Skip to main navigation menu Skip to main content Skip to site footer
Plant Science Today (PST)

Research Articles

Vol. 11 No. sp4 (2024): Recent Advances in Agriculture by Young Minds - I

Phylogenetic analysis of rice cultivars for CYP93G1 and functional evaluation for apigenin and biofilm formation

DOI
https://doi.org/10.14719/pst.5554
Submitted
5 October 2024
Published
15-11-2024

Abstract

Improving biological nitrogen fixation (BNF) in rice is a long-standing vision for the researchers as it decreases the reliance on surplus usage of nitrogenous fertilizers. Enhancing plant signaling molecules, such as flavonoids, facilitates improved interactions between plants and microbes, thereby promoting increased biofilm formation. Apigenin, a key flavonoid in root exudates, induces the expression of the gumD gene in microbes, which is essential for exopolysaccharide synthesis (EPS), promoting microbial colonization of roots, biofilm formation, and potentially enhancing BNF. A phylogenetic analysis of 280 rice lines from the 3K RG panel, targeting the candidate gene CYP93G1, which is involved in apigenin synthesis, enabled the selection of nine genotypes for evaluating apigenin levels and biofilm formation. The results revealed that, despite having lower levels of apigenin, the promising genotypes Gokulganja, Local Bhat, and Nona Bokra exhibited significant biofilm formation compared to the other genotypes, suggesting their potential as effective cultivars for genome editing to enhance BNF. This is the first study to assess these traits concerning a specific gene in a 3K panel. Our findings demonstrated that targeted manipulation of tricin flavone biosynthetic pathway genes in these selected genotypes could significantly boost BNF, promoting ecological rice cultivation practices and advancing environmental sustainability.

