Ozonated water treatment for sustaining quality attributes and prolonging shelf life in grapes

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DOI:

https://doi.org/10.14719/pst.6920

Keywords:

grapes, ozonated water, postharvest, quality, shelf life

Abstract

Grapes (Vitis vinifera), a subtropical fruit crop, are highly valued for their nutritional composition and global economic importance, both as fresh consumption and processed forms. However, postharvest quality loss, primarily caused by grey mould (Botrytis cinerea), microbial decay and physiological weight loss, poses a significant challenge to grape storage and marketability. This experiment was designed to evaluate the efficacy of postharvest ozonated water treatment on quality attributes and extending the shelf life of grapes. Grape bunches were subjected to four treatments: control (no dip) and immersion in 0.3 ppm ozonated water for 5, 10 and 15 min, followed by storage at 4±2°C and 90 % relative humidity (RH). Results indicated significant improvements in key quality parameters for grapes treated with ozonated water, particularly with the 15 min treatment (T4). These bunches exhibited higher titratable acidity (0.24 %), ascorbic acid content (1.81 mg/100 g), and firmness (4.80 N) while exhibiting 21.82 % lower physiological weight loss compared to the control. Ozonated water treatment also minimized berry abscission (3.77 %) and maintained sensory properties. The sugar-acid ratio was highest (84.72) in 5 min treatment group (T2) on the 36th day of storage, enhancing flavour attributes. Significantly, T4 extended the shelf life of grapes to 39.84 days, nearly five days longer than the untreated grapes. This study demonstrated the potential of ozonated water as an eco-friendly, residue-free technology for postharvest preservation, offering a safer alternative to chemical fumigation. The findings support the integration of ozonated water treatments into grape postharvest management practices to enhance quality, storability and consumer acceptability.

 

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References

Hussain SZ, Naseer B, Qadri T, Fatima T, Bhat TA, editors. Grapes (Vitis vinifera)—morphology, taxonomy, composition and health benefits. In: Fruits grown in highland regions of the Himalayas: nutritional and health benefits. Cham: Springer; 2021. p. 103–15. https://doi.org/10.1007/978-3-030-75502-7

Area, production and productivity of grapes in India. Indiastat [Internet]. 2023–24 [cited 2025 Jan 3;]. Available from: https://www.indiastat.com/data/agriculture/grapes-viticulture.

Agriexcgange. APEDA [Internet]. 2023–24 [cited 2025 Jan 09]. Available from: https://apeda.gov.in/Grapes

Zhao Y, Jin Q, Wang ZJ, Tao XY, Luo XD. Quality assurance of postharvest grapes against Botrytis cinerea by terbinafine. Nat Prod Biopros. 2023;13(1):25. https://doi.org/10.1007/s13659-023-00389-w

Rivera SA, Zoffoli JP, Latorre BA. Determination of optimal sulfur dioxide time and concentration product for postharvest control of gray mold of blueberry fruit. Postharvest Biol and Techno. 2013;83:40–46. https://doi.org/10.1007/s13659-023-00389-w10.1016/j.postharvbio.2013.03.007

Jiang Y, Zhang L, Li X, Chen L, Yuan J, Wang H, et al. Preharvest fungicide treatments reduce the effective SO2 threshold of postharvest fumigation to control pathogens and maintain quality of red globe (Vitis vinifera) grapes. J Food Safety. 2023;43(4):e13047. https://doi.org/10.1111/jfs.13047

Wen LZ, Ping ZP, Feng HY, Ming CM, Qiang ZZ. Effect of sulfur dioxide injury on aroma components of postharvest red globe. Acta Botanica Boreali-Occidentalia Sinica. 2011;31(2):385–92.

Lemic D, Galeši? MA, Bjeliš M, Gasparic VH. Ozone treatment as a sustainable alternative for suppressing blue mold in mandarins and extending shelf life. Agriculture. 2024;14(7):1196. https://doi.org/10.3390/agriculture14071196

Graham D. Use of ozone for food processing. Food Techno. 1997;51(6):72–75.

Nwaiwu O, Ibekwe VI. Optimization of ozone decomposition time and its effect on physicochemical and bacteriological quality of table water. Croat J Food Sci Tech. 2019;11(1):131–34. https://doi.org/10.17508/cjfst.2019.11.1.07

Prabha V, Barma RD, Singh R, Madan A. Ozone technology in food processing: A review. Tre Biosci. 2015;8(16):4031–47.

Palou L, Crisosto CH, Smilanick JL, Adaskaveg JE, Zoffoli JP. Effects of continuous 0.3 ppm ozone exposure on decay development and physiological responses of peaches and table grapes in cold storage. Postharvest Biol Techno. 2002;24(1):39–48. https://doi.org/10.1016/S0925-5214(01)00118-1

Ranganna S. Handbook of analysis and quality control for fruit and vegetable products. New Delhi: Tata McGraw-Hill Education; 1986.

