Antibiofilm and anti-quorum properties of ethanolic leaf extracts of Syzygium jambos and Psidium guajava and their gel formulation for wound healing applications
DOI:
https://doi.org/10.14719/pst.6365Keywords:
antibiofilm, anti-quorum, AgNPs, Psidium guajava, Syzygium jambos, wound healingAbstract
Most bacterial species today have evolved with time and gained resistance to a wide range of antibiotics, primarily due to formation of biofilms and ?lactamases. Many phytochemicals have been explored for their ability to inhibit bacterial biofilms. The present study sheds light on antibiofilm properties of two such plants viz. Psidium guajava and Syzygium jambos, of the Myrtaceae family. They were found to be effective against four different biofilm forming pathogens - Chromobacterium violaceum, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. Synergistic use of the plant extracts showed slightly better antibacterial activity than a single extract. Quorum sensing being one of the key factors required for biofilm formation, the isolate Chromobacterium violaceum was used as the indicator organism to study the anti-quorum properties of the plant extracts. At 10 mg/mL, ethanolic extract of S. jambos inhibited violacein pigment the most (78.84%) and therefore can be considered as a quorum sensing inhibitor (QSI). Since silver nanoparticles (AgNPs) have become increasingly significant in the field of drug delivery, they may be utilized to coat implants to avoid subsequent infections in patients who have had implant surgery and to reduce biofilm development in pathogens. In the present study, five gels were formulated using plant extracts and AgNPs, of which two showed promising results in wound healing assay. The non-toxic nature of the synthesized gels has been verified by studies on L-929 mouse fibroblast cell lines, which opens the door for their prospective application as topical treatments to accelerate the healing process in both acute and chronic wounds. Given that S. aureus and P. aeruginosa are the most commonly isolated bacteria from diabetic foot ulcers, the resulting gels can considerably curb the spread of infection and gangrene and thus prevent amputation.
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References
Paladini F, Pollini M. Antimicrobial silver nanoparticles for wound healing application: progress and future trends. Materials (Basel). 2019;12(16):2540. https://doi.org/10.3390/ma12162540
Dumaru R, Baral R, Shrestha LB. Study of biofilm formation and antibiotic resistance pattern of gram-negative Bacilli among the clinical isolates at BPKIHS, Dharan. BMC Res Notes. 2019;12(1):38. https://doi.org/10.1186/s13104-019-4084-8
Li J, Zhao X. Effects of quorum sensing on the biofilm formation and viable but non-culturable state. Food Res Int. 2020;137:109742. https://doi.org/10.1016/j.foodres.2020.109742
Samrot AV, Mohamed A, Faradjeva E, Si JL, Hooi SC, Arif A, et al. Mechanisms and impact of biofilms and targeting of biofilms using bioactive compounds—A review. Medicina (Kaunas). 2021;57(8):839. https://doi.org/10.3390/medicina57080839
Harika K, Shenoy VP, Narasimhaswamy N, Chawla K. Detection of biofilm production and its impact on antibiotic resistance profile of bacterial isolates from chronic wound infections. J Glob Infect Dis. 2020;12(3):129–34.
https://doi.org/10.4103/jgid.jgid_150_19
Shrestha LB, Bhattarai NR, Khanal B. Antibiotic resistance and biofilm formation among coagulase-negative staphylococci isolated from clinical samples at a tertiary care hospital of eastern Nepal. Antimicrob Resist Infect Control. 2017;6(1):89. https://doi.org/10.1186/s13756-017-0251-7
Basnet A, Tamang B, Shrestha MR, Shrestha LB, Rai JR, Maharjan R, et al. Assessment of four in vitro phenotypic biofilm detection methods in relation to antimicrobial resistance in aerobic clinical bacterial isolates. PLoS One. 2023;18(11):e0294646.
