Effects of exogenous proline treatment on antioxidant and biochemical parameters in Lepidium sativum L. plants cultivated in a water-stressed condition

S Singh; A Thakur; K Dulta; A J Sharma; N Kumar; S Dwivedi; M D Choudhury; K Verma; N Singh

doi:10.14719/pst.5560

Authors

S Singh Department of Biosciences, University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali 140 413, Punjab, India https://orcid.org/0000-0002-3718-6086
A Thakur Department of Botany, Shoolini Institute of Life Sciences & Business Management, Solan 173 229, Himachal Pradesh, India https://orcid.org/0000-0001-5925-2227
K Dulta Department of Food Technology, School of Applied and Life Sciences, Uttaranchal University, Dehradun 248 007, Uttarakhand, India https://orcid.org/0000-0002-3661-5205
A J Sharma Department of Biosciences, Career Point University, Hamirpur 176 041, Himachal Pradesh, India https://orcid.org/0000-0001-8438-5451
N Kumar School of Biotechnology, Jawaharlal Nehru University, New Delhi 110 067, India https://orcid.org/0000-0001-8129-6496
S Dwivedi Department of Chemistry, Dayanand Brijendra Swarup (Post-Graduate) College, Dehradun 248 001, Uttarakhand, India https://orcid.org/0000-0002-5807-3929
M D Choudhury Department of Biosciences, University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali 140 413, Punjab, India https://orcid.org/0000-0003-1237-1370
K Verma Department of Applied Sciences, Shri Rawatpura university, Dhaneli, Raipur- 492 016, Chhattisgarh, India https://orcid.org/0009-0003-1291-3536
N Singh Department of Plant Protection, Faculty of Agricultural Sciences, Shree Guru Gobind Singh Tricentenary University, Gurugram 122 505, Haryana, India https://orcid.org/0000-0002-7779-174X

DOI:

https://doi.org/10.14719/pst.5560

Keywords:

catalase, osmolyte, peroxidase, proline, superoxide dismutase, water stress

Abstract

Proline, an organic compatible solute that acts as an osmoprotectant, is a crucial part of many plant's responses to water stress. In the current study, the impact of proline on enzymatic, non-enzymatic antioxidant and biochemical parameters in Lepidium sativum L. growing under water stress was examined. Plants were raised in controlled environments with a temperature of 25°C 16 h of light and 8 h of darkness. The concentrations of 50 ?g/L, 100 ?g/L and 250 ?g/ L proline were standardized and applied to plants grown under different water potential of -0.01?w MPa, -0.02?w MPa and -0.03?w MPa through foliar spray. After 35, 75 and 110 days of plant growth, the enzymatic antioxidants catalase, peroxidase, superoxide dismutase, ascorbate peroxidase, glutathione peroxidase, glutathione-s-transferase activity and non-enzymatic antioxidants like phenols, ascorbic acid, tocopherol, flavonoid content, were measured. The foliar application of proline increased enzymatic and non-enzymatic antioxidant assay activity in stressed growing plants compared to control plants. However, lipid peroxidation was reduced in water stressed plant due to proline application. The present study investigated that proline played a significant role in overcoming water stress in L. sativum. Moreover, this study supported that L. sativum plant copes with water stress by foliar application of proline.

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