Somogyi M. Notes on sugar determination. J Biol Chem. 1952;195(1):19–23. https://doi.org/10.1016/S0021-9258(19)50870-5

Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viti. 1965;16(3):144–58. https://doi.org/10.5344/ajev.1965.16.3.144

Ranganna S. Manual of analysis of fruit and vegetable products. New Delhi: Tata McGraw-Hill; 1977.

Cynkar W, Cozzolino D, Dambergs B, Janik L, Gishen M. The effects of homogenization method and freezing on the determination of quality parameters in red grape berries of Vitis vinifera. Aust J Grape Wine Res. 2008;10:236–42. https://doi.org/10.1111/j.1755-0238.2004.tb00027.x

AOAC. Official methods of analysis Fifteenth edition. Washington: D.C. Association of Official Analytical Chemists; 2001.

Heleno FF, de Queiroz ME, Neves AA, Faroni LR, Sousa FAd, Oliveira AFd. Ozone treatment for the removal of residual chlorothalonil and effects on the quality of table grapes. J Brazil Chem Soc. 2015;26:687–94. https://doi.org/10.5935/0103-5053.20150027

Li C, Wang S, Wang J, Wu Z, Xu Y, Wu Z. Ozone treatment promotes physicochemical properties and antioxidant capacity of fresh-cut red pitaya based on phenolic metabolism. Front Nutr. 2022;9:1016607. https://doi.org/10.3389/fnut.2022.1016607

Ullrich L, Gillich E andré A, Panarese S, Imhaus AF, Fieseler L, Chetschik I. Influence of ozone treatment during storage on odour-active compounds, total titratable acidity and ascorbic acid in oranges and bananas. App Sci. 2023;13(19):10885. https://doi.org/10.3390/app131910885

Morais ML, Alvinhão JEO, Franco DV, Silva EDB, Pinto NAVD. Application of ozone aiming to keep the quality of strawberries using a low cost reactor. Revista Brasileira de Fruticultura. 2015;37(3):559–67. https://doi.org/10.1590/0100-2945-181/14

Cao S, Meng L, Ma C, Ba L, Lei J, Ji N, Wang R. Effect of ozone treatment on physicochemical parameters and ethylene biosynthesis inhibition in Guichang kiwifruit. Food Sci Techno. 2021;42:e64820. https://doi.org/10.1590/fst.64820

Vettraino AM, Vinciguerra V, Pacini G, Forniti R, Goffi V, Botondi R. Gaseous ozone as a suitable solution for postharvest chestnut storage: evaluation of quality parameter trends. Food Biopro Technol. 2020;13:187–93. https://doi.org/10.1007/s11947-019-02378-9

Yilmaz T, Ates F, Turan M, Hatterman-Valenti H, Kaya O. Dynamics of sugars, organic acids, hormones and antioxidants in grape varieties Italia and Bronx seedless during berry development and ripening. Horticulturae. 2024;10(3):229. https://doi.org/10.3390/horticulturae10030229

Kumar S, Abedin MM, Singh AK, Das S. Role of phenolic compounds in plant-defensive mechanisms. In: Lone R, Shuab R, Kamili A, editors. Plant phenolics in sustainable agriculture. Singapore: Springer; 2020. p. 517–32. https://doi.org/10.1007/978-981-15-4890-1_22

Shahab M, Roberto SR, Adnan M, Fahad S, Koyama R, Saleem MH, et al. Phenolic compounds as a quality determinant of grapes: A critical review. J Pl Growth Regu. 2023;42(9):5325–31. https://doi.org/10.1007/s00344-023-10953-w

Gorzelany J, Kapusta I, Pluta S, Belcar J, Pento? K, Basara O. Effect of gaseous ozone and storage time on polyphenolic profile and sugar content in fruits of selected Vaccinium corymbosum L. genotypes. Molecules. 2023;28(24):8106. https://doi.org/10.3390/molecules28248106

Artés-Hernández F, Aguayo E, Artés F, Tomás-Barberán F. Enriched ozone atmosphere enhances bioactive phenolics in seedless table grapes after prolonged shelf life. J Sci Food Agric. 2007;87:824–31. https://doi.org/10.1002/jsfa.2780

Ren J, Li X, Dong C, Zheng P, Zhang N, Ji H, et al. Effect of ozone treatment on phenylpropanoid metabolism in harvested cantaloupes. J Food Sci. 2024;89(8):4914–25. https://doi.org/10.1111/1750-3841.17234

Gao C-c, Lin Q, Dong C-h, Ji H-p, Yu J-z, Chen C-k, et al. Effects of ozone concentration on the postharvest quality and microbial diversity of Muscat Hamburg grapes. RSC Adv. 2020;10(15):9037–45. https://doi.org/10.1039/c9ra10479h

Admane N, Genovese F, Altieri G, Tauriello A, Trani A, Gambacorta G, Renzo G. Effect of ozone or carbon dioxide pre-treatment during long-term storage of organic table grapes with modified atmosphere packaging. LWT Food Sci Techno. 2018;98:170–78. https://doi.org/10.1016/j.lwt.2018.08.041

