Nickel induced exposure analysis for toxic changes in growth and antioxidative enzymes in sesban eliciting biochemical sensitivity

S Mania; M Mohanty

doi:10.14719/pst.5084

Authors

S Mania Department of Biotechnology, Laboratory of Plant and Environmental Engineering, Rama Devi Women’s University, Bhubaneswar – 751 022, India https://orcid.org/0000-0001-7893-7176
M Mohanty Department of Biotechnology, Laboratory of Plant and Environmental Engineering, Rama Devi Women’s University, Bhubaneswar – 751 022, India https://orcid.org/0000-0002-2183-1888

DOI:

https://doi.org/10.14719/pst.5084

Keywords:

carotenoid, catalase, chlorophyll, germination index, nickel phytotoxicity, peroxidase

Abstract

Nickel (Ni) exposure in plants leads to severe toxicity problems, with effects varying depending on its exposure concentration. The present pot culture investigation assesses the phytotoxic effects of different Nickel (Ni) concentrations on various biochemical parameters of Sesbania. The study involves applying Nickel at 50, 100, 200, and 300 ppm along with a control group at 0 ppm for 30 days. Results revealed retarded growth, reduced pigment content, and enhanced antioxidative enzyme activity as nickel concentration increased. Exposure to Ni (100 ppm) and above severely affects seed germination, plant growth, and biomass production. Furthermore, relative phytotoxicity was evident from a 15% reduced germination rate and a fall in germination index from 10 to 8.5. The seedling vigour index was drastically reduced from 960 (control) to 93.5(300 ppm Ni). In addition to these, plant growth retardation was striking with root length stunted by 70% and shoot length by 50% in response to Ni (300 ppm). Chlorophyll and carotenoid contents decreased by nearly 50% with the 300-ppm nickel treatment. Although protein levels and antioxidant enzyme activity (catalase and peroxidase) showed a stimulatory response to 100 and 200 ppm Ni treatments, both suffered a sharp decline due to toxic stress at 300 ppm Ni. This study explicitly highlights the harmful effects of high doses of Ni, highlighting the sensitivity of various morphometric and biochemical parameters to Ni toxicity. These findings highlights the need to mitigate environmental contamination and adopt measures to protect plant health.

Downloads

Download data is not yet available.

References

Ningombam L, Hazarika BN, Yumkhaibam T, Heisnam P, Singh YD. Heavy metal priming plant stress tolerance deciphering through physiological, biochemical, molecular and omics mechanism. S Afr J Bot. 2024;168:16–25. https://doi.org/10.1016/j.sajb.2024.02.032

Panchal A, Maitreya B. A review on exploring the significance of micronutrients in crop production. Inter Assoc of Biol and Comput Digest. 2023;2(2):51–58. https://doi.org/10.56588/iabcd.v2i2.183

Vácha R. Heavy metal pollution and its effects on agriculture. Agron. 2021;11(9):1719. https://doi.org/10.3390/agronomy11091719

Jorjani S, Karaka? PF. Physiological and biochemical responses to heavy metals stress in plants. Inter J of Sec Metabolite. 2024;11(1):169–90. https://doi.org/10.21448/ijsm.1323494

Kumar V, Kumar P, Singh J. Contaminants in agriculture and environment: Health risks and remediation. Contaminants in Agriculture and Environment: Health Risks and Remediation. 2019;1–301. https://doi.org/10.26832/aesa-2019-cae

Gerendas J, Sattelmacher B. Influence of Ni supply on growth and nitrogen metabolism of Brassica napus L. grown with NH4NO3 or urea as N source. Ann Bot. 1999;83(1):65–71. https://doi.org/10.1006/anbo.1998.0789

Eskew DL, Welch RM, Cary EE. A simple plant nutrient solution purification method for effective removal of trace metals using controlled pore glass-8-hydroxyquinoline chelation column chromatography. Plant Physiol. 1984;76(1):103–05. https://doi.org/10.1104/pp.76.1.103

Eskew DL, Welch RM, Norvell WA. Nickel in higher plants. Plant Physiol. 1984;76(3):691–93. https://doi.org/10.1104/pp.76.3.691

Brown PH, Welch RM, Cary EE. Nickel: A micronutrient essential for higher plants. Plant Physiol. 1987;85(3):801–03. https://doi.org/10.1104/pp.85.3.801

Brown P, Welch R, Cary E, Checkai R. Micronutrients. J Plant Nutr. 1987;10(9):2125–35. https://doi.org/10.1080/01904168709363763

Brown PH, Welch RM, Madison JT. Effect of nickel deficiency on soluble anion, amino acid and nitrogen levels in barley. Plant Soil. 1990;125(1):19–27. https://doi.org/10.1007/BF00010740

Sinha S, Gupta AK. Translocation of metals from fly ash amended soil in the plant of Sesbania cannabina L. Ritz: Effect on antioxidants. Chemosphere. 2005;61(8):1204–14. https://doi.org/10.1016/j.chemosphere.2005.02.063

Dong X, Li Z, Wang Q, Xie Z, Li Y, Luo Y. Enhancing the growth performance of Sesbania cannabina using Ensifer alkalisoli and biochar under salt stress. Rhizosphere. 2024;100888. https://doi.org/10.1016/j.rhisph.2024.100888

