Evaluating growth and biochemistry of Westiellopsis prolifica in response to Malathion
DOI:
https://doi.org/10.14719/pst.2019.6.4.622Keywords:
Cyanobacterium, Organophosphate, MalathionAbstract
The present endeavour was aimed to investigate the chronic response of Westiellopsis prolifica to an organophosphate insecticide malathion at different concentrations 30, 60 and 90ppm. The influence of malathion on growth (biomass), pigments (chlorophyll-a, carotenoid), release of metabolites such as protein and carbohydrate was analysed for a period of 16 days under aseptic laboratory conditions. Results revealed enhancement in chlorophyll-a production at 30ppm on 4th day (p?0.05) and 8th day (p?0.05), from the day of inoculation. On the other hand, there was a significant decrease in the carotenoid, protein and carbohydrate content with increase in malathion concentration in a time and dose dependent manner. However, a little but insignificant increase in biomass was recorded on the 4th day at 30ppm concentration over the control. The study revealed that the reduction in biomass, protein and carbohydrate content with the increase of malathion concentrations was an indication of its toxicity to the test cyanobacterium which is one of the natural biofertilizers in the rice field ecosystem.
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References
1. Ware GW. Pesticides: theory and application. Freeman, Newyork. 1983.
2. Maly M, Rubber E. Bull. Environ. Contam. Toxicol. 1983; 30:464. https://doi.org/10.1007/BF01610161
3. Bambaradeniya CNB, Amerasinghe FP. Biodiversity Associated with the Rice Field Agroecosystem in Asian Countries: A Brief Review. Colombo, Sri Lanka: International Water Management Institute Working Paper. 2003. p.63.
4. Singh DP, Khattar JIS, Guota M, Kaur G. Evaluation of toxicological impact of cartap hydrochloride on some physiological activities of a non-heterocystous cyanobacterium Leptolyngbya foveolarum. Pestic Biochem Physiol. 2014;110.63-70. https://doi.org/10.1016/j.pestbp.2014.03.002
5.Patil VK, David M . Behaviour and respiratory dysfunction as an index of Malathion toxicity in the freshwater fish", Labeo rohita (Hamilton)", Turk J Fish Aqua Sci. 2008; 8:233.
6. Anton FA, Laborda E, Laborda P, Ramos E. Carbofuran acute toxicity to freshwater algae and fish. Bull Environ Contam Toxicol. 1993; 50: 400. https://doi.org/10.1007/BF00197199
7. Tiwari O, Prasanna R, Yadav A, Dhar WD , Singh P. Growth potential and biocide tolerance of non- heterocystous filamentous cyanobacterial isolates from rice fields of Uttar Pradesh. India,Biol Fertil Soils. 2001; 34: 291. https://doi.org/10.1007/s003740100402
8. Singh DP, Khattar JIS, Kaur M, Kaur G, Gupta M, Singh Y. Anilofostoleranceand its mineralization by the cyanobacterium Synechocystis sp. strain PUPCCC 64.PLoS ONE. 2013; 8: 1-10. https://doi.org/10.1371/journal.pone.0053445
9. Venkataraman GS. Blue green algae: a possible remedy to nitrogen scarcity. Curr Sci. 1981; 50:253-56.
10. Nayak S, Prasanna R. Soil pH and its role in cyanobacterial abundance and diversity in rice field soils. Appl Ecol Environ Res. 2007; 5: 103-13. https://doi.org/10.15666/aeer/0502_103113
11. Singh OA, Tiwari GO. Part 3: An account of heterocystous stigonematalean cyanobacterial biodiversity of North East Region of India. J. Indian bot. Soc. 2011; 90(3&4): 200-06.
12. Singh RN. The role of Blue Green Algae in Nitrogen economy of Indian agriculture. Indian Council of Agricultural Research and Publication. New Delhi. 1961.
13. Irisarri P, Gonnet S, Monza J. Cyanobacteria in Uruguayan rice fields: Diversity, nitrogen fixing ability and tolerance to herbicides and combined nitrogen. J. Biotechnol. 2001; 91: 95-103. https://doi.org/10.1016/S0168-1656(01)00334-0
14. Singh NK, Dhar DW. Cyanobacterial reclamation of salt-affected soil. In: Genetic engineering, biofertilisation, soil quality and organic farming (Ed. by E. Lichtfouse). Springer Science þ Business Media B.V., Dordrecht, the Netherlands.2010; 243-75. https://doi.org/10.1007/978-90-481-8741-6_9
15. Pankratz FB, Doebel C, Farenhorst A, Goldborough LG. Interaction between algae (Selenastrum capricornutum) and pesticides. Implication for managing constructed wetlands for pesticides removal. J Env Sci Health B. 2003;38:147-55. https://doi.org/10.1081/PFC-120018445
16. Kumar NJI, Khusboo B, Rita K, Jeegna B. Biochemical and enzymatic investigation of Westiellopsis prolifica Janet in response to mercury stress.Int J Adv Res. 2015; 3(7): 1018-29.
