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Plant Science Today (PST)

Research Articles

Vol. 12 No. Sp2 (2025): Current Trends in Plant Science and Microbiome for Sustainability

Effects of exogenous proline treatment on antioxidant and biochemical parameters in Lepidium sativum L. plants cultivated in a water-stressed condition

DOI
https://doi.org/10.14719/pst.5560
Submitted
5 October 2024
Published
30-03-2025

Abstract

Proline, an organic compatible solute that acts as an osmoprotectant, is a crucial part of many plant's responses to water stress. In the current study, the impact of proline on enzymatic, non-enzymatic antioxidant and biochemical parameters in Lepidium sativum L. growing under water stress was examined. Plants were raised in controlled environments with a temperature of 25°C 16 h of light and 8 h of darkness. The concentrations of 50 ?g/L, 100 ?g/L and 250 ?g/ L proline were standardized and applied to plants grown under different water potential of -0.01?w MPa, -0.02?w MPa and -0.03?w MPa through foliar spray. After 35, 75 and 110 days of plant growth, the enzymatic antioxidants catalase, peroxidase, superoxide dismutase, ascorbate peroxidase, glutathione peroxidase, glutathione-s-transferase activity and non-enzymatic antioxidants like phenols, ascorbic acid, tocopherol, flavonoid content, were measured. The foliar application of proline increased enzymatic and non-enzymatic antioxidant assay activity in stressed growing plants compared to control plants. However, lipid peroxidation was reduced in water stressed plant due to proline application. The present study investigated that proline played a significant role in overcoming water stress in L. sativum. Moreover, this study supported that L. sativum plant copes with water stress by foliar application of proline.

