A comparative study of cellulase production in inorganic and organic supplements by a cellulolytic tree bark fungus
DOI:
https://doi.org/10.14719/pst.2020.7.2.708Keywords:
sugarcane bagasse, organic supplements, cellulase, inorganic supplementsAbstract
The study was conducted to compare the cellulase production using organic and inorganic supplements by isolating a tree bark (Mangifera indica) fungus, for a cost-effective cellulase production. Three fungi (f1, f2 and f3) were isolated from the bark of the tree, of which f3 was found to be potential in cellulase production which was confirmed by primary screening (congo red activity staining). Through endoglucanase and total cellulase activity assays, it was observed that the isolated strain exhibited cellulase activity of 0.25 U/ml for endoglucanase when 1% CMC was supplemented to the medium. An activity of 8 IU/g towards FPase and for endoglucanase 10.35 U/g was obtained on non-purified inorganic supplements. Sugarcane bagasse was found to be the best inorganic supplement for cellulase production. Here, we try to adopt a cost-effective production strategy of cellulase. The future perspective of this investigation is to identify the strain and purification of the enzyme for industrial purpose.
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References
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32. Xia L, Cen P. Cellulase production by solid state fermentation on lignocellulosic waste from the xylose industry. Process Bichem. 1999;34:909-12. https://doi.org/10.1016/S0032-9592(99)00015-1
33. Sanjay KHK, Sharma B, Sarkar C. Effect of substrate and fermentation conditions on pectinase and cellulase production by Aspergillus niger NCIM 548 in Submerged (SmF) and Solid State Fermentation (SSF). Food Sci Biotechnol. 2011;20(5): 1289-98. https://doi.org/10.1007/s10068-011-0178-3
34. Sherief AA, El-Tanash AB, Atia N. Cellulase production by Aspergillus fumigatus grown on mixed substrate of rice straw and wheat bran. Research Journal of Microbiology. 2010;5(3):199-211. https://doi.org/10.3923/jm.2010.199.211
2. Murai T, Ueda M, Kavaguchi T, Arai M, Tanaka M. Assimilation of cellooligosaccharides by a cell surface engineered yeast expressing ?-glucosidase and carboxymethylcellulase from Aspergillus aculeatus. Appl Environ. Microbiol. 1998;64 (12):4857-61. https://doi.org/10.1128/AEM.64.12.4857-4861.1998
3. Sukumaran RK, Singhania RR, Pandey A. Microbial cellulases Production, applications and challenges. Journal of Scientific & Industrial Research. 2005;64:832-44
4. Fayyaz UR, Mehwish AM, Ilyas T, Ashraf S, Amtul JS, Naima HN et al. Isolation of cellulolytic activities from Tribolium castaneum (red flour beetle) Agricultural and Biological Chemistry. 2009;8(23):6710-671
5. Akiba S, Kimura Y, Yamamoto K, Kumagai H. Purification and characterization of a protease-resistant cellulase from Aspergillus niger. J Ferment Bioeng. 1995;79:125-30. https://doi.org/10.1016/0922-338X(95)94078-6
6. Paice MG, Jurasek L. Removing hemicellulose from pulps by specific enzymic hydrolysis. J Wood Chem Tech. 1987;4:187-98. https://doi.org/10.1080/02773818408081152
7. Poutanen K. Enzymes: An important tool in the improvement of the quality of cereal foods. Trends in Food Science and Technology. 1997;8:300-06. https://doi.org/10.1016/S0924-2244(97)01063-7
8. Shikata S, Saeki K, Okoshi H, Yoshimatsu T, Ozaki K, Kawai S. Alkaline Cellulases for Laundry Detergents: Production by Alkalophilic Strains of Bacillus and Some Properties of the Crude Enzymes Agric. Biol. Chem. 1990;54(1):91-96. https://doi.org/10.1271/bbb1961.54.91
9. Ajoy KS, Nolan JE. Enzymatic Hydrolysis of Cotton Fibers. Modeling Using an Empirical Equation. The Journal of Cotton Science. 2004;8:254–60.
