Antioxidant and antidiabetic activity of the endophytic fungus Alternaria alternata isolated from Rosmarinus officinalis L.
DOI:
https://doi.org/10.14719/pst.4003Keywords:
Alternaria alternata, antidiabetic, antioxidant, indoor fungi, rosemary plantAbstract
The results of the isolation of internal fungi showed that 70% of the leaves of the rosemary plant that were examined contained one isolation of the endophytic fungi by one fungal colony for each plant piece of rosemary plant leaves. The results of the phenotypic and microscopic examination of the internal fungus isolates showed that they belong to the same species: the fungus Alternaria alternata. The diagnosis was confirmed by PCR technique, and the isolates were compared with the global isolates registered in the gene bank (GenBank MF099865.1 and OQ764793.1), with which they matched 100%. The results of the evaluation of the biological efficacy of the extract of the internal fungi A. alternata showed that it possesses vital properties of an antioxidant by inhibiting the activity of free radicals 2,2-diphenyl-1-picrylhydrazyl (DPPH) The extract internal fungi recorded a high percentage of inhibitory activity of free radicals and was 93.5% at a concentration of 500 mg, we note that the inhibitory effect increased with increasing concentration of the extract. The results of the current study also showed that the extract of the internal fungi A. alternata. It showed efficiency in inhibiting the activity of the alpha-amylase enzyme, and therefore, it works as an antidiabetic, as it achieved an inhibition rate of alpha-amylase activity of 75.55%.
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Lata R, Chowdhury S, Gond SK, White Jr JF. Induction of abiotic stress tolerance in plants by endophytic microbes. Letters in applied microbiology. 2018; 66(4):268-76. https://doi.org/10.1111/lam.12855
Chhipa H, Deshmukh SK. Fungal endophytes: rising tools in sustainable agriculture production. Endophytes and secondary metabolites. Springer International Publishing AG, Cham. 2019:1-24. https://doi.org/10.1007/978-3-319-90484-9_26
Gouda S, Das G, Sen SK, Shin HS, Patra JK. Endophytes: a treasure house of bioactive compounds of medicinal importance. Frontiers in microbiology. 2016;7:219261. https://doi.org/10.3389/fmicb.2016.01538
Manganyi MC, Ateba CN. Untapped potentials of endophytic fungi: A review of novel bioactive compounds with biological applications. Microorganisms. 2020; 8(12):1934. https://doi.org/10.3390/microorganisms8121934
White JF, Kingsley KL, Zhang Q, Verma R, Obi N, Dvinskikh S, Elmore MT, Verma SK, Gond SK, Kowalski KP. Endophytic microbes and their potential applications in crop management. Pest management science. 2019; 75(10): 2558-2565. https://doi.org/10.1002/ps.5527
Ji HF, Li XJ, Zhang HY. Natural products and drug discovery: can thousands of years of ancient medical knowledge lead us to new and powerful drug combinations in the fight against cancer and dementia?. EMBO reports. 2009; 10(3):194-200. https://doi.org/10.1038/embor.2009.12
Kusari S, Hertweck C, Spiteller M. Chemical ecology of endophytic fungi: origins of secondary metabolites. Chemistry & Biology. 2012; 19(7):792-8. https://doi.org/10.1016/j.chembiol.2012.06.004
AL-Masoody MM, Stanica F. Effect of growth regulators for the induction of Callus from the apical bud on In Vitro of Rosemary Plant (Rosmarinus officinalis L.). International Journal of Engineering and Technology. 2015; 2(3):811-814.
Al-Sereiti MR, Abu-Amer KM, Sena P. Pharmacology of rosemary (Rosmarinus officinalis Linn.) and its therapeutic potentials. Indian J Exp Biol. 1999.37(2):30-124. https://pubmed.ncbi.nlm.nih.gov/10641130/
Aguilar F, Autrup H, Barlow S, Castle L, Crebelli R, Dekant W, Engel KH, Gontard N, Gott D, Grilli S, Gürtler R. Use of rosemary extracts as a food additive-Scientific opinion of the panel on food additives, flavourings, processing aids and materials in contact with food. EFSA J. 2008;721:1-29. https://doi.org/10.2903/j.efsa.2008.721
Araújo WL, Marcon J, Maccheroni Jr W, Van Elsas JD, Van Vuurde JW, Azevedo JL. Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plants. Applied and Environmental Microbiology. 2002; 68(10):4906-14. https://doi.org/10.1128/AEM.68.10.4906-4914.2002
Hospenthal DR, Beckius ML, Floyd KL, Horvath LL, Murray CK. Presumptive identification of Candida species other than C. albicans, C. krusei, and C. tropicalis with the chromogenic medium CHROMagar Candida. Annals of Clinical Microbiology and Antimicrobials. 2006 Jan;5:1-5. https://doi.org/10.1186/1476-0711-5-1
Watanabe T. Pictorial Atlas of Soil and Seed Fungi Morphologies of Cultured Fungi and Key to Species. 2nd. Edition, CRC Press,2002, 486. https://doi.org/10.1201/9781420040821
Ellis D., Davis S., Alexiou H, Handke R, Bartley R. Descriptions of Medical Fungi.2 ed. The National Library of Australia Cataloguing-in-Publication,2007.Pages 204.
