(1): 265-275.
23 Golani M, Hajela K, Pandey GP. Screening, Identification, Characterization and Production of Bacterial Lipase from Oil Spilled Soil. International Journal of Current Microbiology and Applied Sciences 2013; 5(3): 745-763. http://dx.doi.org/10.20546/ijcmas.2016.503.08
24 Valéria MG, Nadia K, Sarquis MIM, Mitchell DA, Luiz PR, Fontana JD. Effect of Nitrogen and Carbon Sources on Lipase Production by Penicillium aurantiogriseum. Food Technology Biotechnology. 2003; 41(2): 105–110.
25 Sneha PM, Parimala T, Rao CSVR, Satish BR. Effect of various sources and parameters on the production of an extracellular lipase from Aspergillus heteromorphus and optimization using response surface methodology. Journal of Chemical, Biological and Physical Sciences. 2012; 2(1): 212-222.
26 Maia MDMD, Morais MMCD, Morais MAD, Melo EHM, Filho JLDL. Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FSI, Revista de Microbiologia. 1999; 30: 304-309.
27 Fan Y, Shang L, Qian J. Screening, identifying and medium optimization of a lipase producing filamentous fungus from soil for high chiral resolution of 1- phenylethanol. African Journal of Microbiology Research. 2003; 7(33): 4235-4243.
28 Bokhari DN, Gohar UF, Hussain Z. Optimization Studies of Lipase Production from Locally isolated Bacillus sp. Biologia (Pakistan). 2013; 59(2): 259-265.
29 Sethi BK, Rout JR, Das R, Nanda PK, Sahoo SL. Lipase production by Aspergillus terreus using mustard seed oil cake as a carbon source. Annals of Microbiology. 2013; 63(1): 241-252. DOI https://doi.org/10.1007/s13213-012-0467-y
30 Savitha J, Srividya S, Jagat R, Payal P, Priyanki S, Rashmi GW, Roshini KT, Shantala YM. Identification of potential fungal strains for the production of inducible, extracellular and alkalophilic lipase. African Journal of Biotechnology. 2007; 6(5): 564-568
31 Golani M, Hajela K, Pandey, GP. Screening, Identification, Characterization and Production of Bacterial Lipase from Oil Spilled Soil. International Journal of Current Microbiology and Applied Sciences. 2016; 5(3): 745-763. http://dx.doi.org/10.20546/ijcmas.2016.503.08
32 Supakdamrongkul P, Bhumiratana A, Wiwat, C. Optimization of extracellular lipase production from the biocontrol fungus Nomuraea rileyi. Biocontrol Science Technology. 2010; 20: 595-604. https://doi.org/10.1080/09583151003661177.
33 Sopuruchukwu IF, Nididi EM, Okerentugba PO. Optimization of Process Parameters for the Production of Lipase in Submerged Batch Fermentation by Fusarium sp. Journal of Pharmaceutical Biological Science. 2015; 10:70-78. https://doi.org/10.9790/300810237078
34 Rani C, Panneerselvam A. Influence of environmental and nutritional parameters on lipase production. Journal of Agricultural and Biological Sciences. 2009; 4(5): 39-43.
35 Rajesh EM, Arthe R, Rajendran R, Balakumar C, Pradeepa N, Anitha S. Investigation of lipase production by Trichoderma reesei and optimization of production parameters. Electronic Journal of Environmental, Agri-cultural and Food Chemistry. 2010; 9(7): 1177-1189.
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08-09-2018
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Shreya ., Sharma AK, Sharma V, Saxena J. Optimization of culture conditions for extracellular fungal lipase production by submerged fermentation process. Plant Sci. Today [Internet]. 2018 Sep. 8 [cited 2024 Nov. 21];5(3):135-41. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/399
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