References

  1. Zhang Y, Zhang Y, Mei Y, Zou R, Niu L, Dong S. Reactive oxygen species enlightened therapeutic strategy for oral and maxillofacial diseases-art of destruction and reconstruction. Biomed. 2022;10(11):2905. https://doi.org/10.3390/biomedicines10112905
  2. Kaur N, Kumar R, Alhan S, Sharma H, Singh N, Yogi R, et al. Lycium shawii mediated green synthesis of silver nanoparticles, characterization and assessments of their phytochemical, antioxidant, antimicrobial properties. Inorg Chem Commun. 2024;159:111735. https://doi.org/10.1016/j.inoche.2023.111735
  3. Razi K, Muneer S. Drought stress-induced physiological mechanisms, signalling pathways and molecular response of chloroplasts in common vegetable crops. Crit Rev Biotechnol. 2021;12:1–40.
  4. Tanveer M, Shahzad B, Sharma A, Khan EA. 24-Epibrassinolide application in plants: An implication for improving drought stress tolerance in plants. Plant Physiol Biochem. 2019;135:295–303. https://doi.org/10.1016/j.plaphy.2018.12.013
  5. Khadka K, Earl HJ, Raizada MN, Navabi A. A physio-morphological trait-based approach for breeding drought tolerant wheat. Front Plant Sci. 2020;11:715. https://doi.org/10.3389/fpls.2020.00715
  6. Martins J, Monteiro P, Pinto G, Canhoto J. Hybridization assays in strawberry tree toward the identification of plants displaying increased drought tolerance. Forests. 2021;12(2):148. https://doi.org/10.3390/f12020148
  7. Devireddy AR, Zandalinas SI, Fichman Y, Mittler R. Integration of reactive oxygen species and hormone signalling during abiotic stress. Plant J. 2021;105(2):459–76. https://doi.org/10.1111/tpj.15010
  8. Sahariah P, Bora J, Malik S, Syiem D, Bhan S, Hussain A, et al. Therapeutic potential of Dillenia indica L. in attenuating hyperglycemia-induced oxidative stress and apoptosis in alloxan-administered diabetic mice. Front Biosci (Landmark Ed). 2023;28(5):105.
  9. https://doi.org/10.31083/j.fbl2805105
  10. Rawat N, Singla?Pareek SL, Pareek A. Membrane dynamics during individual and combined abiotic stresses in plants and tools to study the same. Physiol Plant. 2021;171(4):653–56. https://doi.org/10.1111/ppl.13217
  11. Rabbani G, Choi I. Roles of osmolytes in protein folding and aggregation in cells and their biotechnological applications. Int J Biol Macromol. 2018;109:483–91. https://doi.org/10.1016/j.ijbiomac.2017.12.100
  12. Wani SH, Kumar V, Khare T, Guddimalli R, Parveda M, Solymosi K, et al. Engineering salinity tolerance in plants: progress and prospects. Planta. 2020;251:1–29. https://doi.org/10.1007/s00425-020-03366-6
  13. Suprasanna P, Nikalje GC, Rai AN. Osmolyte accumulation and implications in plant abiotic stress tolerance. In: Osmolytes and plants acclimation to changing environment: Emerging omics technologies. New Delhi: Springer, India; 2016. p. 1–12 https://doi.org/10.1007/978-81-322-2616-1_1
  14. Dikilitas M, Simsek E, Roychoudhury A. Role of proline and glycine betaine in overcoming abiotic stresses. In: Roychoudhury A, Tripathi DK, Editors. Protective chemical agents in the amelioration of plant abiotic stress: Biochemical and molecular perspectives. John Wiley and Sons; 2020. p. 1–23 https://doi.org/10.1002/9781119552154.ch1
  15. Ighodaro OM, Akinloye OA. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Bull Alexandria Fac. 2018;54(4):287–93. https://doi.org/10.1016/j.ajme.2017.09.001
  16. Srivastava RP, Kumar S, Singh L, Madhukar M, Singh N, Saxena G, et al. Major phenolic compounds, antioxidant, antimicrobial and cytotoxic activities of Selinum carvifolia (L.) collected from different altitudes in India. Front Nutr. 2023;10:1180225. https://doi.org/10.3389/fnut.2023.1180225
  17. Thakur A, Singh S, Dulta K, Singh N, Ali B, Hafeez A, et al. Nutritional evaluation, phytochemical makeup, antibacterial and antioxidant properties of wild plants utilized as food by the Gaddis-a tribal tribe in the Western Himalayas. Front Agron. 2022;4:1010309. https://doi.org/10.3389/fagro.2022.1010309
  18. Hasanuzzaman M, Anee TI, Bhuiyan TF, Nahar K, Fujita M. Emerging role of osmolytes in enhancing abiotic stress tolerance in rice. In: Advances in rice research for abiotic stress tolerance. Woodhead Publishing; 2019. p. 677–708 https://doi.org/10.1016/B978-0-12-814332-2.00033-2
