Enhanced antagonistic potential of gamma-irradiated Trichoderma spp. against Phytophthora capsici : An in vitro study
DOI:
https://doi.org/10.14719/pst.4898Keywords:
antagonistic, gamma irradiation, Phytophthora capsici, Trichoderma spp.Abstract
Enhancing the biocontrol efficacy of microorganisms is a crucial element in advancing sustainable agricultural practices, particularly in the management of soilborne fungal phytopathogens. Among the various methods employed to augment the effectiveness of biocontrol agents, gamma radiation has emerged as a particularly promising approach due to its ability to induce genetic mutations that can enhance antagonistic properties. In the present study, an efficient gamma irradiation technique was employed to induce genetic modifications in three biocontrol agents: Trichoderma guizhouense , T. koningiopsis , and T. asperellum. The efficacy of these genetically altered strains was then evaluated against the pathogen Phytophthora capsici. Mutant isolates were generated through exposure to varying doses of gamma radiation and were initially screened based on their growth rates. These were subsequently subjected to a series of in vitro antagonistic tests. The results demonstrated that five selected mutants Ta 200-1, Ta 250-2, Tg 200-1, Tg 250-2, and Ta 150-3 exhibited significantly enhanced antagonistic potential compared to their parent strains. Importantly, these mutants retained their improved traits even after ten rounds of subculturing, with growth inhibition percentages ranging from 72.11% to 68.11%. The findings from the in vitro antagonistic studies suggest that gamma irradiation represents an effective strategy for enhancing the antagonistic capabilities of Trichoderma species, thereby contributing to the development of more robust biocontrol agents in plant health management.
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Misra AK, Prasad B. Trichoderma–a genus for biocontrol. Bi-opesticide and bioagents in integrated pest management of agriculture crops, RP Shrivastava (ed.) International Book Distri-bution Co., Lucknow. 2003:811-33.
Nakkeeran S, Vinodkumar S, Priyanka R, Renukadevi P. Mode of action of Trichoderma spp. in biological control of plant diseas-es. Biocontrol of Soil Borne Pathogens and Nematodes. 2018:81-95.
Mohamed HA, Haggag WM. Mutagenesis and inter-specific pro-toplast fusion between Trichoderma koningii and Trichoderma reesei for biocontrol improvement. American Journal of Scien-tific and Industrial Research. 2010;1(3):504-15.https://doi.org/10.5251/ajsir.2010.1.3.504.515
Najafi N, Hosseini R, Ahmadi A. Impact of gamma rays on the Phaffia rhodozyma genome revealed by RAPD-PCR. Iranian Journal of Microbiology. 2011;3(4):216.
Shahbazi S, Ispareh K, Karimi M, Askari H, Ebrahimi MA. Gamma and UV radiation induced mutagenesis in Trichoderma reesei to enhance cellulases enzyme activity. International Journal of Farming and Allied Sciences. 2014;5:543-54.
Bagheri K, Shahbazi S, Askari H, Mojerlou S, Amirlou F. Cellulase enzyme production enhancement in Trichoderma viride by gamma ray induced mutation. Nova Biologica Reperta. 2018;4(4):329-36.https://doi.org/10.29252/nbr.4.4.329
Ghasemi S, Safaie N, Shahbazi S, Shams-Bakhsh M, Askari H. Enhancement of lytic enzymes activity and antagonistic traits of Trichoderma harzianum using ?-radiation induced mutation. Journal of Agricultural Science and Technology. 2019;21(4):1035-48.
Soufi E, Safaie N, Shahbazi S, Mojerlou S. Gamma irradiation induces genetic variation and boosting antagonism in Tricho-derma aureoviride. Archives of Phytopathology and Plant Pro-tection.2021;54(19-20):1649-74. https://doi.org/10.1080/03235408.2021.1936377
Cyriac A. Strain improvement of Trichoderma spp. by protoplast fusion (Doctoral dissertation, Department of Plant Patholgy, College of Agriculture, Vellayani).
Nair A, Sible GV, Cyriac A, Thara SS, Johnson JM, Radhika NS, Soni KB. Characterization of novel strains of Trichoderma spp. and their utilization in management of damping off disease in tomato. Journal of Biological Control. 2022;36(1):31–46. https://doi.org/10.18311/jbc/2022/30015
Jeevidha M, Sible GV, Cyriac A, Nair A, Radhakrishnan NV, John-son JM, Anuradha SS. Assessment of the biocontrol attributes of native Trichoderma isolates and their field evaluation against Fusarium wilt of vegetable cowpea. The Pharma Innovation Journal. 2023;12(8): 32-40.
Cullings K. Design and testing of a plant specific PCR primer for ecological and evolutionary studies. Molecular ecology. 1992;1(4):233-40.https://doi.org/10.1111/j.1365-294x.1992.tb00182.x
White TJ. Amplification and direct sequencing of fungal riboso-mal RNA genes for phylogenetics. PCR Protocols: A guide to methods and applications/Academic Press, Inc. 1990.https://doi.org/10.1016/b978-0-12-372180-8.50042-1
Dennis C, Webster J. Antagonistic properties of species-groups of Trichoderma. II. Production of volatile antibiotics. Transac-tions of the British Mycological Society. 1971;57: 363-69.