Ku?niar P, Belcar J, Zardzewia?y M, Basara O, Gorzelany J. Effect of ozonation on the mechanical, chemical and microbiological properties of organically grown red currant (Ribes rubrum L.) fruit. Molecules. 2022;27(23):8231. https://doi.org/10.3390/molecules27238231

Nurzakiyyah N, Prihastanti E, Hastuti E, Dea M. Effect of ozone treatment on vitamin C levels of strawberry (Fragaria × ananassa) with different storage temperatures. Int J Hortic Agric Food Sci. 2022;6:1–4. https://doi.org/10.22161/ijhaf.6.1.1

Wang Y, Li Y, Yang S, Wu Z, Shen Y. Gaseous ozone treatment prolongs the shelf-life of fresh-cut kiwifruit by maintaining its ascorbic acid content. LWT. 2022;172:114196. https://doi.org/10.1016/j.lwt.2022.114196

Chen C, Zhang X, Zhang H, Ban Z, Li L, Dong C, et al. Label-free quantitative proteomics to investigate the response of strawberry fruit after controlled ozone treatment. RSC Adv. 2019;9(2):676–89. https://doi.org/10.1039/c8ra08405j

Kim JJ, Fan R, Allison LK, Andrew TL. On-site identification of ozone damage in fruiting plants using vapor-deposited conducting polymer tattoos. Sci Adv. 2020;6(36):eabc3296. https://doi.org/10.1126/sciadv.abc3296

Díaz-López M, Galera L, Bastida F, Nicolás E. Tomato growth and physiology as well as soil physicochemical and biological properties affected by ozonated water in a saline agroecosystem. Sci Tot Environ. 2024;906:167472. https://doi.org/10.1016/j.scitotenv.2023.167472

Liu C, Ma T, Wenzhong h, Tian M, Sun L. Effects of aqueous ozone treatments on microbial load reduction and shelf life extension of fresh-cut apple. Int J Food Sci Techno. 2016;51:1099–109. https://doi.org/10.1111/ijfs.13078

Peng X, Dong C, Zhang N, Zheng P, Bai Y, Ji H, et al. Effect of ozone treatment on the decay and cell wall metabolism during the postharvest storage of cantaloupe. Sci Hortic. 2024;331:113119. https://doi.org/10.1016/j.scienta.2024.113119

Piechowiak T, Migut D, Józefczyk R, Balawejder M. Ozone treatment improves the texture of strawberry fruit during storage. Antioxidants. 2022;11(5):821. https://doi.org/10.3390/antiox11050821

Pandiselvam R, Singh A, Agriopoulou S, Sachadyn-Król M, Aslam R, Lima CMG, et al. A comprehensive review of impacts of ozone treatment on textural properties in different food products. Tre Food Sci Techno. 2022;127:74–86. https://doi.org/10.1016/j.tifs.2022.06.008

Kampf CJ, Liu F, Reinmuth-Selzle K, Berkemeier T, Meusel H, Shiraiwa M, Pöschl U. Protein cross-linking and oligomerization through dityrosine formation upon exposure to ozone. Environ Sci Techno. 2015;49(18):10859–66. https://doi.org/10.1021/acs.est.5b02902

Campayo A, Savoi S, Romieu C, López-Jiménez AJ, Hoz SDLK, Salinas MR, et al. The application of ozonated water rearranges the Vitis vinifera L. leaf and berry transcriptomes eliciting defence and antioxidant responses. Sci Rep. 2021;11(1):8114. https://doi.org/10.1038/s41598-021-87542-y

Modesti M, Macaluso M, Taglieri I, Bellincontro A, Sanmartin C. Ozone and bioactive compounds in grapes and wine. Foods. 2021;10(12):2934. https://doi.org/10.3390/foods10122934

Guo C, Wang X, Wang Q, Zhao Z, Xie B, Xu L, Zhang R. Plant defense mechanisms against ozone stress: Insights from secondary metabolism. Environ Exp Bot. 2024;217:105553. https://doi.org/10.1016/j.envexpbot.2023.105553

Maciej B, Natalia M, Wioletta S, Natalia K, Tomasz P, Anita Z. Effect of two types of ozone treatments on the quality of apple fruits. Acta Universitatis Cibiniensis Series E: Food Techno. 2021;25(2):285–92. https://doi.org/10.2478/aucft-2021-0026

Panou A, Karabagias I, Riganakos K. The effect of different gaseous ozone treatments on physicochemical characteristics and shelf life of apricots stored under refrigeration. J Food Process Preserv. 2018;42. https://doi.org/10.1111/jfpp.13614

Published

21-02-2025 — Updated on 07-03-2025

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1.
Chinnasamy K, Balachandran A, Borthakur P, Venkatachalam S, Kolandasamy P. Ozonated water treatment for sustaining quality attributes and prolonging shelf life in grapes. Plant Sci. Today [Internet]. 2025 Mar. 7 [cited 2025 Mar. 30];12(1). Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/6920

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Research Articles