Antidiabetic and antioxidant potential of Zanthoxylum armatum DC. leaves (Rutaceae): An endangered medicinal plant

Authors

  • Sana khan Department of Botany, Panjab University, Chandigarh 160 014, India
  • . Richa Department of Botany, Panjab University, Chandigarh 160 014, India
  • Rinku Jhamta Department of Botany, Panjab University, Chandigarh 160 014, India
  • Harsimran Kaur Department of Botany, Panjab University, Chandigarh 160 014, India

DOI:

https://doi.org/10.14719/pst.2020.7.1.665

Keywords:

Zanthoxylum armatum, antihyperglycemic, DPPH, α-amylase, elemental

Abstract

The present study was designed to evaluate the antidiabetic and antioxidant potential of methanolic extract of Zanthoxylum armatum leaves using in vitro approaches. The concentration of plant extract that inhibited 50% (IC50) of alpha amylase was found to be 89.37±4.68 ?g/ml which is higher than standard. Results of this study shows that 2,2-diphenyl-1-picrylhydrazyl scavenging test show high radical scavenging activity as compared to hydrogen peroxide scanvenging method with IC50 Value of 57.83 ?g/ml and 79.13 ?g/ml, respectively. Plant extract found to exhibit enormous amount of phenols and flavonoid content i.e., 140.71 mg GAE/g and 88.53 mg of Quercetin/g of extract respectively. Further phytochemical analysis revealed that plant exhibit glycosides, alkaloids, terpenoids, flavonoids, saponin and tannin that are frequently implicated as having antidiabetic effects. Elemental analysis revealed the presence of essential elements ‘Mg’, ‘Mn’, ‘Zn’, ‘Fe’, ‘K’, ‘P’, ‘Ca’, ‘Cu’, ‘Mo’ and ‘Ni’ known to play role in regulating blood glucose. It could be speculated that the observed antidiabetic activity of Z. armatum might be related to the presence of these phytochemicals, phenolic compounds as well as mineral elements which found to be the important constituent of Z. armatum. These results indicate that Z. armatum could be an excellent source of natural antioxidants and exhibited antidiabetic activity.

Downloads

Download data is not yet available.

References

1. Srivastava J, Lambert J, Vietmeyer N. Medicinal plants: An expanding role in development. World Bank technical paper. 1996;320. https://doi.org/10.1596/0-8213-3613-4

2. Wild S, Roglic G, Green A, King H, Sicree R. Global Prevalence of Diabetes. Diabetes Care. 2004;27:1047-53. https://doi.org/10.2337/diacare.27.5.1047

3. Giugliano D, Ceriello A, Paolisso G. Oxidative stress and diabetic vascular complications. Diabetes Care. 1996;19:257?67. http://doi.org/10.2337/diacare.19.3.257

4. Sathyanarayana N, Sunitha P, Suresh C, Subramaniam S, Rathinam X. In vitro antioxidant, HPLC profiling of polyphenolic compounds, AAs and FTIR spectrum of Malaysian origin Solanum torvum fruit. Indian Journal of Pharmaceutical Education and Research. 2016;50(2):11-20. https://doi:10.5530/ijper.50.2.13

5. Roy K, Harris F, Dennison SR, Phoenix DA, Singh J. Effects of streptozotocin-induced type 1 diabetes mellitus on protein and ion concentration in ocular tissues of the rat. International Journal of Diabetes and Metabolism. 2005;13:154-58. https://doi.org/10.1196/annals.1372.019

6. Verma N, Khosa RL. Hepatoprotective activity of leaves of Zanthoxylum armatum DC. in CCl4 induce hepatotoxicity in rats. Indian Journal of Biochemistry and Biophysics. 2010;47:124-27. http://nopr.niscair.res.in/handle/123456789/8265

7. Ahmad S, Ali A, Beg H, Dasti AA, Shinwari ZK. Ethnobotanical studies on some medicinal plants of Booni valley, district Chitral, Pakistan. Pakistan Journal of Weed Science Research. 2006;12(3):183-90.

8. Indian Medicinal Plants. An Illustrated Dictionary: Khare; 2007. Springer-Verlag Berlin Heidelberg. p. 730.

9. Samant SS, Butola JS, Sharma A. Assessment of conservation diversity: status and preparation distribution of management plan for medicinal plants in the catchment area of parbati hydroelectric project stage-III in Northwestern Himalaya. Journal of Mountain Science. 2007;4:34–56. https://doi.org/10.1007/s11629-007-0034-3

10. Manu KA, Kuttan G. Anti-metastatic potential of Punarnavine, an alkaloid from Boerhaavia diffusa Linn. The Journal of Immunology. 2009;214:245-55. https://doi.org/10.1016/j.imbio.2008.10.002

11. Njoku OV, Obi C. Phytochemical constituents of some medicinal plants. African Journal of Pure and Applied Chemistry. 2009;3(11):228-33.

12. Siddiqui AA, Ali M. Practical Pharmaceutical Chemistry. 1st ed. New Delhi: CBS Publishers and Distributors; 1997. 126-31.