  19. Singh S, Thakur A, Puri S. Impact of proline, glycine betaine and mannitol application on Lepidium sativum L. Plants grown under abiotic stress conditions (water stress). Plant Arch. 2020a;20(2):8671–89.
  20. Singh N, Jiwani G, Rocha LS, Mazaheri R. Bioagents and volatile organic compounds: An emerging control measures for rice bacterial diseases. In: Bacterial diseases of rice and their management. Apple Academic Press; 2023. p. 255–74 https://doi.org/10.1201/9781003331629-16
  21. Dixit P, Singh N, Singh L, Srivastava RP, Pandey S, Singh S, et al. Screening for the biochemical profile and biological activity in Cephalotaxus and Taxus collected from north-eastern Himalayas. ACS Agric Sci Technol. 2023;3(8):694–700. https://doi.org/10.1021/acsagscitech.3c00126
  22. Sharma V, Jambaladinni K, Singh N, Mishra N, Kumar A, Kumar R. Understanding environmental associated abiotic stress response in plants under changing climate. In: Molecular response and genetic engineering for stress in plants, Volume 1: Abiotic stress. Bristol, UK: IOP Publishing; 2022. p. 1-1 – 1?26 https://doi.org/10.1088/978-0-7503-4921-5ch1
  23. Kaur D, Grewal SK, Kaur J, Singh S. Differential proline metabolism in vegetative and reproductive tissues determine drought tolerance in chickpea. Biol Plant. 2017; 61(2):359–66. https://doi.org/10.1007/s10535-016-0695-2
  24. Li C, Wang Z, Nong Q, Lin L, Xie J, Mo Z, et al. Physiological changes and transcriptome profiling in Saccharum spontaneum L. leaf under water stress and re-watering conditions. Sci Rep. 2021;11(1):5525. https://doi.org/10.1038/s41598-021-85072-1
  25. Sahu N, Singh N, Arya KR, Reddy SS, Rai AK, Shukla V, et al. Assessment of the dual role of Lyonia ovalifolia (Wall.) Drude in inhibiting AGEs and enhancing GLUT4 translocation through LC-ESI-QTOF-MS/MS determination and in silico studies. Front Pharmacol. 2023;14:1073327. https://doi.org/10.3389/fphar.2023.1073327
  26. Ghaffari H, Tadayon MR, Nadeem M, Cheema M, Razmjoo J. Proline-mediated changes in antioxidant enzymatic activities and the physiology of sugar beet under drought stress. Acta Physiol Plant. 2019;41:1–3. https://doi.org/10.1007/s11738-019-2815-z
  27. Coelho DG, Marinato CS, De Matos LP, de Andrade HM, da Silva VM, Santos-Neves PH, et al. Is arsenite more toxic than arsenate in plants?. Ecotoxicol. 2020;29:196–202. https://doi.org/10.1007/s10646-019-02152-9
  28. Yang X, Lu M, Wang Y, Wang Y, Liu Z, Chen S. Response mechanism of plants to drought stress. Horticulturae. 2021;7(3):50. https://doi.org/10.3390/horticulturae7030050
  29. Han G, Lu C, Guo J, Qiao Z, Sui N, Qiu N, Wang B. C2H2 zinc finger proteins: master regulators of abiotic stress responses in plants. Front Plant Sci. 2020;11:115. https://doi.org/10.3389/fpls.2020.00115
  30. Dar MI, Naikoo MI, Rehman F, Naushin F, Khan FA. Proline accumulation in plants: Roles in stress tolerance and plant development. In: Osmolytes and plants acclimation to changing environment: emerging omics technologies. Springer, New Delhi; 2016. p. 155–66
  31. https://doi.org/10.1007/978-81-322-2616-1_9
  32. Sharma L, Roy S, Satya P, Alam NM, Goswami T, Barman D, et al. Exogenous ascorbic acid application ameliorates drought stress through improvement in morpho-physiology, nutrient dynamics, stress metabolite production and antioxidant activities recovering cellulosic fibre production in jute (Corchorus olitorius L.). Ind Crops Prod. 2024;217:118808.
  33. https://doi.org/10.1016/j.indcrop.2024.118808
  34. Semida WM, Abdelkhalik A, Rady MO, Marey RA, Abd El-Mageed TA. Exogenously applied proline enhances growth and productivity of drought stressed onion by improving photosynthetic efficiency, water use efficiency and up-regulating osmoprotectants. Sci Hortic. 2020;272:109580.
  35. https://doi.org/10.1016/j.scienta.2020.109580
  36. Kohli SK, Gautum V, Bali S, Kaur P, Sharma A, Bakshi P, et al. Emerging trends of proline metabolism in abiotic stress management. In: Improving abiotic stress tolerance in plants. CRC Press; 2020. p. 177–202 https://doi.org/10.1201/9780429027505-10
  37. Ghosh UK, Islam MN, Siddiqui MN, Cao X, Khan MA. Proline, a multifaceted signalling molecule in plant responses to abiotic stress: understanding the physiological mechanisms. Plant Biol. 2022;24(2):227–39. https://doi.org/10.1111/plb.13363