https://doi.org/10.1371/journal.pone.0294646
Khan MA, Celik I, Khan HM, Shahid M, Shahzad A, Kumar S, et al. Antibiofilm and anti-quorum sensing activity of Psidium guajava L. leaf extract: In vitro and in silico approach. PLoS One. 2023;18(12):e0295524. https://doi.org/10.1371/journal.pone.0295524
Burdu?el AC, Gherasim O, Grumezescu AM, Mogoant? L, Ficai A, Andronescu E.Biomedical applications of silver nanoparticles: An Up-to-Date overview. Nanomaterials (Basel). 2018;8(9):681. https://doi.org/10.3390/nano8090681
Xu L, Wang YY, Huang J, Chen CY, Wang ZX, Xie H. Silver nanoparticles: Synthesis, medical applications and biosafety. Theranostics. 2020;10(20):8996–9031. https://doi.org/10.7150/thno.45413
Swidan NS, Hashem YA, Elkhatib WF, Yassien MA. Antibiofilm activity of green synthesized silver nanoparticles against biofilm associated enterococcal urinary pathogens. Sci Rep. 2022;12(1):3869. https://doi.org/10.1038/s41598-022-07831-y
Bubonja-Šonje M, Kneževi? S, Abram M. Challenges to antimicrobial susceptibility testing of plant-derived polyphenolic compounds. Arh Hig Rada Toksikol. 2020;71(4):300–11. https://doi.org/10.2478/aiht-2020-71-3396
de Castro Melo P, Ferreira LM, Filho AN, Zafalon LF, Vicente HIG, de Souza V.Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis. Braz J Microbiol. 2013;44(1):119–24. https://doi.org/10.1590/S1517 83822013005000031
Brown HL, Van Vliet AHM, Betts RP, Reuter M. Tetrazolium reduction allows assessment of biofilm formation by Campylobacter jejuni in a food matrix model. J Appl Microbiol. 2013;115(5):1212–21. https://doi.org/10.1111/jam.12316
Samreen, Qais FA, Ahmad I. Anti-quorum sensing and biofilm inhibitory effect of some medicinal plants against gram-negative bacterial pathogens: in vitro and in silico investigations. Heliyon. 2022;8(10):e11113. https://doi.org/10.1016/j.heliyon.2022.e11113
Kuete V, BetrandTeponno R, Mbaveng AT, Tapondjou LA, Meyer JJM, Barboni L, et al. Antibacterial activities of the extracts, fractions and compounds from Dioscorea bulbifera. BMC Complement Altern Med. 2012;12:228. https://doi.org/10.1186/1472-6882-12-228
Basson A, Flemming LA, Chenia HY. Evaluation of adherence, hydrophobicity, aggregation and biofilm development of Flavobacterium johnsoniae-like isolates. Microb Ecol. 2008;55(1):1–14. https://doi.org/10.1007/s00248-007-9245-y
Rosenberg M. Bacterial adherence to hydrocarbons: a useful technique for studying cell surface hydrophobicity. FEMS Microbiol Lett. 1984;22(3):289–95. https://doi.org/10.1016/0378-1097(84)90026-0
Bose D, Chatterjee S. Biogenic synthesis of silver nanoparticles using guava (Psidium guajava) leaf extract and its antibacterial activity against Pseudomonas aeruginosa. Appl Nanosci. 2016;6(6):895–901. https://doi.org/10.1007/s13204-015-0496-5
Olojede SO, Lawal SK, Mahlangeni N, Shelembe B, Matshipi MN, Moodley R, et al. Synthesis and characterization of a conjugate of silver nanoparticles loaded with tenofovir disoproxil fumarate. Next Nanotechnol. 2024;5:100058. https://doi.org/10.1016/j.nxnano.2024.100058
Inamdar YM, Rane B, Jain A. Preparation and evaluation of beta sitosterol nanogel: A carrier design for targeted drug delivery system. Asian J Pharm Res Dev. 2018;6(3):81–87. https://doi.org/10.22270/ajprd.v6i3.390
Gerlier D, Thomasset N. Use of MTT colorimetric assay to measure cell activation. J Immunol Methods. 1986;94(1–2):57–63. https://doi.org/10.1016/0022-1759(86)90215-2
Bolla SR, Mohammed Al-SA, Yousuf Al-JR, Papayya BJ, Kanchi RP, Veeraraghavan VP, et al. In vitro wound healing potency of methanolic leaf extract of Aristolochia saccata is possibly mediated by its stimulatory effect on collagen-1 expression. Heliyon. 2019;5(5):e01648. https://doi.org/10.1016/j.heliyon.2019.e01648
Yahaya A, Ali M, El-Hassan FI, Jido BL. Antibacterial activity of guava (Psidium guajava L.) extracts on Staphylococcus aureus isolated from patients with urinary tract infections attending a tertiary-care hospital. Sci World J. 2019;14(1).