13. Conforti F, Statti G, Loizzo MR, Sacchetti G, Poli F, Menichini F. In vitro antioxidant effect and inhibition of alpha-amylase of two varieties of Amaranthus caudatus seeds. Biological and Pharmaceutical Bulletin. 2005;28:1098-102. http://doi.org/10.1248/bpb.28.1098

14. Harini R, Sindhu S, Sagadevan E, Arumugam P. Characterization of in vitro antioxidant potential of Azadirachta indica and Abutilon indicum by different assay methods. Journal of Pharmacy Researc­­­h. 2012;5:3227-31.

15. Jayaprakasha GK, Lingamallu JR, Kunnumpurath KS. Antioxidant activities of flavidin in different in vitro model system. Bioorganic & Medicinal Chemistry. 2004;12:5141-46. http://doi.org/10.1016/j.bmc.2004.07.028

16. Habila JD, Bello IA, Dzikwi AA, Musa H, Abubakar N. Total phenolics and antioxidant activity of Tridax procumbens Linn. African Journal of Pharmacy and Pharmacology. 2010;4(3):123-26.

17. Chang C, Yang M, Wen H, Chern, J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food Drug Analysis. 2002;10:178-82.

18. Okwu DE, Okwu ME. Chemical composition of Spondias mombin Linn. plant parts. Journal of Sustainable Agriculture and Environment. 2004;6:140-47. https://doi.org/10.1080/0972-060X.2003.10643343

19. Ebrahimzadeh MA, Nabavi SF, Nabavi SM. Antioxidant activities of methanol extract of Sambucus ebulus L. flower.  Pakistan Journal of Biological Sciences. 2001;12:447-50. http://doi.org/10.3923/pjbs.2009.447.450

20. Khan S, Richa, Kaur H and Jhamta R. Evaluation of antioxidant potential and phytochemical characterization using GCMS analysis of bioactive compounds of Achillea filipendulina (L.) Leaves. Journal of Pharmacognosy and Phytochemistry. 2019;8(3):258-65.

21. Momeni J, Ntchatchoua PD, Fadimatou, Akam MT, Ngassoum MB. Antioxidant Activities of Some Cameroonian Plants Extracts Used in the Treatment of Intestinal and Infectious Diseases. Indian Journal of Pharmaceutical Sciences. 2010;72:140-44. https://dx.doi.org/10.4103%2F0250-474X.62236

22. Sabu MC, Kuttan R. Anti-diabetic activity of medicinal plants and its relationship with their antioxidant property. Journal of Ethnopharmacology. 2002;81:155-60. http://doi.org/10.1016/s0378-8741(02)00034-x

23. Kobrin SM, Goldfarb S. Magnesium deficiency, Seminar in Nephrology. 1990;10: 525–35.

24. Nguyen TQ, Maalouf NM, Sakhaee K. Comparison of insulin action on glucose versus potassium uptake in humans. Clinical Journal of the American Society of Nephrology. 2011;6(7):1533-39. https://doi.org/10.2215/CJN.00750111

25. Fang L, Li X. Level of serum phosphorus and adult type 2 diabetes mellitus. Journal of Central South University. 2016;41(5):502-06.https://doi.org/10.11817/j.issn.1672-7347.2016.05.009

26. Khaw KT, Barrett-Connor E. Dietary potassium and blood pressure in a population. The American Journal of Clinical Nutrition. 1984;39:963-68. https://doi.org/10.1093/ajcn/39.6.963

27. Jansen J, Karges W, Rink L. Zinc and diabetes—clinical links and molecular mechanisms. The Journal of Nutritional Biochemistry. 2009;20:399–417. https://doi.org/10.1016/j.jnutbio.2009.01.009

28. Hussain FM, Arif Maan MA, Sheikh H, Nawaz AJ. Trace elements status in type 2 diabetes. Bangladesh journal of medical science. 2009;8(3):12-17. https://doi.org/10.3329/bjms.v8i3.3983

29. Rajendran A, Narayanan V, Gnanave I. Study on the Analysis of Trace Elements in Aloe vera and Its Biological Importance. Journal of Applied Sciences Research. 2007; 3(11):1476-78.

30. Guimaraes MM, Carvalho AC, Silva MS. Effect of chromium supplementation on the glucose homeostasis and anthropometry of type 2 diabetic patients: double blind, randomized clinical trial. Journal of Trace Elements in Medicine and Biology. 2016;36:65-72. http://dx.doi.org/10.1016/j.jtemb.2016.04.002

31. Fillat C, Rodríguez-Gil JE, Guinovart JJ. Molybdate and tungstate act like vanadate on glucose metabolism in isolated hepatocytes. Comparison of insulin action on glucose versus potassium uptake in humans. Biochem Journal. 1992;282(3):659-63. https://dx.doi.org/10.2215%2FCJN.00750111

Downloads

Published

12-01-2020

How to Cite

1.
khan S, Richa ., Jhamta R, Kaur H. Antidiabetic and antioxidant potential of Zanthoxylum armatum DC. leaves (Rutaceae): An endangered medicinal plant. Plant Sci. Today [Internet]. 2020 Jan. 12 [cited 2024 Nov. 4];7(1):93-100. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/665

Issue

Section

Research Articles