References

  1. Vinci G, Ruggieri R, Ruggeri M, Prencipe SA. Rice production chain: environmental and social impact assessment—a review. Agriculture. 2023;13(2):340. https://doi.org/10.3390/agriculture13020340
  2. Prasad R, Shivay YS, Kumar D. Current status, challenges and opportunities in rice production. Rice Production Worldwide. 2017;1-32.https://doi.org/10.1007/978-3-319-47516-5_1
  3. Martínez-Dalmau J, Berbel J, Ordóñez-Fernández R. Nitrogen fertilization. A review of the risks associated with the inefficiency of its use and policy responses. Sustainability. 2021;13(10):5625.https://doi.org/10.3390/su13105625
  4. Zhang J, Tong T, Potcho PM, Huang S, Ma L, Tang X. Nitrogen effects on yield, quality and physiological characteristics of giant rice. Agronomy. 2020;10(11):1816. https://doi.org/10.3390/agronomy10111816
  5. Soumare A, Diedhiou AG, Thuita M, Hafidi M, Ouhdouch Y, Gopalakrishnan S, et al. Exploiting biological nitrogen fixation: a route towards a sustainable agriculture. Plants. 2020;9(8):1011. https://doi.org/10.3390/plants9081011
  6. Yan D, Tajima H, Cline LC, Fong RY, Ottaviani JI, Shapiro HY, et al. Genetic modification of flavone biosynthesis in rice enhances biofilm formation of soil diazotrophic bacteria and biological nitrogen fixation. Plant Biotechnol J. 2022;20(11):2135-48. https://doi.org/10.1111/pbi.13902
  7. Wang D, Xu A, Elmerich C, Ma LZ. Biofilm formation enables free-living nitrogen-fixing rhizobacteria to fix nitrogen under aerobic conditions. The ISME Journal. 2017;11(7):1602-13. https://doi.org/10.1038/ismej.2017.27
  8. Jyoti K, Soni K, Chandra R. Optimization of the production of Exopolysaccharide (EPS) from biofilm-forming bacterial consortium using different parameters. The Microbe. 2024;4:100117. https://doi.org/10.1016/j.microbe.2023.100117
  9. Ajijah N, Fiodor A, Pandey AK, Rana A, Pranaw K. Plant growth-promoting bacteria (PGPB) with biofilm-forming ability: a multifaceted agent for sustainable agriculture. Diversity. 2023;15(1):112.https://doi.org/10.3390/d15010112
  10. Meneses CH, Rouws LF, Simões-Araújo JL, Vidal MS, Baldani JI. Exopolysaccharide production is required for biofilm formation and plant colonization by the nitrogen-fixing endophyte Gluconacetobacter diazotrophicus. Mol Plant Microbe Interact. 2011;24(12):1448-58. https://doi.org/10.1094/MPMI-07-11-0181
  11. Lam PY, Zhu F-Y, Chan WL, Liu H, Lo C. Cytochrome P450 93G1 is a flavone synthase II that channels flavanones to the biosynthesis of tricin O-linked conjugates in rice. Plant Physiol. 2014;165(3):1315-27.https://doi.org/10.1104/pp.114.239004
  12. Mannu J, Latha AM, Rajagopal S, Lalitha HDA, Muthurajan R, Loganathan A, et al. Whole genome sequencing of ASD 16 and ADT 43 to identify predominant grain size and starch associated alleles in rice. Mol Biol Rep. 2022;49(12):11743-54.https://doi.org/10.1007/s11033-022-07906-8
  13. Ayyenar B, Kambale R, Duraialagaraja S, Manickam S, Mohanavel V, Shanmugavel P, et al. Developing early morning flowering version of rice variety CO 51 to mitigate the heat-induced yield loss. Agriculture. 2023;13(3):553.https://doi.org/10.3390/agriculture13030553
  14. Valarmathi R, Raveendran M, Robin S, Senthil N. Unraveling the nutritional and therapeutic properties of ‘Kavuni’a traditional rice variety of Tamil Nadu. J Plant Biochem Biotechnol. 2015;24:305-https://doi.org/10.1007/s13562-014-0294-5 15.
  15. Yoshida S, Forno DA, Cock JH. Laboratory manual for physiological studies of rice. 1971.
  16. Williams A, Langridge H, Straathof AL, Fox G, Muhammadali H, Hollywood KA, et al. Comparing root exudate collection techniques: An improved hybrid method. Soil Biol Biochem. 2021;161:108391. https://doi.org/10.1016/j.soilbio.2021.108391
  17. Righini S, Rodriguez EJ, Berosich C, Grotewold E, Casati P, Falcone Ferreyra ML. Apigenin produced by maize flavone synthase I and II protects plants against UV?B?induced damage. Plant CelL Environ. 2019;42(2):495-508. https://doi.org/10.1111/pce.13437
  18. Guzmán-Soto I, McTiernan C, Gonzalez-Gomez M, Ross A, Gupta K, Suuronen EJ, et al. Mimicking biofilm formation and development: Recent progress in in vitro and in vivo biofilm models. Iscience. 2021;24(5). https://doi.org/10.1016/j.isci.2021.102443
  19. Lam PY, Liu H, Lo C. Completion of tricin biosynthesis pathway in rice: cytochrome P450 75B4 is a unique chrysoeriol 5?-hydroxylase. Plant Physiol. 2015;168(4):1527-36. https://doi.org/10.1104/pp.15.00622
  20. Badri DV, Vivanco JM. Regulation and function of root exudates. Plant Cell Environ. 2009;32(6):666-81. https://doi.org/10.1111/j.1365-3040.2009.01926.x
  21. Beauregard PB, Chai Y, Vlamakis H, Losick R, Kolter R. Bacillus subtilis biofilm induction by plant polysaccharides. Proc Natl Acad Sci. 2013;110(17):E1621-E30. https://doi.org/10.1073/pnas.1218984110
  22. Lebeis SL, Paredes SH, Lundberg DS, Breakfield N, Gehring J, McDonald M, et al. Salicylic acid modulates colonization of the root microbiome by specific bacterial taxa. Science. 2015;349(6250):860-64. https://doi.org/10.1126/science.aaa8764
  23. Zhalnina K, Louie KB, Hao Z, Mansoori N, Da Rocha UN, Shi S, et al. Dynamic root exudate chemistry and microbial substrate preferences drive patterns in rhizosphere microbial community assembly. Nat Microbiol. 2018;3(4):470-80. https://doi.org/10.1038/s41564-018-0129-3

Downloads

Download data is not yet available.