Kenmeni JF, Sifi I, Bisso BN, Kayoka-Kabongo PN, Tsopmene UJ, Dzoyem JP. Exploring medicinal plants for antimicrobial activity and synergistic effects with doxycycline against bacterial species. The Sci World J. 2024;2024:6238852. https://doi.org/10.1155/2024/6238852
Lima JL da C, Alves LR, Paz JNP da, Rabelo MA, Maciel MAV, Morais MMC de. Analysis of biofilm production by clinical isolates of Pseudomonas aeruginosa from patients with ventilator-associated pneumonia. Rev Bras Ter Intensiva. 2017;29(3):310. https://doi.org/10.5935/0103-507X.20170039
Mori S, Yamada A, Kawai K. Evaluation of the biofilm detection capacity of the Congo Red agar method for bovine mastitis-causing bacteria. Jpn J Vet Res. 2023;71(3):109–16.
Aishwarya SR, Beena AK, Aparna SV, Archana C, Aysha CH. Antibiogram of Enterococcus sp. isolated from household thayir sample. J Vet Anim Sci. 2024;55(1):57–64. https://doi.org/10.51966/jvas.2024.55.1.57-64
Borowicz M, Krzy?anowska DM, Jafra S. Crystal violet-based assay for the assessment of bacterial biofilm formation in medical tubing. J Microbiol Methods. 2023;204:106656. https://doi.org/10.1016/j.mimet.2022.106656
Venkatramanan M, Sankar GP, Senthil R, Akshay J, Veera RA, Langeswaran K, et al. Inhibition of quorum sensing and biofilm formation in Chromobacterium violaceum by fruit extracts of Passiflora edulis. ACS Omega. 2020;5(40):25605–16. https://doi.org/10.1021/acsomega.0c02483
Ghosh R, Tiwary BK, Kumar A, Chakraborty R. Guava leaf extract inhibits quorum-sensing and Chromobacterium violaceum induced lysis of human hepatoma cells: Whole transcriptome analysis reveals differential gene expression. PLoS ONE. 2014;9(9):e107703. https://doi.org/10.1371/journal.pone.0107703
Vasavi HS, Arun AB, Rekha PD. Inhibition of quorum sensing in Chromobacterium violaceum by Syzygium cumini L. and Pimenta dioica L. Asian Pac J Trop Biomed. 2013;3(12):954–59. https://doi.org/10.1016/S2221-1691(13)60185-9
Mogana R, Adhikari A, Tzar MN, Ramliza R, Wiart C. Antibacterial activities of the extracts, fractions and isolated compounds from Canarium patentinervium Miq. against bacterial clinical isolates. BMC Complement Med Ther. 2020;20(1):55. https://doi.org/10.1186/s12906-020-2837-5
Zhao A, Sun J, Liu Y. Understanding bacterial biofilms: From definition to treatment strategies. Front Cell Infect Microbiol. 2023;13.https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2023.1137947/full
Ashraf JM, Ansari MA, Khan HM, Alzohairy MA, Choi I. Green synthesis of silver nanoparticles and characterization of their inhibitory effects on AGEs formation using biophysical techniques. Sci Rep. 2016;6. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735866/
Asghar MA, Zahir E, Asghar MA, Iqbal J, Rehman AA. Facile, one-pot biosynthesis and characterization of iron, copper and silver nanoparticles using Syzygium cumini leaf extract: As an effective antimicrobial and aflatoxin B 1 adsorption agents. PLoS ONE. 2020;15(7):e0234964. https://doi.org/10.1371/journal.pone.0234964
Ali IAM, Ahmed AB, Al-Ahmed HI. Green synthesis and characterization of silver nanoparticles for reducing the damage to sperm parameters in diabetic compared to metformin. Sci Rep. 2023;13(1):2256. https://doi.org/10.1038/s41598-023-29412-3
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