Unveils the metabolic changes in groundnut CO 7 kernels stored under modified atmospheric conditions
DOI:
https://doi.org/10.14719/pst.4997Keywords:
Groundnut, GC-MS, metabolic profiling, enzyme activity, seed quality, modified atmospheric storageAbstract
Storing groundnut kernels is more challenging than storing pods due to increased oxidative damage. Pod storage requires more space and is not cost-effective for long-term storage. Therefore, an attempt was made to store kernels under modified conditions for six months to address these storage issues. An experiment was performed to evaluate the effects of different modified atmospheric storage conditions on the groundnut cultivar CO 7, in both shelled and unshelled forms. Changes in seed quality parameters and metabolic profiles were analyzed during storage at intervals of 30 days and up to 180 days. The kernels stored under nitrogen and vacuum conditions maintained their seed quality traits, including physical, physiological, and biochemical characteristics. GC-MS analysis was performed at the beginning of storage (fresh seeds) and after 180 days (aged seeds). Sixteen metabolic compounds were identified as responsible for seed viability and deterioration, with sucrose being the predominant compound. The sucrose area percentage in fresh seeds was 52.52%, which decreased during aging. At the end of the storage period, the highest sucrose area percentages were found in kernels stored under nitrogen (28.9%) and vacuum (26.95%) conditions. This study concludes that groundnut kernels stored under nitrogen conditions have good storage potential and excel at maintaining seed longevity.
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Variath MT, Janila P. Economic and academic importance of peanut. In: Varshney R, Pandey M, Puppala N (eds) The Peanut Genome. Compendium of Plant Genomes. Springer, Cham. 2017. https://doi.org/10.1007/978-3-319-63935-2_2
Lah NHC, El Enshasy HA, Mediani A, Azizan KA, Aizat WM, Tan JK, Afzan A, Noor NM, Rohani ER. An insight into the behaviour of recalcitrant seeds by understanding their molecular changes upon desiccation and low temperature. Agronomy. 2023;13(8):2099. https://doi.org/10.3390/agronomy13082099
Vasudevan S, Shakuntala N, Teli S, Goud S, Gowda B. Studies on effect of modified atmospheric storage condition on storability of groundnut (Arachis hypogaea L.) seed kernels. Int J Res Stud Biosci. 2014;2(2):25-36.
Jyoti J, Malik C. Seed deterioration: a review. Int J LifeSc Bt Pharm Res. 2013; 2(3):374-385.
Mahjabin SB, Abidi A. Physiological and biochemical changes during seed deterioration: a review. Int J Recent Sci Res. 2015;6(4):3416-22.
Sujatha M, Uppar D, Biradaeatil N, Mummigatti U, Nagaratna S. Olekar. Biochemical changes in storage of groundnut seeds under different locations, storage containers and storage conditions. Int J Curr Microbiol Appl Sci. 2018;7:297-305. https://doi.org/10.20546/ijcmas.2018.707.036
Ziegler V, Paraginski RT, Ferreira CD. Grain storage systems and effects of moisture, temperature and time on grain quality-A review. J Stored Prod Res. 2021;91:101770. https://doi.org/10.1016/j.jspr.2021.101770
Gayathri M, Jerlin R, Eevera T, Amuthaselvi G. Storage behaviour of groundnut (Arachis hypogaea L.) seeds under different storage condition. Agric Sci Dig. 2024;4(1):156-163. https://doi.org/10.18805/ag.D-5793
Punithavathi R, Parameswari K, Umarani R, Eevera T, Anand T, Gomathi V. Exploring the storage potential of groundnut Var. TMV (Gn) 13 under modified atmospheric storage conditions. Int J Environ Clim Chang. 2023;13(10):3982-89. https://doi.org/10.9734/ijecc/2023/v13i103072
Gunathilake CC. Effect of modified atmospheric conditions to soybean seeds under hermetically sealed large capacity storage. Agric Eng Int: CIGR Journal. 2020;22(3):213-18.
Capilheira AF, Cavalcante JA, Gadotti GI, Bezerra BR, Hornke NF, Villela FA. Storage of soybean seeds: Packaging and modified atmosphere technology. Rev Bras Eng Agríc. Ambient. 2019;23(11):876-82. https://doi.org/10.1590/1807-1929/agriambi.v23n11p876-882
Bailly C. Active oxygen species and antioxidants in seed biology. Seed Sci Res. 2004;14(2):93-107. https://doi.org/10.1079/SSR2004159
Divya P, Durga KK, Sunil N, Rajasri M, Keshavulu K, Udayababu P. Modified atmosphere storage technique for the management of pulse beetle, Callosobruchus chinensis in Horse gram. Legume Res. 2016;39(3):474-78. https://doi.org/10.18805/lr.v0iOF.9610
ISTA. International rules for seed testing. 2019;356-513. https://www.seedtest.org/en/international-rules-for-seed-testing-rubric-3.html
Gupta PC. Seed vigour testing. Handbook of Seed Testing. 1993;242-249.