Pryor BM. and Michailides TJ. Morphological, pathogenic, and molecular characterization of Alternaria isolates associated with Alternaria late blight of pistachio. Phytopathology. 2002. 92: 406- 416. https://doi.org/10.1094/PHYTO.2002.92.4.406
Simmons EG. Alternaria: An Identification Manual. CBS fungal Biodiversity centre. The Netherlands, 2007. https://www.nhbs.com/alternaria-an-identification-manual-book
Gannibal PB, Lawrence DP. Distribution of Alternaria species among sections. 3. Sections Infectoriae and Pseudoalternaria. Mycotaxon. 2016;131(4):781-90. https://doi.org/10.5248/131.781
Poursafar A, Ghosta Y, Orina AS, Gannibal PB, Javan-Nikkhah M, Lawrence DP. Taxonomic study on Alternaria sections Infectoriae and Pseudoalternaria associated with black (sooty) head mold of wheat and barley in Iran. Mycological Progress. 2018; 17:343-56. https://doi.org/10.1007/s11557-017-1358-1
Chambers SM, and Scott ES. In vitro antagonism of Phytophthora cinnamomi and P. citricola by isolates of Trichoderma spp. and Gliocladium virens. Journal of Phytopathology, 1995; 143(8):471-477. https://doi.org/10.1111/j.1439-0434.1995.tb04557.x
Bhardwaj A, Sharma D, Jadon N, Agrawal PK. Antimicrobial and phytochemical screening of endophytic fungi isolated from spikes of Pinus roxburghii. Archives of Clinical Microbiology, 2015; 6(3):19.
Szakiel A, Voutquenne-Nazabadioko L, Henry M. Isolation and biological activities of lyoniside from rhizomes and stems of Vaccinium myrtillus. Phytochemistry Letters, 2011;4(2):138-143. https://doi.org/10.1016/j.phytol.2011.02.002
Devarajan S, Venugopal S. Antioxidant and ?-amylase inhibition activities of phenolic compounds in the extracts of Indian honey. Chinese Journal of Natural Medicines. 2012 Jul 1;10(4):255-9. https://doi.org/10.1016/S1875-5364(12)60051-X
Abdulhadi SY, Hasan GQ, Gergees RN. Molecular detection and antimicrobial activity of endophytic fungi isolated from a medical plant Rosmarinus officinalis. arXiv preprint arXiv:2303.05242. 2023 Mar 9. https://arxiv.org/abs/2303.05242
Moharram AM, Zohri AA, Seddek NH. Production of kojic acid by endophytic fungi isolated from medicinal plant in Egypt. Int Invent J Biochem Bioinf. 2015;3(3):28-31.
Zaghir FS. Characterization of isolates of the Alternaria fungus that produces the toxin Alternariol in tomato quantities and a study of its advanced toxicological effects. Doctoral dissertation. College of Education for Pure Sciences, University of Karbala, Iraq, 2015.
Konstantinova P, Bonants PJM, Van Gent-Pelzer MPE, Van Der Zouwen P. and Van Den Bulk R. Development of specific primers for detection and identification of Alternaria spp. in carrot material by PCR and comparison with blotter and plating assays. Mycol. Res, 2002. 106: 23-33. https://doi.org/10.1017/S0953756201005160
Mmbaga MT, Shi A, Kim M. Identification of Alternaria alternata as a causal agent for Leaf Blight in Syringa Species, Plant Pathol J. 2011;27(2): 120-127. https://api.semanticscholar.org/CorpusID:55122316
Sandoval-Denis M, Sutton DA, Martin-Vicente A, Cano-Lira JF, Wiederhold N, Guarro J, Gené J. Cladosporium species recovered from clinical samples in the United States. Journal of Clinical Microbiology, 2015;53(9), 2990-3000. https://journals.asm.org/doi/10.1128/jcm.01482-15
Rezaei-Matehkolaei A, Makimura K, de Hoog S, Shidfar MR, Zaini F, Eshraghian M, Naghan PA and Mirhendi H. Molecular epidemiology of dermatophytosis in Tehran, Iran, a clinical and microbial survey. Medical Mycology, 2013;51(2):203-207. https://doi.org/10.3109/13693786.2012.686124
Kress WJ, Wurdack KJ, Zimmer EA, Weigt LA, and Janzen DH. Use of DNA barcodes to identify flowering plants. Proceedings of the National Academy of Sciences, 2005.102(23):8369-8374. https://doi.org/10.1073/pnas.0503123102
Bellemain E, Carlsen T, Brochmann C, Coissac E, Taberlet P, and Kauserud H. ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases. BMC Microbiology, 2010. 10(1): 1-9.https://doi.org/10.1186/1471-2180-10-189
Fadiji AE, and Babalola OO. Exploring the potentialities of beneficial endophytes for improved plant growth. Saudi Journal of Biological Sciences, 2020.27(12):3622. https://doi.org/10.1016/j.sjbs.2020.08.002
Gauchan DP, Kandel P, Tuladhar A, Acharya A, Kadel U, Baral A, Shahi AB, and García-Gil MR. Evaluation of antimicrobial, antioxidant, and cytotoxic properties of bioactive compounds produced from endophytic fungi of Himalayan yew
(Taxus wallichiana) in Nepal. F1000 Research, 2020. 9:379. https://doi.org/10.12688/f1000research.23250.2
Mehta JL, Rasouli N, Sinha AK, Molavi B. Oxidative stress in diabetes: a mechanistic overview of its effects on atherogenesis and myocardial dysfunction. International Journal of Biochemistry and Cell Biology, 2006. 38(5-6):794-803. https://doi.org/10.1016/j.biocel.2005.12.008
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