17. Gupta K, Baruah PP. Effect of lambdacyhalothrin on Calothrix sp. (GUEco 1001), an authchthonous cyanobacterium of rice fields of Brahmaputra floodplain. Environ Sci Pollut Res.2015; 22:18554-60. https://doi.org/10.1007/s11356-015-5181-4
18. Mackinney G. Absorption of light by chlorophyll solution. J Biol Chem.1941;140:315-22.
19. Myers J, Kratz WA. Relationship between pigments content and photosynthetic characteristics in a blue green algae. J Gen Physiol. 1955; 39:11-21. https://doi.org/10.1085/jgp.39.1.11
20. Lowry OH, Rosenbrough NH, Farr AL, and Randall RJ. Protein measurements with folinphenol reagent. J Biol Chem. 1951; 193: 265-75.
21. Spiro RG. Analysis of sugars found in glycoprotein. Methods in Enzymol. 1966; 8:3-26. https://doi.org/10.1016/0076-6879(66)08005-4
22. Prosperi C, Luna C, Valiente EF. Influence of pH light intensity and oxygen on the short term effect of ammonium on nitrogenase activity of cyanobacteria from rice fields. Environ Exp. 1993; 33(4): 545-52. https://doi.org/10.1016/0098-8472(93)90029-F
23. Ningthoujam M, Satyendra K, Khalid H, Tasneem F. Biochemical Analysis of Anabaena variabilis Exposed to Malathion Pesticide with Special Reference to Oxidative Stress and Osmolytes. International Journal of Innovative Research in Science, Engineering and Technology. 2003;2(10):5403-20.
24. Galhano V, Peixoto F, Gomes-Laranjo J, Fernandez-Valiente E. Differential effect of bentazon and molinate on Anabaena cylindrica, an autochthonous cyanobacterium of Portuguese rice field agro ecosystem. Water Air Soil Pollut. 2009; 197(1-4):211-22.
https://doi.org/10.1007/s11270-008-9804-y
25. Eullaffroy P, Vernet G. The F684/F735 chlorophyll fluorescence ratio: a potential tool for rapid detection and determination of herbicide phytotoxicity in algae.Water Res. 2003;37(9):1983-90. https://doi.org/10.1016/S0043-1354(02)00621-8
26. Mohapatra PK, Patra S, Samantaray PK, Mohanty RC . Effect of the pyrethroid insecticide cypermethrin on photosynthetic pigments of the Cyanobacterium Anabaena doliolum Bhar. Pol. J. Environ. Stud. 2003;12: 207-12.
27. Tandon RS, Lal R, Rao N, VVS. Interaction of endosulfan and malathion with blue-green algae Anabaena and Aulosira fertilissima. Environ Poll. 1988; 52: 1-9. https://doi.org/10.1016/0269-7491(88)90103-0
28. Yamamoto Y, Tsukada H. Measurement of in situ specific growth rates of microcystis (cyanobacteria) from the frequency of dividing cells. J Phy. 2009; 45: 1003-09. https://doi.org/10.1111/j.1529-8817.2009.00723.x
29. Schagerl M, Muller B. Acclimation of chlorophyll a and carotenoid levels to different irradiances in four freshwater cyanobacteria. J Plant Physiol. 2006;163: 709-16. https://doi.org/10.1016/j.jplph.2005.09.015
30. Kapoor K, Leenta A . Observations on growth responses of cyanobacteria under the influence of herbicides. Poll Res. 1996; 15(4): 343-51.
31. Dowidar SMA, Osman MEH, Naggar AHEI, Khalefa AE. Effect of butachlor and thiobencarb herbicides on protein content and profile and some enzyme activities of Nostoc muscorum. J Genet Eng Biotechnol. 2010; 8:89-95.
32. Chai JK, Chung YS. Physiological effects of 2,4-dichlorophenoxyacetic acid (2,4-D) on Chlorella ellipsoidea. MisaengmulHakhoe Chi. (in Korean). 1975; 13:101-08.
33. Kumar N, Bora A, Kumar R, Kaur M. Differential Effects of Agricultural Pesticides Endosulfan and Tebuconazole on Photosynthetic pigments, Metabolism and Assimilating Enzymes of Three Heterotrophic, Filamentous Cyanobacteria. J. Biol. Environ. Sci. 2012; 6(16): 67-75.