  38. Molla MR, Ali MR, Hasanuzzaman M, Al-Mamun MH, Ahmed A, Nazim-Ud-Dowla MA, et al. Exogenous proline and betaine-induced upregulation of glutathione transferase and glyoxalase I in lentil (Lens culinaris) under drought stress. Not Bot Horti Agrobot Cluj Napoca. 2014;42(1):73–80. https://doi.org/10.15835/nbha4219324
  39. Patade VY, Lokhande VH, Suprasanna P. Exogenous application of proline alleviates
  40. salt induced oxidative stress more efficiently than glycine betaine in sugarcane-cultured cells. Sugar Technol. 2014;16:22–29. https://doi.org/10.1007/s12355-013-0261-6
  41. Habib N, Akram MS, Javed MT, Azeem M, Ali Q, Shaheen HL, Ashraf M. Nitric oxide regulated improvement in growth and yield of rice plants grown under salinity stress: antioxidant defense system. Appl Ecol Environ Res. 2016;14(5):91–105. https://doi.org/10.15666/aeer/1405_091105
  42. Moradi P, Mahdavi A, Khoshkam M, Iriti M. Lipidomics unravels the role of leaf lipids in thyme plant response to drought stress. Int J Mol Sci. 2017;18(10):2067. https://doi.org/10.3390/ijms18102067
  43. Carvalho M, Gouvinhas I, Castro I, Matos M, Rosa E, Carnide V, Barros A. Drought stress effect on polyphenolic content and antioxidant capacity of cowpea pods and seeds. J Agron Crop Sci. 2021;207(2):197–207. https://doi.org/10.1111/jac.12454
  44. Rajak BK, Rani P, Singh N, Singh DV. Sequence and structural similarities of ACCase protein of Phalaris minor and wheat: An insight to explain herbicide selectivity. Front Plant Sci. 2023;13:1056474. https://doi.org/10.3389/fpls.2022.1056474
  45. Nakhaie A, Habibi G, Vaziri A. Exogenous proline enhances salt tolerance in acclimated Aloe vera by modulating photosystem II efficiency and antioxidant defense. S Afr J Bot. 2022;147:1171–80. https://doi.org/10.1016/j.sajb.2020.06.005
  46. Nakabayashi R, Mori T, Saito K. Alternation of flavonoid accumulation under drought stress in Arabidopsis thaliana. Plant Signal Behav. 2014;9:29518. https://doi.org/10.4161/psb.29518
  47. Swarcewicz B, Sawikowska A, Marczak U, Uczak M, Stobiecki M. Effect of drought
  48. stress on metabolite contents in barley recombinant inbred line population revealed by
  49. untargeted GC-MS profiling. Acta Physiol Plant. 2017;39:1–16.
  50. Wang X, Chen S, Shi X, Liu D, Zhao P, Lu Y, et al. Hybrid sequencing reveals insight into heat sensing and signaling of bread wheat. Plant J. 2019;98(6):1015–32. https://doi.org/10.1111/tpj.14299
  51. Jia H, Wang L, Li J, Sun P, Lu M, Hu J. Comparative metabolomics analysis reveals different metabolic responses to drought in tolerant and susceptible poplar species. Physiol Plant. 2020;168(3):531–46. https://doi.org/10.1111/ppl.13036
  52. Zhao M, Ren Y, Wei W, Yang J, Zhong Q, Li Z. Metabolite analysis of Jerusalem artichoke (Helianthus tuberosus L.) seedlings in response to polyethylene glycol-simulated drought stress. Int J Mol Sci. 2021;22(7):3294. https://doi.org/10.3390/ijms22073294
  53. Slama I, Tayachi S, Jdey A, Rouached A, Abdelly C. Differential response to water deficit stress in alfalfa (Medicago sativa) cultivars: Growth, water relations, osmolyte
  54. accumulation and lipid peroxidation. Afr J Biotechnol. 2011;10(72):16250–59.
  55. Demiralay M, Altuntas C, Sezgin A,Terzi R, Kadioglu A. Application of proline to root medium is more effective for amelioration of photosynthetic damages as compared to foliar spraying or seed soaking in maize seedlings under short-term drought. Turkish J Biol. 2017;41(4):649–60. https://doi.org/10.3906/biy-1702-19
  56. Ali Q, Anwar F, Ashraf M, Saari N, Perveen R. Ameliorating effects of exogenously applied proline on seed composition, seed oil quality and oil antioxidant activity of maize (Zea mays L.) under drought stress. Int J Mol Sci. 2013;14:818–35. https://doi.org/10.3390/ijms14010818
  57. Chauhan A, Chauhan M, Sethi M, Bodhe A, Tomar A, Shikha, et al. Application of flower wastes to produce valuable products. In: Valorization of biomass wastes for environmental sustainability: Green practices for the rural circular economy. Springer, Cham.; 2024 Mar 15. 251–68 https://doi.org/10.1007/978-3-031-52485-1_14

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