Abdul?Baki AA, Anderson JD. Vigor determination in soybean seed by multiple criteria 1. Crop Sci. 1973;13(6):630-33. https://doi.org/10.2135/cropsci1973.0011183X001300060013x
Presley JT. Relation of protoplast permeability to cotton seed viability and predisposition to seedling disease. Plant Dis Rep .1958;42(7):852
Kittock D, Law A. Relationship of seedling vigor to respiration and tetrazolium chloride reduction by germinating wheat seeds 1. Agron J. 1968;60(3):286-88. https://doi.org/10.2134/agronj1968.00021962006000030012x
Aebi H. Catalase in vitro. Methods Enzymol. 1984;105:121-26. https://doi.org/10.1016/S0076-6879(84)05016-3
Malik R, Singh C. The effect of organic acids and cycocel on peroxidase activity of cotton seedlings. Agrochimica. 1980;24(5/6):478-81.
Wen Z, Du X, Meng N, Li Y, Mi R, Li X, Sun Y, Ma S, Li S. Tussah silkmoth pupae improve anti-tumor properties of Cordyceps militaris (L.) Link by increasing the levels of major metabolite cordycepin. RSC Adv. 2019;9(10):5480-91. https://doi.org/10.1039/C8RA09491H
Doijode S. Seed germplasm conservation with modified atmosphere storage in Amaranth (Amaranthus spp.). Indian Journal of Plant Genetic Resources. 2001;14(2):288-89.
Tahir A, Afzal I, Khalid E, Razzaq M, Arif MAR. Rice seed longevity in the context of seed moisture contents and hypoxic conditions in the storage environment. Seed Sci. Res. 2023;33(1):39-49. https://doi.org/10.1017/S0960258522000289
Bass L. Seed viability during long-term storage. Hortic Rev.1982;2:117-141.
Yan S, Huang W, Gao J, Fu H, Liu J. Comparative metabolomic analysis of seed metabolites associated with seed storability in rice (Oryza sativa L.) during natural aging. Plant Physiol Biochem. 2018;127:590-98. https://doi.org/10.1016/j.plaphy.2018.04.020
Chen BX, Fu H, Gao JD, Zhang YX, Huang WJ, Chen ZJ, Yan SJ, Liu J. Identification of metabolomic biomarkers of seed vigor and aging in hybrid rice. Rice. 2022;15(1):7. https://doi.org/10.1186/s12284-022-00552-w
Sun WQ, Leopold AC. The Maillard reaction and oxidative stress during aging of soybean seeds. Physiol Plant. 1995;94(1):94-104. https://doi.org/10.1034/j.1399-3054.1995.940114.x
Narayana Murthy U, Sun WQ. Protein modification by Amadori and Maillard reactions during seed storage: roles of sugar hydrolysis and lipid peroxidation. J Exp Bot. 2000;51(348):1221-28. https://doi.org/10.1093/jexbot/51.348.1221
Voll LM, Abbasi AR. Are there specific in vivo roles for ?-and ?-tocopherol in plants? Plant Signal Behav. 2007;2(6):486-88. https://doi.org/10.4161/psb.2.6.4499
Corbineau F. The effects of storage conditions on seed deterioration and ageing: How to improve seed longevity. Seeds. 2024;3(1):56-75. https://doi.org/10.3390/seeds3010005
Y?ld?r?m M. Effects of coating treatment with aloe vera and glycerol on germination delay and seed viability of wheat under different water levels. J Agric Nat. 2020;23(4):994-1004. https://doi.org/10.18016/ksutarimdoga.vi.688270
Zhou W, Branch WD, Gilliam L, Marshall JA. Phytosterol composition of Arachis hypogaea seeds from different maturity classes. Molecules. 2018;24(1):106. https://doi.org/10.3390/molecules24010106
Aboobucker SI, Suza WP. Why do plants convert sitosterol to stigmasterol? Front Plant Sci. 2019;10:354. https://doi.org/10.3389/fpls.2019.00354
Jumina, Nurmala A, Fitria A, Pranowo D, Sholikhah EN, Kurniawan YS, Kuswandi B. Monomyristin and monopalmitin derivatives: Synthesis and evaluation as potential antibacterial and antifungal agents. Molecules. 2018;23(12):3141. https://doi.org/10.3390/molecules23123141
Vidigal D, Willems L, van Arkel J, Dekkers BJ, Hilhorst HW, Bentsink L. Galactinol as marker for seed longevity. Plant Sci. 2016;246:112-18. https://doi.org/10.1016/j.plantsci.2016.02.015
Lim GH, Singhal R, Kachroo A, Kachroo P. Fatty acid and lipid-mediated signaling in plant defense. Annu Rev Phytopathol. 2017;55(1):505-36. https://doi.org/10.1146/annurev-phyto-080516-035406
Wallis JG, Bengtsson JD, Browse J. Molecular approaches reduce saturates and eliminate trans fats in food oils. Front Plant Sci. 2022;13:908608. https://doi.org/10.3389/fpls.2022.908608
Chen BX, Peng YX, Yang XQ, Liu J. Delayed germination of Brassica parachinensis seeds by coumarin involves decreased GA4 production and a consequent reduction of ROS accumulation. Seed Sci Res. 2021;31(3):224-35. https://doi.org/10.1017/S0960258521000167
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