34. Averamova S, Rossler M .Effect of various temperatures on some physiological-biochemical induces during the light phase of the life cycle of Scenedesmus sp. Appl Microbiol. 1975; 5: 115-20.
35. Manikar N, Kumar S, Habib K. and Fatma T. Biochemical analysis of Anabaena variabilis exposed to malathion pesticide with special references to oxidative stress and osmolyte. Int J Innov Res Sci Eng Technol. 2013; 2:5403-20.
2. Maly M, Rubber E. Bull. Environ. Contam. Toxicol. 1983; 30:464. https://doi.org/10.1007/BF01610161
3. Bambaradeniya CNB, Amerasinghe FP. Biodiversity Associated with the Rice Field Agroecosystem in Asian Countries: A Brief Review. Colombo, Sri Lanka: International Water Management Institute Working Paper. 2003. p.63.
4. Singh DP, Khattar JIS, Guota M, Kaur G. Evaluation of toxicological impact of cartap hydrochloride on some physiological activities of a non-heterocystous cyanobacterium Leptolyngbya foveolarum. Pestic Biochem Physiol. 2014;110.63-70. https://doi.org/10.1016/j.pestbp.2014.03.002
5.Patil VK, David M . Behaviour and respiratory dysfunction as an index of Malathion toxicity in the freshwater fish", Labeo rohita (Hamilton)", Turk J Fish Aqua Sci. 2008; 8:233.
6. Anton FA, Laborda E, Laborda P, Ramos E. Carbofuran acute toxicity to freshwater algae and fish. Bull Environ Contam Toxicol. 1993; 50: 400. https://doi.org/10.1007/BF00197199
7. Tiwari O, Prasanna R, Yadav A, Dhar WD , Singh P. Growth potential and biocide tolerance of non- heterocystous filamentous cyanobacterial isolates from rice fields of Uttar Pradesh. India,Biol Fertil Soils. 2001; 34: 291. https://doi.org/10.1007/s003740100402
8. Singh DP, Khattar JIS, Kaur M, Kaur G, Gupta M, Singh Y. Anilofostoleranceand its mineralization by the cyanobacterium Synechocystis sp. strain PUPCCC 64.PLoS ONE. 2013; 8: 1-10. https://doi.org/10.1371/journal.pone.0053445
9. Venkataraman GS. Blue green algae: a possible remedy to nitrogen scarcity. Curr Sci. 1981; 50:253-56.
10. Nayak S, Prasanna R. Soil pH and its role in cyanobacterial abundance and diversity in rice field soils. Appl Ecol Environ Res. 2007; 5: 103-13. https://doi.org/10.15666/aeer/0502_103113
11. Singh OA, Tiwari GO. Part 3: An account of heterocystous stigonematalean cyanobacterial biodiversity of North East Region of India. J. Indian bot. Soc. 2011; 90(3&4): 200-06.
12. Singh RN. The role of Blue Green Algae in Nitrogen economy of Indian agriculture. Indian Council of Agricultural Research and Publication. New Delhi. 1961.
13. Irisarri P, Gonnet S, Monza J. Cyanobacteria in Uruguayan rice fields: Diversity, nitrogen fixing ability and tolerance to herbicides and combined nitrogen. J. Biotechnol. 2001; 91: 95-103. https://doi.org/10.1016/S0168-1656(01)00334-0
14. Singh NK, Dhar DW. Cyanobacterial reclamation of salt-affected soil. In: Genetic engineering, biofertilisation, soil quality and organic farming (Ed. by E. Lichtfouse). Springer Science þ Business Media B.V., Dordrecht, the Netherlands.2010; 243-75. https://doi.org/10.1007/978-90-481-8741-6_9
15. Pankratz FB, Doebel C, Farenhorst A, Goldborough LG. Interaction between algae (Selenastrum capricornutum) and pesticides. Implication for managing constructed wetlands for pesticides removal. J Env Sci Health B. 2003;38:147-55. https://doi.org/10.1081/PFC-120018445
16. Kumar NJI, Khusboo B, Rita K, Jeegna B. Biochemical and enzymatic investigation of Westiellopsis prolifica Janet in response to mercury stress.Int J Adv Res. 2015; 3(7): 1018-29.