10. Artur CP. Mechanism of action in textile process. Carbohydrate Polymers. 1998;37:273-77. https://doi.org/10.1016/S0144-8617(98)00070-8
11. Bajpai P. Application of enzymes in the pulp and paper industry. Biotechnology Progress. 1999;15:147-57. https://doi.org/10.1021/bp990013k
12. Bhat MK. Cellulases and related enzymes in biotechnology. Biotechnology Advances. 2000;18:355-83. https://doi.org/10.1016/S0734-9750(00)00041-0
13. Sadhu S, Maiti TK. Cellulase Production by Bacteria: A Review British Microbiology Research Journal. 2013;3(3):235-58. https://doi.org/10.9734/BMRJ/2013/2367
14. Miyamoto K, editor. Renewable biological systems for alternative sustainable energy production. Food & Agriculture Org.; 1997
15. Rubia CGC, Sandro AR, Thatiane RM, JoaoLucio A, Joao AP, Cristina GM, et al. Endophytic fungi: expanding the arsenal of industrial enzyme producers. J Ind Microbiol Biotechnol. 2014; 41:1467-78. https://doi.org/10.1007/s10295-014-1496-2
16. Harinder SO, Yogita C, Sunil B, Gurpreet SD. Production of cellulases through solid state fermentation using kinnow pulp as a major substrate, Food Bioprocess Technol. 2010;3:528-36. https://doi.org/10.1007/s11947-008-0092-8
17. Saithong P, Panthavee W, Stonsavapak S, Congfa L. Isolation and Primary identification of endophytic fungi from Cephalotaxus mannii trees. Maejoint J Sci Technol. 2010;4(3);446-53
18. Pointing SB. Qualitative methods for the determination of lignocellulolytic enzyme production by tropical fungi. Fungal Diversity. 1999;2:17-33
19. Yeoh HH, Khew E, Lim G. A simple method for screening cellulolytic fungi. Mycologica. 1985;77:161-62. https://doi.org/10.1080/00275514.1985.12025077
20. Ghose TK. Measurement of cellulase activity. Pure and Applied Chemistry. 1987;59(2):257-68. https://doi.org/10.1351/pac198759020257
21. Alonso R, Tiscornia S, Alfenas AC, Bettucci L. Fungi associated to bark lesions of Eucalyptus globulus stems in plantations from Uruguay. R Árvore. 2009;33:(4)591-97. https://doi.org/10.1590/S0100-67622009000400001
22. Malcolm S, Cumbie R. Congo red as a fluorochrome for the rapid detection of fungi. Journal of Clinical Microbiology. 1988; 26(5):827-30. https://doi.org/10.1128/JCM.26.5.827-830.1988
23. Lillie RD. Diazo and polyazo dyes. In: EH Stotz, VM Emmel, editors. HJ Conn's biological stains. The Williams & Wilkins Co. Baltimore; 1977. Vol. 9. pp. 147-48
24. Wood PJ, Fulcher RG. Interaction of some dyes with cereal glucans. Cereal Chem. 1978;55:952-66
25. Meddeb-Mouelhi F, Moisana JK, Beauregarda M. A comparison of plate assay methods for detecting extracellular cellulase and xylanase activity. Enzyme and Microbial Technology. 2014; 66:16-19. https://doi.org/10.1016/j.enzmictec.2014.07.004
26. Uttam K, Tapwal A, Kalkal P, Varghese S, Chandra S. Isolation and screening of cellulase producing fungi from forest waste. International Journal of Pharmaceutical and Biological Archives. 2013;5(1):56–59
27. Om S, Shrivastava MK, Anoop B, Tripathi V, Gupta V, Kuldeep K. Production, Purification, Characterization and Application of Cellulase. Int J Pure App Biosci. 2014;2(4):222-39
28. Sajith S, Sreedevi S, Priji P, Unni KN, Benjamin S. Production and partial purification of cellulase from a novel fungus, Aspergillus flavus BS1. Ann Microbiol. 2014;64:763-71. https://doi.org/10.1007/s13213-013-0711-0
29. Jun H, Bing Y, Keying Z, Xuemei D, Daiwen C. Strain improvement of Trichoderma reesei Rut C-30 for increased cellulase production. Indian J Microbiol. 2009;49:188-95. https://doi.org/10.1007/s12088-009-0030-0
30. Chatterjee R, Majumder K, Sengupta S. Tamarind kernel powder co-induces xylanase and cellulase production during submerged fermentation of Termitomyces clypeatus. Biotechnology and Bioprocess Engineering. 2010;15:854-61. https://doi.org/10.1007/s12257-009-3042-z
31. Muhammad S, Aqeel A, Shakeel AK. Production of cellulase from Aspergillus terreus MS105 on crude and commercially purified substrates. 3Biotech. 2016;6:103. https://doi.org/10.1007/s13205-016-0420-z
32. Xia L, Cen P. Cellulase production by solid state fermentation on lignocellulosic waste from the xylose industry. Process Bichem. 1999;34:909-12. https://doi.org/10.1016/S0032-9592(99)00015-1
33. Sanjay KHK, Sharma B, Sarkar C. Effect of substrate and fermentation conditions on pectinase and cellulase production by Aspergillus niger NCIM 548 in Submerged (SmF) and Solid State Fermentation (SSF). Food Sci Biotechnol. 2011;20(5): 1289-98. https://doi.org/10.1007/s10068-011-0178-3
34. Sherief AA, El-Tanash AB, Atia N. Cellulase production by Aspergillus fumigatus grown on mixed substrate of rice straw and wheat bran. Research Journal of Microbiology. 2010;5(3):199-211. https://doi.org/10.3923/jm.2010.199.211
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Published
01-05-2020
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Vijeetha V, Nair AJ. A comparative study of cellulase production in inorganic and organic supplements by a cellulolytic tree bark fungus. Plant Sci. Today [Internet]. 2020 May 1 [cited 2024 Dec. 22];7(2):227-32. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/708
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