DNA barcode based cultivar authentication in Clitoria ternatea L. using rpoB and rbcL conserve regions
DOI:
https://doi.org/10.14719/pst.4403Keywords:
DNA barcode, Clitoria ternatea, rpoB, rbcL, SNPs, insertion, deletionAbstract
Clitoria ternatea (C. ternatea), generally known as Shankhpushpi, is an essential substance in Ayurvedic medicine used for memory enhancement, and as a nootropic, tranquillizing, anti-stress, anxiolytic, anti-convulsant and sedative agent. Identification of the vegetative state of cultivars is a challenging action within the species. In the current study, 6 cultivars were recognized by using universally conserved sequences. DNA barcoding is based on molecular markers technology, typically used for diversity or species identification. To investigate 6 distinct C. ternatea L. varieties that were collected in and around Bhubaneswar, Odisha, we employed 9 conserved locus barcode locations: atpF-atpH, rpoB, matK, rpoC, psbK-psbI, trnH-psbA and rbcL. After PCR, gel electrophoresis yielded significantly distinct bands for 5 markers: rbcL, rpoB, atpF-atpH, trnH-psbA and psbK-psbI. Phylogenetic analysis and sequencing of the amplified bands using the rpoB and rbcL barcode primers revealed insertion and deletion, which were suggestive of consistent separation of all 6 cultivars. Consequently, these markers may be utilized to identify the cultivars of C. ternatea. These barcode markers may also be used to recognize different C. ternatea cultivars.
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