Gopinath PP, Parsad R, Joseph B, Adarsh VS. GrapesAgri1: col-lection of shiny apps for data analysis in agriculture. Journal of Open Source Software. 2021;6(63):3437. https://doi.org/10.21105/joss.03437
Druzhinina I, Kubicek CP. Species concepts and biodiversity in Trichoderma and Hypocrea: from aggregate species to species clusters. Journal of Zhejiang University-Science B. 2005;6(2):100-12. https://doi.org/10.1631/jzus.2005.b0100
Ch Persoon. Disposita methodical fungorum. Romers Neues Mag Bot. 1794;1:81-128.
Druzhinina IS, Kopchinskiy AG, Kubicek CP. The first 100 Tricho-derma species characterized by molecular data. Mycoscience. 2006;47(2):55-64. https://doi.org/10.1007/s10267-006-0279-7
Dou K, Lu Z, Wu Q, Ni M, Yu C, Wang M, Li Y, Wang X, Xie H, Chen J, Zhang C. MIST: A multilocus identification system for Tricho-derma. Applied and Environmental Microbiology. 2020;86(18):e01532-20.https://doi.org/10.1007/s10267-006-0279-7
Spadaro D, Gullino ML. Improving the efficacy of biocontrol agents against soilborne pathogens. Crop protection. 2005;24(7):601-13.https://doi.org/10.1016/j.cropro.2004.11.003
Steensels J, Snoek T, Meersman E, Nicolino MP, Voordeckers K, Verstrepen KJ. Improving industrial yeast strains: exploiting natural and artificial diversity. FEMS Microbiology Reviews. 2014;38(5):947-95.https://doi.org/10.1111/1574-6976.12073
Barkai-Golan R. Suppression of postharvest pathogens of fresh fruits and vegetables by ionizing radiation. Electromagnetic Radiation in Food Science, Springer-Verlag, Berlin, Heidelberg. 1992:155-93.
Tauxe RV. Food safety and irradiation: protecting the public from foodborne infections. Emerging infectious diseases. 2001;7(3 Suppl):516.https://doi.org/10.3201/eid0707.017706
Diep TB, Thom NT, Sang HD, An TX, Van Binh N, Quynh TM. Effect of gamma irradiation on the viability and cellulase pro-duction of some filamentous fungi. Vietnam Journal of Biotech-nology. 2020;18(2):341-8.https://doi.org/10.15625/1811-4989/18/2/15280
Baharvand A, Shahbazi S, Afsharmanesh H, Ali M. Investigation of gamma irradiation on morphological characteristics and antagonist potential of Trichoderma viride against M. phaseolina. International Journal of Farming and Allied Scienc-es. 2014;3(11):1157-64.https://doi.org/10.15835/nsb447818
Elkenawy NM, Yassin AS, Elhifnawy HN, Amin MA. Optimization of prodigiosin production by Serratia marcescens using crude glycerol and enhancing production using gamma radiation. Biotechnology reports. 2017;14:47-53.https://doi.org/10.1016/j.btre.2017.04.001
Abbasi S, Safaie N, Shams-bakhsh M. Evaluation of gamma-induced mutants of Trichoderma harzianum for biological con-trol of charcoal rot of melon (Macrophomina phaseolina) in laboratory and greenhouse conditions. Journal of Crop Protec-tion. 2014;3(4):509-21.
Wagh S, Ingle ST, Dandale S, Mane SS. Improvement in biocon-trol ability of Trichoderma viride through gamma irradiation. Trends in Biosciences. 2015;8(20):5622-6.
Shahbazi S, Karimi M, Ebrahimi MA. Investigation of sequence-tagged site (STS) in chitinase gene of gamma radiated Tricho-derma viride isolates. International Journal of Farming and Allied Sciences. 2014;3(11):1129-36.
Askari H, Soleimanian-Zad S, Kadivar M, Shahbazi S. Creating a novel genetic diversity of Trichoderma afroharzianum by ?-radiation for xylanase-cellulase production. Heliyon. 2024;10(7).https://doi.org/10.1016/j.heliyon.2024.e28349
Darabzadeh N, Hamidi-Esfahani Z, Hejazi P. Improvement of cellulase production and its characteristics by inducing muta-tion on Trichoderma reesei 2414 under solid state fermentation on rice by-products. Applied Food Biotechnology. 2018;5(1):11-8.
Cai F, Kubicek CP, Druzhinina IS. Genetic transformation of Trichoderma spp. InBiofuels and Biodiesel 2021;pp. 171-185. New York, NY: Springer US.https://doi.org/10.1007/978-1-0716-1323-8_12
Howell CR. Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolu-tion of current concepts. Plant disease. 2003;87(1):4-10.https://doi.org/10.1094/pdis.2003.87.1.4
Singh M, Chauhan A, Singh PK. Enhanced growth and suppres-sion of Fusarium wilt in tomato plants through the synergistic action of Rhizophagus intraradices and Trichoderma viride. Vegetos, https://doi.org/10.1007/s42535-024-00935-y
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