17. Gupta K, Baruah PP. Effect of lambdacyhalothrin on Calothrix sp. (GUEco 1001), an authchthonous cyanobacterium of rice fields of Brahmaputra floodplain. Environ Sci Pollut Res.2015; 22:18554-60. https://doi.org/10.1007/s11356-015-5181-4
18. Mackinney G. Absorption of light by chlorophyll solution. J Biol Chem.1941;140:315-22.
19. Myers J, Kratz WA. Relationship between pigments content and photosynthetic characteristics in a blue green algae. J Gen Physiol. 1955; 39:11-21. https://doi.org/10.1085/jgp.39.1.11
20. Lowry OH, Rosenbrough NH, Farr AL, and Randall RJ. Protein measurements with folinphenol reagent. J Biol Chem. 1951; 193: 265-75.
21. Spiro RG. Analysis of sugars found in glycoprotein. Methods in Enzymol. 1966; 8:3-26. https://doi.org/10.1016/0076-6879(66)08005-4
22. Prosperi C, Luna C, Valiente EF. Influence of pH light intensity and oxygen on the short term effect of ammonium on nitrogenase activity of cyanobacteria from rice fields. Environ Exp. 1993; 33(4): 545-52. https://doi.org/10.1016/0098-8472(93)90029-F
23. Ningthoujam M, Satyendra K, Khalid H, Tasneem F. Biochemical Analysis of Anabaena variabilis Exposed to Malathion Pesticide with Special Reference to Oxidative Stress and Osmolytes. International Journal of Innovative Research in Science, Engineering and Technology. 2003;2(10):5403-20.
24. Galhano V, Peixoto F, Gomes-Laranjo J, Fernandez-Valiente E. Differential effect of bentazon and molinate on Anabaena cylindrica, an autochthonous cyanobacterium of Portuguese rice field agro ecosystem. Water Air Soil Pollut. 2009; 197(1-4):211-22.
https://doi.org/10.1007/s11270-008-9804-y
25. Eullaffroy P, Vernet G. The F684/F735 chlorophyll fluorescence ratio: a potential tool for rapid detection and determination of herbicide phytotoxicity in algae.Water Res. 2003;37(9):1983-90. https://doi.org/10.1016/S0043-1354(02)00621-8
26. Mohapatra PK, Patra S, Samantaray PK, Mohanty RC . Effect of the pyrethroid insecticide cypermethrin on photosynthetic pigments of the Cyanobacterium Anabaena doliolum Bhar. Pol. J. Environ. Stud. 2003;12: 207-12.
27. Tandon RS, Lal R, Rao N, VVS. Interaction of endosulfan and malathion with blue-green algae Anabaena and Aulosira fertilissima. Environ Poll. 1988; 52: 1-9. https://doi.org/10.1016/0269-7491(88)90103-0
28. Yamamoto Y, Tsukada H. Measurement of in situ specific growth rates of microcystis (cyanobacteria) from the frequency of dividing cells. J Phy. 2009; 45: 1003-09. https://doi.org/10.1111/j.1529-8817.2009.00723.x
29. Schagerl M, Muller B. Acclimation of chlorophyll a and carotenoid levels to different irradiances in four freshwater cyanobacteria. J Plant Physiol. 2006;163: 709-16. https://doi.org/10.1016/j.jplph.2005.09.015
30. Kapoor K, Leenta A . Observations on growth responses of cyanobacteria under the influence of herbicides. Poll Res. 1996; 15(4): 343-51.
31. Dowidar SMA, Osman MEH, Naggar AHEI, Khalefa AE. Effect of butachlor and thiobencarb herbicides on protein content and profile and some enzyme activities of Nostoc muscorum. J Genet Eng Biotechnol. 2010; 8:89-95.
32. Chai JK, Chung YS. Physiological effects of 2,4-dichlorophenoxyacetic acid (2,4-D) on Chlorella ellipsoidea. MisaengmulHakhoe Chi. (in Korean). 1975; 13:101-08.
33. Kumar N, Bora A, Kumar R, Kaur M. Differential Effects of Agricultural Pesticides Endosulfan and Tebuconazole on Photosynthetic pigments, Metabolism and Assimilating Enzymes of Three Heterotrophic, Filamentous Cyanobacteria. J. Biol. Environ. Sci. 2012; 6(16): 67-75.
34. Averamova S, Rossler M .Effect of various temperatures on some physiological-biochemical induces during the light phase of the life cycle of Scenedesmus sp. Appl Microbiol. 1975; 5: 115-20.
35. Manikar N, Kumar S, Habib K. and Fatma T. Biochemical analysis of Anabaena variabilis exposed to malathion pesticide with special references to oxidative stress and osmolyte. Int J Innov Res Sci Eng Technol. 2013; 2:5403-20.
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06-10-2019
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Dutta J, Baruah PP. Evaluating growth and biochemistry of Westiellopsis prolifica in response to Malathion. Plant Sci. Today [Internet]. 2019 Oct. 6 [cited 2024 Nov. 21];6(4):505-11. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/622
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