Rodriguez N, Carusso S, Juárez Á, El Kassisse Y, Rodriguez Salemi V, de Cabo L. Effect of stabilization time and soil chromium concentration on Sesbania virgata growth and metal tolerance. J Environ Manage. 2023;345:118701. https://doi.org/10.1016/j.jenvman.2023.118701

Arnon DI. Copper enzymes in isolated chloroplasts polyphenoloxidase in Beta vulgaris. Plant Physiol. 1949;24(1):1–15. https://doi.org/10.1104/pp.24.1.1

Lichtenthaler HK. [34] Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. In: Methods in enzymology. Academic Press; 1987. 148:350–82. https://doi.org/10.1016/0076-6879(87)48036-1

Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72(1–2):248–54. https://doi.org/10.1016/0003-2697(76)90527-3

Chance B, Maehly AC. [136] Assay of catalases and peroxidases. Methods Biochem Anal. 1955;764–75. https://doi.org/10.1002/9780470110171.ch14

Mohanty M. Hexavalent chromium induced toxicological, physiological and biochemical alterations in Sesbania sesban L. seedlings. J Plant Physiol Pathol. 2014;02(03). https://doi.org/10.4172/2329-955x.1000129

Helaoui S, Mkhinini M, Boughattas I, Bousserrhine N, Banni M. Nickel toxicity and tolerance in plants. heavy metal toxicity and tolerance in Plants. A Biological, Omics and Genetic Eng App. 2023;231–50. https://doi.org/10.1002/9781119906506.ch11

Kumar S, Wang M, Liu Y, Fahad S, Qayyum A, Jadoon SA, et al. Nickel toxicity alters growth patterns and induces oxidative stress response in sweet potato. Front Plant Sci. 2022;13: 1054924. https://doi.org/10.3389/fpls.2022.1054924

Satpathy MR, Samantaray S. Assessment of impact of nickel stress on the accumulation, pigments and protein content of water hyacinth (Pontederia crassipes L.). South Asian J of Agri Sci. 2023;3(1):145–48. https://doi.org/10.22271/27889289.2023.v3.i1b.82

Tipu MI, Ashraf MY, Sarwar N, Akhtar M, Shaheen MR, Ali S, et al. Growth and physiology of maize (zea mays L.) in a nickel-contaminated soil and phytoremediation efficiency using EDTA. J Plant Growth Regul. 2020;40(2):774–86. https://doi.org/10.1007/s00344-020-10132-1

Mujeeb A, Iqbal MZ, Shafiq M, Kabir M, Farooqi Z ur R. Effects of nickel toxicity on seedling growth, photosynthetic pigments, carotenoids and phenols contents of cowpea Vigna unguiculata (L.). I J Environ Agric Biotechnol. 2019;4(2):341–48. https://doi.org/10.22161/ijeab/4.2.12

Do?ru A, Altunda? H, Dündar M?. The e?ect of nickel phytotoxicity on photosystem II activity and antioxidant enzymes in barley. Acta Biologica Szegediensis. 2021;65(1):1–9. https://doi.org/10.14232/abs.2021.1.1-9

Meers E, Van Slycken S, Adriaensen K, Ruttens A, Vangronsveld J, Du Laing G, et al. The use of bio-energy crops (Zea mays) for ‘phytoattenuation’ of heavy metals on moderately contaminated soils: A field experiment. Chemosphere. 2010;78(1):35–41. https://doi.org/10.1016/j.chemosphere.2009.08.015

Gashi B, Buqaj L, Vataj R, Tuna M. Chlorophyll biosynthesis suppression, oxidative level and cell cycle arrest caused by Ni, Cr and Pb stress in maize exposed to treated soil from the Ferronikel smelter in Drenas, Kosovo. Plant Stress. 2024;11:100379. https://doi.org/10.1016/j.stress.2024.100379

Van Assche F, Clijsters H. Effects of metals on enzyme activity in plants. Plant Cell Environ. 1990;13(3):195–206. https://doi.org/10.1111/j.1365-3040.1990.tb01304.x

Gajewska E, Wielanek M, Bergier K, Sk?odowska M. Nickel-induced depression of nitrogen assimilation in wheat roots. Acta Physiol Plant. 2009;31(6):1291–300. https://doi.org/10.1007/s11738-009-0370-8

Zhang S, Zhang H, Qin R, Jiang W, Liu D. Cadmium induction of lipid peroxidation and effects on root tip cells and antioxidant enzyme activities in Vicia faba L. Ecotoxicology. 2009; 18(7):814–23. https://doi.org/10.1007/s10646-009-0324-3

Ghosh S, Batra D, Kumar Y, Matta NK. Effects of heavy metals on seed protein fractions in chickpea, Cicer arietinum (L.). J of Appl and Nat Sci. 2022;14(1):225–32. https://doi.org/10.31018/jans.v14i1.3332

Xu X, Liu C, Zhao X, Li R, Deng W. Involvement of an antioxidant defence system in the adaptive response to cadmium in maize seedlings (Zea mays L.). Bull Environ Contam Toxicol. 2014;93(5):618–24. https://doi.org/10.1007/s00128-014-1361-z