Assessment of genetic diversity in five populations of Solanum torvum Swartz. from Tripura using SSR and RAPD markers

Authors

  • Mitali Das Cytogenetics and Plant Biotechnology Laboratory, Department of Botany, Tripura University, Suryamaninagar 799 022, Tripura, India
  • H R Singha Cytogenetics and Plant Biotechnology Laboratory, Department of Botany, Tripura University, Suryamaninagar 799 022, Tripura, India
  • Kishan Saha Cytogenetics and Plant Biotechnology Laboratory, Department of Botany, Tripura University, Suryamaninagar 799 022, Tripura, India

DOI:

https://doi.org/10.14719/pst.2020.7.1.650

Keywords:

Genetic diversity, Polymerase Chain Reaction, RAPD, SSR, Solanum torvum

Abstract

Polymerase Chain reaction (PCR) based molecular characterization has been undertaken for assessing the genetic diversity in five populations of Solanum torvum using SSR and RAPD markers. In this study, 8 SSRs produced 151 fragments of which 131 bands were polymorphic (86.38%). The primers, At5 amplified the highest number of polymorphic loci (27) and the highest PIC was recorded in CBT08 (0.54). In comparison, RAPD assay produced 70 bands with 79.16% polymorphism. The PIC value was highest in OPC14 (0.41). UPGMA clustering for SSR and RAPD markers grouped all the populations into two clusters. Our findings on SSR profile suggests that though different populations of S. torvum are inherited from a common ancestor eventually the population (STP5) with greater genetic diversity is stabilized in the high altitude of Sub - Himalayan region of Tripura in the due course of evolution.

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Published

01-01-2020

How to Cite

1.
Das M, Singha HR, Saha K. Assessment of genetic diversity in five populations of Solanum torvum Swartz. from Tripura using SSR and RAPD markers. Plant Sci. Today [Internet]. 2020 Jan. 1 [cited 2024 Nov. 21];7(1):46-54. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/650

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Research Articles